Response properties of mechanoreceptors and nociceptors in mouse glabrous skin: an in vivo study.

Abstract:

:The increasing use of transgenic mice for the study of pain mechanisms necessitates comprehensive understanding of the murine somatosensory system. Using an in vivo mouse preparation, we studied response properties of tibial nerve afferent fibers innervating glabrous skin. Recordings were obtained from 225 fibers identified by mechanical stimulation of the skin. Of these, 106 were classed as A beta mechanoreceptors, 51 as A delta fibers, and 68 as C fibers. A beta mechanoreceptors had a mean conduction velocity of 22.2 +/- 0.7 (SE) m/s (13.8--40.0 m/s) and a median mechanical threshold of 2.1 mN (0.4--56.6 mN) and were subclassed as rapidly adapting (RA, n = 75) or slowly adapting (SA, n = 31) based on responses to constant force mechanical stimuli. Conduction velocities ranged from 1.4 to 13.6 m/s (mean 7.1 +/- 0.6 m/s) for A delta fibers and 0.21 to 1.3 m/s (0.7 +/- 0.1 m/s) for C fibers. Median mechanical thresholds were 10.4 and 24.4 mN for A delta and C fibers, respectively. Responses of A delta and C fibers evoked by heat (35--51 degrees C) and by cold (28 to -12 degrees C) stimuli were determined. Mean response thresholds of A delta fibers were 42.0 +/- 3.1 degrees C for heat and 7.6 +/- 3.8 degrees C for cold, whereas mean response thresholds of C fibers were 40.3 +/- 0.4 degrees C for heat and 10.1 +/- 1.9 degrees C for cold. Responses evoked by heat and cold stimuli increased monotonically with stimulus intensity. Although only 12% of tested A delta fibers were heat sensitive, 50% responded to cold. Only one A delta nociceptor responded to both heat and cold stimuli. In addition, 40% of A delta fibers were only mechanosensitive since they responded neither to heat nor to cold stimuli. Thermal stimuli evoked responses from the majority of C fibers: 82% were heat sensitive, while 77% of C fibers were excited by cold, and 68% were excited by both heat and cold stimuli. Only 11% of C fibers were insensitive to heat and/or cold. This in vivo study provides an analysis of mouse primary afferent fibers innervating glabrous skin including new information on encoding of noxious thermal stimuli within the peripheral somatosensory system of the mouse. These results will be useful for future comparative studies with transgenic mice.

journal_name

J Neurophysiol

authors

Cain DM,Khasabov SG,Simone DA

doi

10.1152/jn.2001.85.4.1561

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

1561-74

issue

4

eissn

0022-3077

issn

1522-1598

journal_volume

85

pub_type

杂志文章
  • Forebrain pathway for auditory space processing in the barn owl.

    abstract::The forebrain plays an important role in many aspects of sound localization behavior. Yet, the forebrain pathway that processes auditory spatial information is not known for any species. Using standard anatomic labeling techniques, we used a "top-down" approach to trace the flow of auditory spatial information from an...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1998.79.2.891

    authors: Cohen YE,Miller GL,Knudsen EI

    更新日期:1998-02-01 00:00:00

  • Alteration of the mu opioid receptor: Ca2+ channel signaling pathway in a subset of rat sensory neurons following chronic femoral artery occlusion.

    abstract::The exercise pressor reflex, a crucial component of the cardiovascular response under physiological and pathophysiological states, is activated via metabolic and mechanical mediators that originate from contracting muscles and stimulate group III and IV afferents. We reported previously that stimulation of mu opioid r...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00630.2014

    authors: Hassan B,Kim JS,Farrag M,Kaufman MP,Ruiz-Velasco V

    更新日期:2014-12-15 00:00:00

  • Local field potential oscillations in primate cerebellar cortex during voluntary movement.

    abstract::Sustained oscillations of 13-18 Hz were observed in local field potentials (LFPs) in the cerebellar cortex of a behaving monkey. These oscillations, which appeared to be generated in the granular cell layer, were particularly prominent in the paramedian lobule. The oscillatory activity decreased during drowsiness or e...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.78.6.3502

    authors: Pellerin JP,Lamarre Y

    更新日期:1997-12-01 00:00:00

  • Modulation of membrane potential in mesothoracic moto- and interneurons during stick insect front-leg walking.

    abstract::During walking, maintenance and coordination of activity in leg motoneurons requires intersegmental signal transfer. In a semi-intact preparation of the stick insect, we studied membrane potential modulations in mesothoracic (middle leg) motoneurons and local premotor nonspiking interneurons that were induced by stepp...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00493.2005

    authors: Ludwar BCh,Westmark S,Büschges A,Schmidt J

    更新日期:2005-10-01 00:00:00

  • Mechanisms of depolarizing inhibition at the crayfish giant motor synapse. I. Electrophysiology.

