Abstract:
:The aim of the experimental model to develop a spleen transplant model in mice to study the role of spleen in autoimmune and transplant rejection. After a midline incision, splenectomy was performed. Four tiny segments were cut from the removed spleen and were rinsed at room temperature in physiological salt solution. The greater omentum was lifted and four omental pockets were created; four thin segments were then placed into the "nests," subsequently marked, and fixed using 8-0 suture. The abdomen was then closed. The duration of the survival time was different among the nine groups (n = 3-3). Tissue samples were taken from the marked areas for histological examination stained with hematoxylin and eosin (H&E). H&E staining demonstrated large, well-circumscribed splenic nests with lymphoid zone and red pulp and well-formed trabecules in the spleen. Among the possible applications of this novel model is the ability to study the role of spleen in autoimmune and organ rejection.
journal_name
Microsurgeryjournal_title
Microsurgeryauthors
Mikó I,Bráth E,Furka I,Kovács J,Kelvin D,Zhong Rdoi
10.1002/micr.1026subject
Has Abstractpub_date
2001-01-01 00:00:00pages
140-2issue
4eissn
0738-1085issn
1098-2752pii
10.1002/micr.1026journal_volume
21pub_type
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