Abstract:
:The pathogenesis of thrombotic thrombocytopenic purpura (TTP) is obscure. It is manifested classically by platelet thrombi and localized microvascular endothelial cell (EC) proliferation, in the absence of an inflammatory response. It is statistically associated with human retroviral disease, but pathological studies of TTP lesions have been unable to establish whether perturbation of the endothelium is a primary or secondary event, irrespective of the presence of retroviral infection. We document that plasma from all of four acute TTP patients, with or without human immunodeficiency virus infection, can induce apoptosis in cultured ECs of microvascular but not large vessel origin. This process was documented by three different methods, (1) laser-illuminated light scatter, (2) quantitation of the pre-G1 Ao peak on DNA histograms and direct visualization of chromatin fragmentation by acridine orange and 4'6-diamidino-2-phenylindole staining, and (3) agarose gel electrophoresis of low molecular weight cellular DNA. Apoptosis was independent of tumor necrosis factor-alpha secretion or the presence of CD36 on microvascular ECs but was linked to the rapid induction of Fas (CD95) on these cells. Soluble anti-Fas antibody, normal plasma depleted of cryoprecipitate, and low concentrations (< or = 0.1 micromol/L) of aurintricarboxylic acid were capable of suppressing TPP plasma-mediated EC apoptosis. In conclusion, microvascular EC apoptosis may be of pathophysiological importance in TTP may be susceptible to interruption by blockade of initiating signals for, or final common enzyme pathways leading to, programmed cell death.
journal_name
Bloodjournal_title
Bloodauthors
Laurence J,Mitra D,Steiner M,Staiano-Coico L,Jaffe Esubject
Has Abstractpub_date
1996-04-15 00:00:00pages
3245-54issue
8eissn
0006-4971issn
1528-0020journal_volume
87pub_type
杂志文章相关文献
BLOOD文献大全abstract::Peripheral blood leukemia cells from patients with acute monoblastic leukemia (AMoL) were tested for killer cell activity against target cells that detected natural killer cell-mediated or monocyte-mediated spontaneous cytotoxicity. The fibrosarcoma cell line Wehi 164, pretreated with actinomycin D to induce susceptib...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Glucocorticoids are keystone drugs in the treatment of childhood acute lymphoblastic leukemia (ALL). To get more insight in signal transduction pathways involved in glucocorticoid-induced apoptosis, Affymetrix U133A GeneChips were used to identify transcriptionally regulated genes on 3 and 8 hours of prednisolone expo...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2006-11-056366
更新日期:2007-05-01 00:00:00
abstract::Immunological control may contribute to achievement of deep molecular response in chronic myeloid leukemia (CML) patients on tyrosine kinase inhibitor (TKI) therapy and may promote treatment-free remission (TFR). We investigated effector and suppressor immune responses in CML patients at diagnosis (n = 21), on TKI (im...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-10-745992
更新日期:2017-03-02 00:00:00
abstract::The primary objective was to describe the prevalence and characteristics of microbiologically defined infections and infection-related mortality (IRM) in 492 children with acute myeloid leukemia enrolled on CCG 2961. Secondary objectives were to determine the relationship between demographic, disease-related, and ther...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2007-05-091942
更新日期:2007-11-15 00:00:00
abstract::The recruitment of phagocytic leukocytes to sites of vessel wall injury plays an important role in thrombus dissolution by proteases elaborated on their adhesion. However, leukocyte adhesion to the fibrin clot can be detrimental at the early stages of wound healing when hemostatic plug integrity is critical for preven...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-05-022764
更新日期:2007-02-15 00:00:00
abstract::Early in life, thymic export establishes the size and the diversity of the human naive T-cell pool. Yet, on puberty thymic activity drastically decreases. Because the overall size of the naive T-cell pool decreases only marginally during ageing, peripheral postthymic expansion of naive T cells has been postulated to a...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2008-02-139154
更新日期:2009-01-22 00:00:00