    abstract::1. Mechanisms of depolarizing synaptic inhibition were investigated at the crayfish giant motor synapse with the use of two-electrode current- and voltage-clamp techniques. Depolarizing inhibitory postsynaptic potentials (d-IPSPs) of between 5 and 15 mV in amplitude are produced there in the motor giant motoneuron (Mo...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1990.64.2.532

    authors: Edwards DH

    更新日期:1990-08-01 00:00:00

  • Distribution of functional glutamate and GABA receptors on hippocampal pyramidal cells and interneurons.

    abstract::The distribution of functional neurotransmitter receptors is an important determinant of neuronal information processing. To map the location of functional glutamate and GABA receptors on individual hippocampal neurons, we photolyzed "caged" glutamate and GABA while measuring the electrical currents resulting from act...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.84.1.28

    authors: Pettit DL,Augustine GJ

    更新日期:2000-07-01 00:00:00

  • Reflex responses induced by tooth unloading.

    abstract::The reflex response of the masseter muscle to the rapid unloading of a single maxillary incisor tooth was studied. Unloading of a static force of 2 N in the horizontal direction resulted in a short-latency excitation, inhibition, and long-latency excitation of masseter muscle activity occurring at latencies of approxi...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章

    doi:10.1152/jn.2000.84.2.1088

    authors: Türker KS,Jenkins M

    更新日期:2000-08-01 00:00:00

  • Analysis of temporal discharge characteristics of dorsal cochlear nucleus neurons of unanesthetized decerebrate cats.

    abstract::1. We examined the mean and standard deviation (SD) of interspike intervals (ISI) and the coefficient of variation (CV, the ratio of SD of ISI to mean ISI) of ISIs versus time to study discharge regularity of units in the dorsal cochlear nucleus (DCN) of decerebrate unanesthetized cats. The units were characterized by...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.67.5.1247

    authors: Parham K,Kim DO

    更新日期:1992-05-01 00:00:00

  • Generalizing movement patterns following shoulder fixation.

    abstract::Generalizing newly learned movement patterns beyond the training context is challenging for most motor learning situations. Here we tested whether learning of a new physical property of the arm during self-initiated reaching generalizes to new arm configurations. Human participants performed a single-joint elbow reach...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00696.2019

    authors: Maeda RS,Zdybal JM,Gribble PL,Pruszynski JA

    更新日期:2020-03-01 00:00:00

  • Hyperpolarization-activated current (Ih) in the inferior colliculus: distribution and contribution to temporal processing.

    abstract::Neurons in the inferior colliculus (IC) process acoustic information converging from inputs from almost all nuclei of the auditory brain stem. Despite its importance in auditory processing, little is known about the distribution of ion currents in IC neurons, namely the hyperpolarization-activated current Ih. This cur...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00375.2003

    authors: Koch U,Grothe B

    更新日期:2003-12-01 00:00:00

  • Rapid acquisition of novel interface control by small ensembles of arbitrarily selected primary motor cortex neurons.

    abstract::Pioneering studies demonstrated that novel degrees of freedom could be controlled individually by directly encoding the firing rate of single motor cortex neurons, without regard to each neuron's role in controlling movement of the native limb. In contrast, recent brain-computer interface work has emphasized decoding ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00373.2013

    authors: Law AJ,Rivlis G,Schieber MH

    更新日期:2014-09-15 00:00:00

  • The open- and closed-loop gain-characteristics of the cone/horizontal cell synapse in goldfish retina.

    abstract::Under constant light-adapted conditions, vision seems to be rather linear. However, the processes underlying the synaptic transmission between cones and second-order neurons (bipolar cells and horizontal cells) are highly nonlinear. In this paper, the gain-characteristics of the transmission from cones to horizontal c...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.84.3.1256

    authors: Kraaij DA,Spekreijse H,Kamermans M

    更新日期:2000-09-01 00:00:00

  • Generation and coordination of heartbeat timing oscillation in the medicinal leech. II. Intersegmental coordination.

    abstract::1. A network of four pairs of interneurons, HN(1)-HN(4), times the heartbeat of the medicinal leech. The following model accounts for the stable phase relationships in the network and the network's response to perturbation, entrainment, and stimulation. 2. There are two centers of oscillation, one the HN(3) pair in th...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1983.49.3.627

    authors: Peterson EL

    更新日期:1983-03-01 00:00:00

  • Mechanosensitive potassium channels in rat colon sensory neurons.

