当前位置： SCI文献检索 > PHARMACEUTICAL RESEARCH期刊下所有文献 > A novel topoisomerase II poison GL331 preferentially induces DNA cleavage at (C/G)T sites and can cause telomere DNA damage.

A novel topoisomerase II poison GL331 preferentially induces DNA cleavage at (C/G)T sites and can cause telomere DNA damage.

Abstract：

PURPOSE:Topoisomerase II (Topo II) preferentially cuts DNA at alternating purine-pyrimidine repeats. Different Topo II poisons may affect Topo II to produce distinct drug-specific DNA cleavage patterns. GL331 is a new podophyllotoxin derivative exhibiting potent Topo II-poisoning activity. Therefore, the sequence selectivity of GL331-induced DNA cleavage was determined. METHODS:Human gastric adenocarcinoma SC-M1 cells were treated with GL331, and the resultant DNA fragments were isolated by SDS-K+ precipitation. These DNA fragments were further cloned and sequenced to exhibit GL331-induced DNA cleavage sites. In addition, the telomere damage was detected by Southern blot analyses using a (TTAGGG)4 probe. GL331's effect on telomerase was examined using the TRAP assay. RESULTS:The selective sequences of GL331-induced DNA cleavage were analyzed. The first nucleotide 3'-terminal to the cleavage sites was preferentially C or G and followed by the second nucleotide T. More than 50% of GL331-induced DNA cleavage fragments exhibited AT-rich sequences in the first 20 nucleotides. In addition, the telomeric damage was observed both from GL331-treated SC-M1 cells and in vitro incubation of genomic DNA with GL331 and purified human Topo II. Although GL331 treatment reduced cellular telomerase activity, in vitro reaction data suggested that GL331 was not a telomerase inhibitor. CONCLUSION:GL331 preferentially induced Topo II-mediated DNA cleavage at (C/G)T sites. Because the telomeric repeat sequence contains GL331's GT preference site, the telomere was identified as one of the targets of GL331-induced DNA damage.

journal_name

Pharm Res

journal_title

Pharmaceutical research

authors

Lee CC,Huang TS

doi

10.1023/a:1011048831698

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

846-51

issue

6

eissn

0724-8741

issn

1573-904X

journal_volume

18

pub_type

杂志文章

相关文献

PHARMACEUTICAL RESEARCH文献大全
  • Aerosol dispersion of respirable particles in narrow size distributions produced by jet-milling and spray-drying techniques.

    abstract：PURPOSE:To examine the effect of particle size and morphology on aerosol dispersion using jet-milled and spray-dried mannitol particles in narrow size distributions within the respirable range. METHODS:Particle size and morphology were examined by laser diffraction and scanning electron microscopy, respectively. Aeros...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/b:pham.0000033007.27278.60

    authors： Louey MD,Van Oort M,Hickey AJ

    更新日期：2004-07-01 00:00:00

  • Supramolecular Chitosan Micro-Platelets Synergistically Enhance Anti-Candida albicans Activity of Amphotericin B Using an Immunocompetent Murine Model.

    abstract：PURPOSE:The aim of this work is to design new chitosan conjugates able to self-organize in aqueous solution in the form of micrometer-size platelets. When mixed with amphotericin B deoxycholate (AmB-DOC), micro-platelets act as a drug booster allowing further improvement in AmB-DOC anti-Candida albicans activity. METH...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-017-2117-3

    authors： Grisin T,Bories C,Bombardi M,Loiseau PM,Rouffiac V,Solgadi A,Mallet JM,Ponchel G,Bouchemal K

    更新日期：2017-05-01 00:00:00

  • Irinotecan Alters the Disposition of Morphine Via Inhibition of Organic Cation Transporter 1 (OCT1) and 2 (OCT2).

    abstract：PURPOSE:The organic cation transporters (OCTs) and multidrug and toxin extrusions (MATEs) together are regarded as an organic cation transport system critical to the disposition and response of many organic cationic drugs. Patient response to the analgesic morphine, a characterized substrate for human OCT1, is highly v...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-018-2526-y

    authors： Zhu P,Ye Z,Guo D,Xiong Z,Huang S,Guo J,Zhang W,Polli JE,Zhou H,Li Q,Shu Y

