Abstract:
:Dipeptidyl peptidase IV (DP IV, CD26), known as an activation marker of T lymphocytes, is a proline-specific protease thought to be involved in the regulation of the immune response. The physiological role of dipeptidyl peptidase IV in the immune system and the molecular events of lymphocyte activation mediated by this enzyme are only partly established. Former results suggested the occurrence of different molecular forms of DP IV in distinct human sources. As yet it has been unknown whether DP IV from human hematopoietic cells also appears in different forms and whether similar structural modifications are involved in functional processes of the regulation of the immune response. Here we describe that lymphocytic DP IV/CD26 occurs in various molecular forms and that some of them are associated with the activation process. In cell lysates of mitogen-activated lymphocytes at least 5 enzymatically active DP IV forms and up to 11 immunoreactive molecular forms of this enzyme with isoelectric points between pH 3.5 and 5.9 were discernible. Corresponding analyses of soluble and membrane cell fractions of human lymphocytes showed significant differences in the staining pattern of molecular DP IV structures. After mitogenic stimulation a special molecular form of DP IV arises in the membrane, which was originated either from the soluble part of the cell (translocation) or represents a new synthesized form. Particularly, changes of molecular DP IV forms after mitogenic stimulation strongly suggest that special forms/epitopes of this enzyme are directly involved in the process of lymphocyte activation and growth. Importantly, different monoclonal DP IV antibodies partly define different molecular forms of DP IV. Moreover, the pattern of immunostaining and enzymatic staining (Gly-Pro-beta-methoxynaphthylamide) also reveals drastic differences. These data strongly suggest a direct relationship between the expression/recognition of special DP IV epitopes and the contradictory functional effects of monoclonal DP IV antibodies found by us and other groups.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Kähne T,Kröning H,Thiel U,Ulmer AJ,Flad HD,Ansorge Sdoi
10.1006/cimm.1996.0134subject
Has Abstractpub_date
1996-05-25 00:00:00pages
63-70issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(96)90134-3journal_volume
170pub_type
杂志文章abstract::We have established murine T-cell clones which respond to allotypic and species-specific determinants found on chicken erythrocytes (cRBC). Their relative antigen specificities were determined by assessing lymphokine production and proliferation in response to syngeneic spleen cells and cRBC obtained from chickens hom...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90406-5
更新日期:1986-02-01 00:00:00
abstract::The in vivo and in vitro accessory cell requirements of class I major histocompatability complex (MHC) antigen-restricted cytotoxic T-lymphocyte (CTL) responses were determined using cell-depletion experiments coupled with active immunizations using ovalbumin (OVA) as the immunogen and saponin adjuvant (QS-21). To par...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1086
更新日期:1994-04-01 00:00:00
abstract::Mice from strains showing either phenotypical expression of Bcg gene (C57BL/6, BALB/c, DBA/1, and (C57BL/6 X DBA/2)/F1, CBA, A/J, DBA/2) were infected intravenously with 10(7) Mycobacterium lepraemurium (MLM). The number of acid-fast bacilli (AFB) within the spleens, the ability of spleen cells to produce in vitro int...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90026-7
更新日期:1985-03-01 00:00:00
abstract::Murine C57BL/6 thymocytes were cultivated together with syngeneic thymus-derived macrophages (TDM phi) for up to 96 hr to determine whether TDM phi participate in thymocyte maturation. The expression level of H-2b and Thy-1.2 antigens served as thymocyte differentiation surface markers as analyzed by flow cytometry. I...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90310-8
更新日期:1991-05-01 00:00:00
abstract::An Affymetrix mouse genome array and differential in-gel electrophoresis (DIGE) techniques were used to investigate the pharmacological mechanisms of a mixture of herbs, designated CTCM, a compound of traditional Chinese medicine, for the treatment of increased permeability in mouse intestinal microvascular endothelia...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.06.004
更新日期:2010-01-01 00:00:00
abstract::Previous studies established that low in vitro temperatures (27 degrees C, termed nonpermissive) suppressed murine primary thymus-dependent (TD), but not primary thymus-independent (TI) or secondary TD, antibody responses. Suppression was rescued by the addition of oleic acid (18:1), recombinant interleukin (rIL)-2 an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1265
更新日期:1993-11-01 00:00:00
