Abstract:
:A simple and rapid strategy for restriction mapping based on sequence-specific triple-helix affinity capture (TAC) was developed. The strategy was applied to the analysis of cosmid clones by the construction of a new cosmid vector, ScosTriplex-II, containing two different triple-helix-forming sequences flanking the cloning site of the original SuperCos-1 cosmid vector. For restriction mapping, the recombinant cosmid DNA is digested with NotI restriction enzyme or with one of four intron-encoded endonucleases for excision of intact inserts followed by controlled partial digestion with a mapping enzyme used in conjunction with the corresponding methyltransferase. The partial digestion products are combined with biotinylated triple-helix-forming oligonucleotides to form a triple-helical complex. The triple-helix complexes are immobilized on streptavidin-coated magnetic beads, washed, and eluted with pH 9 buffer solution. The fragments are separated and directly sized by agarose gel electrophoresis. Bidirectional maps are obtained simultaneously by binding to the two different triple-helix-forming oligonucleotides. No probe labeling, gel drying, blotting to membranes, hybridization, or autoradiography is necessary. Also, TAC conditions that permit gel-free isolation of the terminal restriction fragments from cosmid inserts were found. These advantages afforded by ScosTriplex-II should facilitate the automation of cosmid restriction site fingerprinting needed for large-scale mapping and sequencing projects.
journal_name
Genomicsjournal_title
Genomicsauthors
Ji H,Francisco T,Smith LM,Guilfoyle RAdoi
10.1006/geno.1996.0030subject
Has Abstractpub_date
1996-01-15 00:00:00pages
185-92issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(96)90030-0journal_volume
31pub_type
杂志文章相关文献
GENOMICS文献大全abstract::The molecular mechanism involved in BmNPV resistance was investigated using a genome wide microarray in midgut tissue of Indian silkworm Bombyx mori. In resistant race (Sarupat), 735 genes up-regulated and 589 genes down-regulated at 12 h post BmNPV infection. Similarly, in case of susceptible race (CSR-2), 2183 genes...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2015.09.002
更新日期:2015-12-01 00:00:00
abstract::MicroRNAs (miRNAs) have been prevalently studied in plants, animals, and viruses. However, recent studies show evidences of miRNA-like RNAs (milRNAs) in fungi as well. It is known that after successful infection, pathogens hijack the host machinery and use it for their own growth and multiplication. Alternatively, res...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.05.021
更新日期:2020-09-01 00:00:00
abstract::Physical maps of the human genome are being constructed by many groups using a mapping strategy that relies on the development of sequence-tagged sites (STSs). Thousands of physically mapped STSs, representing hundreds of kilobases (kb) of unique human DNA sequence, have been generated by these efforts. Since sequence...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0019
更新日期:1996-01-01 00:00:00
abstract::Acid sphingomyelinase (ASM; HGMW-approved symbol, SMPD1) is the lysosomal phosphodiesterase that hydrolyzes sphingomyelin to ceramide and phosphocholine. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). The full-length cDNA encoding human ASM has been isolated and characterize...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90366-z
更新日期:1992-02-01 00:00:00
abstract::Examination of mouse strains with a slit lamp and indirect ophthalmoscopy revealed that strain CBA/CaGnLe has a white cataract obvious at weaning age. It soon progresses to a large white nuclear cataract with mild cortical changes. Crosses with C57BL/6J showed that this is inherited as a single recessive fully penetra...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0439
更新日期:1996-08-15 00:00:00
abstract::Several mammalian sialidases have been cloned so far and here we describe the identification and expression of a new member of the human sialidase gene family. The NEU4 gene, identified by searching sequence databases for entries showing homologies to the human cytosolic sialidase NEU2, maps in 2q37 and encodes a 484-...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.08.019
更新日期:2004-03-01 00:00:00
abstract::Point mutations in the factor VIII gene are responsible for the majority of cases of hemophilia A, and only a small fraction of these mutations can be recognized by restriction endonuclease analysis. We have now used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize single nucleotid...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90569-g
更新日期:1990-02-01 00:00:00