    更新日期：2018-10-25 00:00:00

  • Lipid absorption triggers drug supersaturation at the intestinal unstirred water layer and promotes drug absorption from mixed micelles.

    abstract：PURPOSE:To evaluate the potential for the acidic intestinal unstirred water layer (UWL) to induce drug supersaturation and enhance drug absorption from intestinal mixed micelles, via the promotion of fatty acid absorption. METHODS:Using a single-pass rat jejunal perfusion model, the absorptive-flux of cinnarizine and ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-013-1104-6

    authors： Yeap YY,Trevaskis NL,Porter CJ

    更新日期：2013-12-01 00:00:00

  • A novel oligodeoxynucleotide inhibitor of thrombin. II. Pharmacokinetics in the cynomolgus monkey.

    abstract：PURPOSE:To determine the pharmacokinetics of GS-522, an oligodeoxynucleotide (GGTTGGTGTGGTTGG) inhibitor of thrombin, after constant infusion and bolus administration in the cynomolgus monkey. METHODS:Using a stability indicating HPLC method, the GS-522 plasma concentration versus time data were obtained after constan...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016295907266

    authors： Lee WA,Fishback JA,Shaw JP,Bock LC,Griffin LC,Cundy KC

    更新日期：1995-12-01 00:00:00

  • Influence of gallate esterification on the activity of procyanidin B2 in androgen-dependent human prostate carcinoma LNCaP cells.

    abstract：PURPOSE:Present study assessed the influence of gallate esterification on the anti-cancer activity of procyanidin B2 (B2) in androgen-dependent human prostate carcinoma LNCaP cells employing B2-3,3'-di-O-gallate (B2-G(2)), two mono-gallate esters B2-3-O-gallate (B2-3G) and B2-3'-O-gallate (B2-3'G) and the parent compou...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-009-0037-6

    authors： Chou SC,Kaur M,Thompson JA,Agarwal R,Agarwal C

    更新日期：2010-04-01 00:00:00

  • EGFR Targeted Theranostic Nanoemulsion for Image-Guided Ovarian Cancer Therapy.

    abstract：PURPOSE:Platinum-based therapies are the first line treatments for most types of cancer including ovarian cancer. However, their use is associated with dose-limiting toxicities and resistance. We report initial translational studies of a theranostic nanoemulsion loaded with a cisplatin derivative, myrisplatin and pro-a...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-015-1660-z

    authors： Ganta S,Singh A,Kulkarni P,Keeler AW,Piroyan A,Sawant RR,Patel NR,Davis B,Ferris C,O'Neal S,Zamboni W,Amiji MM,Coleman TP

    更新日期：2015-08-01 00:00:00

  • A novel method for the synthesis of kyotorphin, Tyr-Arg, and 3H-Tyr-Arg, catalyzed by tyrosyl-tRNA synthetase from Bacillus stearothermophilus.

    abstract：:A novel method of dipeptide synthesis is described that can be carried out in aqueous solution and does not require complicated protecting and deprotecting procedures. An analgesic neuropeptide named kyotorphin, H-Tyr-Arg-OH, was synthesized from unprotected tyrosine and arginine in a new enzymatic reaction catalyzed ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016479305603

    authors： Kitabatake S,Tsurutani R,Nakajima H,Tomita K,Yoshihara Y,Ueda H,Takagi H,Imahori K

    更新日期：1987-04-01 00:00:00

  • Dependency of gastrointestinal toxicity on release rate of tiaprofenic acid: a novel pharmacokinetic-pharmacodynamic model.

    abstract：PURPOSE:To test the hypothesis that modification of release pattern of nonsteroidal anti-inflammatory drugs (NSAIDs) formulations shifts gastrointestinal (GI) toxicity of the drugs from the upper GI region to the distal intestine. METHODS:We assessed tiaprofenic acid (TA)-induced upper and lower increased GI permeabil...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1018887216098

    authors： Vakily M,Khorasheh F,Jamali F

    更新日期：1999-01-01 00:00:00

  • In vivo effects of glycyl-glutamate and glycyl-sarcosine on gabapentin oral absorption in rat.

