Enterotoxin production by coagulase-negative staphylococci isolated from goats' milk and cheese.

Abstract:

:An antigen related to the Enterotoxin E from Staphylococcus aureus was produced by ten of 187 coagulase-negative staphylococci (CNS) isolated from goats' milk, whey and cheese in quantities ranging from 10 to 90 ng/ml supernatant. The enterotoxin-producing strains were identified at the species level as S. simulans, S. xylosus, S. equorum, S. lentus and S. capitis. Detection of the enterotoxins was done by the VIDAS SET test (bioMérieux) and by an indirect double-sandwich ELISA technique using anti-enterotoxin monoclonal antibodies. The results obtained were further confirmed by Southern blotting, using two radioactive oligonucleotide probes that hybridized specifically with the gene of S. aureus coding for the enterotoxin E.

journal_name

Int J Food Microbiol

authors

Vernozy-Rozand C,Mazuy C,Prevost G,Lapeyre C,Bes M,Brun Y,Fleurette J

doi

10.1016/0168-1605(96)00952-x

subject

Has Abstract

pub_date

1996-07-01 00:00:00

pages

271-80

issue

3

eissn

0168-1605

issn

1879-3460

pii

016816059600952X

journal_volume

30

pub_type

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