abstract::Human DAB2IP (for DAB2 interaction protein) is a novel member of the RasGTPase-activating protein family. It interacts directly with DAB2, which suppresses growth of many cancer types. We demonstrated that DAB2IP is often downregulated in human prostate cancer cell lines. The predicted DAB2IP protein (967 amino acids)...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2002.6739
更新日期:2002-04-01 00:00:00
abstract::A novel method for identifying DNA point mutations has been developed by using mismatch repair enzymes. The high specificity of the Escherichia coli MutY protein has permitted the development of a reliable and sensitive method for the detection and characterization of point mutations in the human genome. The MutY prot...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80213-7
更新日期:1992-10-01 00:00:00
abstract::Type A receptors of gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter, contain alpha, beta, delta, gamma, and rho subunits. The gamma subunit has four subtypes: gamma1, gamma2, gamma3, andgamma4. GABA(A) receptor-associated protein (GABARAP) was previously demonstrated to act as a linker protein between m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6555
更新日期:2001-06-15 00:00:00
abstract::Using library to library cross-screening we have identified a number of genomic clones that harbor X-linked sequences expressed in the human choroid/retina. We describe the characterization of one of these, designated XEH.8 (DXS542), which is localized to Xp11.3-q12. Isolation, partial sequencing, and Northern analysi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1096
更新日期:1993-03-01 00:00:00
abstract::To identify genetic determinants relevant to non-insulin-dependent diabetes mellitus (NIDDM), we performed a genome-wide analysis for quantitative trait loci (QTLs) using 359 backcross progeny of the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. The OLETF strain is a well-studied animal model of obese NIDDM, with fea...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5789
更新日期:1999-06-15 00:00:00
abstract::Odorant receptors (ORs) and vomeronasal receptors (V1Rs and V2Rs) are large superfamilies of chemosensory receptors. As an extension of previous research using the 2001 Celera mouse genome assembly, we analyzed OR and V1R genes in the 2002 public mouse genome assembly. We identified 1403 OR genes (1068 potentially int...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.10.009
更新日期:2004-05-01 00:00:00
abstract::NotI and EagI boundary libraries were constructed for human chromosome 21. One hundred forty-seven clones were isolated from the somatic cell hybrid 72532X-6 and localized using a hybrid mapping panel. After identification of those clones, which were isolated more than once, as well as those probes derived from a prev...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90497-3
更新日期:1991-05-01 00:00:00
abstract::By searching the Expressed Sequence Tag database, a full-length cDNA for a novel human CC chemokine was cloned. This cDNA encoded a 94-amino-acid protein with a putative signal peptide of 26 amino acids. The deduced mature protein had the four conserved cysteine residues characteristic of CC chemokines and showed 44% ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5837
更新日期:1999-06-15 00:00:00
abstract::SCG10 is a neuronal growth-associated protein that shares an amino acid sequence similarity with an 18- to 19-kDa phosphoprotein named stathmin (also called p19, p18, Op18, pp17, prosolin, pp20, 19K, and leukemia-associated phosphoprotein, Lap18), which is more broadly expressed in a variety of cell types of the neura...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1477
更新日期:1993-11-01 00:00:00
abstract::The sequence of 20,114 bp of DNA including the human glucose-6-phosphate dehydrogenase (G6PD) gene was determined. The region included a prominent CpG island, starting about 680 nucleotides upstream of the transcription start site, extending about 1050 nucleotides downstream of the start site, and ending just at the s...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90465-q
更新日期:1991-07-01 00:00:00
abstract::For genetic linkage analysis of Japanese flounder, 160 doubled haploids (DH) were artificially produced using mitotic gynogenesis and were genotyped for 458 simple sequence repeat (SSR) markers, 101 of which show distortional segregation. The genetic linkage map was constructed by modifying recombination fractions bet...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.08.006
更新日期:2018-01-01 00:00:00
