[Classic and molecular methodologies for the identification of Enterococcus species].

Abstract:

BACKGROUND:Enterococcus is a bacterial genus with low virulence. However, in the last years, the importance of some enterococcus species as nosocomial pathogens has increased, specially due to their resistance to some antimicrobial. AIM:To identify enterococcus strains using classical biochemical techniques and genomic amplification with Polymerase Chain Reaction (PCR). MATERIAL AND METHODS:Three hundred and five enterococcus strains, isolated between 1996 and 1999, from different clinical specimens in hospitals and other centers of the VIIIth Region of Chile, were studied. The isolates were identified, to the species level, according to the scheme proposed by Carvalho et al. Identification of some strains was confirmed by PCR. RESULTS:Eighty nine percent of isolates were identified as E foecalis, 10.2% as E foecium and 3.3% as other species. CONCLUSIONS:PCR is a fast and promising technique, useful in the identification of Enterococcus species.

journal_name

Rev Med Chil

journal_title

Revista medica de Chile

authors

Sepúlveda M,Bello H,Ruiz M,Hormazábal J,Domínguez M,González G,Mella S,Zemelman R

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

45-9

issue

1

eissn

0034-9887

issn

0717-6163

journal_volume

130

pub_type

杂志文章
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    doi:

    authors: Rodríguez MI,Estay R,Soto JR,Wolff C,Plubins L,Child R,Armas R

    更新日期:1993-02-01 00:00:00

  • [False negative spect parathyroid scintigraphy with sestamibi in patients with primary hyperparathyroidism].

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    journal_title:Revista medica de Chile

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    authors: Majlis S,Horvath E,Castro L,Martínez V

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  • [Association between physical fitness parameters and health related quality of life in Chilean community-dwelling older adults].

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  • [Response rates to first-line treatment in eligible patients to autologous stem transplantation in Chile].

    abstract::Background The treatment of choice of newly diagnosed multiple myeloma (NDMM) is an induction with proteasome inhibitors followed autologous stem cell transplantation (HSCT). Since 2013, the treatment of these patients in the public system is based on CTD (cyclophosphamide, thalidomide, and dexamethasone). Aim To eval...

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    pub_type: 杂志文章,多中心研究

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    authors: Peña C,Rojas-Vallejos J,Espinoza M,Donoso J,Soto P,Cardemil D,Aranda S,Contreras C,Vergara CG,LA Rocca G,Osorio R,López-Vidal H,Chandía M,Rojas C

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  • [Soft tissue sarcoma. Combined treatment of drug therapy, radiotherapy and conservative surgery].

    abstract::The preliminary results of a prospective and cooperative protocol for the treatment of soft tissue sarcomas of trunk and extremities, stages IB-IIIB are presented. Thirty one patients (17 males, 22 with a tumor larger than 5 cm) aged 16 to 68 years, were treated with one dose of epirubicin 70 mg/m2 i.v. and radiothera...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: San Martín S,Pérez R,Sepúlveda M,Rodríguez M,Durán B,Trujillo C,Arraztoa J

    更新日期:1993-02-01 00:00:00

  • [Serotypes and antimicrobial susceptibility of Streptococcus agalactiae].

    abstract:BACKGROUND:Streptococcus agalactiae or group B streptococcus, GBS, is the leading cause of neonatal and maternal infections and an opportunistic pathogen in adults with underlying disease. In the last decade, a dramatic increase in the resistance of this microorganism to erythromycin and clindamycin has been observed. ...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:10.4067/s0034-98872004000500003

    authors: Martínez MA,Ovalle A,Durán C,Reid I,Urriola G,Garay B,Cifuentes M

    更新日期:2004-05-01 00:00:00

  • [Determinant factors of excess of weight in school children: a multilevel study].

    abstract:BACKGROUND:Rates of obesity reach high levels in Chile, with geographic, social and school variations. AIM:To identify factors at two levels associated with excessive weight in school children: child-family characteristics and school-neighborhood. MATERIAL AND METHODS:Using a cross-sectional and multi-step design, se...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:/S0034-98872007001200002

    authors: Amigo H,Bustos P,Erazo M,Cumsille P,Silva C

    更新日期:2007-12-01 00:00:00

  • [Vitamin D receptor gene polymorphisms and risk of hip fracture in Chilean elderly women].

    abstract:BACKGROUND:Osteoporotic hip fractures are devastating events in older women. There is a genetic modulation of bone phenotypic parameters including bone density (BMD) and bone fragility fractures. Vitamin D receptor (VDR) gene polymorphisms explain a small part of the genetic influence on BMD, whereas their effect on fr...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:/S0034-98872008000400008

    authors: Quevedo L I,Martínez B M,Castillo N M,Rivera F N

    更新日期:2008-04-01 00:00:00

  • [Rapid aspirin desensitization in patients with a history of aspirin hypersensitivity requiring coronary angioplasty. Report of four cases].

    abstract::Aspirin use is necessary after a coronary angioplasty. It should not be used in patients with a history of hypersensitivity. However, rapid desensitization protocols have been reported to allow its use in such patients. One of these protocols consists in the administration of progressive doses of aspirin, from 1 to 10...

    journal_title:Revista medica de Chile

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    authors: Veas P N,Martínez G,Jalil M J,Martínez S A,Castro G P

    更新日期:2013-02-01 00:00:00

  • [Sympathetic denervation of an extremity as a presentation form of apical lung cancer].