    abstract：PURPOSE:The objective of this study was to evaluate the in vivo consequences of glycyl-glutamate coadministration on gabapentin oral absorption. METHODS:Rats were administered gabapentin (10 mg/kg plus radiotracer) by gastric gavage, in the absence and presence of dipeptides, and by intravenous administration. Serial ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-007-9272-x

    authors： Nguyen TV,Fleisher D,Smith DE

    更新日期：2007-08-01 00:00:00

  • Development of a Two-Dimensional Model for Predicting Transdermal Permeation with the Follicular Pathway: Demonstration with a Caffeine Study.

    abstract：PURPOSE:The development of a new two-dimensional (2D) model to predict follicular permeation, with integration into a recently reported multi-scale model of transdermal permeation is presented. METHODS:The follicular pathway is modelled by diffusion in sebum. The mass transfer and partition properties of solutes in li...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-017-2209-0

    authors： Kattou P,Lian G,Glavin S,Sorrell I,Chen T

    更新日期：2017-10-01 00:00:00

  • Heat-treated Fungizone retains amphotericin B antifungal activity without renal toxicity in rats infected with Aspergillus fumigatus.

    abstract：PURPOSE:The purpose of this study was to assess the antifungal activity and renal and hepatic toxicity of amphotericin B (AmpB) following administration of Fungizone (FZ) and a heat-treated form of FZ (HFZ) to rats infected with Aspergillus fumigatus. METHODS:Infected rats were administered FZ and HFZ at a dosing regi...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/b:pham.0000041449.46054.b4

    authors： Sivak O,Bartlett K,Wasan KM

    更新日期：2004-09-01 00:00:00

  • Development of an Improved Inhalable Powder Formulation of Pirfenidone by Spray-Drying: In Vitro Characterization and Pharmacokinetic Profiling.

    abstract：PURPOSE:Previously, a respirable powder (RP) formulation of pirfenidone (PFD) was developed for reducing phototoxic risk; however, PFD-RP demonstrated unacceptable in vitro inhalation performance. The present study aimed to develop a new RP system of PFD with favorable inhalation properties by spray-drying method. MET...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-016-1887-3

    authors： Seto Y,Suzuki G,Leung SS,Chan HK,Onoue S

    更新日期：2016-06-01 00:00:00

  • Relationship between drug percolation threshold and particle size in matrix tablets.

    abstract：PURPOSE:Since a previous qualitative study carried out by us showed the existence of an important influence of the particle size on the percolation thresholds and taking into account that the existing theoretical models can only provide qualitative explanation to this influence, the purpose of this work is to carry out...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016088424993

    authors： Caraballo I,Millan M,Rabasco AM

    更新日期：1996-03-01 00:00:00

  • Exploring Variation in Known Pharmacogenetic Variants and its Association with Drug Response in Different Mexican Populations.

    abstract：PURPOSE:Information on genetic variants that affect the pharmacokinetics and pharmacodynamics (PK/PD) of drugs in different populations from Mexico is still an ongoing endeavor. Here, we investigate allele frequencies on pharmacogenetic targets in Mexican Mestizos and Natives from three different States and its associa...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-016-1990-5

    authors： Gonzalez-Covarrubias V,Martínez-Magaña JJ,Coronado-Sosa R,Villegas-Torres B,Genis-Mendoza AD,Canales-Herrerias P,Nicolini H,Soberón X

    更新日期：2016-11-01 00:00:00

  • High sensitivity differential scanning calorimetry study of DNA-cationic liposome complexes.

    abstract：PURPOSE:To investigate plasmid DNA interactions with liposomes prepared from dimyristoylglyceroethylphosphocholine (EDMPC) and DOPE using high sensitivity differential scanning calorimetry (HSDSC). MATERIALS AND METHODS:Large unilamellar liposomes of EDMPC with DOPE (mol ratio 0-50%) were prepared. Plasmid DNA was add...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-007-9325-1

    authors： Saunders M,Taylor KM,Craig DQ,Palin K,Robson H

    更新日期：2007-10-01 00:00:00

  • Population Pharmacokinetics (PK) and Pharmacodynamics (PD) Analysis of LY3015014, a Monoclonal Antibody to Protein Convertase Subtilisin/Kexin Type 9 (PCSK9) in Healthy Subjects and Hypercholesterolemia Patients.