    abstract::Single-channel recording techniques were used to characterize mechanosensitive channels in identified (1.1'-dioctadecyl-3,3,3', 3'-tetramethylindocarbocyanine methanesulfonate labeled) colon sensory neurons dissociated from adult S1 dorsal root ganglia. Channels were found in 30% (7/23) of patches in a cell-attached c...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.84.2.836

    authors: Su X,Wachtel RE,Gebhart GF

    更新日期:2000-08-01 00:00:00

  • Full weight-bearing hindlimb standing following stand training in the adult spinal cat.

    abstract::Behavioral and physiological characteristics of standing were studied in nontrained spinal cats and in spinal cats that received daily stand training of the hindlimbs for 12 wk. Training consisted of assisting the cats to stand with full weight support either on both hindlimbs or on one hindlimb (30 min/day, 5 days/wk...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1998.80.1.83

    authors: De Leon RD,Hodgson JA,Roy RR,Edgerton VR

    更新日期:1998-07-01 00:00:00

  • AMPA receptor-mediated miniature synaptic calcium transients in GluR2 null mice.

    abstract::AMPA-type glutamate receptors are normally Ca(2+) impermeable due to the expression of the GluR2 receptor subunit. By using GluR2 null mice we were able to detect miniature synaptic Ca(2+) transients (MSCTs) associated with AMPA-type receptor-mediated miniature synaptic currents at single synapses in primary cortical ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2002.88.1.29

    authors: Wang S,Jia Z,Roder J,Murphy TH

    更新日期:2002-07-01 00:00:00

  • Combined macro-/mesoporous microelectrode arrays for low-noise extracellular recording of neural networks.

    abstract::Microelectrode arrays (MEAs) are appealing tools to probe large neural ensembles and build neural prostheses. Microelectronics microfabrication technologies now allow building high-density MEAs containing several hundreds of microelectrodes. However, several major problems become limiting factors when the size of the ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00711.2011

    authors: Heim M,Rousseau L,Reculusa S,Urbanova V,Mazzocco C,Joucla S,Bouffier L,Vytras K,Bartlett P,Kuhn A,Yvert B

    更新日期:2012-09-01 00:00:00

  • Functional alterations of cat abducens neurons after peripheral tetanus neurotoxin injection.

    abstract::Tetanus neurotoxin (TeNT) cleaves synaptobrevin, a protein involved in synaptic vesicle docking and fusion, thereby preventing neurotransmitter release and causing a functional deafferentation. We injected TeNT into the lateral rectus muscle of adult cats at 0.5 or 5 ng/kg (low and high dose, respectively). In the per...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01006.2002

    authors: Gonzalez-Forero D,de la Cruz RR,Delgado-Garcia JM,Alvarez FJ,Pastor AM

    更新日期:2003-04-01 00:00:00

  • Eye-head coordination in cats.

    abstract::Gaze is the position of the visual axis in space and is the sum of the eye movement relative to the head plus head movement relative to space. In monkeys, a gaze shift is programmed with a single saccade that will, by itself, take the eye to a target, irrespective of whether the head moves. If the head turns simultane...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1984.52.6.1030

    authors: Guitton D,Douglas RM,Volle M

    更新日期:1984-12-01 00:00:00

  • Response properties of neurons in primary somatosensory cortex of owl monkeys reflect widespread spatiotemporal integration.

    abstract::Receptive fields of neurons in somatosensory area 3b of monkeys are typically described as restricted to part of a single digit or palm pad. However, such neurons are likely involved in integrating stimulus information from across the hand. To evaluate this possibility, we recorded from area 3b neurons in anesthetized...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00709.2009

    authors: Reed JL,Qi HX,Zhou Z,Bernard MR,Burish MJ,Bonds AB,Kaas JH

    更新日期:2010-04-01 00:00:00

  • β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP.

    abstract::The widespread noradrenergic innervation in the brain promotes arousal and learning by molecular mechanisms that remain largely undefined. Recent work shows that the β(2)-adrenergic receptor (β(2)AR) is linked to the AMPA-type glutamate receptor subunit GluA1 via stargazin and PSD-95 (Joiner ML, Lise MF, Yuen EY, Kam ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00374.2011

    authors: Qian H,Matt L,Zhang M,Nguyen M,Patriarchi T,Koval OM,Anderson ME,He K,Lee HK,Hell JW

    更新日期:2012-05-01 00:00:00

  • Glutamate-gated chloride channel with glutamate-transporter-like properties in cone photoreceptors of the tiger salamander.

