Abstract:
BACKGROUND:PG490-88, a semisynthetic derivative of a novel compound PG490 (triptolide) purified from a Chinese herb (Tripterygium wilfordii Hook F), is effective in prevention of murine graft-versus-host disease (GVHD). METHODS:PG490-88 was administrated into recipients in a model (B10.D2 [H2d, Mls-2b, Mls-3b]-->BALB/c [H2d, Mls-2a, Mls-3a]) of lethal GVHD. Tolerance was evaluated by transplantation of neonatal hearts. The mechanisms of tolerance were studied. RESULTS:Host-specific tolerance was established in PG490-88-treated BALB/c recipients. Significant numbers of host reactive Vbeta3+ T cells (3.56+/-1.66% among CD4, 4.06+/-1.62% among CD8, P<0.0001 vs. normal BALB/c mice, P>0.05 vs. normal B10.D2 mice) were present in PG490-88-treated mice, suggesting that clonal deletion was not responsible for the observed tolerance. Spleen cells from PG490-88-treated mice could not respond to the host antigens measured by a popliteal lymph node weight gain assay. The unresponsiveness was unable to be overcome by supplementation of exogenous interleukin (IL)-2. Tolerant Vbeta3+ T cells obtained from PG490-88-treated mice proliferated normally to nonantigen-specific T cell receptor cross-linking. Neither antigen-specific nor nonantigen-specific suppressor cells were found in PG490-88-treated mice. The tolerant mice produced IL-4 rather than IL-2 and interferon (IFN)-gamma. CONCLUSIONS:Host-specific tolerance induced by PG490-88 in a murine bone marrow transplantation model is not due to deletion of alloreactive cells. Moreover, suppressor cells are not involved in the maintenance of tolerance. Rather, PG490-88 seems to lead to allogeneic tolerance either through the induction of a state of antigen-specific anergy of the responding T cells or through the induction of T-helper cell, type II (TH2) responses.
journal_name
Transplantationjournal_title
Transplantationauthors
Chen BJ,Chen Y,Cui X,Fidler JM,Chao NJdoi
10.1097/00007890-200201150-00022subject
Has Abstractpub_date
2002-01-15 00:00:00pages
115-21issue
1eissn
0041-1337issn
1534-6080journal_volume
73pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::Since the earliest days of transplantation, immunobiologists have sought means to prevent recognition and rejection of foreign tissue. The goal of these strategies is the retention of recipient immune function while selectively avoiding graft injury. Although considerable theoretical and technical problems remain, an ...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0b013e3181bcc93a
更新日期:2009-12-15 00:00:00
abstract:BACKGROUND:The efficiency of islet graft survival after intraportal implantation is compromised by host innate immune responses and the production of proinflammatory cytokines that cause acute cellular injury. This reaction activates intraislet nuclear factor-κB (NF-κB), causing production of gene products that have de...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182a4190e
更新日期:2013-11-27 00:00:00
abstract:BACKGROUND:It is well known that kidney transplant recipients with preformed lymphocytotoxic antibodies against HLA antigens have an increased graft rejection rate. However, the individual contribution of anti-HLA class I and class II antibodies to this phenomenon is poorly understood. We investigated the clinical rele...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200204270-00014
更新日期:2002-04-27 00:00:00
abstract::The expression of some genes has been comparatively studied in transplanted rat liver and in liver reperfused after ischemia in situ. Experiments on protein synthesis by tissue slices from cold-stored or transplanted livers show that rat livers that retain a good capacity for protein synthesis during storage undergo a...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199305000-00004
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:Black American heart transplant recipients receiving cyclosporine-based primary immunoprophylaxis suffer higher rates of allograft rejection with hemodynamic compromise, infections, and posttransplant coronary artery disease. We examined the hypothesis that a combination of tacrolimus and mycophenolate mofet...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-200212150-00014
更新日期:2002-12-15 00:00:00
abstract::Mixed lymphocyte culture--lymphocyte-mediated cytotoxicity (MLC-LMC) reactions by recipient spleen cells were strongly and specifically increased shortly after primarily vascularized heart grafts were placed in mice. Subsequently, in strain combinations in which eventual long-term graft survival occurred, the MLC-LMC ...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract:BACKGROUND:This study aims to verify the condition of recipients of solid organs from donors with central nervous system (CNS) tumors and determine the risk of disease transmission due to transplantation. METHODS:Twenty-eight brain-dead organ donors with CNS tumors and 91 recipients who received solid organs from Janu...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0000000000002994