abstract::Thrombopoietin (Tpo) has proliferative and maturational effects on immature and more committed cells, respectively. We previously reported a role for Tpo as a survival factor in the factor-dependent human cell line M07e by demonstrating that Tpo suppresses apoptosis in the absence of induced proliferation. Wild-type p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::We document findings on c-kit (CD117) expression in 1,937 pediatric and adult de novo acute leukemia cases, diagnosed in five single European centers. All cases were well characterized as to the morphologic, cytochemical, and immunologic features, according to the European Group for the Immunological Classification of...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-07-15 00:00:00
abstract::Antiidiotype (Id) antibodies identify unique determinants within the surface immunoglobulin (Ig) that are present on B-cell tumors. Anti-Ids have been used for diagnosis and therapy of B-cell lymphoma and leukemia. A panel of 29 anti-Id monoclonal antibodies (MoAbs) that recognize shared idiotypes (SIds) on B-cell lym...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::The alternatively spliced and highly conserved EIIIA domain of fibronectin (FN) is included in most FN of the extracellular matrix in embryos. In adults, both extracellular matrix and plasma FN essentially lack EIIIA. In diverse inflammatory situations however, EIIIA is specifically included by regulated RNA splicing....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-09-3363
更新日期:2004-07-01 00:00:00
abstract::Macrophages and dendritic cells play an important role in regulating B-cell responses, including proliferation to antigens such as trinitrophenyl (TNP)-Ficoll and TNP-Brucella abortus. However, the mechanisms and molecule(s) that regulate these processes are relatively undefined. In this report, we show that human mac...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3123
更新日期:2003-06-01 00:00:00
abstract::Human blood contains 2 populations of dendritic cells (DCs): plasmacytoid and myeloid (mDC). mDCs are subdivided into 3 subsets using the surface markers CD16, CD1c, and BDCA-3. Their role as pathogen sentinels and adjuvant targets was tested by phenotypic and functional analysis. We show that mDC subsets are immature...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-08-038422
更新日期:2007-06-15 00:00:00
abstract::Tissue type plasminogen activator (t-PA) is an effective thrombolytic agent in experimental animals. The duration of the thrombolytic effect of infused t-PA is unknown. We compared the duration of the thrombolytic effect of t-PA with streptokinase by measuring the lysis of 125I-fibrin-labeled thrombi in rabbit jugular...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-08-01 00:00:00
abstract::The role of platelets in hemostasis and thrombosis is clearly established; however, the mechanisms by which platelets mediate inflammatory and immune pathways are less well understood. Platelets interact and modulate the function of blood and vascular cells by releasing bioactive molecules. Although the platelet is an...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-12-396440
更新日期:2012-06-28 00:00:00
abstract::The developmental relationship between the blood and endothelial cell (EC) lineages remains unclear. In the extra-embryonic blood islands of birds and mammals, ECs and blood cells are closely intermixed, and blood island precursor cells in the primitive streak express many of the same molecular markers, leading to the...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-03-490045
更新日期:2013-12-05 00:00:00
abstract::We previously reported that RO(+) expression correlated with increased mutation, activation, and selection among human germinal center (GC) B cells. Here, we subdivided human tonsillar B cells, including IgD(-)CD38(+) GC B cells, into different fractions based on RB expression. Although each subset contained RB(+) cel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-03-145979
更新日期:2009-04-23 00:00:00
abstract::Regulated by histone acetyltransferases and deacetylases (HDACs), histone acetylation is a key epigenetic mechanism controlling chromatin structure, DNA accessibility, and gene expression. HDAC inhibitors induce growth arrest, differentiation, and apoptosis of tumor cells and are used as anticancer agents. Here we des...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-05-284711
更新日期:2011-01-27 00:00:00
abstract::Ras-associated autoimmune leukoproliferative disorder (RALD) is a chronic, nonmalignant condition that presents with persistent monocytosis and is often associated with leukocytosis, lymphoproliferation, and autoimmune phenomena. RALD has clinical and laboratory features that overlap with those of juvenile myelomonocy...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2014-11-567917
更新日期:2015-04-30 00:00:00
