Abstract:
OBJECTIVE:Multiple types of voltage-activated Ca(2+) channels (T, L, N, P, Q and R type) coordinate a variety of Ca(2+)-dependent processes in neurons and neuroendocrine cells. In insulinoma cell lines as well as in endocrine tissues, the non-L-type alpha1E (Ca(v)2.3) subunit is expressed as the tissue-specific splice variant alpha1Ee. DESIGN AND METHODS:To understand the functional role of alpha1E-containing Ca(2+) channels, antisense alpha1E mRNA was overexpressed in INS-1 cells by stable transfection of an antisense alpha1E cassette cDNA. As controls, either a sense alpha1E cassette or a control vector containing enhanced green fluorescent protein as an unrelated gene was stably transfected. The overexpression of each transfected cassette cDNA was recorded by RT-PCR. RESULTS:In three independent antisense alpha1E INS-1 clones, the glucose-induced insulin release was significantly reduced as compared with wild-type INS-1 cells and with a sense alpha1E INS-1 clone. However, in the antisense INS-1 clones, the KCl-induced insulin release was less impaired by overexpressing the antisense alpha1E cassette than the glucose-induced insulin release, leading to the assumption that glucose (15 mmol/l) and KCl (25 mmol/l) finally depolarize the membrane potential to a different extent. CONCLUSION:alpha1E is involved in glucose-induced insulin secretion probably by influencing the excitability of INS-1 cells.
journal_name
Eur J Endocrinoljournal_title
European journal of endocrinologyauthors
Pereverzev A,Vajna R,Pfitzer G,Hescheler J,Klöckner U,Schneider Tdoi
10.1530/eje.0.1460881subject
Has Abstractpub_date
2002-06-01 00:00:00pages
881-9issue
6eissn
0804-4643issn
1479-683Xpii
146881journal_volume
146pub_type
杂志文章abstract::This study was designed to ascertain the long-term safety and efficacy profile of the somatostatin analogue octreotide as treatment of refractory acromegaly. Eight patients (aged 21-62 years) with persistent growth hormone (GH) elevation (duration 1-15 years) despite previous therapy were studied. Octreotide was given...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章
doi:10.1530/eje.0.1320429
更新日期:1995-04-01 00:00:00
abstract:OBJECTIVE:Chronic exposure to elevated glucocorticoid (GC) concentrations induces detrimental effects in several tissues. In the skin, GCs provoke intense alterations on various parameters of the physiology of fibroblasts, cumulatively leading to skin atrophy and impaired wound healing. As there are concerns that GCs m...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.1.01913
更新日期:2005-06-01 00:00:00
abstract:AIM:To characterize the clinical phenotype of type 2 diabetes mellitus (T2DM) with respect to age, gender, and BMI. METHOD:Anonymized data of 120,183 people with T2DM from the German/Austrian multicenter Diabetes Patienten Verlaufsdokumentation database were analyzed based on chronological age or age at diagnosis (0-1...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-12-0143
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVE:Dehydroepiandrosterone (DHEA) is a widely studied steroid hormone with multi-functional properties. Reports suggest that some of the many activities of DHEA are due to its protective effect against lipid peroxidation. Nevertheless, the antioxidant properties of DHEA are still the subject of debate. The aim wa...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1410035
更新日期:1999-07-01 00:00:00
abstract:OBJECTIVE:ATL1103 is a second-generation antisense oligomer targeting the human growth hormone (GH) receptor. This phase 2 randomised, open-label, parallel-group study assessed the potential of ATL1103 as a treatment for acromegaly. DESIGN:Twenty-six patients with active acromegaly (IGF-I >130% upper limit of normal) ...
journal_title:European journal of endocrinology
pub_type: 杂志文章,随机对照试验
doi:10.1530/EJE-18-0138
更新日期:2018-08-01 00:00:00
abstract::Objective To investigate how weight loss by different diets impacts postprandial levels of glucagon-like peptide 1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP) and glucagon. Methods In this single-centre, parallel group 2-year trial, 70 healthy postmenopausal obese women were randomised to the Paleolith...