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Nonspecific inflammatory damages occurring prior to organ transplantation reduce long-term graft survival. Here, we tested the beneficial effects of carbon monoxide (CO) induction by methylene chloride (MC). METHODS:Fischer-344 (F-344 Rat) or Dark Agouti (DA Rat) donor animals were either treated with MC fo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000232716.91887.c5
更新日期:2006-10-15 00:00:00
abstract::It has been postulated that the establishment of acquired, neonatal immunologic tolerance is due to a "deficit" in interleukin 2 (IL-2). To test this hypothesis, chickens were made immunologically tolerant to both major and minor histocompatibility antigens by transplantation of skin grafts onto newly hatched recipien...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198802000-00041
更新日期:1988-02-01 00:00:00
abstract::Controversy exists as to the type of cells present in the urine during renal allograft rejection. In order to resolve this controversy as well as to evaluate the value of urine sediment examination as a means of detecting AR, we quantitated the different cells present in urine during AR using an immunoperoxidase techn...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198903000-00017
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND:This study evaluates whether surgical strategies such as the portosystemic shunt and ischemic preconditioning can protect against hepatic and pulmonary injury associated with liver transplantation. METHODS:The effect of the portosystemic shunt, ischemic preconditioning, and both surgical procedures together...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000067529.82245.4E
更新日期:2003-07-27 00:00:00
abstract:BACKGROUND:An increase in serum alpha-glutathione S-transferase concentration (GST) has been shown to be a more sensitive and specific marker of hepatocellular damage than equivalent increases in transaminase activities. A randomized clinical trial of 60 liver transplants in 49 patients was carried out to assess the cl...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199711270-00013
更新日期:1997-11-27 00:00:00
abstract::Endothelial chimerism in transplanted organs is a fascinating phenomenon, indicative of a mechanism by which progenitor recipient cells replace the donor endothelium. It has been hypothesized that this replacement could lead to a decrease in alloreactivity and thus would positively influence graft outcome. However, re...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000231446.41051.98
更新日期:2006-07-15 00:00:00
abstract:BACKGROUND:Renal allograft rejection is associated with the expression of adhesion molecules on vascular endothelial and tubular epithelial cells. METHODS:To assess whether the number of cell adhesion molecules expressed in donor kidneys can predict early rejection or delayed graft function, kidney biopsies from 20 li...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200106150-00028
更新日期:2001-06-15 00:00:00
abstract::Hepatic artery thrombosis is a continuing source of morbidity and mortality following orthotopic liver transplantation. The cornerstone of therapy has been urgent retransplantation that is limited by organ availability. For this reason we developed a policy of urgent revascularization for allograft rescue. Hepatic art...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199101000-00013
更新日期:1991-01-01 00:00:00
abstract::The possible ability of the mixed-epidermal-cell-lymphocyte reaction (MECLR) to detect alloreactivities in HLA-identical MLR negative siblings has been investigated before grafting in 30 donor-recipients pairs and their families. Results indicate that recipients' epidermal cells (EC) can induce proliferative responses...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198603000-00007
更新日期:1986-03-01 00:00:00
abstract:BACKGROUND:Cyclosporine A (CsA)-induced chronic nephrotoxicity is characterized by renal dysfunction and interstitial fibrosis. Early and progressive renal macrophage influx, correlating with latter interstitial fibrotic areas, has been associated with CsA treatment. This study investigated the role of macrophages, the...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/tp.0b013e3181da0587
更新日期:2010-06-15 00:00:00
abstract:BACKGROUND:Most transplant centers in the United States, including our own, use creatinine clearance (Ccr) to estimate glomerular filtration rate (GFR) in potential living kidney donors. The purpose of this study was to evaluate our experience with estimation of donor GFR by Ccr and to explore other potential strategie...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200101270-00016
更新日期:2001-01-27 00:00:00
