Abstract:
BACKGROUND/AIMS:The liver is the main source of circulating insulin-like growth factor binding proteins. In man, the cellular origin of insulin-like growth factor binding proteins has remained obscure. METHODS:Human hepatocytes isolated from surgical specimens were purified and cultured using a collagen gel immobilization technique. Gene expression of individual insulin-like growth factor binding proteins and of the acid-labile subunit of the insulin-like growth factor binding proteins by Western ligand blotting and immunoblot analysis. Neutral size chromatography of medium samples was used to detect insulin-like growth factors binding protein complexes. RESULTS:In cultured hepatocytes transcripts for insulin-like growth factor binding protein-1, -2, -3, -4 and for acid labile subunit could be demonstrated. Ligand blotting revealed the secretion of insulin-like growth factor binding proteins of molecular weights of 24 kD, 30 kD, 34 kD, 43 kD and 46 kD, respectively. Using polyclonal antisera, these proteins were identified as insulin-like growth factor binding protein-1, -2 and the insulin-like growth factor binding protein-3 doublet. Neural size chromatography of culture supernatants showed the presence of an insulin-like growth factor binding protein complex of approximately 40 kD, but absence of the high molecular weight ternary complex of 150 kD. CONCLUSIONS:It is concluded that in man parenchymal liver cells have to be regarded as a source of acid-labile subunit and of circulating insulin-like growth factor binding proteins including insulin-like growth factor binding protein-3.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Scharf JG,Schmidt-Sandte W,Pahernik SA,Koebe HG,Hartmann Hdoi
10.1016/0168-8278(95)80201-0subject
Has Abstractpub_date
1995-10-01 00:00:00pages
424-30issue
4eissn
0168-8278issn
1600-0641pii
0168-8278(95)80201-0journal_volume
23pub_type
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更新日期:2011-07-01 00:00:00
abstract:BACKGROUND/AIMS:Bacillus Calmette Guerin (BCG) infection causes hepatic injury following granuloma formation and secretion of cytokines which render mice highly sensitive to endotoxin-mediated hepatotoxicity. This work investigates the role of inducible nitric oxide synthase (iNOS) in liver damage induced by BCG and en...
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更新日期:1999-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
abstract:BACKGROUND/AIMS:Rats with long-term cholestasis have reduced ketosis of unknown origin. METHODS:Fatty acid metabolism was studied in starved rats with biliary obstruction for 4 weeks (bile duct ligated rats = BDL rats), and 3, 7, 14, 28 and 84 days after reversal of biliary obstruction by Roux-en-Y anastomosis (RY rat...
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更新日期:2002-11-01 00:00:00
abstract:BACKGROUND/AIMS:We evaluated the test performance profile (TPP) of blood tests of liver fibrosis. METHODS:Three hundred and fifty-six patients with C chronic hepatitis were included in two centers. Metavir staging of liver specimens by two independent pathologists and the following tests were evaluated: Fibrotest (FT)...
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doi:10.1016/j.jhep.2006.09.020
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND & AIMS:Recently, we have shown that micro-metastases, in the hypoxic transition zone surrounding lesions generated by radiofrequency ablation (RFA), display strongly accelerated outgrowth. CD95 is best known for its ability to induce apoptosis but can also promote tumorigenesis in apoptosis-resistant tumor c...
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pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is associated with tremendous morbidity and mortality due to liver complications. HCV infection is also associated with many extrahepatic manifestations including cardiovascular diseases, glucose metabolism impairment, cryoglobulinemia vasculitis, B cell non-Hodgkin lymphoma and chron...
journal_title:Journal of hepatology
pub_type: 杂志文章,评审
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journal_title:Journal of hepatology
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更新日期:2007-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 杂志文章,评审
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更新日期:2016-05-01 00:00:00
abstract::Secondary biliary cirrhosis in the rat is an attractive model since unlike other models it does not rely on exogenous toxic compounds to induce cirrhosis. However, because little is known about the microcirculatory abnormalities of this model, this study investigated hemodynamics in rats with predefined functional imp...
journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/s0168-8278(05)80031-5
更新日期:1993-02-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/j.jhep.2012.05.024
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND & AIMS:Non-alcoholic steatohepatitis (NASH) is a leading cause of chronic liver disease in Western countries. It is unclear how infiltrating leukocytes affect NASH-development. Our study aims to investigate the role of the homing/receptor, pair mucosal addressin cell adhesion molecule-1 (MAdCAM-1)/β7-Integri...
journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/j.jhep.2017.02.001
更新日期:2017-06-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/s0168-8278(87)80546-9
更新日期:1987-06-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/s0168-8278(99)80214-1
更新日期:1999-08-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jhep.2015.10.005
更新日期:2016-02-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/s0168-8278(05)80447-7
更新日期:1993-01-01 00:00:00
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更新日期:2016-12-01 00:00:00
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更新日期:1986-01-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/s0168-8278(97)80418-7
更新日期:1997-03-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/j.jhep.2012.09.010
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND/AIMS:Selective cyclooxygenase (COX)-2 inhibitors do not adversely affect renal function in experimental cirrhosis. In the current study, we investigated the molecular mechanisms underlying the effects of the selective COX-2 inhibitor, celecoxib, and assessed the influence of albumin on its actions. METHODS:...
journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/j.jhep.2004.09.011
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND & AIMS:Aceruloplasminemia is a rare autosomal recessive neurodegenerative disease associated with brain and liver iron accumulation which typically presents with movement disorders, retinal degeneration, and diabetes mellitus. Ceruloplasmin is a multi-copper ferroxidase that is secreted into plasma and facil...
journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/j.jhep.2010.04.039
更新日期:2010-12-01 00:00:00
abstract::This study evaluates systemic and splanchnic haemodynamics and the effect of propranolol in 15 patients with presinusoidal portal hypertension (portal vein obstruction, n = 11; schistosomiasis, n = 4). These patients exhibited a hyperkinetic circulatory syndrome characterized by high cardiac index (4.4 +/- 1.61.min-1....
journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/0168-8278(89)90139-6
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND/AIMS:Interferon therapy has been shown to have beneficial effects in chronic hepatitis C, but the optimal duration of treatment has not been clearly defined. The aims of this study were: (a) to perform a detailed histological comparison of the effects of a 6-month and a 12-month treatment using the Knodell s...
journal_title:Journal of hepatology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1996-12-01 00:00:00
abstract:BACKGROUND/AIMS:The purpose of this study was to compare the epidemiological, biochemical, virological and histological characteristics of patients with chronic hepatitis B and C with those of patients suffering from chronic hepatitis C alone. METHODS:Twenty-three patients with chronic hepatitis C, who were anti-HCV p...
journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/s0168-8278(98)80198-0
更新日期:1998-01-01 00:00:00
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journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/s0168-8278(97)80091-8
更新日期:1997-10-01 00:00:00
abstract:BACKGROUND/AIMS:Little is known about genuine roles of androgens and their receptor in hepatocellular carcinoma. METHODS:In the present study, two sublines derived from a human hepatocellular carcinoma cell line KYN-1 were used: KYN-1/SM10 with androgen receptor and KYN-1/SM2 without androgen receptor. RESULTS:The bi...
journal_title:Journal of hepatology
pub_type: 杂志文章
doi:10.1016/0168-8278(95)80282-7
更新日期:1995-03-01 00:00:00
abstract::Reactivation of Hedgehog (Hh), a morphogenic signaling pathway that controls progenitor cell fate and tissue construction during embryogenesis occurs during many types of liver injury in adult. The net effects of activating the Hedgehog pathway include expansion of liver progenitor populations to promote liver regener...
journal_title:Journal of hepatology
pub_type: 杂志文章,评审
doi:10.1016/j.jhep.2010.10.003
更新日期:2011-02-01 00:00:00