Simultaneous determination of free testosterone and testosterone bound to non-sex-hormone-binding globulin by equilibrium dialysis.

Abstract:

:We describe a procedure based on equilibrium dialysis that allows the simultaneous determination of free testosterone and testosterone bound to non-sex-hormone-binding globulin (non-SHBG) in plasma. After saturating SHBG with 5 alpha-dihydrotestosterone (DHT) according to a technique recently described, the percentage of free testosterone in the treated and the untreated samples is measured by equilibrium dialysis with use of a semiautomated instrument that allows rigorous standardization of the experimental conditions. The present method is simpler and faster than the previously described technique in which, after the saturation of SHBG with DHT, the unbound fractions were measured by centrifugal ultrafiltration dialysis. The method is also reproducible and suited for the analysis of a large number of samples. The technique has been applied to the determination of the fractional distribution of testosterone in plasma pools from normally menstruating, pregnant, and postmenopausal women and from normal men.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Barini A,Liberale I,Menini E

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

938-41

issue

6

eissn

0009-9147

issn

1530-8561

journal_volume

39

pub_type

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