abstract::Second-set rejection is generally considered to be mediated by cytotoxic humoral antibodies. A few discordant data have been reported, however. To address this question, we have taken advantage of a model in which specific cytotoxic alloantibodies are not produced although transplantation cellular immunity develops. F...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199309000-00003
更新日期:1993-09-01 00:00:00
abstract::The question has not been previously investigated as to whether host lymphocytes infiltrate vascularized organ allografts in indiscriminate fashion, or whether they are retained selectively because of specific antigenic recognition sites on their surfaces. By using a dual cardiac allograft model in LEW rats, we have e...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197809000-00011
更新日期:1978-09-01 00:00:00
abstract::Monoclonal antibodies to CD3 have been shown to activate T cells in vivo and in vitro but have also been shown to render T cells anergic in vitro. In this study G4.18, a mouse IgG3 mAb, was produced that appeared to recognize CD3 by its binding to all peripheral T cells, including a population not recognized by mAb to...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199303000-00001
更新日期:1993-03-01 00:00:00
abstract::Experimental studies of vein homografts in rats have shown prolonged patency resulting from an attenuated immune response. This study examined the response to Golden Syrian hamster vein xenografts. While one group of Sprague-Dawley rats served as a control, a second group was sensitized with 5 X 10(6) viable hamster s...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198511000-00011
更新日期:1985-11-01 00:00:00
abstract::Two anti-H-2Ld sera were analyzed, BALB/c-H-2dm2 anti-BALB/cBy and (C3H X BALB/c-H-2dm2)F1 anti-BALB/cHe, the latter also containing anti-Qa antibodies. Their reaction patterns were compared with an anti-Qa serum (C3H X BALB/cBy)F1 anti-BALB/cHe. Four H-2 specificities could be detected by the anti-H-2Ld sera, two alr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198108000-00009
更新日期:1981-08-01 00:00:00
abstract::Transplant coronary artery disease and malignancy are the major limitations to survival after heart transplantation. In a randomized trial, mycophenolate mofetil (MMF) significantly improved 3-year survival over azathioprine, but the expected reduction in intracoronary ultrasound evidence of vasculopathy was not found...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000153302.27299.16
更新日期:2005-02-15 00:00:00
abstract:BACKGROUND:Despite effective antiviral treatment, hundreds of kidneys from deceased donors with hepatitis C virus (HCV) are discarded annually. Little is known about the determinants of willingness to accept HCV-infected kidneys among HCV-negative patients. METHODS:At 2 centers, 189 patients undergoing initial or reev...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002099
更新日期:2018-04-01 00:00:00
abstract::Rabbit antisera to rat thoracic duct lymphocytes were examined by absorption analysis with quantitative radioimmunoassays to determine the tissue distribution of the antigens recognised and the amount of antibody against different specificities. Seven of eight sera were remarkably specific for leucocytes and one serum...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197704000-00009
更新日期:1977-04-01 00:00:00
abstract::Experimental evidence suggests storing lungs inflated with oxygen and with oxidizable substrate improves results of lung transplantation. Glucose is included in the low-potassium-dextran (LPD) solution Perfadex to achieve this goal. The authors hypothesized that other substrates might be more effective. Rat lungs were...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000169129.45433.b6
更新日期:2005-08-15 00:00:00
abstract:BACKGROUND:Ventricular assist devices (VADs) are increasingly being used in pediatric patients to provide long-term cardiac support. One potential complication of VAD therapy is the development of antibodies directed against human leukocyte antigens (HLA). This phenomenon has not been well described with the Berlin Hea...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182925242
更新日期:2013-06-27 00:00:00
abstract:BACKGROUND:Cold ischemia-reperfusion injury is unavoidable during organ transplantation, and prolonged preservation is associated with poorer function recovery. Cardiotrophin-1 (CT-1) is an IL-6 family cytokine with cytoprotective properties. This preclinical study in rats tested whether CT-1 mitigates cold renal ische...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002313
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Preconditioning protects against both liver and lung damage after hepatic ischemia-reperfusion (I/R). Xanthine and xanthine oxidase (XOD) may contribute to the development of hepatic I/R. OBJECTIVE:To evaluate whether preconditioning could modulate the injurious effects of xanthine/XOD on the liver and lung...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200204270-00004
更新日期:2002-04-27 00:00:00