2,2,2-Trifluoroethanol induces helical conformation in an all beta-sheet protein.

abstract：:In addition to a role in translation, AIMP1 is secreted to affect various immune cells, such as macrophages, dendritic cells, B cells, and natural killer cells. However, the direct effects of AIMP1 on T cells have not yet been reported. In this study, we investigated whether AIMP1 could modulate T cell responses direc...

journal_title：Biochemical and biophysical research communications

authors： Kim MS,Lee A,Cho D,Kim TS

更新日期：2019-06-30 00:00:00

abstract：:HIV protease inhibitors (PIs) are often associated with metabolic and cardiovascular complications although they are effective anti-HIV drugs. In this study, we determined whether HIV PI ritonavir could increase endothelial permeability, one of the important mechanisms of vascular lesion formation. Human dermal microv...

journal_title：Biochemical and biophysical research communications

authors： Chen C,Lu XH,Yan S,Chai H,Yao Q

更新日期：2005-09-30 00:00:00

abstract：:Human Molt3 cDNA library was constructed using pcD vector system which permits the expression of cDNA inserts in mammalian cells. Nearly full-length human terminal deoxynucleotidyltransferase (TdT) cDNA was cloned using a fragment of bovine TdT cDNA as a probe. The human TdT cDNA contains an open reading frame of 1,55...

journal_title：Biochemical and biophysical research communications

authors： Koiwai O,Kaneda T,Morishita R

更新日期：1987-04-14 00:00:00

abstract：:A heparan sulfate glycosaminoglycan chain, biotinylated at its reducing-end, was bound to a streptavidin-coated biochip. Surface plasmon resonance spectroscopy showed a low affinity interaction with antithrombin III (ATIII) when it was flowed over a surface containing heparan sulfate. ATIII bound tightly with high aff...

journal_title：Biochemical and biophysical research communications

authors： Hernaiz M,Liu J,Rosenberg RD,Linhardt RJ

更新日期：2000-09-16 00:00:00

abstract：:There have been relatively few studies on the effects of neurotransmitters on the synthesis of prostaglandins in the brain. We report here that acetylcholine is very effective in stimulating prostaglandin synthesis in cerebellar cortex slices incubated in vitro, i.e., 5 microM acetylcholine increased prostaglandin lev...

journal_title：Biochemical and biophysical research communications

authors： Reichman M,Nen W,Hokin LE

更新日期：1987-08-14 00:00:00

abstract：:G13 belongs to the G12-subfamily of heterotrimeric regulatory G-proteins. Employing specific antibodies, we isolated G alpha 13 from bovine brain by a four-step purification protocol combining conventional and affinity chromatography. The use of ethylene glycol as a protective agent influenced the elution properties o...

journal_title：Biochemical and biophysical research communications

authors： Harhammer R,Nürnberg B,Spicher K,Schultz G

更新日期：1994-10-28 00:00:00

abstract：:Nikkomycins act as a competitive inhibitor of chitin synthetase and display potent activities against phytopathogenic and human pathogenic fungi. sanT is located in the gene cluster of nikkomycin biosynthesis in Streptomyces ansochromogenes. Sequence analysis revealed that the deduced product of sanT has an unusual do...

journal_title：Biochemical and biophysical research communications

authors： Jia L,Tian Y,Tan H

更新日期：2007-11-03 00:00:00

abstract：:The PKC alpha, beta, delta, epsilon and zeta isoenzyme changes in the rat kidney cortex have been investigated after temporary occlusion of the renal artery, followed by reperfusion for up to 4 h. Increased PKC alpha, delta and epsilon immunoreactivity and PKC alpha activity occurred within 60 min of postischemic refl...

journal_title：Biochemical and biophysical research communications

authors： La Porta CA,Comolli R

更新日期：1993-03-31 00:00:00

abstract：:Tubulointerstitial nephritis antigen (TIN-ag) is an extracellular matrix basement protein which was originally identified as a target antigen involved in anti-tubular basement membrane (TBM) antibody-mediated interstitial nephritis (TIN). Further investigations elucidated that TIN-ag plays a role in renal tubulogenesi...

journal_title：Biochemical and biophysical research communications

authors： Ikeda M,Takemura T,Hino S,Yoshioka K

更新日期：2000-02-05 00:00:00

abstract：:The rat Bone/Liver/kidney/Placenta Alkaline Phosphatase (ALP) is transcribed from two alternative promoters spaced over 25 kb apart, resulting in two variant transcripts that are identical in their coding sequence. We investigated the steady-state levels of the two variant transcripts in various rat tissues and cell l...

