Hyperpolarization-activated ion currents in cultured rat cortical and spinal cord astrocytes.

Abstract:

:Hyperpolarization-activated currents were recorded from rat brain cortical and spinal cord astrocytes maintained in culture. Spinal cord astrocytes expressed primarily an inward rectifier potassium current characterized by time-dependent inactivation, a strong dependence on extracellular Na+ and insensitivity to intracellular GTP-gamma-S (0.2 mM). In cortical astrocytes voltage clamp protocols aimed to elicit currents activated at, or negative to cell membrane potentials led to the development of two distinct ion currents. The most prominent current resembled the inward rectifier potassium current. This component was sensitive to blockade by extracellular cesium and was greatly reduced during recordings performed with GTP-gamma-S (0.2 Mm) added to the pipette solutions. The remaining current component was similar to the endothelial I ha current. I ha conductance was enhanced by extracellular potassium and the current reversal potential behaved as expected for a mixed cation, Na+/K+ current. I ha was nearly abolished after removal of extracellular Na. These results are consistent with the expression of a novel mixed cation conductance in glial cells, possibly involved in extracellular potassium buffering.

journal_name

Glia

journal_title

Glia

authors

Guatteo E,Stanness KA,Janigro D

doi

10.1002/(SICI)1098-1136(199603)16:3<196::AID-GLIA2

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

196-209

issue

3

eissn

0894-1491

issn

1098-1136

pii

10.1002/(SICI)1098-1136(199603)16:3<196::AID-GLIA2

journal_volume

16

pub_type

杂志文章

相关文献

GLIA文献大全
  • Neuronal dependency of the glycine transporter GLYT1 expression in glial cells.

    abstract::Two membrane-localized transporter proteins (GLYT1 and GLYT2) are responsible for removal of extracellular glycine in the mammalian CNS. Whereas GLYT1 seems to be expressed mainly in glial cells, GLYT2 is neuronal. The highest concentrations of both transporters are found in glycinergic areas of the nervous system. Th...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199706)20:2<155::aid-glia7

    authors: Zafra F,Poyatos I,Gimenez C

    更新日期:1997-06-01 00:00:00

  • Vimentin immunoreactive glial cells in the fish optic nerve: implications for regeneration.

    abstract::The poor regenerative ability of neurons of the central nervous system in mammals, as compared with their counterpart in fish or amphibians, is thought to stem from differences in their immediate nonneuronal environment and its response to axonal injury. We describe one aspect of the environmental response to axonal i...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440100104

    authors: Cohen I,Sivron T,Lavie V,Blaugrund E,Schwartz M

    更新日期:1994-01-01 00:00:00

  • Neuregulins and erbB receptor expression in adult human oligodendrocytes.

    abstract::We have previously reported that neonatal rat oligodendrocytes (OLGs) express and secrete neuregulins (NRGs) (Raabe et al., 1997). This laboratory has also shown that NRGs stimulate the differentiation of neonatal rat OLGs and that these cells express the erbB receptors for NRGs (Raabe et al., 1997). In this study, we...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Deadwyler GD,Pouly S,Antel JP,Devries GH

    更新日期:2000-12-01 00:00:00

  • Transient hypoxia/hypoglycemia upregulates endothelin B receptors in cultured rat astrocytes.

    abstract::Endothelins are potent vasoactive peptides that bind to their specific receptors, playing an important role in the CNS under physiological and pathophysiological conditions. Astrocytes, which have been shown to express these receptors, also have a considerable role to play under physiological and pathophysiological co...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Shibaguchi H,Himeno A,Shigematsu K,Kataoka Y,Niwa M

    更新日期:2000-07-01 00:00:00

  • Hypoglycemia, brain energetics, and hypoglycemic neuronal death.

    abstract::Hypoglycemia is a common and serious problem among diabetic patients receiving treatment with insulin or other glucose-lowering drugs. Moderate hypoglycemia impairs neurological function, and severe hypoglycemia leads to death of selectively vulnerable neurons. Recent advances have shed new light on the underlying pro...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.20440

    authors: Suh SW,Hamby AM,Swanson RA

    更新日期:2007-09-01 00:00:00

  • A functional role of NMDA receptor in regulating the differentiation of oligodendrocyte precursor cells and remyelination.

