Establishment and characterization of a novel acute promyelocytic leukemia cell line (UF-1) with retinoic acid-resistant features.

Abstract:

:All-trans retinoic acid (RA) induces complete remission in a high proportion of patients with acute promyelocytic leukemia (APL). Nevertheless, most of these patients develop RA resistance and relapse. The mechanisms of RA resistance by APL cells are still unclear. To understand the characteristics of human leukemia, human leukemic cell lines are useful tools for study. APL cells have a strikingly low proliferation potential in vitro; thus, only one APL cell line has been established. We developed a novel APL cell line (UF-1) from a patient clinically resistant to all-trans RA. Cell surface markers in the UF-1 cells were positive for CD7, CD13, CD33, and CD38. Cytogenetic analyses revealed additional abnormalities, 46XX, add(1)(q44), add(6)(q12), add(7)(q36), t(15;17) (q21;q21). Molecular analyses showed a PML/RAR alpha fusion transcript. Sequence analysis of the RAR alpha gene in RA-resistant HL-60 cells disclosed a point mutation in codon 411 (C to T substitution), whereas UF-1 cells showed the normal sequence. All-trans RA did not change morphological features of the cell, NBT reduction activity, or their expression of CD11b antigens as determined by FACS analysis except at 10(-6) mol/L. RA also did not alter the growth curve of the cells as determined by the MTT assay. These findings suggest that the UF-1 cell is the first permanent cell line with spontaneous RA-resistant APL cells. This RA-resistant APL cell line may be a useful model for molecular studies on the block of leukemic cell differentiation and as a means to investigate the mechanisms of RA resistance.

journal_name

Blood

journal_title

Blood

authors

Kizaki M,Matsushita H,Takayama N,Muto A,Ueno H,Awaya N,Kawai Y,Asou H,Kamada N,Ikeda Y

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

1824-33

issue

5

eissn

0006-4971

issn

1528-0020

journal_volume

88

pub_type

杂志文章

相关文献

BLOOD文献大全
  • Factor VIII ectopically expressed in platelets: efficacy in hemophilia A treatment.

    abstract::Activated platelets release their granule content in a concentrated fashion at sites of injury. We examined whether ectopically expressed factor VIII in developing megakaryocytes would be stored in alpha-granules and whether its release from circulating platelets would effectively ameliorate bleeding in a factor VIIIn...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-05-1519

    authors: Yarovoi HV,Kufrin D,Eslin DE,Thornton MA,Haberichter SL,Shi Q,Zhu H,Camire R,Fakharzadeh SS,Kowalska MA,Wilcox DA,Sachais BS,Montgomery RR,Poncz M

    更新日期:2003-12-01 00:00:00

  • EB1, p150Glued, and Clasp1 control endothelial tubulogenesis through microtubule assembly, acetylation, and apical polarization.

    abstract::Vascular tube morphogenesis requires the establishment of endothelial cell (EC) apical-basal polarity in three-dimensional (3D) extracellular matrices. To date, there is little understanding of how EC polarity is controlled during these highly dynamic and rapid morphogenic events. We show that the microtubule tip comp...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2012-11-470179

    authors: Kim DJ,Martinez-Lemus LA,Davis GE

    更新日期:2013-04-25 00:00:00

  • Exposure of human megakaryocytes to high shear rates accelerates platelet production.

    abstract::Platelets originate from megakaryocytes (MKs) by cytoplasmic elongation into proplatelets. Direct platelet release is not seen in bone marrow hematopoietic islands. It was suggested that proplatelet fragmentation into platelets can occur intravascularly, yet evidence of its dependence on hydrodynamic forces is missing...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2009-03-209205

    authors: Dunois-Lardé C,Capron C,Fichelson S,Bauer T,Cramer-Bordé E,Baruch D

    更新日期:2009-08-27 00:00:00

  • Tissue factor-factor VIIa-specific up-regulation of IL-8 expression in MDA-MB-231 cells is mediated by PAR-2 and results in increased cell migration.

    abstract::Tissue factor (TF), the cellular receptor for factor VIIa (FVIIa), besides initiating blood coagulation, is believed to play an important role in tissue repair, inflammation, angiogenesis, and tumor metastasis. Like TF, the chemokine interleukin-8 (IL-8) is shown to play a critical role in these processes. To elucidat...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-10-3417

    authors: Hjortoe GM,Petersen LC,Albrektsen T,Sorensen BB,Norby PL,Mandal SK,Pendurthi UR,Rao LV

    更新日期:2004-04-15 00:00:00

  • Signaling through CD43 induces natural killer cell activation, chemokine release, and PYK-2 activation.