journal_title:European journal of endocrinology
pub_type: 杂志文章,随机对照试验
doi:10.1530/EJE-19-0082
更新日期:2019-06-01 00:00:00
abstract::Over the last 5 years, new actors involved in the pathogenesis of combined pituitary hormone deficiency in humans have been reported: they included a member of the immunoglobulin superfamily glycoprotein and ciliary G protein-coupled receptors, as well as new transcription factors and signalling molecules. New modes o...
journal_title:European journal of endocrinology
pub_type: 杂志文章,评审
doi:10.1530/EJE-15-1095
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:Adaptive alterations in maternal physiology cause changes in thyroid hormone levels throughout pregnancy, and precise biochemical evaluation is thus highly dependent on gestation-specific reference intervals and expected intra-individual variation. OBJECTIVE:The aim of the study was the assessment of the in...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-09-0579
更新日期:2009-12-01 00:00:00
abstract:BACKGROUND:Sleep behavior is changing toward shorter sleep duration and a later chronotype. It results in a sleep debt that is acquitted on work-free days, inducing a small but recurrent sleep misalignment each week, referred to as "social jetlag". These sleep habits could affect health through misalignment with circad...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-16-0188
更新日期:2016-11-01 00:00:00
abstract::Signalling of receptor activator of nuclear factor-κB (RANK) ligand (RANKL) through RANK is a critical pathway to regulate the differentiation and activity of osteoclasts and, hence, a master regulator of bone resorption. Increased RANKL activity has been demonstrated in diseases characterised by excessive bone loss s...
journal_title:European journal of endocrinology
pub_type: 杂志文章,评审
doi:10.1530/EJE-11-0454
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVE:The purpose of this meta-analysis was to examine the evidence for the effectiveness of aerobic exercise interventions on reducing insulin resistance markers in obese children and/or adolescents. A secondary outcome was change in percentage of body fat. METHODS:A computerized search was made from seven databa...
journal_title:European journal of endocrinology
pub_type: 杂志文章,meta分析
doi:10.1530/EJE-14-0291
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:Glucocorticoid-induced osteoporosis is the most frequent cause of secondary osteoporosis. Nevertheless, limited data are available on bone status in patients with endogenous cortisol excess. This study is aimed at investigating the role of sex steroids and severity of hypercortisolism on bone mineral density ...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-07-0137
更新日期:2007-09-01 00:00:00
abstract::Serum thyrotropin (TSH) concentration circadian rhythm is abolished in many endocrine and non-endocrine diseases. In the present study we have measured serum TSH concentration over 24 h every 2 h in second and third trimester pregnant women. During the 24-h period, serum free thyroxine and free triiodothyronine concen...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1330071
更新日期:1995-07-01 00:00:00
abstract::We examined excretion of urinary insulin-like growth factors I and II (IGF-I and IGF-II) and their major binding protein IGFBP-3 in comparison to their respective serum concentration in nine healthy female volunteers (median age 25 years, range 22-27) under baseline conditions and after stimulation with recombinant hu...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章
doi:10.1530/eje.0.1320433
更新日期:1995-04-01 00:00:00
abstract:INTRODUCTION:The fetus is most vulnerable to severe iodine deficiency and hypothyroidism during pregnancy. The effects of mild iodine deficiency and subclinical hypothyroidism are poorly known. The present study assesses the association between thyroid hormones (TH)s and urinary iodine concentration (UIC) in healthy pr...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-08-0716
更新日期:2009-03-01 00:00:00
abstract::Klinefelter syndrome, 47,XXY (KS), is the most frequent sex chromosome aberration in males, affecting 1 in 660 newborn boys. The syndrome is characterized by testicular destruction with extensive fibrosis and hyalinization of the seminiferous tubules resulting in small testes, hypergonadotropic hypogonadism, and azoos...
