Abstract：
:To design hybrid tumour necrosis factor-alpha (TNF-alpha) applicable to systemic anti-tumour therapeutic use, we assessed the relationships among the molecular size of hybrid TNF-alpha, in vitro bioactivity and in vivo anti-tumour potency. Natural human TNF-alpha was covalently modified with polyethylene glycol (PEG) of various number-average molecular weights (Mn = 2000, 5000, 12,000). The in vitro bioactivity of PEG-modified TNF-alpha s decreased with an increase in the degree of PEG modification, irrespective of the molecular weight of PEG. This decrease in the specific bioactivity markedly increased with an increase in the molecular weight of the attached PEG. The in vivo anti-tumour effects of the hybrid TNF-alpha s with a molecular size from 100 to 110 kDa, which had more than 50% of specific bioactivity of native TNF-alpha, were significantly superior to other PEG-TNF-alpha s. These hybrid TNF-alpha s showed over ten times greater anti-tumour effects than native TNF-alpha. Thus, the molecular size, which was determined by the degree of PEG modification and PEG molecular weight, influences the specific activity and anti-tumour effects of hybrid TNF-alpha.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Tsutsumi Y,Tsunoda S,Kamada H,Kihira T,Nakagawa S,Kaneda Y,Kanamori T,Mayumi Tdoi
10.1038/bjc.1996.495subject
Has Abstractpub_date
1996-10-01 00:00:00pages
1090-5issue
7eissn
0007-0920issn
1532-1827journal_volume
74pub_type
杂志文章abstract：:Eleven human bladder carcinomata of different degrees of differentiation were implanted in mice immunosuppressed by thymectomy, anti-thymocyte serum and x-rays. Seven carcinomata grew well and one poorly and 3 produced mainly fibrous nodules in the mice. Normal human bladder tissues were grown from 4 other patients. T...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1975.30
更新日期：1975-02-01 00:00:00
abstract：:Elevated expression of matrix metalloproteinases (MMPs), a family of secreted proteinases that degrade matrix components of basement membranes and connective tissues, is strongly correlated with malignant expression in various human epithelial cancers and epithelial cancer cell lines. We have tested whether elevated l...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.119
更新日期：1998-03-01 00:00:00
abstract：:We examined polymorphisms in exons 3 and 4 of microsomal epoxide hydrolase in 101 patients with colon cancer and compared the results with 203 control samples. The frequency of the exon 3 T to C mutation was higher in cancer patients than in controls (odds ratio 3.8; 95% confidence intervals 1.8-8.0). This sequence al...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690028
更新日期：1999-01-01 00:00:00
abstract：:T-lymphocyte maturation associated protein, MAL, has been described as a tumour-suppressor gene with diagnostic value in colorectal and oesophageal cancers, and can be inactivated by promoter hypermethylation. The aim of this study was to analyse the prevalence of MAL promoter hypermethylation and the association with...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604777
更新日期：2008-12-02 00:00:00
abstract：:The expression of urokinase-type plasminogen activator (uPA) was investigated in squamous cell carcinoma of the human larynx. For this purpose, tissue extracts from 25 matched samples of normal mucosa and neoplastic larynx were compared for the levels of uPA activity as evaluated by a chromogenic PA assay and sodium d...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.512
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND:Pancreatic stellate cells (PSCs, which produce the stroma of pancreatic cancer (PC)) interact with cancer cells to facilitate PC growth. A candidate growth factor pathway that may mediate this interaction is the HGF-c-MET pathway. METHODS:Effects of HGF inhibition (using a neutralising antibody AMG102) alon...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2015.478
更新日期：2016-02-02 00:00:00
abstract：BACKGROUND:CD26 is a multifunctional membrane-bound glycoprotein that regulates tumour growth in addition to its other activities. Because disease aggressiveness is correlated with CD26 expression in several T-cell malignancies, we decided to investigate the invasiveness of cells expressing different levels of CD26. M...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605236
更新日期：2009-09-15 00:00:00
abstract：BACKGROUND:Lapatinib, a dual EGFR and HER2 inhibitor has shown disappointing results in clinical trials of metastatic oesophago-gastric adenocarcinomas (OGAs), and in vitro studies suggest that MET, IGFR, and HER3 confer resistance. This trial applied Lapatinib in the curative neoadjuvant setting and investigated the f...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/bjc.2015.342
更新日期：2015-11-03 00:00:00
abstract：:Cancer incidence in the Falkland Islands, 1989-2000, was compared with rates in England and Wales, from which most Islanders originate. Colon and rectum cancer incidence was significantly raised 1989-93 but greatly reduced after 1994, when colonoscopic screening in high-risk families and sigmoidoscopic screening in th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2001.2099
更新日期：2001-11-02 00:00:00
abstract：:We have recently reported the activation of a new oncogene in human papillary thyroid carcinomas. This oncogene, named PTC, is a novel rearranged version of the ret proto-oncogene. In fact PTC is the product of the fusion of the tyrosine kinase domain of the ret proto-oncogene with the 5'-terminal region of another ge...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1992.416
更新日期：1992-12-01 00:00:00
abstract：:A standardised, effective systemic therapy for metastatic neuroendocrine tumours (NETs) has not been established to date. We reviewed the management of 15 patients with inoperable, metastatic NET treated systematically with a combination chemotherapy regimen of infusional 5-fluorouracil, folinic acid and streptozocin....