    abstract::Natural killer (NK) cell activation is the result of a balance between positive and negative signals triggered by specific membrane receptors. We report here the activation of NK cells induced through the transmembrane glycoprotein CD43 (leukosialin, sialophorin). Engagement of CD43 by specific antibodies stimulated t...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Nieto M,Rodríguez-Fernández JL,Navarro F,Sancho D,Frade JM,Mellado M,Martínez-A C,Cabañas C,Sánchez-Madrid F

    更新日期:1999-10-15 00:00:00

  • PECAM-1-dependent neutrophil transmigration is independent of monolayer PECAM-1 signaling or localization.

    abstract::Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), a tyrosine phosphoprotein highly expressed on endothelial cells and leukocytes, is an important component in the regulation of neutrophil transendothelial migration. Engagement of endothelial PECAM-1 activates tyrosine phosphorylation events and evokes prol...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2002-08-2396

    authors: O'Brien CD,Lim P,Sun J,Albelda SM

    更新日期:2003-04-01 00:00:00

  • Hepatocyte-targeted HFE and TFR2 control hepcidin expression in mice.

    abstract::Hereditary hemochromatosis is caused by mutations in the hereditary hemochromatosis protein (HFE), transferrin-receptor 2 (TfR2), hemojuvelin, hepcidin, or ferroportin genes. Hepcidin is a key iron regulator, which is secreted by the liver, and decreases serum iron levels by causing the down-regulation of the iron tra...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2009-09-245209

    authors: Gao J,Chen J,De Domenico I,Koeller DM,Harding CO,Fleming RE,Koeberl DD,Enns CA

    更新日期:2010-04-22 00:00:00

  • Two distinct categories of warm autoantibody reactivity with age-fractionated red cells.

    abstract::Using age-fractionated erythrocytes, warm autoantibodies can be classified into two distinct categories, depending on their reactivity with reticulocyte-enriched (younger) or reticulocyte-poor (older) red cell fractions. The strength of the direct antiglobulin test (DAT) on the age-fractionated red cells of 24 patient...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Branch DR,Shulman IA,Sy Siok Hian AL,Petz LD

    更新日期:1984-01-01 00:00:00

  • Platelet size in man.

    abstract::The shape and parameters of platelet size distributions were studied in 50 normal persons and 97 patients in order to test the proposed thesis that platelet size heterogeneity results mainly from aging in the circulation. This thesis was contradicted (1) by size distributions of age-homogeneous, newly-born cell popula...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Paulus JM

    更新日期:1975-09-01 00:00:00

  • Positron emission tomography in non-Hodgkin's lymphoma: assessment of chemotherapy with fluorodeoxyglucose.

    abstract::Positron emission tomography (PET) using F-18 fluorodeoxyglucose (FDG) was performed in non-Hodgkin's lymphoma (NHL), which is known to be highly responsive to chemotherapy, but also yields variable treatment results to answer the following questions: (1) What is the extent and time course of changes in FDG utilizatio...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Römer W,Hanauske AR,Ziegler S,Thödtmann R,Weber W,Fuchs C,Enne W,Herz M,Nerl C,Garbrecht M,Schwaiger M

    更新日期:1998-06-15 00:00:00

  • Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cells.

    abstract::Transcriptional control has been identified as a key mechanism regulating the formation and subsequent behavior of hematopoietic stem cells. We have used a comparative genomics approach to identify transcriptional regulatory elements of the LMO2 gene, a transcriptional cofactor originally identified through its involv...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2004-12-4755

    authors: Landry JR,Kinston S,Knezevic K,Donaldson IJ,Green AR,Göttgens B

    更新日期:2005-10-15 00:00:00

  • Acute hyperviscosity: syndromes and management.

    abstract::Plasma hyperviscosity is a rare complication of both monoclonal and polyclonal disorders associated with elevation of immunoglobulins. Asymptomatic patients with an elevation in the serum viscosity do not require plasma exchange, and the majority will have other indications for therapeutic intervention. For patients w...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2018-06-846816

    authors: Gertz MA

    更新日期:2018-09-27 00:00:00

  • Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets.

    abstract::Early in life, thymic export establishes the size and the diversity of the human naive T-cell pool. Yet, on puberty thymic activity drastically decreases. Because the overall size of the naive T-cell pool decreases only marginally during ageing, peripheral postthymic expansion of naive T cells has been postulated to a...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2008-02-139154

    authors: Kohler S,Thiel A

    更新日期:2009-01-22 00:00:00

  • Electrolyte and acid-base disturbances in the management of leukemia.

    abstract::Electrolyte disturbances in leukemia can be the result of the disease process or drug therapy. One group of electrolyte abnormalities is related to the stage of the leukemic process. Included in this group are newly diagnosed patients who may show elevated serum potassium, phosphorus, and magnesium--a result of their ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: O'Regan S,Carson S,Chesney RW,Drummond KN

