Abstract:
:Careful titration of Vent polymerase activity allows efficient amplification of full-length plasmids (12 kb). The high processivity and fidelity of this enzyme made oligonucleotide-directed site-specific mutagenesis of plasmids a straight-forward process. Using only two primers, a mutagenic and a complementary, single-base mutants of recombinant plasmids were obtained consistently with > 90% efficiency from a single round of PCR. This procedure also made site-specific deletion, insertion, and several bases mutagenesis facile and efficient.
journal_name
Genome Resjournal_title
Genome researchauthors
Byrappa S,Gavin DK,Gupta KCdoi
10.1101/gr.5.4.404subject
Has Abstractpub_date
1995-11-01 00:00:00pages
404-7issue
4eissn
1088-9051issn
1549-5469journal_volume
5pub_type
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