Abstract:
BACKGROUND:Frequencies of alloreactive T cells determined by limiting dilution assays (LDA) may not adequately reflect the donor-reactive immune status in transplant recipients. To reevaluate LDA frequencies, we developed a flow cytometry test for direct determination of alloreactive T-cell frequencies and compared these frequencies with classical LDA estimates of frequencies. METHODS:For determination of frequencies by flow cytometry, peripheral blood lymphocytes (or lymphocytes taken from primary mixed lymphocyte culture) were stimulated with either Epstein-Barr virus-transformed lymphoblastoid cell lines or T cell-depleted spleen cells and stained for intracellular interferon (IFN)-gamma production and CD69. In lung transplant recipients, frequencies of IFN+ alloreactive T cells were compared with LDA frequencies, that is, cytotoxic T lymphocyte precursors and helper T lymphocyte precursors. RESULTS:With flow cytometry, alloreactive T cells were detected after overnight allostimulation as IFN-gamma CD69bright cells (range, 0.1-0.58% and 0.1-0.66% of total CD4 and CD8 cells, respectively). Frequencies increased 25-fold or more when lymphocytes were prestimulated in primary mixed lymphocyte culture before testing. After lung transplantation, mean donor-specific IFN+ CD8 T-cell frequencies did not decrease as mean donor-specific LDA cytotoxic T lymphocyte precursor frequencies, whereas no difference was seen in pretransplantation samples or third-party-specific frequencies at both time points. Mean frequencies of IFN+ CD4 did not differ from helper T lymphocyte precursors at both time points, but frequencies did not correlate. CONCLUSIONS:The flow cytometry test allows a direct measurement of alloreactive T-cell frequencies and demonstrates a discrepancy between donor-specific IFN+ CD8 T-cell frequencies and LDA CLTp after transplantation. This may be a result of the existence of "functional diverse" alloreactive T cells or of activation-induced cell death of donor-reactive T cells during long (LDA) culturing, which is avoided in the flow cytometry test.
journal_name
Transplantationjournal_title
Transplantationauthors
De Haan A,Van Der Gun I,Van Der Bij W,De Leij LF,Prop Jdoi
10.1097/00007890-200208270-00023subject
Has Abstractpub_date
2002-08-27 00:00:00pages
562-70issue
4eissn
0041-1337issn
1534-6080journal_volume
74pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Humanized Fc receptor (FcR)-nonbinding antibodies against CD3 are promising immunosuppressive agents that may overcome both the neutralizing response to and the cytokine release syndrome seen with conventional monoclonal antibodies against CD3. In addition, evidence from several murine models suggests that t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200104150-00020
更新日期:2001-04-15 00:00:00
abstract::In a prospective longitudinal study, detection of cytomegalovirus (CMV) DNA in plasma (plasma polymerase chain reaction [PCR]) was compared with PCR of CMV DNA in peripheral blood leukocytes (PBL PCR), the CMV pp65 antigenemia assay, and viral cultures from blood, urine, and throat of 29 patients, 14 of whom received ...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199707150-00020
更新日期:1997-07-15 00:00:00
abstract:BACKGROUND:A quantitative model of immunosuppression was previously developed based on the rejection of the allogeneic A/J murine tumor sarcoma 1 (Sa1) in immunocompetent mice. Here, the model is used to evaluate the immunologic mechanisms of graft rejection and to determine the potential of this model to detect synerg...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000157361.50830.22
更新日期:2005-04-27 00:00:00
abstract::Hemorrhagic cystitis is a well known complication of allogeneic bone marrow transplantation (BMT) and is normally attributed to the use of high-dose cyclophosphamide in the preparative regimen. Hemorrhagic cystitis occurring late after BMT is unlikely to be due to the effects of this conditioning, and probably has an ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198701000-00024
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:Deceased donor kidneys with acute kidney injury (AKI) and deceased after circulatory death kidneys are viable sources of organs. The outcomes of renal transplantation from deceased after circulatory death (DCD) donors with AKI are not known. METHODS:A retrospective review of deceased donor renal transplants...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003317
更新日期:2020-05-15 00:00:00
abstract:BACKGROUND:Chronic allograft nephropathy (CAN) has become the predominant limiting factor for long-term transplant survival. A cardinal histomorphologic correlate for CAN is interstitial fibrosis. Currently, no method has been established in routine use that reliably quantifies the extent of interstitial fibrosis in re...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000078899.62040.E5
更新日期:2003-09-27 00:00:00
