HPTLC method for the estimation of alkaloids of Cinchona officinalis stem bark and its marketed formulations.

Abstract:

:We report a sensitive method for the estimation of quinine (Qn), cinchonine (Cn), and cinchonidine (Cnd) and a new method based on fluorescence enhancement and detection and quantification of quinidine (Qnd) from Cinchona stem bark and its formulations, using HPTLC. Standard solutions of Qn, Qnd, Cn, and Cnd were applied on precoated HPTLC plates and developed with chloroform/diethylamine (9.6:1.4 v/v). The plates were scanned and quantified at 226 nm for Qn, Cn, Cnd and for Qnd at 366 nm in fluorescence and reflectance mode ([symbol: see text] K400 filter). The method was validated for precision, accuracy and repeatability. Further, the stem bark of Cinchona officinalis and some herbal and homeopathic formulations were evaluated for their individual alkaloid content applying the developed method.

journal_name

Planta Med

journal_title

Planta medica

authors

Ravishankara MN,Shrivastava N,Padh H,Rajani M

doi

10.1055/s-2001-11995

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

294-6

issue

3

eissn

0032-0943

issn

1439-0221

journal_volume

67

pub_type

信件
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