Spleen autotransplantation in mice: a novel experimental model for immunology study.

Abstract:

:The aim of the experimental model to develop a spleen transplant model in mice to study the role of spleen in autoimmune and transplant rejection. After a midline incision, splenectomy was performed. Four tiny segments were cut from the removed spleen and were rinsed at room temperature in physiological salt solution. The greater omentum was lifted and four omental pockets were created; four thin segments were then placed into the "nests," subsequently marked, and fixed using 8-0 suture. The abdomen was then closed. The duration of the survival time was different among the nine groups (n = 3-3). Tissue samples were taken from the marked areas for histological examination stained with hematoxylin and eosin (H&E). H&E staining demonstrated large, well-circumscribed splenic nests with lymphoid zone and red pulp and well-formed trabecules in the spleen. Among the possible applications of this novel model is the ability to study the role of spleen in autoimmune and organ rejection.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Mikó I,Bráth E,Furka I,Kovács J,Kelvin D,Zhong R

doi

10.1002/micr.1026

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

140-2

issue

4

eissn

0738-1085

issn

1098-2752

pii

10.1002/micr.1026

journal_volume

21

pub_type

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