abstract::The potential usefulness of verapamil, a calcium channel blocker to renal allograft preservation, was investigated using the isolated perfused rat kidney. Two models of ischemic injury were used. In the first model, rat kidneys were exposed to 40 min of 37 degrees C ischemia on the perfusion circuit. Addition of verap...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198512000-00004
更新日期:1985-12-01 00:00:00
abstract:BACKGROUND:We hypothesized that tissue-engineered contractile cardiomyocyte sheets without a scaffold would show histological and electrical integration with impaired myocardium, leading to the regeneration of infarcted myocardium. METHODS:Neonatal rat cardiomyocytes were cultured on Poly(N-isopropylacrylamide)-grafte...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000181163.69108.dd
更新日期:2005-12-15 00:00:00
abstract::Genetic manipulation of pancreatic islets before transplantation has the potential to alter cellular immunity as well as islet function. The purpose of this study was to examine the feasibility of gene transfer to islets, using replication-defective adenoviral vectors. Newborn mouse islets were infected with AdHCMVsp1...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-01-27 00:00:00
abstract::Acute rejection is a frequent cause of early graft dysfunction in renal transplantation, and serum creatinine is the most important measure of clinical response. However, there is little information on serum creatinine during rejection episodes. In this study, the determinants that might influence the clinical respons...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199507150-00006
更新日期:1995-07-15 00:00:00
abstract::The effect of cyclosporine on hepatic ischemia was investigated. Hepatic ischemia was produced for 90 min in mongrel dogs. Experimental dogs were divided into three groups as follows: group A (control group), group B (CsA pretreatment group), group C (CsA posttreatment group). CsA was administered at a dose of 10 mg/k...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199107000-00024
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:The prevalence of renal posttransplantation amputation and its impact on allograft and patient survival have not been widely reported. METHODS:We used an incident cohort of patients who underwent renal transplantation between June 2004 and September 2009. Amputation data were obtained using Medicare institu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31828e72b4
更新日期:2013-05-27 00:00:00
abstract:BACKGROUND:Recurrent hepatitis C virus (HCV) after liver transplantation (OLT) is a major cause of graft loss in HCV-positive patients. In this study, we evaluated the efficacy and safety of pegylated interferon alfa-2b (peginterferon) and ribavirin treatment for recurrent HCV after OLT and analyzed the influence of an...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/01.tp.0000225827.18034.be
更新日期:2006-07-15 00:00:00
abstract::For maximum use of left liver plus caudate lobe grafts, the short hepatic vein draining the caudate lobe should be reconstructed. We devised a new venoplasty technique. On the graft side, a wide venous orifice with a long cuff was formed by gathering the left, middle, and short hepatic veins using a conduit vein graft...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/01.tp.0000244729.39485.7c
更新日期:2007-01-27 00:00:00
abstract::Patients undergoing primary cadaveric kidney transplantation were followed for 1 year as part of a phase II, multicenter, open-label concentration-ranging trial of FK506 and cyclosporine. One hundred twenty patients were randomly assigned to a cyclosporine-based regimen or one of three FK506-based regimens designed to...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/00007890-199606150-00005
更新日期:1996-06-15 00:00:00
abstract:BACKGROUND:Administration of donor bone marrow (BM)-derived dendritic cell (DC) progenitors (DCp) that are major histocompatibility complex (MHC) class II+ but costimulatory molecule (CD40, CD80, CD86)-deficient can prolong mouse heart allograft survival This is associated with microchimerism and inhibition of antidono...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199812270-00040
更新日期:1998-12-27 00:00:00
abstract::Discordant xenogeneic organ transplantation is a potential solution to the critical shortage of suitable donor organs. However, clinical application of xenotransplantation with physiologically suitable organs such as those from the pig, is currently limited by the lack of agents to prevent antibody and complement-medi...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-05-27 00:00:00
abstract::Heart transplantation is considered nowadays the gold standard in the therapy of chronic and terminal heart insufficiency. Primary organ failure after heart transplantation is a severe complication generally related to prolonged ischemia time, poor quality of the organ, or acute rejection. All these factors can potent...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000233845.15508.89
更新日期:2006-10-27 00:00:00