abstract::Various anti-Fc gamma RIII (CD16) monoclonal antibodies (mAbs) are shown here to have positive or negative modulatory effects on human NK cells. Thus, 3G8 mAb (IgG1) triggered a dose-dependent augmentation of NK activity in 67% (23/34) of individuals tested, who were designated as responders. All four IgG1 anti-CD16 m...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1113
更新日期:1995-07-01 00:00:00
abstract::Immunocytochemical staining procedures were combined with computer-aided image analysis to quantitate the relative intracellular production of tumor necrosis factor-alpha (TNF) within individual macrophages. Optimal conditions for time and methods for the activation of TNF production, fixation of cells for optimal imm...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90278-y
更新日期:1990-10-15 00:00:00
abstract::A spontaneously arising murine plasmacytoma, HPC-202, derived from a BALB/c.H-2b congenic mouse that lacks any detectable H-2 determinants on its cell surface is described. However, the expression of H-2 determinants is inducible by interferon-gamma. The H-2 negative cell surface phenotype permits the HPC-202 tumor to...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90114-7
更新日期:1990-04-15 00:00:00
abstract::We investigated the role of HLA-DR molecules in T cell stimulation by staphylococcal enterotoxin A (SEA). Previous results with immobilized purified HLA-DR preincubated with peptide showed that peptide-specific T cell clones were able to bind to and proliferate in response to purified HLA-DR/peptide complexes in the a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1255
更新日期:1993-10-15 00:00:00
abstract::In this study a panel of monoclonal antibodies was used to investigate the kinetics of the appearance of activation-linked surface determinants as well as cytoplasmic and nuclear determinants in human T cells following lectin stimulation. Well known activation markers, such as Ia/DR, transferrin receptor, IL-2 recepto...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90197-2
更新日期:1985-02-01 00:00:00
abstract::We systematically investigated the impact of the relative maturation levels of dendritic cells (DCs) on their cell surface phenotype, expression of cytokines and chemokines/chemokine receptors (by DNA array and RNase protection analyses), biological activities, and abilities to induce tumor immunity. Mature DCs expres...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1883
更新日期:2001-11-25 00:00:00
abstract::In view of the eminently important role of interleukin-2 (IL-2) in T-cell responses, and in view of reports about immune stimulatory effects of PGE2, we reinvestigated the question whether PGE2 inhibits IL-2 production. It was found that PGE2 does not inhibit IL-2 production in murine spleen cell cultures after optima...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90180-7
更新日期:1985-01-01 00:00:00
abstract::CD20 is expressed strictly by B-cells and is ubiquitously expressed at high surface densities of malignant human B-cells. This suggests that CD20 may be a tumor target for immunotherapy of B-cell lymphomas. Rituximab, a chimeric monoclonal antibody directed against CD20, has been demonstrated to be an effective treatm...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.05.005
更新日期:2006-02-01 00:00:00
abstract::Monocytes and macrophages link the innate and adaptive immune systems and protect the host from the outside world. In inflammatory disorders their activation leads to tissue damage. Galectins have emerged as central regulators of the immune system. However, if they regulate monocyte/macrophage physiology is still unkn...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.003
更新日期:2011-01-01 00:00:00
abstract::In vivo treatment with anti-IA antibodies has been shown to induce a haplotype-specific inhibition of EAE when the disease was following passive transfer of MBP-sensitized T cells. In order to determine the mechanism by which anti-IA antibody prevents passively transferred EAE, the homing of radiolabeled cells to the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90267-f
更新日期:1991-06-01 00:00:00
abstract::The kinetic and the H-2 requirements for the induction of nucleoside-specific suppression were examined in several strains of mice; specifically, whether adenosine (A)-coupled spleen cells given intravenously suppress the primary response to adenosine-KLH. The adenosine system was chosen because C57Bl/6 mice were orig...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90333-7
更新日期:1983-02-01 00:00:00
abstract::Inhibition of DPP IV has been shown to abrogate the stress-related increase in murine abortions and a concomitant increase in gamma-interferon. The aim of the present study was to investigate a potential impact of the DPP IV inhibitor Isoleucine Cyanopyrrolidide on the expression of surface antigens involved in T-cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00028-5
更新日期:2002-12-01 00:00:00
