Abstract:
:We have identified immunologically the protein kinase C (PKC) isoforms present in rat mesenteric small arteries, defined their distribution between particulate and soluble fractions, and studied their involvement in phorbol ester-induced contraction. Our analysis revealed the presence of the CA(2+)-dependent PKCs (alpha and gamma), Ca(2+)-independent PKCs (delta and epsilon), and the atypical isoform (zeta). PKCbeta could not be detected, whereas PKCgamma is likely to be of neural origin. All isoforms exhibited different distributions. PKCalpha, PKCepsilon, and PKCzeta were found in both particulate and soluble fractions. In contrast, PKCdelta was mainly in the particulate fraction, and PKCgamma was in the soluble fraction. Phorbol esters, which activate PKC and cause smooth muscle contraction, downregulated only the alpha and delta isoforms. This was associated with a parallel loss of contractile response to phorbol ester. The force developed to submaximal concentrations of noradrenaline was decreased after phorbol dibutyrate pretreatment, although the sensitivity and maximal response were unchanged. Phorbol ester pretreatment did not affect the contractile response to vasopressin. The sensitivity to non-receptor-mediated contraction, caused by k+ in the presence of prazosin, was slightly reduced by 4 alpha- and 4 beta-phorbol ester pretreatment. Maximal tension in response to this agonist was not affected. We conclude that PKCalpha and/or PKCdelta is necessary for phorbol ester-mediated contraction but is not essential for noradrenaline-, vasopressin-, or k(+)-induced contraction, demonstrating differences in the mechanisms involved in the contractile response between these agents.
journal_name
Circ Resjournal_title
Circulation researchauthors
Ohanian V,Ohanian J,Shaw L,Scarth S,Parker PJ,Heagerty AMdoi
10.1161/01.res.78.5.806subject
Has Abstractpub_date
1996-05-01 00:00:00pages
806-12issue
5eissn
0009-7330issn
1524-4571journal_volume
78pub_type
杂志文章abstract:RATIONALE:Activin receptor-like kinase-1 (ALK1) is an endothelial transforming growth factor β receptor involved in angiogenesis. ALK1 expression is high in the embryo vasculature, becoming less detectable in the quiescent endothelium of adult stages. However, ALK1 expression becomes rapidly increased after angiogenic ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.112.275586
更新日期:2013-01-04 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.120.316653
更新日期:2020-09-11 00:00:00
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更新日期:1997-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.39.1.8
更新日期:1976-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.70.2.438
更新日期:1992-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.65.1.193
更新日期:1989-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-12-08 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.107.160549
更新日期:2007-10-12 00:00:00
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更新日期:2014-08-15 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.70.2.234
更新日期:1992-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2003-05-16 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.48.2.238
更新日期:1981-02-01 00:00:00
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pub_type: 杂志文章,收录出版
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更新日期:1995-07-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.108.187005
更新日期:2009-03-27 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2002-07-26 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.84.8.929
更新日期:1999-04-30 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.108.192443
更新日期:2009-07-02 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.0000032490.04207.bd
更新日期:2002-09-06 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
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journal_title:Circulation research
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更新日期:2015-04-10 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-06-08 00:00:00
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pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
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更新日期:2020-02-28 00:00:00
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pub_type: 杂志文章
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更新日期:2015-02-27 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.78.4.627
更新日期:1996-04-01 00:00:00
abstract::An extensive investigation of the cardiac actions of phorbol esters and the potential role of the Na(+)-H+ exchanger in those actions was carried out using isolated rat hearts. Sixty minutes of perfusion with 10(-9) M phorbol 12-myristate 13-acetate (PMA) or 10(-8) M phorbol 12,13-dibutyrate (PDBu) produced marked car...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.69.4.1114
更新日期:1991-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.56.5.728
更新日期:1985-05-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.62.2.286
更新日期:1988-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.109.195834
更新日期:2010-01-08 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.108.185843
更新日期:2009-01-30 00:00:00