journal_title:European journal of endocrinology
pub_type: 杂志文章,评审
doi:10.1530/EJE-12-0934
更新日期:2013-03-15 00:00:00
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journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-19-0658
更新日期:2019-12-01 00:00:00
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journal_title:European journal of endocrinology
pub_type: 杂志文章,多中心研究,评审
doi:10.1530/EJE-13-0503
更新日期:2013-11-22 00:00:00
abstract:OBJECTIVE:A sex difference in fetal and neonatal pituitary-gonadal function has been well documented. The aim of the following study was to determine sex differences and patterns of basal LH/FSH in the neonatal period. DESIGN:Peripheral venous blood was obtained from 164 healthy full term newborns (91 males, 73 female...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1430213
更新日期:2000-08-01 00:00:00
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journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-17-0369
更新日期:2017-10-01 00:00:00
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journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1470333
更新日期:2002-09-01 00:00:00
abstract::Glucose transporter-1 (GLUT1) has been found in high abundance in human placentas. The purpose of this study was to analyze the changes in the level of GLUT1 during pregnancy. We have analyzed the gestational changes in human placental GLUT1 by [3H]cytochalasin B binding assay, immunoblot analysis and Northern blot an...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1320206
更新日期:1995-02-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of long-term GH in GH-deficient adults, as predicted by IGF-I levels. METHODS:Patients received GH, 5 microg/kg per day for 1 Month and 10 microg/kg per day for another 12-30 Months. Changes in body composition, cardiac structure/function, serum lipids and quality of life were meas...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1530/eje.0.1490499
更新日期:2003-12-01 00:00:00
abstract:OBJECTIVE:In view of their different actions on thyroid hormone receptor (TR) isoforms we set out to investigate whether amiodarone (AM) and dronedarone (Dron) have different and/or component-specific effects on cardiac gene expression. DESIGN:Rats were treated with AM or Dron and the expression of TRalpha 1, TRalpha ...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-07-0017
更新日期:2007-06-01 00:00:00
abstract:INTRODUCTION:Activating mutations of the BRAF oncogene are frequently detected in papillary thyroid carcinoma (PTC) and have been associated with a worse prognosis. The amino acid substitution V600E accounts for 90% of all oncogenic BRAF mutations and is typically detected in classic PTCs, whereas other less frequent B...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-08-0239
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVE:Apelin is a novel adipokine acting on APJ receptor, regulated by insulin and tumor necrosis factor-alpha (TNF-alpha) in adipose tissue (AT). Plasma apelin levels are increased in obese hyperinsulinemic subjects. The aim was to investigate whether the hypocaloric diet associated with weight loss modifies the e...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章
doi:10.1530/EJE-08-0039
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:Disturbed renal function may play an important role in the clinico-pathological presentation of primary hyperparathyroidism (pHPT). We studied the influence of renal function on the clinico-pathological characteristics of 141 patients (123 women and 18 men) with surgically proven pHPT. METHODS:The 141 patien...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1480597
更新日期:2003-06-01 00:00:00
abstract:CONTEXT:Measurements of plasma methoxytyramine, the O-methylated dopamine metabolite, are useful for detecting rare dopamine-producing pheochromocytomas and paragangliomas (PPGLs) and head and neck paragangliomas (HNPGLs), but utility for screening beyond that achieved using standard measurements of normetanephrine and...
journal_title:European journal of endocrinology
pub_type: 杂志文章,多中心研究
doi:10.1530/EJE-17-0077
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:It has been suggested that adiponectin regulates plasma free fatty acid (FFA) clearance by stimulating FFA uptake and/or oxidation in muscle. We aimed to determine changes in plasma adiponectin concentration and adiponectin receptor 1 and 2 mRNA expression in skeletal muscle during and after prolonged exercis...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章
doi:10.1530/eje.1.01872
更新日期:2005-03-01 00:00:00
abstract:OBJECTIVE:Biallelic TSHR mutations cause congenital hypothyroidism (CH). Serum TSH levels of monoallelic mutation carriers range from normal to mildly elevated, and thus the size of its effect remains unclear. The objectives were to examine the association between monoallelic TSHR mutations and positivity at newborn sc...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-16-1049
更新日期:2018-02-01 00:00:00