journal_title：Biochemical and biophysical research communications

authors： Zernik J,Kream B,Twarog K

更新日期：1991-05-15 00:00:00

abstract：:Islet transplantation corrects chronic hyperglycemia by augmentation of insulin supply from the graft tissue, but the role of endogenous beta-cells after transplantation is not clear. In the present study, we examined endogenous beta-cell function after glucose homeostasis had been reestablished by islet graft in stre...

journal_title：Biochemical and biophysical research communications

authors： Hamamoto Y,Tsuura Y,Fujimoto S,Nagata M,Takeda T,Mukai E,Fujita J,Yamada Y,Seino Y

更新日期：2001-09-14 00:00:00

abstract：:Locally applied radiation to the tumor is reported to stimulate systemic immune response. During radiotherapy to the abdominal cancer, spleen often receives certain dose, though as an important immune organ, little is known about the impact of splenic irradiation (SI) on systemic immune and local tumor control. Throug...

journal_title：Biochemical and biophysical research communications

authors： Chen HY,Xie HY,Liu XX,Li LF,Bai YR,Gao JX

更新日期：2019-02-26 00:00:00

abstract：:Reduction of the four Fe centers is not required to initiate the reaction of the Halomonas halodenitrificans nitric oxide reductase (NOR) based on the facts that NOR in the form that ferric heme b(3) and non-heme iron (Fe(B)) are not bridged and/or the interaction between them is weakened and reversibly binds NO molec...

journal_title：Biochemical and biophysical research communications

authors： Sakurai T,Nakashima S,Kataoka K,Seo D,Sakurai N

更新日期：2005-07-29 00:00:00

abstract：:Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above system, gave significan...

journal_title：Biochemical and biophysical research communications

authors： Roy R,Nasrin N,Ahmad MF,Gupta NK

更新日期：1984-08-16 00:00:00

abstract：:Protein aggregate/inclusion is one of hallmarks for neurodegenerative disorders including amyotrophic lateral sclerosis (ALS). FUS/TLS, one of causative genes for familial ALS, encodes a multifunctional DNA/RNA binding protein predominantly localized in the nucleus. C-terminal mutations in FUS/TLS cause the retention ...

journal_title：Biochemical and biophysical research communications

authors： Takanashi K,Yamaguchi A

更新日期：2014-09-26 00:00:00

abstract：:Mutations in the GDD1 gene cause gnathodiaphyseal dysplasia, a rare human skeletal syndrome with autosomal dominant inheritance. The biochemical function(s) of GDD1 protein and the molecular pathophysiology of GDD1 mutations leading to GDD have not yet been elucidated. In this study, we characterized the complete cDNA...

journal_title：Biochemical and biophysical research communications

authors： Tsutsumi S,Inoue H,Sakamoto Y,Mizuta K,Kamata N,Itakura M

更新日期：2005-06-17 00:00:00

abstract：:Thioredoxin (TRX) is one of major components of thiol reducing systems. To investigate the molecular mechanism of TRX function in the lung tissue, we screened a human lung epithelial cell cDNA library for TRX-binding protein by yeast two-hybrid systems. We isolated a plasmid containing C-propeptide region of human pro...

journal_title：Biochemical and biophysical research communications

authors： Matsumoto K,Masutani H,Nishiyama A,Hashimoto S,Gon Y,Horie T,Yodoi J

更新日期：2002-07-19 00:00:00

abstract：INTRODUCTION:Chemical intervention of autophagy has been investigated in clinical trials for various age-related conditions such as sarcopenia and neurodegeneration. However, at present, no autophagy inducer has been established as a disease-modifying agent against neurodegenerative diseases. METHODS:We screened a lib...

journal_title：Biochemical and biophysical research communications

authors： Hirano K,Fujimaki M,Sasazawa Y,Yamaguchi A,Ishikawa KI,Miyamoto K,Souma S,Furuya N,Imamichi Y,Yamada D,Saya H,Akamatsu W,Saiki S,Hattori N

更新日期：2019-10-08 00:00:00

abstract：:Adipogenesis is the process of differentiation from preadipocytes to adipocytes and is orchestrated by various transcription factors, such as the peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAAT-enhancer-binding protein alpha (C/EBPα). Oxidative stress is also a crucial factor in adipogenesis, an...

journal_title：Biochemical and biophysical research communications

authors： Kim BR,Lee GY,Yu H,Maeng HJ,Oh TJ,Kim KM,Moon JH,Lim S,Jang HC,Choi SH

更新日期：2018-03-18 00:00:00

abstract：:Measurements of the cytosolic calcium concentration in single cells of the clonal endocrine pancreatic glucagon-secreting cell line INR1 G9 revealed the existence of spontaneous calcium oscillations in 20-70% of these cells. Inhibition of these spontaneous oscillations by thapsigargin as well as the phospholipase C in...