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601167
更新日期：2003-08-04 00:00:00
abstract：:One hundred and seventy-eight patients with non metastatic inflammatory breast cancer (IBC) have been treated at the Centre H. Becquerel. Median follow up is 67 months (6-178). Every patient received neoadjuvant chemotherapy (mean number of cycles = 4; range: 2-8), followed by a loco regional treatment (radiotherapy =...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.109
更新日期：1993-03-01 00:00:00
abstract：BACKGROUND:The mitogen-activated protein kinase (MAPK) pathway has been implicated in the molecular pathogenesis of human cancers, including metastatic colorectal cancer (mCRC). This provides a rationale for the development of MAPK-targeted agents such as pimasertib. METHODS:Patients with KRAS mutant mCRC were treated...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/bjc.2015.144
更新日期：2015-06-09 00:00:00
abstract：PURPOSE:To determine the feasibility of induction chemotherapy and chemo-IMRT in head and neck squamous cell cancers at risk of bilateral nodal spread (midline tumours) and to evaluate whether bilateral superficial lobe parotid-sparing IMRT can reduce the incidence of ⩾G2 subjective xerostomia. METHODS:Patients with m...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.553
更新日期：2015-01-06 00:00:00
abstract：:We investigated trends in first-time hospital admissions and deaths attributable to hepatocellular carcinoma (HCC) in a large population-based cohort of 22 073 individuals diagnosed with hepatitis C viral (HCV) infection through laboratory testing in Scotland in 1991-2006. We identified new cases of HCC through record...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604563
更新日期：2008-09-02 00:00:00
abstract：:Sodium polyanethol sulphonate (SPS) when incorporated into rat erythrocyte lysate (REL) containing semi-solid agar medium at 1 mg ml-1. markedly enhanced colony formation by a number of anchorage-independent cell lines. REL usually needed to be included for the expression of SPS induced potentiation as in its absence ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1984.230
更新日期：1984-11-01 00:00:00
abstract：:Both misonidazole (MISO) and hyperthermia are known to enhance the radiation response of hypoxic cells, and to be selectively cytotoxic against cells in a hypoxic and acidic environment. The ability of these conditions to modify the effect of irradiation and their individual relationship was studied in a C3H mammary c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1980.2
更新日期：1980-01-01 00:00:00
abstract：BACKGROUND:Clear cell renal cancer frequently harbours von Hippel-Lindau (VHL) gene mutations, leading to stabilisation of the hypoxia-inducible factors (HIFs) and expression of their target genes. We investigated HIF-1 and HIF-2 in the regulation of microRNA-210 (miR-210), and its clinical relevance in renal tumours. ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.56
更新日期：2013-03-19 00:00:00
abstract：:Serum samples taken between 6 and 20 weeks of gestation were obtained from 28 mothers who gave birth to cryptorchid sons (cases) and from 108 control mothers. In comparison with controls the cases had 10% higher geometric mean oestradiol (95% CI -13% to +39%: P=0.42) and 10% lower geometric mean testosterone (95% CI -...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.121
更新日期：1996-03-01 00:00:00
abstract：:The effect of the synthetic progesterone, megestrol acetate, on weight loss induced by both tumour necrosis factor alpha (TNF) as a model for the cachexia accompanying the acquired immunodeficiency syndrome and by a cachexia-inducing tumour (MAC16) has been studied in NMRI mice. Megestrol acetate was effective in prev...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1990.310
更新日期：1990-09-01 00:00:00