    abstract::1. Using the patch-clamp technique, we investigated whether the glutamate-elicited current in mechanically isolated cone photoreceptors from the salamander retina is generated by a Cl- channel or a glutamate transporter. 2. The current reversed near the equilibrium potential for Cl-, was decreased by three Cl- channel...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1995.74.4.1760

    authors: Picaud SA,Larsson HP,Grant GB,Lecar H,Werblin FS

    更新日期:1995-10-01 00:00:00

  • Relationships between neck muscle electromyography and three-dimensional head kinematics during centrally induced torsional head perturbations.

    abstract::The relationship between neck muscle electromyography (EMG) and torsional head rotation (about the nasooccipital axis) is difficult to assess during normal gaze behaviors with the head upright. Here, we induced acute head tilts similar to cervical dystonia (torticollis) in two monkeys by electrically stimulating 20 in...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00312.2012

    authors: Farshadmanesh F,Byrne P,Wang H,Corneil BD,Crawford JD

    更新日期:2012-12-01 00:00:00

  • Responses of reticulospinal neurons in intact lamprey to vestibular and visual inputs.

    abstract::A lamprey maintains the dorsal-side-up orientation due to the activity of postural control system driven by vestibular input. Visual input can affect the body orientation: illumination of one eye evokes ipsilateral roll tilt. An important element of the postural network is the reticulospinal (RS) neurons transmitting ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.83.2.864

    authors: Deliagina TG,Fagerstedt P

    更新日期:2000-02-01 00:00:00

  • Neural timing signal for precise tactile timing judgments.

    abstract::The brain can precisely encode the temporal relationship between tactile inputs. While behavioural studies have demonstrated precise interfinger temporal judgments, the underlying neural mechanism remains unknown. Computationally, two kinds of neural responses can act as the information source. One is the phase-locked...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00790.2015

    authors: Kuroki S,Watanabe J,Nishida S

    更新日期:2016-03-01 00:00:00

  • Permeation properties of the hair cell mechanotransducer channel provide insight into its molecular structure.

    abstract::Mechanoelectric transducer (MET) channels, located near stereocilia tips, are opened by deflecting the hair bundle of sensory hair cells. Defects in this process result in deafness. Despite this critical function, the molecular identity of MET channels remains a mystery. Inherent channel properties, particularly those...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01178.2011

    authors: Pan B,Waguespack J,Schnee ME,LeBlanc C,Ricci AJ

    更新日期:2012-05-01 00:00:00

  • Preferential representation of instructed target location versus limb trajectory in dorsal premotor area.

    abstract::The dorsal premotor area (PMd) of monkeys has been implicated in processes relating to movement preparation and movement selection. In the present study, we sought to determine whether PMd neurons that are activated during a delayed reaching task have directional responses that reflect either the target (i.e., the goa...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.77.3.1195

    authors: Shen L,Alexander GE

    更新日期:1997-03-01 00:00:00

  • Propagation of spindle waves in a thalamic slice model.

    abstract::1. We study the propagation and dynamics of spindle waves in thalamic slices by developing and analyzing a model of reciprocally coupled populations of excitatory thalamocortical (TC) neurons and inhibitory thalamic reticular (RE) neurons. 2. Each TC neuron has three intrinsic ionic currents: a low-threshold T-type Ca...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.75.2.750

    authors: Golomb D,Wang XJ,Rinzel J

    更新日期:1996-02-01 00:00:00

  • Tissue resistance changes and the profile of synchronized neuronal activity during ictal events in the low-calcium model of epilepsy.

    abstract::Population spikes vary in size during prolonged epileptic ("ictal") discharges, indicating variations in neuronal synchronization. Here we investigate the role of changes in tissue electrical resistivity in this process. We used the rat hippocampal slice, low-Ca(2+) model of epilepsy and measured changes in pyramidal ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00123.2004

    authors: Fox JE,Bikson M,Jefferys JG

    更新日期:2004-07-01 00:00:00

  • Modulation of muscle responses evoked by transcranial magnetic stimulation during the acquisition of new fine motor skills.

    abstract::1. We used transcranial magnetic stimulation (TMS) to study the role of plastic changes of the human motor system in the acquisition of new fine motor skills. We mapped the cortical motor areas targeting the contralateral long finger flexor and extensor muscles in subjects learning a one-handed, five-finger exercise o...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1995.74.3.1037

    authors: Pascual-Leone A,Nguyet D,Cohen LG,Brasil-Neto JP,Cammarota A,Hallett M

    更新日期:1995-09-01 00:00:00