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    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Campero M,Verdugo RJ,Stuardo A,Tenhamm E

    更新日期:1995-05-01 00:00:00

  • [Prenatal exposure to androgens as a factor of fetal programming].

    abstract::Both epidemiological and clinical evidence suggest a relationship between the prenatal environment and the risk of developing diseases during adulthood. The first observations about this relationship showed that prenatal growth retardation or stress conditions during fetal life were associated to cardiovascular, metab...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章,评审

    doi:10.4067/s0034-98872006000100015

    authors: Recabarren SE,Sir-Petermann T,Maliqueo M,Lobos A,Rojas-García P

    更新日期:2006-01-01 00:00:00

  • [Integrons and resistance gene cassettes: structure and role against antimicrobials].

    abstract::Bacteria have developed sophisticated and successful genetic mechanisms to evade the action of antimicrobials. Bacterial multiresistance has caused serious problems in the treatment of nosocomial infections. Integrons and gene cassettes are considered the main genetic elements in the evolution of plasmids and transpos...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章,评审

    doi:10.4067/s0034-98872004000500013

    authors: González G,Mella S,Zemelman R,Bello H,Domínguez M

    更新日期:2004-05-01 00:00:00

  • [Recurrent Impetigo Herpetiformis: successfully managed with ciclosporine. Report of one case].

    abstract::Impetigo Herpetiformis is a high-risk gestational skin disease that represents a risk for both the mother and offspring. Its management is based on multisystemic support and maternal steroid therapy. When these measures are insufficient to control the disease, the association of ciclosporine to the treatment has been ...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:10.4067/s0034-98872005000900011

    authors: Valdés E,Núñez T,Pedraza D,Muñoz H

    更新日期:2005-09-01 00:00:00

  • [CD44 (HCAM) expression in subserous gallbladder carcinoma].

    abstract:BACKGROUND:HCAM or CD44 is a multifunctional cell adhesion molecule, related to cell-cell, cell-extracellular matrix interactions and involved in tumor invasion. AIM:To study the importance of CD44 expression in subserous gallbladder carcinoma. MATERIAL AND METHODS:One hundred five samples (93 female) of subserous ga...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Roa I,Villaseca M,Araya J,Roa J,de Aretxabala X,Ibacache G,García M

    更新日期:2001-07-01 00:00:00

  • [Focal hepatic lesions observed by echotomography with special reference to hydatidosis].

    abstract::This article reports the results of 1080 ultrasonographic examinations of the liver in a clinic of the Ninth Region of Chile, using a SSD 620 Aloka equipment. In 61 examinations (5.6%) a focal liver lesion was found. Of these, 9 (14.7% of lesions) appeared as malignant (6 metastasis and 3 hepatomas) and 52 (85.3% of l...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Calderón C

    更新日期:1994-03-01 00:00:00

  • [Reproductive history of Chilean puerperal women hospitalized in a public hospital of the metropolitan region].

    abstract:BACKGROUND:Maternal and infantile morbidity is closely related to the features of women's reproductive history. AIM:To study the reproductive behavior of women hospitalized due to labor or abortion in a public maternity of Metropolitan Santiago. SUBJECTS AND METHODS:One thousand women were interviewed about their rep...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Henríquez R,Vargas N

    更新日期:1996-10-01 00:00:00

  • [Sexual behavior and contraceptive practices among university students].

    abstract::The aim of this work was to study the knowledge, opinions and sexual behaviour of a sample of 464 students from the Universidad Austral de Chile. Results show that 78% of male and 41% of female students have had a sexual intercourse and that 78% of males and 72% of females with an active sexual life use contraceptive ...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Repossi A,Araneda JM,Bustos L,Puente C,Rojas C

    更新日期:1994-01-01 00:00:00

  • [Advertising and communicative attitude: towards an ethics of publicity in medicine].

    abstract::Starting of from the notion that medicine presents a plurality of aims, it is proposed that it should be conceived as a "science of actions" rather than as a "science of objects". Health is not a neutral "object" that could be unambiguously defined and approached from any vantage point solely with the standards of a d...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Stepke FL

    更新日期:1989-11-01 00:00:00

  • [Child physical punishment: opinions of the children from 2 schools].

    abstract::To measure the proportion, reasons and frequency of battering and punishment, we surveyed seventh and eight grade children coming from a low and middle income area, state funded school (n = 84) and from a payed, private and catholic school (n = 98). Of children coming from the payed school, 67% lived with both parents...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Vargas NA,López D,Ciocca P,López C