abstract:BACKGROUND:To better understand how different histologic patterns of allograft inflammation found on biopsies of human cardiac allografts progress to high-grade rejection, we undertook a statistical analysis of our institutional database to detect statistical patterns among different types of myocardial allograft infla...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200206270-00014
更新日期:2002-06-27 00:00:00
abstract::A patient with end-stage renal disease and known benign monoclonal gammopathy underwent kidney transplantation at Westchester County Medical Center, Valhalla, NY. After surgery, during routine follow-up, the patient had laboratory evidence of frank multiple myeloma. However, she did not show any clinical signs or symp...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/00007890-199612150-00008
更新日期:1996-12-15 00:00:00
abstract:BACKGROUND:In the present study we examined the effect of the timing of marrow infusion on engraftment in nonmyeloablatively conditioned mice. METHODS:B10 mice were conditioned with decreasing doses of total body irradiation (TBI) and reconstituted with bone marrow cells (BMCs) from major histocompatibility complex-di...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000116414.66171.81
更新日期:2004-03-27 00:00:00
abstract::Sera obtained before transplantation from 52 consecutive renal allograft recipients were tested for antibody-dependent cell-mediated cytotoxicity (ADCC) and for complement-dependent cytotoxic antibodies (CDC). A D locus antigen-defined lymphoblastoid cell panel (B lymphoblastoid cell panel) was used as targets for the...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract:BACKGROUND:Knowledge on the reinnervation of transplanted organs is scarce, and the aim of the study was therefore to evaluate to what degree syngeneic pancreas grafts were reinnervated in rats. METHODS:Syngeneic pancreatico-duodenal transplantations were performed in normoglycemic Wistar-Furth rats. Native and transp...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200101150-00002
更新日期:2001-01-15 00:00:00
abstract::One major limitation in pancreatic islet transplantation is availability of donor tissue. Donor shortage is exacerbated by islet apoptosis from the stresses of islet isolation and transplantation. Furthermore, the side effects of immunosuppressive drugs preclude transplants into patients whose diabetes is controlled b...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000132283.95107.9c
更新日期:2004-05-27 00:00:00
abstract:BACKGROUND:The use of anti-B-cell and T-cell immunosuppressive agents leads to only a few weeks' survival of mouse-to-rat cardiac xenografts. METHODS:BALB/c cardiac xenografts were transplanted to Lewis rats treated with cyclosporine (CsA) and/or cobra venom factor (CVF). RESULTS:CsA alone did not prolong xenograft s...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199805150-00012
更新日期:1998-05-15 00:00:00
abstract::This study evaluated the utility of duplex Doppler sonograms (DS) and the resistive index (RI) in the identification and differential diagnosis of various causes of renal allograft dysfunction. The efficacy of DS and RI was studied either during acute episodes of allograft dysfunction or during periodic posttransplant...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199012000-00015
更新日期:1990-12-01 00:00:00
abstract::The survival of semiallogeneic or fully allogeneic rat kidney allografts was prolonged in some strain combinations with immune complexes formed with hapten-conjugated alloantigens (TNP-Ag) of donor origin and a mouse monoclonal antihapten antibody (anti-TNP). Complexes were administered i.v. at the time of operation--...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198311000-00001
更新日期:1983-11-01 00:00:00
abstract:BACKGROUND:We have previously shown successful engraftment of allogeneic hematopoietic stem cells (HSCs) when transplanted across the major histocompatibility antigen barriers if transplanted along with a preparation of facilitator cells (osteoblasts). We have investigated whether or not fully allogeneic HSCs from heal...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200009270-00004
更新日期:2000-09-27 00:00:00
abstract:BACKGROUND:Kidney donation after cardiac death leads to vascular damage as a result of warm ischemia, affecting renovascular circulating volume. Novel ultrasound dilution techniques may be used to measure renovascular circulating volumes during hypothermic machine perfusion of donor kidneys. METHODS:Renovascular circu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31828a46f7
更新日期:2013-05-15 00:00:00
abstract:BACKGROUND:Sotrastaurin (STN), a novel oral protein kinase C inhibitor that inhibits early T-cell activation, was assessed in non-human primate recipients of life-supporting kidney allografts. METHODS:Cynomolgus monkey recipients of life-supporting kidney allografts were treated orally with STN alone or in combination...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31823cf92f
更新日期:2012-01-27 00:00:00