    abstract：PURPOSE:LY3015014 is a humanized immunoglobulin G4 (IgG4) monoclonal antibody that binds to the catalytic domain of PCSK9 and reduce low-density lipoprotein cholesterol (LDL-C) in patients with hypercholesterolemia that is poorly controlled by maximally tolerated statin therapy. The objective of this pharmacokinetic/ph...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-016-2054-6

    authors： Shen T,James DE,Krueger KA

    更新日期：2017-01-01 00:00:00

  • Role of phosphatidylserine in the cellular and subcellular lung distribution of quinidine in rats.

    abstract：:The role of phosphatidylserine in the cellular and subcellular lung distribution of quinidine was investigated in rats, since quinidine was found to bind preferentially to phosphatidylserine. The concentration of phosphatidylserine in the cellular and subcellular fractions was determined after separation by two-dimens...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015985511653

    authors： Nishiura A,Murakami T,Higashi Y,Yata N

    更新日期：1988-04-01 00:00:00

  • Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy.

    abstract：PURPOSE:Adjuvants are required to ensure the efficacy of subunit vaccines. Incorporating molecular immunopotentiators within particles could overcome drawbacks of molecular adjuvants (such as solubility and toxicity), and improve adjuvanticity of particles, achieving stronger adjuvant activity. Aim of this study is to ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-015-1666-6

    authors： Zhang W,Wang L,Yang T,Liu Y,Chen X,Liu Q,Jia J,Ma G

    更新日期：2015-09-01 00:00:00

  • Use of glancing angle X-ray powder diffractometry to depth-profile phase transformations during dissolution of indomethacin and theophylline tablets.

    abstract：PURPOSE:The purpose of this study was (i) to develop glancing angle x-ray powder diffractometry (XRD) as a method for profiling phase transformations as a function of tablet depth; and (ii) to apply this technique to (a) study indomethacin crystallization during dissolution of partially amorphous indomethacin tablets a...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/b:pham.0000012163.89163.f8

    authors： Debnath S,Predecki P,Suryanarayanan R

    更新日期：2004-01-01 00:00:00

  • Electroanalytical characteristics of the cardiotonics, enoximone and piroximone.

    abstract：:Enoximone and piroximone are cardiotonic agents for use in patients with congestive heart failure. Electroanalytical studies revealed that the dihydroimidazolone functionality was oxidizable and that this property can be analytically useful. The compounds undergo a two-electron, irreversible oxidation in neutral to ac...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015811830830

    authors： Housmyer CL,Lewis RL Jr

    更新日期：1991-07-01 00:00:00

  • A mechanism enhancing macromolecule transport through paracellular spaces induced by Poly-L-Arginine: Poly-L-Arginine induces the internalization of tight junction proteins via clathrin-mediated endocytosis.

    abstract：PURPOSE:Poly-L-arginine (PLA) enhances the paracellular permeability of the Caco-2 cell monolayer to hydrophilic macromolecules by disappearance of tight junction (TJ) proteins from cell-cell junctions. However, the mechanism of the disappearance of TJ proteins in response to PLA has been unclear. In this study, we inv...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-014-1324-4

    authors： Yamaki T,Kamiya Y,Ohtake K,Uchida M,Seki T,Ueda H,Kobayashi J,Morimoto Y,Natsume H

    更新日期：2014-09-01 00:00:00

  • Hyaluronan-based nanocarriers with CD44-overexpressed cancer cell targeting.

    abstract：PURPOSE:The objective of the work was to evaluate the potential of hyaluronan-based nanoparticles as tumor-targeting nano-systems for CD44-overexpressed cancer therapy. METHODS:The synthesized amphiphilic cholesteryl succinoyl hyaluronan (Chol-Suc-HA) conjugates self-assembled into docetaxel(DTX)-loaded nanoparticles ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-014-1393-4

    authors： Song S,Qi H,Xu J,Guo P,Chen F,Li F,Yang X,Sheng N,Wu Y,Pan W

    更新日期：2014-11-01 00:00:00

  • Rapid doxorubicin efflux from the nucleus of drug-resistant cancer cells following extracellular drug clearance.