abstract::CD40 is present on both normal and neoplastic B-lineage cells. CD40 stimulation of normal B cells has been shown to promote normal growth and differentiation, whereas aggressive histology B lymphomas are growth inhibited. The inhibition of neoplastic B-cell growth is believed to occur via activation-induced cell death...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v100.1.217
更新日期:2002-07-01 00:00:00
abstract::CREB-binding protein (CBP) and the closely related adenovirus E1A-associated 300-kD protein (p300) function as coactivators of transcription factors such as CREB, c-Fos, c-Jun, c-Myb, and several nuclear receptors. To study the roles of CBP in embryonic development, we generated CBP homozygous mutant mouse embryos tha...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Hematopoietic stem cell transplantation from unrelated donors is an effective treatment for myeloid malignancies, but its use is usually restricted to young patients without comorbidities. The development of reduced-intensity preparative regimens has allowed the extension of this form of treatment to older and medical...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-03-0855
更新日期:2003-10-15 00:00:00
abstract::The promising activity of BET protein inhibitors (BETi's) is compromised by adaptive or innate resistance in acute myeloid leukemia (AML). Here, modeling of BETi-persister/resistance (BETi-P/R) in human postmyeloproliferative neoplasm (post-MPN) secondary AML (sAML) cells demonstrated accessible and active chromatin i...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019002922
更新日期:2020-04-09 00:00:00
abstract::In this issue of Blood, Prasad et al describe a mouse model with a mutation in the Aα chain of fibrinogen such that no fibrin polymer is formed in vivo, allowing for the first time the differentiation of the role of fibrinogen vs fibrin oligomer or polymer in antimicrobial host defense and in hemostasis/thrombosis. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2015-08-662551
更新日期:2015-10-22 00:00:00
abstract::Transferrin receptor 2 (TfR2) is a type 2 transmembrane protein expressed in hepatocytes that binds iron-bound transferrin (Tf). Mutations in TfR2 cause one form of hereditary hemochromatosis, a disease in which excessive absorption of dietary iron can lead to liver cirrhosis, diabetes, arthritis, and heart failure. T...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2477
更新日期:2004-12-15 00:00:00
abstract::Fas ligand (FasL) and perforin pathways not only are the major mechanisms of T cell-mediated cytotoxicity but also are involved in homeostatic regulation of these T cells. In the present study, we tested whether CD8+ donor T cells that are deficient in both perforin and FasL (cytotoxic double deficient [cdd]) could in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-08-3036
更新日期:2005-03-01 00:00:00
abstract::We have investigated the role of adenosine diphosphate (ADP) receptors in the adhesion, activation, and aggregation of platelets perfused over immobilized von Willebrand factor (VWF) under high shear stress. Blocking P2Y(1) prevented stable platelet adhesion and aggregation, indicative of a complete inhibition of alph...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-03-1145
更新日期:2004-11-15 00:00:00
abstract::As a dendritic cell (DC) matures, it becomes more potent as an antigen-presenting cell. This functional change is accompanied by a change in DC immunophenotype. The signal transduction events underlying this process are poorly characterized. In this study, we have investigated the signal transduction pathways involved...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Erythropoietin (EPO) is required for the survival and expansion of red blood cell progenitor cells and supports continued differentiation of these committed progenitors to mature red blood cells. After binding to its cognate receptor, EPO promotes receptor homodimerization, activation of receptor-associated JAK2, subs...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-10-15 00:00:00
abstract::In the present study, Epstein-Barr virus (EBV) isolates from 18 malignant tumors (angioimmunoblastic lymphadenopathy [AILD], n = 4; Hodgkin's disease [HD], n = 3; pleomorphic T-cell non-Hodgkin's lymphoma [T-NHL], n = 1; B-cell non-Hodgkin's lymphoma [B-NHL], n = 8; gastric carcinoma, n = 2) as well as from 10 tonsils...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-02-15 00:00:00
abstract::Human CD100, the first semaphorin identified in the immune system, is a transmembrane protein involved in T-cell activation. In the present study, we showed that activation of peripheral blood or tonsillar B lymphocytes induced the expression of CD100 in CD38(+)CD138(-) cell populations, including in CD148(+) subpopul...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00