    abstract::Differentiation of oligodendrocyte precursor cells (OPCs) is the most important event for the myelination of central nervous system (CNS) axons during development and remyelination in demyelinating diseases, while the underlying molecular mechanisms remain largely unknown. Here we show that NMDA receptor (NMDAR) is a ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22469

    authors: Li C,Xiao L,Liu X,Yang W,Shen W,Hu C,Yang G,He C

    更新日期:2013-05-01 00:00:00

  • Astroglia contribute to the pathogenesis of spinocerebellar ataxia Type 1 (SCA1) in a biphasic, stage-of-disease specific manner.

    abstract::Spinocerebellar ataxia type 1 (SCA1) is a fatal, dominantly inherited neurodegenerative disease caused by the expansion of CAG repeats in the Ataxin-1 (ATXN1) gene. SCA1 is characterized by balance and coordination deficits due to the predominant loss of Purkinje neurons in the cerebellum. We previously demonstrated t...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23451

    authors: Kim JH,Lukowicz A,Qu W,Johnson A,Cvetanovic M

    更新日期:2018-09-01 00:00:00

  • Membrane-initiated effects of progesterone on calcium dependent signaling and activation of VEGF gene expression in retinal glial cells.

    abstract::Neurosteroids, such as progesterone, influence central nervous system development and function by regulating a broad spectrum of physiological processes. Here, we investigated membrane-initiated actions of progesterone in the retina and identified the membrane-associated progesterone receptor component 1 (PGRMC1). We ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20523

    authors: Swiatek-De Lange M,Stampfl A,Hauck SM,Zischka H,Gloeckner CJ,Deeg CA,Ueffing M

    更新日期:2007-08-01 00:00:00

  • NADPH oxidase and aging drive microglial activation, oxidative stress, and dopaminergic neurodegeneration following systemic LPS administration.

    abstract::Parkinson's disease is characterized by a progressive degeneration of substantia nigra (SN) dopaminergic neurons with age. We previously found that a single systemic lipopolysaccharide (LPS, 5 mg/kg, i.p.) injection caused a slow progressive loss of tyrosine hydroxylase immunoreactive (TH+IR) neurons in SN associated ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22479

    authors: Qin L,Liu Y,Hong JS,Crews FT

    更新日期:2013-06-01 00:00:00

  • Signaling proteins in the axoglial apparatus of sciatic nerve nodes of Ranvier.

    abstract::During action potential conduction, the axonal specializations at the node, together with the adjacent paranodal terminations of the myelin sheath, interact with glial processes that invest the nodal gap. The nature of the mutual signals between axons and myelinating glia, however, are not well understood. Here we hav...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20448

    authors: Toews JC,Schram V,Weerth SH,Mignery GA,Russell JT

    更新日期:2007-01-15 00:00:00

  • Inhibitory effect of minocycline on amyloid beta fibril formation and human microglial activation.

    abstract::Minocycline, a derivative of the antibiotic tetracycline, displays neuroprotective properties in various models of neurodegenerative diseases and is now used in clinical trials, because of its relative safety and tolerability. Minocycline passes the blood-brain barrier and is presumed to inhibit microglial activation....

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20268

    authors: Familian A,Boshuizen RS,Eikelenboom P,Veerhuis R

    更新日期:2006-02-01 00:00:00

  • In vivo silencing of miR-125a-3p promotes myelin repair in models of white matter demyelination.

    abstract::In the last decade, microRNAs have been increasingly recognized as key modulators of glial development. Recently, we identified miR-125a-3p as a new player in oligodendrocyte physiology, regulating in vitro differentiation of oligodendrocyte precursor cells (OPCs). Here, we show that miR-125a-3p is upregulated in acti...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23819

    authors: Marangon D,Boda E,Parolisi R,Negri C,Giorgi C,Montarolo F,Perga S,Bertolotto A,Buffo A,Abbracchio MP,Lecca D

    更新日期:2020-10-01 00:00:00

  • LPS-induced expression of a novel chemokine receptor (L-CCR) in mouse glial cells in vitro and in vivo.

    abstract::There is increasing evidence that chemokines, specialized regulators of the peripheral immune system, are also involved in the physiology and pathology of the CNS. It is known that glial cells (astrocytes and microglia) express various chemokine receptors like CCR1, -3, -5, and CXCR4. We have investigated the possible...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10156

    authors: Zuurman MW,Heeroma J,Brouwer N,Boddeke HW,Biber K

    更新日期:2003-03-01 00:00:00

  • Astrocytic poly(ADP-ribose) polymerase-1 activation leads to bioenergetic depletion and inhibition of glutamate uptake capacity.