abstract::We previously showed that DT-5461a, a lipid A analog, has antitumor activity and relatively low toxicity in animals. To clarify its immunostimulatory effect we have studied the cytokine inducibility of DT-5461a and the mechanism involved in the cytokine expression in macrophages. While the activity is lower than that ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1100
更新日期:1994-04-15 00:00:00
abstract::Transformed murine fetal thymocyte cell lines were derived by incubating fetal thymic organ cultures with a v-myc/v-raf-containing retroviral construct in order to model developmental stages within the early triple negative (CD3-CD4-CD8-) thymocyte population. The resulting 10 cell lines had a lymphoid morphology, wer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0170
更新日期:1996-07-10 00:00:00
abstract::The activation by concanavalin A (Con A) of murine lymphocytes derived from aged animals is impaired, as demonstrated by lower IL-2 synthesis, display of fewer IL-2 receptors, and less [3H]thymidine incorporation relative to similarly treated cells from young animals. The ability of the phorbol ester, phorbol 12-myris...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90250-x
更新日期:1988-06-01 00:00:00
abstract::Incubation of C57BL/6 thymus-derived macrophages (TDM phi) with syngeneic thymocytes resulted in binding of thymocytes to macrophages and rosette formation. Up to 60% of the TDM phi formed rosettes with thymocytes after 6 hr of interaction at 4 degrees C. Rosette formation of the immature PNA+ thymocyte fraction was u...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90117-7
更新日期:1988-12-01 00:00:00
abstract::Older humans and experimental animals have been repeatedly found to have higher titers of autoantibodies than do younger individuals despite the impaired responses of older individuals to foreign antigens. The studies reported here were designed to examine the relationship between these two age-related changes in anti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90294-l
更新日期:1991-07-01 00:00:00
abstract::Modification of cytokine production by gender hormones has been postulated to affect disease susceptibility and outcome. Here we investigate the effect of gender and the menstrual cycle on production of cytokines. Mononuclear cells were isolated every week for 10 consecutive weeks from healthy pre-menopausal women and...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.04.013
更新日期:2008-02-01 00:00:00
abstract::In this study we assessed the viability of cultured human umbilical vein endothelial cells (HUVE) treated with bacterial lipopolysaccharide (LPS), recombinant human interleukin-1 (rhIL-1), or recombinant human tumor necrosis factor-alpha (rhTNF-alpha) during inhibition of RNA or protein synthesis. Cytotoxicity was det...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90222-0
更新日期:1989-03-01 00:00:00
abstract::Mature thymocytes are usually defined and separated from other less mature thymocytes on the basis of their mutually exclusive expression of either CD4 or CD8. However, such murine "single positives" include a subpopulation of immature cells with properties resembling CD4- CD8- thymocytes or CD4+ CD8+ cortical blasts....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90042-1
更新日期:1988-05-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is the etiological agent most frequently associated with bacterial exacerbations of chronic obstructive pulmonary disease (COPD). The present work shows that NTHi strains induced in primary normal human bronchial epithelial cells (NHBE) a cytokine/chemokine response in which C...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.09.008
更新日期:2010-01-01 00:00:00
abstract::In the present study, we characterized in monocytes the rise in [Ca(2+)](i) evoked by monoclonal antibodies (mAbs) to aminopeptidase N (APN)/CD13, showing a two-phase calcium increase with a small-belled [Ca(2+)](i) rise due to the release of calcium from intracellular stores and a more sustained plateau due to the in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1629
更新日期:2000-04-10 00:00:00
abstract::Evidence is presented that rat serosal mast cells and cells of the rat basophilic leukemia line, RBL-1, each contain a calcium-activated, phospholipid-dependent protein kinase. The enzymes are very similar in their activation requirements to the calcium-dependent enzymes termed protein kinase Cs in brain. The enzyme i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90098-5
更新日期:1986-12-01 00:00:00
abstract::Transforming growth factor beta1 (TGFbeta1) is a potent negative immunoregulatory molecule. We have previously shown that the autoimmune-mediated weaning-age lethality of Tgfb1-/- mice is reversed upon genetic combination with Scid or Rag null alleles. Here, we show that elimination of T but not B cells is sufficient ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.02.004
更新日期:2004-11-01 00:00:00