Abstract:
:We report a sensitive method for the estimation of quinine (Qn), cinchonine (Cn), and cinchonidine (Cnd) and a new method based on fluorescence enhancement and detection and quantification of quinidine (Qnd) from Cinchona stem bark and its formulations, using HPTLC. Standard solutions of Qn, Qnd, Cn, and Cnd were applied on precoated HPTLC plates and developed with chloroform/diethylamine (9.6:1.4 v/v). The plates were scanned and quantified at 226 nm for Qn, Cn, Cnd and for Qnd at 366 nm in fluorescence and reflectance mode ([symbol: see text] K400 filter). The method was validated for precision, accuracy and repeatability. Further, the stem bark of Cinchona officinalis and some herbal and homeopathic formulations were evaluated for their individual alkaloid content applying the developed method.
journal_name
Planta Medjournal_title
Planta medicaauthors
Ravishankara MN,Shrivastava N,Padh H,Rajani Mdoi
10.1055/s-2001-11995subject
Has Abstractpub_date
2001-04-01 00:00:00pages
294-6issue
3eissn
0032-0943issn
1439-0221journal_volume
67pub_type
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