    abstract::Poly(ADP-ribose) polymerase-1 (PARP-1) is a ubiquitous nuclear enzyme involved in genomic stability. Excessive oxidative DNA strand breaks lead to PARP-1-induced depletion of cellular NAD(+), glycolytic rate, ATP levels, and eventual cell death. Glutamate neurotransmission is tightly controlled by ATP-dependent astroc...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20936

    authors: Tang KS,Suh SW,Alano CC,Shao Z,Hunt WT,Swanson RA,Anderson CM

    更新日期:2010-03-01 00:00:00

  • High-yield isolation of murine microglia by mild trypsinization.

    abstract::Microglia can be isolated with high purity but low yield by shaking off loosely adherent cells from mixed glial cultures. Here we describe a new technique for isolating microglia with an average yield close to 2,000,000 microglial cells/mouse pup, more than five times higher than that of the shaking method. Confluent ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10274

    authors: Saura J,Tusell JM,Serratosa J

    更新日期:2003-12-01 00:00:00

  • MiR-409-3p and MiR-1896 co-operatively participate in IL-17-induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling.

    abstract::Th17 cells and interleukin-17 (IL-17) have been found to play an important role in the pathology of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Response to IL-17, reactive astrocytes accompany with immune cells infiltration and axonal damage in MS/EAE. However, the ro...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23530

    authors: Liu X,Zhou F,Yang Y,Wang W,Niu L,Zuo D,Li X,Hua H,Zhang B,Kou Y,Guo J,Kong F,Pan W,Gao D,Meves JM,Sun H,Xue M,Zhang Q,Wang Y,Tang R

    更新日期:2019-01-01 00:00:00

  • Oligodendrocyte precursor cells generate pituicytes in vivo during neurohypophysis development.

    abstract::In the vertebrate brain, much remains to be understood concerning the origin of glial cell diversity and the potential lineage relationships between the various types of glia. Besides astrocytes and myelin-forming oligodendrocytes, other macroglial cell populations are found in discrete areas of the central nervous sy...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20282

    authors: Virard I,Coquillat D,Bancila M,Kaing S,Durbec P

    更新日期:2006-02-01 00:00:00

  • Interactions between TLR7 and TLR9 agonists and receptors regulate innate immune responses by astrocytes and microglia.

    abstract::Toll-like receptors 7 (TLR7) and 9 (TLR9) are important mediators of innate immune responses. Both receptors are located in endosomal compartments, recognize nucleic acids, and signal via Myeloid differentiation factor 88 (MyD88). In the current study, we analyzed TLR7 and TLR9 induced activation of astrocytes and mic...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20952

    authors: Butchi NB,Du M,Peterson KE

    更新日期:2010-04-15 00:00:00

  • FGFs stimulate IGF binding protein synthesis without affecting IGF synthesis in rat astroblasts in primary culture.

    abstract::The production of insulin-like growth factor (IGF)-I and -II and their binding proteins (BPs) has been studied in new-born rat astroblasts at confluency in primary culture. Under the influence of fibroblast growth factors (FGFs) (acidic and basic), the morphology of the astroblasts was altered, 125I-deoxyuridine incor...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440040405

    authors: Loret C,Janet T,Labourdette G,Schneid H,Binoux M

    更新日期:1991-01-01 00:00:00

  • Histamine and prostanoid receptors on glial cells.

    abstract::Glial cells in vitro express at least two types (H1 and H2) of histamine receptors and three types (EP, FP, and TP) of prostanoid receptors. The receptors expressed by glial cells differ according to the cell type and source in the brain. Furthermore primary astrocytes of same type derived from the same brain region a...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.440110205

    authors: Inagaki N,Wada H

    更新日期:1994-06-01 00:00:00

  • Novel microtubule-associated protein-2 isoform is expressed early in human oligodendrocyte maturation.

    abstract::We have identified a developmentally regulated, oligodendrocyte-specific protein, designated microtubule-associated protein-2 expressing exon 13 (MAP-2+13), in the human central nervous system (CNS). Monoclonal antibodies directed against MAP-2+13 labeled oligodendrocytes in the white matter of human fetal spinal cord...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Shafit-Zagardo B,Davies P,Rockwood J,Kress Y,Lee SC

    更新日期:2000-02-01 00:00:00

  • Natural and induced cytotoxicity of oligodendrocytes by microglia is inhibitable by TGF beta.

    abstract::Blood macrophages and brain macrophages (microglia) have been implicated in demyelination and destruction of the oligodendrocyte in multiple sclerosis (MS), a disease affecting primarily white matter of the central nervous system (CNS). In this study, we demonstrate that at high effector to target cell ratios, normal ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440040311

    authors: Merrill JE,Zimmerman RP

    更新日期:1991-01-01 00:00:00

  • Sub-micromolar increase in [Ca(2+)](i) triggers delayed exocytosis of ATP in cultured astrocytes.