journal_title：Biochemical and biophysical research communications

authors： Bode HP,Yule DI,Fehmann HC,Göke B,Williams JA

更新日期：1994-11-30 00:00:00

abstract：:In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-typ...

journal_title：Biochemical and biophysical research communications

authors： Ishizaka Y,Kangawa K,Minamino N,Ishii K,Takano S,Eto T,Matsuo H

更新日期：1992-12-15 00:00:00

abstract：:Using fluorescence and UV-vis spectroscopies and mass spectrometry, we demonstrated that the presence of physiological levels of reduced glutathione enhances the binding of Zn(II) to XPAzf, a Cys4 zinc finger peptide derived from the XPA protein, by means of formation of a ternary complex of a general formula ZnXPAzf[...

journal_title：Biochemical and biophysical research communications

authors： Piatek K,Hartwig A,Bal W

更新日期：2009-11-13 00:00:00

abstract：:Bacteria belonging to the Burkholderia cepacia complex (BCC) are important opportunistic pathogens in patients with cystic fibrosis (CF). Since approximately 80% of the CF isolates examined produce exopolysaccharide (EPS), it was hypothesized that this EPS may play a role in the colonization and persistence of these b...

journal_title：Biochemical and biophysical research communications

authors： Moreira LM,Videira PA,Sousa SA,Leitão JH,Cunha MV,Sá-Correia I

更新日期：2003-12-12 00:00:00

abstract：:Four clonal cell lines subcloned from a clonal population of fetal rat calvaria cells show a loss of type I collagen synthesis. Northern blot analysis showed that the level of alpha 1(I) collagen mRNA expression in each of the clonal populations parallels the level of collagen protein expression in each of these cell ...

journal_title：Biochemical and biophysical research communications

authors： Waye MM,Robinson R,Orfanides AG,Aubin JE

更新日期：1989-08-15 00:00:00

abstract：:G proteins play vital roles in cellular responses to external signals. The specificity of G protein-receptor interaction is mediated mostly by the gamma-subunit and the individual members of the gamma-subunit multigene family would hence be expected to each have a particular expression profile. In an experiment design...

journal_title：Biochemical and biophysical research communications

authors： Modarressi MH,Taylor KE,Wolfe J

更新日期：2000-06-07 00:00:00

abstract：:We determined the cDNA sequence for neurocalcin, a novel calcium-binding protein in bovine brain. This clone (pCalN) has 582 nucleotides in the open reading frame including the termination codon TGA, 11 nucleotides of the 5' leader and 1251 nucleotides of the 3' noncoding region. The deduced amino acid sequence reveal...

journal_title：Biochemical and biophysical research communications

authors： Okazaki K,Watanabe M,Ando Y,Hagiwara M,Terasawa M,Hidaka H

更新日期：1992-05-29 00:00:00

abstract：:It has long been assumed that Ca2+ are translocated from the cytosol to the cell nucleus by a long distance to activate transcription machinery buried deep in the nucleoplasm. However, this model has been recently challenged. When HeLa cells were loaded with fluo-3, highly fluorescent spots of approximately 2 microns ...

journal_title：Biochemical and biophysical research communications

authors： Lui PP,Kong SK,Kwok TT,Lee CY

更新日期：1998-06-09 00:00:00

abstract：:TAp63 belongs to the p53-tumour suppressor family and is capable of transactivating a set of target genes to induce cell cycle arrest and apoptosis. We showed that treatment of cancer cells with chemo-therapeutic drugs or the histone deacetylase (HDAC) inhibitor Trichostatin A (TSA) results in induction of TAp63 expre...

journal_title：Biochemical and biophysical research communications

authors： Sayan BS,Yang AL,Conforti F,Bernardini S,Tucci P,Vasa-Nicotera M,Knight RA,Melino G

更新日期：2010-01-22 00:00:00

abstract：:High performance liquid chromatographic (HPLC) analysis showed that the prototype antioxidant ascorbate (vitamin C) inhibits the DNA adducts induced by synthetic estrogen diethylstilbestrol (DES) and the antiestrogen metabolite 4-hydroxytamoxifen (4-OHTam). Treatment of salmon testes DNA with 4-OHTam quinone or 4-OHTa...

journal_title：Biochemical and biophysical research communications

authors： Sharma M,Slocum HK

更新日期：1999-09-07 00:00:00

abstract：:The transcriptional factor hypoxia-inducible factor-1α (HIF-1α) is induced under hypoxia and plays crucial roles in cancer progression and angiogenesis. Protein arginine methyltransferases (PRMTs), 11 isoforms of which have been identified so far, modulates the functions of diverse proteins by catalyzing arginine meth...

journal_title：Biochemical and biophysical research communications

authors： Ju UI,Park JW,Park HS,Kim SJ,Chun YS

更新日期：2015-09-04 00:00:00