abstract::Fluorescence in situ hybridization was used to establish the order of, and to estimate genomic distances among, members of the carcinoembryonic antigen (CEA) and pregnancy-specific glycoprotein (PSG) subgroups on chromosome 19. Fluorescence in situ hybridization to metaphase chromosomes localized the PSG subgroup telo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90308-f
更新日期:1992-04-01 00:00:00
abstract::Portions of 16 chromosome 21 NotI linking clones were sequenced. These linking clone sequences represent sequence-tagged restriction sites that are potentially useful for finding genes and for finer genome mapping and sequencing. All of the clones were G+C rich (54 to 83%). CpG and GpC dinucleotide frequencies were ve...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1455
更新日期:1993-11-01 00:00:00
abstract::Treatment for lower-grade gliomas (LGG) has been challenging. Though emerging approaches such as immunotherapy is promising, it is still faced with immune tolerance, an obstacle that may be overcome by targeting autophagy-related (ATG) genes. After identifying three differentially expressed ATG genes (RIPK2, MUL1 and ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.10.008
更新日期:2020-10-15 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract. The loss and gain of miRNA function promote cancer development through various mechanisms. RNA sequencing (RNA-seq) and miRNAs sequencing data from the Cancer Genome Atlas (TCGA) was used to show the dysfunctional miRN...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.12.002
更新日期:2021-01-01 00:00:00
abstract::A novel gene, TEGT (testis enhanced gene transcript), has been identified in humans. It does not belong to any known gene family of vertebrates. The deduced amino acid sequence of the gene and a bacterial protein of unknown function show low but significant homology and very similar hydrophobicity profiles. Two differ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1145
更新日期:1995-07-20 00:00:00
abstract::Identifying physiological and transcriptomic changes can provide insights into the effects of drier air humidity stress on plants. In this study, we selected 6-month-old seedlings of Pterocarya stenoptera as study materials and used physiological index detection and transcriptome sequencing to investigate the adaptati...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.09.027
更新日期:2020-11-01 00:00:00
abstract::Hes2 encodes a mammalian basic helix-loop-helix transcriptional repressor homologous to the products of Drosophila hairy and Enhancer of split. Here, we isolated and characterized the mouse Hes2 gene. This gene consists of four exons, and all the introns are located within the protein-coding region at positions homolo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5213
更新日期:1998-04-01 00:00:00
abstract::A comparison of the murine and human junB loci reveals nine regions of distal 5'- and 3'-flanking DNA that exhibit greater than 72% sequence identity. A large fraction (over 50%) of the junB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1135
更新日期:1995-07-20 00:00:00
abstract::Core binding factor (CBF) is a heterodimeric transcription factor composed of two distinct subunits. The monomeric beta subunit is ubiquitously expressed, whereas expression of the three alpha subunits isolated previously seems to be restricted mainly to hematopoietic tissues. To isolate additional alpha genes, degene...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80185-o
更新日期:1995-04-10 00:00:00
abstract::Friedreich ataxia results from frataxin insufficiency caused by repeat expansion in intron 1 of the frataxin gene. Since the coding sequence is unchanged, the potential exists to ameliorate symptoms by increasing frataxin promoter activity. We therefore defined the minimal frataxin promoter in humans. Despite the fact...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.10.013
更新日期:2005-02-01 00:00:00
abstract::Laboratory mouse strains are known to have emerged from recent interbreeding between individuals of Mus musculus isolated populations. As a result of this breeding history, the collection of polymorphisms observed between laboratory mouse strains is likely to harbor the effects of natural selection between reproductiv...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.02.004
更新日期:2010-04-01 00:00:00
abstract::A NotI end clone library has been constructed from a human-hamster hybrid cell line containing only human chromosome 11. Fifty-one NotI clones were chosen to characterize the library. The majority of NotI clones hybridize to small 15- to 200-kb fragments and have proven to be valuable for chromosome 11 physical mappin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1120
更新日期:1993-03-01 00:00:00