    更新日期:1977-03-01 00:00:00

  • The histone methyltransferase Ezh2 is a crucial epigenetic regulator of allogeneic T-cell responses mediating graft-versus-host disease.

    abstract::Posttranscriptional modification of histones by methylation plays an important role in regulating Ag-driven T-cell responses. We have recently drawn correlations between allogeneic T-cell responses and the histone methyltransferase Ezh2, which catalyzes histone H3 lysine 27 trimethylation. The functional relevance of ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2013-05-505180

    authors: He S,Xie F,Liu Y,Tong Q,Mochizuki K,Lapinski PE,Mani RS,Reddy P,Mochizuki I,Chinnaiyan AM,Mineishi S,King PD,Zhang Y

    更新日期:2013-12-12 00:00:00

  • Paroxysmal nocturnal hemoglobinuria.

    abstract::Paroxysmal nocturnal hemoglobinuria (PNH) is a rare bone marrow failure disorder that manifests with hemolytic anemia, thrombosis, and peripheral blood cytopenias. The absence of two glycosylphosphatidylinositol (GPI)-anchored proteins, CD55 and CD59, leads to uncontrolled complement activation that accounts for hemol...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2014-02-522128

    authors: Brodsky RA

    更新日期:2014-10-30 00:00:00

  • Physical and functional association of Fc alpha R with protein tyrosine kinase Lyn.

    abstract::In this report, we show that the Src family nonreceptor protein tyrosine kinase (PTK) Lyn associates with aggregated IgA Fc receptor (Fc alpha R) in the monocytic cell line THP-1. Receptor aggregation and subsequent immunoprecipitation of receptor complexes with huIgA adsorbed to nitrocellulose particles shows that Ly...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Gulle H,Samstag A,Eibl MM,Wolf HM

    更新日期:1998-01-15 00:00:00

  • Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells.

    abstract::We confirmed that murine hematopoietic stem cells express the c-kit molecule but not lymphohematopoietic lineage markers. These lineage marker-negative c-kit-positive (Lin- c-kit+) cells were further divided according to the uptake of rhodamine-123 (Rh-123). Approximately 1,000 Lin- c-kit+ rhodamine-123dull cells, whi...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Okada S,Nagayoshi K,Nakauchi H,Nishikawa S,Miura Y,Suda T

    更新日期:1993-04-01 00:00:00

  • Accelerated telomere shortening in hematological lineages is limited to the first year following stem cell transplantation.

    abstract::Using quantitative fluorescence in situ hybridization and flow cytometry, the telomere length of telomere repeat sequences after stem cell transplantation (SCT) were measured. The study included the telomeres of peripheral blood monocytes that should reflect the length of telomeres in stem cells and the telomeres of T...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.v97.2.575

    authors: Rufer N,Brümmendorf TH,Chapuis B,Helg C,Lansdorp PM,Roosnek E

    更新日期:2001-01-15 00:00:00

  • Distinct functional properties of highly purified hematopoietic stem cells from mouse strains differing in stem cell numbers.

    abstract::We have previously demonstrated that young adult DBA/2 (DBA) mice have more stem cells than C57BL/6 (B6) mice, as measured in a cobblestone area-forming cell (CAFC) assay using unfractionated marrow. To study the nature of this difference, we have now compared the proliferative fate of single, highly enriched Sca-1(+)...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: de Haan G,Szilvassy SJ,Meyerrose TE,Dontje B,Grimes B,Van Zant G

    更新日期:2000-08-15 00:00:00

  • Longitudinal analysis of point mutations of the N-ras proto-oncogene in patients with myelodysplasia using archived blood smears.

    abstract::We performed a longitudinal analysis of point mutations of the N-ras proto-oncogene in patients with myelodysplasia and a follow-up of at least 2.5 years after diagnosis. Point mutations at codons 12, 13, and 61 of the N-ras oncogene were analyzed after in vitro amplification of N-ras specific sequences followed by do...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: van Kamp H,de Pijper C,Verlaan-de Vries M,Bos JL,Leeksma CH,Kerkhofs H,Willemze R,Fibbe WE,Landegent JE

    更新日期:1992-03-01 00:00:00

  • Cholesterol-modulating agents kill acute myeloid leukemia cells and sensitize them to therapeutics by blocking adaptive cholesterol responses.

    abstract::The mevalonate pathway produces many critical substances in cells, including sterols essential for membrane structure and isoprenoids vital to the function of many membrane proteins. 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase is a rate-limiting enzyme in the mevalonate pathway. Because cholesterol is a ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2002-07-2283

    authors: Li HY,Appelbaum FR,Willman CL,Zager RA,Banker DE

    更新日期:2003-05-01 00:00:00

  • Specific killing of multiple myeloma cells by (-)-epigallocatechin-3-gallate extracted from green tea: biologic activity and therapeutic implications.