    abstract::Astrocytes release a variety of transmitter molecules, which mediate communication between glial cells in the brain and modulate synaptic transmission. ATP is a major glia-derived transmitter, but the mechanisms and kinetics of ATP release from astrocytes remain largely unknown. Here, we combined epifluorescence and t...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20590

    authors: Pryazhnikov E,Khiroug L

    更新日期:2008-01-01 00:00:00

  • Fish optic nerve oligodendrocytes support axonal regeneration of fish and mammalian retinal ganglion cells.

    abstract::Segments from adult fish and rat retinae were explanted on myelin-marker expressing oligodendrocytes derived from the regenerating goldfish optic nerve. Fish axons grew in high density and even rat retinal axons regenerated to considerable length on the surface of the fish oligodendrocytes, suggesting that this type o...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440080102

    authors: Bastmeyer M,Bähr M,Stuermer CA

    更新日期:1993-05-01 00:00:00

  • Dedifferentiated Schwann cells secrete progranulin that enhances the survival and axon growth of motor neurons.

    abstract::Schwann cells (SCs), the primary glia in the peripheral nervous system (PNS), display remarkable plasticity in that fully mature SCs undergo dedifferentiation and convert to repair SCs upon nerve injury. Dedifferentiated SCs provide essential support for PNS regeneration by producing signals that enhance the survival ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23547

    authors: Hyung S,Im SK,Lee BY,Shin J,Park JC,Lee C,Suh JF,Hur EM

    更新日期:2019-02-01 00:00:00

  • E587 antigen is upregulated by goldfish oligodendrocytes after optic nerve lesion and supports retinal axon regeneration.

    abstract::The properties of glial cells in lesioned nerves contribute quite substantially to success or failure of axon regeneration in the CNS. Goldfish retinal axons regenerate after optic nerve lesion (ONS) and express the L1-like cell adhesion protein E587 antigen on their surfaces. Goldfish oligodendrocytes in vitro also p...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Ankerhold R,Leppert CA,Bastmeyer M,Stuermer CA

    更新日期:1998-07-01 00:00:00

  • Pure astrocyte cultures derived from cells isolated from mature brain.

    abstract::Enriched preparations of oligodendrocytes, isolated either from adult bovine brain or from 30-day-old rat brain, eventually yield cultures in MEM-15% calf serum that contain, in addition to oligodendrocytes, proliferating astrocytes and variable numbers of fibroblast-like cells. If these cultures are switched to a ser...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440010608

    authors: Norton WT,Farooq M,Chiu FC,Bottenstein JE

    更新日期:1988-01-01 00:00:00

  • ErbB signaling has a role in radial sorting independent of Schwann cell number.

    abstract::In the peripheral nervous system, Schwann cells make myelin, a specialized sheath that is essential for rapid axonal conduction of action potentials. Immature Schwann cells initially interact with many axons, but, through a process termed radial sorting, eventually interact with one segment of a single axon as promyel...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21175

    authors: Raphael AR,Lyons DA,Talbot WS

    更新日期:2011-07-01 00:00:00

  • Anandamide enhances IL-10 production in activated microglia by targeting CB(2) receptors: roles of ERK1/2, JNK, and NF-kappaB.

    abstract::The endocannabinoid system exhibits anti-inflammatory properties by regulating cytokine production. Anandamide (AEA) down-regulates proinflammatory cytokines in a viral model of multiple sclerosis (MS). However, little is known about the mechanisms by which AEA exerts these effects. Microglial cells are the main sourc...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20907

    authors: Correa F,Hernangómez M,Mestre L,Loría F,Spagnolo A,Docagne F,Di Marzo V,Guaza C

    更新日期:2010-01-15 00:00:00

  • TNF-alpha- and TRAIL-resistant glioma cells undergo autophagy-dependent cell death induced by activated microglia.

    abstract::The role of microglia, the brain resident macrophages, in glioma biology is still ill-defined. Despite their cytotoxic potential, these cells that significantly infiltrate the tumor mass seem to support tumor growth rather than tumor eradication. A proper activation of microglia anti-tumor activities within the tumor ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20785

    authors: Mora R,Abschuetz A,Kees T,Dokic I,Joschko N,Kleber S,Geibig R,Mosconi E,Zentgraf H,Martin-Villalba A,Régnier-Vigouroux A

    更新日期:2009-04-01 00:00:00