    abstract：PURPOSE:Following extracellular drug clearance, we analyzed the rate of doxorubicin efflux from the nucleus of three human leukemic cells (K562, Molt4 and CCRF-CEM) and related it to their differential sensitivity to this drug, after a short drug pulse. RESULTS:For many pulse-chase regimes, K562 cell viability was lea...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-007-9369-2

    authors： Chen VY,Posada MM,Zhao L,Rosania GR

    更新日期：2007-11-01 00:00:00

  • Novel Approach for the Bioequivalence Assessment of Topical Cream Formulations: Model-Based Analysis of Tape Stripping Data Correctly Concludes BE and BIE.

    abstract：PURPOSE:The purpose of this study was (a) to suggest a novel dermatopharmacokinetic (DPK) approach from which pharmacokinetic parameters relevant to the bioequivalence (BE) assessment of a topical formulation can be deduced while circumventing the need for numerous measurements and assumptions, and (b) to investigate w...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-019-2724-2

    authors： Ozdin D,Kanfer I,Ducharme MP

    更新日期：2020-01-02 00:00:00

  • Route of administration and sex differences in the pharmacokinetics of aspirin, administered as its lysine salt.

    abstract：:One thousand milligrams of aspirin, as its lysine salt was administered intravenously, orally, and intramuscularly to nine male and nine female young healthy adult volunteers. After intravenous injection mean (+/- SD) values of clearance, steady-state volume of distribution, and terminal half-life were 12.2 +/- 2.2 ml...

    journal_title：Pharmaceutical research

    pub_type： 临床试验,杂志文章

    doi：10.1023/a:1015978104017

    authors： Aarons L,Hopkins K,Rowland M,Brossel S,Thiercelin JF

    更新日期：1989-08-01 00:00:00

  • A study on structural and diffusion properties of porcine stratum corneum based on very small angle neutron scattering data.

    abstract：PURPOSE:Generation of valuable information about the biphasic geometrical configuration of porcine stratum corneum from Very Small Angle Neutron Scattering (VSANS) data and investigation of its effect on the corresponding effective diffusivity. METHODS:Spectra of porcine stratum corneum are mathematically transformed ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1026453628800

    authors： Charalambopoulou GC,Karamertzanis P,Kikkinides ES,Stubos AK,Kanellopoulos NK,Papaioannou AT

    更新日期：2000-09-01 00:00:00

  • Accurate determination of skin flux from flow-through diffusion cell data.

    abstract：PURPOSE:The goal of this investigation was to demonstrate whether the intrinsic flux of a drug diffusing across a membrane mounted in a flow-through diffusion cell may be accurately and easily determined by accounting for the accumulation in the receiver chamber. METHODS:Mathematical modeling, applied to transdermal d...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016216611809

    authors： Harrison DJ,Knutson K

    更新日期：1995-12-01 00:00:00

  • Neotame anhydrate polymorphs. II: Quantitation and relative physical stability.

    abstract：PURPOSE:To study the relative thermodynamic and kinetic stabilities of neotame anhydrate polymorphs A, D, F, and G, and to develop a quantitative method for analyzing polymorphic mixtures of A and G by powder X-ray diffractometry (PXRD). METHODS:Based on the melting points, heats of fusion, and densities of the four p...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1020334305038

    authors： Dong Z,Munson EJ,Schroeder SA,Prakash I,Grant DJ

    更新日期：2002-09-01 00:00:00

  • Utilization of an amorphous form of a water-soluble GPIIb/IIIa antagonist for controlled release from biodegradable microspheres.

    abstract：PURPOSE:We prepared injectable microspheres for controlled release of TAK-029, a water-soluble GPIIb/IIIa antagonist and discussed the characteristics of controlled release from microspheres. METHODS:Copoly(dl-lactic/glycolic)acid (PLGA) microspheres were used for controlled release of TAK-029 [4-(4-amidinobenzoylglyc...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1012190304074

    authors： Takada S,Kurokawa T,Miyazaki K,Iwasa S,Ogawa Y

    更新日期：1997-09-01 00:00:00