abstract：:The aim of this study was to assess the relationship between haemoglobin level and quality-of-life in anaemic cancer patients. Patients, diagnosed with one of four cancers, were recruited if their haemoglobin level was <12 g dl(-1) (female) or <13 g dl(-1) (male). The condition-specific Functional Assessment of Cancer...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/sj.bjc.6600247
更新日期：2002-04-22 00:00:00
abstract：:Male inbred Fischer rats were fed phenobarbitone-Na at a level of 500 parts/10(6) in the diet for 1 week, followed by 1000 parts 10(6) for 103 weeks at which time the survivors were killed. Thirty-three treated rats survived to 80 weeks. Before 80 weeks, no animals showed hyperplastic lesions. Of the 33 rats surviving...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.62
更新日期：1978-03-01 00:00:00
abstract：:Recent investigations have increasingly focussed attention on the roles of intracellular vesicle trafficking in the regulation of epithelial polarity and transformation. Rab25, an epithelial-specific member of the Rab family of small GTPases, has been associated with several epithelial cancers. Whereas Rab25 overexpre...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/sj.bjc.6605983
更新日期：2011-01-04 00:00:00
abstract：:Interleukin 6 (IL-6) and its soluble receptor (IL-6sR) can markedly stimulate aromatase activity in cultured fibroblasts derived from normal or malignant breast tissues. IL-6 acts by binding to a low-affinity membrane-spanning receptor (IL-6R), which must associate with a high-affinity receptor (gp130) for signal tran...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600785
更新日期：2003-02-24 00:00:00
abstract：:Little is known about the factors that enable the mobilisation of human mesenchymal stem cells (MSC) from the bone marrow into the blood stream and their recruitment to and retention in the tumour. We found specific migration of MSC towards growth factors present in pancreatic tumours, such as PDGF, EGF, VEGF and spec...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604508
更新日期：2008-08-19 00:00:00
abstract：:Three hundred and ninety women participated in the quality of life (QL) study of ACCOG1, a high-dose vs conventional adjuvant chemotherapy breast cancer trial, for patients with a high risk of relapse. Patients completed the European Organisation for Research and Treatment of Cancer QLQ-C30, questions on menopausal sy...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603454
更新日期：2006-12-18 00:00:00
abstract：:BALB/c mice born to and nursed by females immunized against MSV-M showed a reduced tumour incidence and a high tumour regression rate following MSV-M injection at 7-14 days of age. Females immunized long before mating could also confer protection to their offspring whereas females immunized after parturition could not...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1973.143
更新日期：1973-09-01 00:00:00
abstract：:Deregulated expression of myc proto-oncogenes is implicated in several human neoplasias. We analysed the expression of c-myc, N-myc, L-myc, max and RB1 mRNAs in a panel of human gliomas and glioma cell lines and compared the findings with normal neural cells. The max and RB1 genes were included in the study because th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.3
更新日期：1994-01-01 00:00:00
abstract：:Clear cell sarcoma of tendons and aponeuroses (malignant melanoma of soft parts) and conventional malignant melanoma may demonstrate significant morphologic overlap at the light microscopic and ultrastructural level. Consequently, the clinically relevant distinction between primary clear cell sarcoma and metastatic me...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1628
更新日期：2001-02-01 00:00:00
abstract：:We evaluated the myelotoxicity and the anti-tumor potential of tallimustine, three of its analogues and carzelesin, with melphalan as reference substance. Tallimustine was tested by clonogenic assays on both human bone marrow (BM) and cord blood (hCB) cells, the other compounds on hCB only. The degree of inhibition of...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.155
更新日期：1997-01-01 00:00:00