    更新日期:1994-08-01 00:00:00

  • [Intraoperative monitoring of gastric intramucosal pH].

    abstract:BACKGROUND:Gastric mucosal pH may be a good indicator of splanchnic tissue oxygenation. AIM:To study the effects of abdominal surgical procedures on gastric mucosal pH. PATIENTS AND METHODS:Eighteen patients subjected to abdominal surgery were studied. All patients received general anesthesia and hemodynamic paramete...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Concha M,Añazco R,González A,Becker D,Gajardo A

    更新日期:1996-08-01 00:00:00

  • [Pharmacokinetic comparison of Sinemet and Grifoparkin (levodopa/carbidopa 250/25 mg) in Parkinson s disease: a single dose study].

    abstract:BACKGROUND:There are doubts wether generic medications have the same bioavailability and efficacy compared with the original drugs developed by pharmaceutical companies with research capabilities. AIM:To compare the pharmacokinetics and clinical (motor) responses of Sinemet and Grifoparkin (generic carbidopa/levodopa ...

    journal_title:Revista medica de Chile

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Chaná P,Fierro A,Reyes-Parada M,Sáez-Briones P

    更新日期:2003-06-01 00:00:00

  • [A survey about physicians’ professional satisfaction].

    abstract:BACKGROUND:A change in the social structure of medicine and its impact on clients has occurred in the last decades. AIM:To perform a survey about subjective wellbeing among physicians. MATERIAL AND METHODS:A physician’ professional satisfaction survey consisting in 90 questions, was applied to 580 physicians (7...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:/S0034-98872011001000009

    authors: Bascuñán ML,Horwitz N,Schiattino I,Acuña J,Jiménez JP

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  • [Practice of Internal Medicine in Latin America. Role of the internist].

    abstract::This article explores the causes of the crisis in the role of internists. As in the United States, the progressive specialization of internists lead to a dehumanized, expensive and technical practice of medicine. Aiming to better incomes and prestige, more than 60% of internists practice as specialists. Primary care p...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Varela N

    更新日期:2002-01-01 00:00:00

  • [Primary hyperthyroidism after excision of a Struma ovarii].

    abstract::Struma ovarii is a teratoma composed of thyroid tissue and can be a rare cause of hyperthyroidism. We report a 35 years old woman with a left ovarian mass whose pathology revealed a Struma ovarii. This tumor was partially excised initially to avoid fertility problems and after a successful gestation, the tumor was com...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Sapunar J,Roa JC,Meissner A

    更新日期:2003-07-01 00:00:00

  • [Etiology and biomarkers of systemic inflammation in mild to moderate COPD exacerbations].

    abstract:BACKGROUND:The etiology of acute exacerbations of chronic obstructive pulmonary disease (COPD) is heterogeneous and still under discussion. Inflammation increases during exacerbation of COPD. The identification of inflammatory changes will increase our knowledge and potentially guide therapy. AIM:To identify which inf...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:/S0034-98872012000100002

    authors: Saldías PF,Díaz PO,Dreyse DJ,Gaggero BA,Sandoval AC,Lisboa BC

    更新日期:2012-01-01 00:00:00

  • [Late centronuclear myopathy: autosomal dominant form].

    abstract::We report a family with three generations affected by an autosomal dominant centronuclear palsy. This gene is characterized by ptosis that begins in childhood and a slowly progressive weakness that starts in the second decade of life, involving face, neck and limbs. In this stage, muscle pan associated to exercise or ...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章

    doi:

    authors: Cartier L,Hernández JE

    更新日期:1996-02-01 00:00:00

  • [Alcohol zero tolerance law in Chile: strengths, flaws and shortcomings that should not be overlooked].

    abstract::Chile has recently entered into force Act No. 20.580, which modifies the legal limits of blood alcohol concentration in drivers and increases the penalties for driving under the influence of alcohol, narcotics or psychotropic substances. The aim of this review was to give an account of the strengths of this new law bu...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章,评审

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    authors: González-Wilhelm L,Johnson J,Carnevali R,Ruiz G

    更新日期:2012-07-01 00:00:00

  • [Carcinoma hepatocellular. Description of verified clinical characteristics in a region of Southern Chile].

    abstract:BACKGROUND:Hepatocellular carcinoma is the most frequent primary tumor of the liver. It is associated to chronic liver diseases and has a high prevalence in some regions of Africa and Asia. AIM:To describe the clinical characteristics of patients with hepatocellular carcinoma, admitted to two hospitals of the IX regio...

    journal_title:Revista medica de Chile

    pub_type: 杂志文章,多中心研究

    doi:

    authors: Manterola C,Muñoz S,Araya JC,Calderón C,Barroso MS

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  • [Subclinical atherosclerosis among Chilean subjects classified as having a low cardiovascular Framingham risk].

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    更新日期:2016-01-01 00:00:00