Abstract：
:Our objective was to compare different methods for studying programmed cell death in adherent H460 non-small lung cancer cells of moderate clonogenic radiosensitivity. The major effect of gamma-radiation was found to be the release of cells from the substratum. The different methods gave complementary and unexpected information: a) with the TUNEL method, a few non-apoptotic cells were found in the culture medium; b) with the flow cytometry after propidium iodide labeling, some hypodiploid cells which remained attached to the substratum were apoptotic, as demonstrated by the effect of a caspase inhibitor; c) with the annexin V labeling, the detached cells were demonstrated either necrotic or very late apoptotic; d) the mitochondria transmembrane potential (deltapsim), measurements demonstrated that the mitochondria were implicated in cell death induced by gamma-radiation. These data illustrate the need to use several complementary methods in the study of apoptosis in adherent cells exposed to gamma-radiation.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Broquet P,Rodriguez-Lafrasse C,Alphonse G,Aloy MT,Louisot P,Rousson Rsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
362-9issue
5eissn
0028-2685issn
1338-4317journal_volume
48pub_type
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