    abstract::Epigallocatechin-3-gallate (EGCG), a polyphenol extracted from green tea, is an antioxidant with chemopreventive and chemotherapeutic actions. Based on its ability to modulate growth factor-mediated cell proliferation, we evaluated its efficacy in multiple myeloma (MM). EGCG induced both dose- and time-dependent growt...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2006-05-022814

    authors: Shammas MA,Neri P,Koley H,Batchu RB,Bertheau RC,Munshi V,Prabhala R,Fulciniti M,Tai YT,Treon SP,Goyal RK,Anderson KC,Munshi NC

    更新日期:2006-10-15 00:00:00

  • Adenovirus-mediated transfer of tissue-type plasminogen activator augments thrombolysis in tissue-type plasminogen activator-deficient and plasminogen activator inhibitor-1-overexpressing mice.

    abstract::Impaired fibrinolysis, resulting from increased plasminogen activator inhibitor-1 (PAI-1) or reduced tissue-type plasminogen activator (t-PA) plasma levels, may predispose the individual to subacute thrombosis in sepsis and inflammation. The objective of these studies was to show that adenovirus-mediated gene transfer...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Carmeliet P,Stassen JM,Van Vlaenderen I,Meidell RS,Collen D,Gerard RD

    更新日期:1997-08-15 00:00:00

  • Can thrombophilia predict recurrent catheter-related deep vein thrombosis in children?

    abstract::The role of thrombophilia testing in predicting catheter-related deep vein thrombosis (DVT) after an incident (ie, first) catheter-related DVT in children remains unclear. The present study investigated the association between thrombophilia and recurrent catheter-related DVT. Children with thrombophilia testing, perfo...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2017-10-811216

    authors: Avila ML,Amiri N,Stanojevic S,Vu TT,Barron K,Krol P,Yue N,Williams S,Brandão LR

    更新日期:2018-06-14 00:00:00

  • In vitro platelet abnormality in adenosine deaminase deficiency and severe combined immunodeficiency.

    abstract::The platelets of an infant with severe combined immune deficiency and adenosine deaminase deficiency showed markedly diminished responses to ADP-induced aggregation in vitro. This abnormality was corrected by the addition of purified adenosine deaminase in vitro. Exogenous adenosine added to platelet-rich plasma cause...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Lee CH,Evans SP,Rozenberg MC,Bagnara AS,Ziegler JB,Van der Weyden MB

    更新日期:1979-03-01 00:00:00

  • Epratuzumab with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone chemotherapy in patients with previously untreated diffuse large B-cell lymphoma.

    abstract::Approximately 60% of patients with diffuse large B-cell non-Hodgkin lymphoma (DLBCL) are curable with rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) chemoimmunotherapy. Epratuzumab (E) is an unlabeled anti-CD22 monoclonal antibody with efficacy in relapsed DLBCL. This phase 2 trial te...

    journal_title:Blood

    pub_type: 杂志文章,多中心研究

    doi:10.1182/blood-2011-02-336990

    authors: Micallef IN,Maurer MJ,Wiseman GA,Nikcevich DA,Kurtin PJ,Cannon MW,Perez DG,Soori GS,Link BK,Habermann TM,Witzig TE

    更新日期:2011-10-13 00:00:00

  • Dangerous small B-cell clones.

    abstract::The detection of a monoclonal immunoglobulin in serum or urine usually raises concerns about the size of the underlying B-cell-derived clone and possible systemic effects caused by its expansion. However, a small clone can synthesize a very toxic protein, producing devastating systemic damage and protean clinical pres...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2006-03-001164

    authors: Merlini G,Stone MJ

    更新日期:2006-10-15 00:00:00

  • A reduced sensitivity for activated protein C in the absence of factor V Leiden increases the risk of venous thrombosis.

    abstract::Activated protein C (APC) resistance caused by the factor V Leiden mutation is associated with an increased risk of venous thrombosis. We investigated whether a reduced response to APC, not due to the factor V point mutation, is also a risk factor for venous thrombosis. For this analysis, we used the Leiden Thrombophi...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: de Visser MC,Rosendaal FR,Bertina RM

    更新日期:1999-02-15 00:00:00

  • JAK2 inhibitors for myeloproliferative neoplasms: what is next?

    abstract::Since its approval in 2011, the Janus kinase 1/2 (JAK1/2) inhibitor ruxolitinib has evolved to become the centerpiece of therapy for myelofibrosis (MF), and its use in patients with hydroxyurea resistant or intolerant polycythemia vera (PV) is steadily increasing. Several other JAK2 inhibitors have entered clinical te...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2017-04-742288

    authors: Bose P,Verstovsek S

    更新日期:2017-07-13 00:00:00