CD40 ligation and phagocytosis differently affect the differentiation of monocytes into dendritic cells.


:That monocytes can differentiate into macrophages or dendritic cells (DCs) makes them an essential link between innate and adaptive immunity. However, little is known about how interactions with pathogens or T cells influence monocyte engagement toward DCs. We approached this point in cultures where granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4 induced monocytes to differentiate into immature DCs. Activating monocytes with soluble CD40 ligand (CD40L) led to accelerated differentiation toward mature CD83(+) DCs with up-regulated human leukocyte antigen-DR, costimulatory molecules and CD116 (GM-CSF receptor), and down-regulation of molecules involved in antigen capture. Monocytes primed by phagocytosis of antibody-opsonized, killed Escherichia coli differentiated into DCs with an immature phenotype, whereas Zymosan priming yielded active DCs with an intermediate phenotype. Accordingly, DCs obtained from cultures with CD40L or after Zymosan priming had a decreased capacity to endocytose dextran, but only DCs cultured with CD40L had increased capacity to stimulate allogeneic T cells. DCs obtained after E. coli or Zymosan priming of monocytes produced high levels of proinflammatory tumor necrosis factor alpha and IL-6 as well as of regulatory IL-10, but they produced IL-12p70 only after secondary CD40 ligation. Thus, CD40 ligation on monocytes accelerates the maturation of DCs in the presence of GM-CSF/IL-4, whereas phagocytosis of different microorganisms does not alter and even facilitates their potential to differentiate into immature or active DCs, the maturation of which can be completed upon CD40 ligation. In vivo, such differences may correspond to DCs with different trafficking and T helper cell-stimulating capacities that could differently affect induction of adaptive immune responses to infections.


J Leukoc Biol


Rosenzwajg M,Jourquin F,Tailleux L,Gluckman JC


Has Abstract


2002-12-01 00:00:00












  • In vitro model for hematopoietic progenitor cell homing reveals endothelial heparan sulfate proteoglycans as direct adhesive ligands.

    abstract::Proteoglycans (PGs) play a dominant role within the bone marrow (BM), but their role in homing of transplanted hematopoietic progenitor cells (HPC) is unknown. In this study, the role of heparan sulfate (HS) PGs on BM endothelium as adhesive structures was investigated. HPC (primary CD34+ cells and cell line KG-1a) we...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Netelenbos T,van den Born J,Kessler FL,Zweegman S,Huijgens PC,Drager AM

    更新日期:2003-12-01 00:00:00

  • IFN-beta improves BCG immunogenicity by acting on DC maturation.

    abstract::Given the variable protective efficacy provided by Mycobacterium bovis bacillus Calmette-Guérin (BCG), there is an urgent need to develop new vaccines against tuberculosis. As dendritic cells (DC) play a critical role in initiating and regulating a protective T cell response against the pathogens, the comprehension of...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Giacomini E,Remoli ME,Gafa V,Pardini M,Fattorini L,Coccia EM

    更新日期:2009-03-01 00:00:00

  • Transcription factors that regulate monocyte/macrophage differentiation.

    abstract::Although all the cells in an organism contain the same genetic information, differences in the cell phenotype arise from the expression of lineage-specific genes. During myelopoiesis, external differentiating signals regulate the expression of a set of transcription factors. The combined action of these transcription ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Valledor AF,Borràs FE,Cullell-Young M,Celada A

    更新日期:1998-04-01 00:00:00

  • Regulation of cytosolic phospholipase A2alpha by hsp90 and a p54 kinase in okadaic acid-stimulated macrophages.

    abstract::In resident mouse peritoneal macrophages, group IVA cytosolic phospholipase A(2) (cPLA(2)alpha) mediates arachidonic acid (AA) release and eicosanoid production in response to diverse agonists such as A23187, phorbol myristate acetate, zymosan, and the enterotoxin, okadaic acid (OA). cPLA(2)alpha is regulated by phosp...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Tucker DE,Gijón MA,Spencer DM,Qiu ZH,Gelb MH,Leslie CC

    更新日期:2008-09-01 00:00:00

  • Interleukin-1 beta (IL-1 beta), IL-1 receptor antagonist, and TNF alpha production in whole blood.

    abstract::The ability of an individual to mount defense responses to infection depend in part on the capacity to produce cytokines such as interleukin 1 (IL-1) and tumor necrosis factor (TNF). The specialized equipment, labor intensity, and sterile practice required for the standard in vitro evaluation of cytokine production ca...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Nerad JL,Griffiths JK,Van der Meer JW,Endres S,Poutsiaka DD,Keusch GT,Bennish M,Salam MA,Dinarello CA,Cannon JG

    更新日期:1992-12-01 00:00:00

  • The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease.

    abstract::The biology of cells of the mononuclear phagocyte system has been studied extensively in the mouse. Studies of the pig as an experimental model have commonly been consigned to specialist animal science journals. In this review, we consider some of the many ways in which the innate immune systems of humans differ from ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Fairbairn L,Kapetanovic R,Sester DP,Hume DA

    更新日期:2011-06-01 00:00:00

  • Expression and visualization during cell cycle progression of alkaline phosphatase in B lymphocytes from C3H/HeJ mice.

    abstract::The expression of alkaline phosphatase (APase) activity by purified B cells from lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice was determined. Optimal APase activity was expressed after costimulation with interleukin-5 and dextran sulfate (DXS), whereas LPS, which is highly effective on B lymphocytes from norma...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Souvannavong V,Brown S,Sarih M,Adam A

    更新日期:1994-05-01 00:00:00

  • Diamide primes neutrophils for enhanced release of superoxide anion: relationship to S-thiolation of cellular proteins.

    abstract::Stimulation of the respiratory burst in phagocytes induces the formation of mixed disulfides between sulfhydryl groups of proteins and low-molecular-weight thiols. We hypothesized that this process (S-thiolation) might be involved in turning off the respiratory burst. However, induction of S-thiolation by pretreatment...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Moriguchi T,Seres T,Ravichandran V,Sasada M,Johnston RB Jr

    更新日期:1996-08-01 00:00:00

  • Macrophage-mediated tumor lysis induced by loach egg lectin.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Yamazaki M,Okutomi T,Sakakibara F,Kawauchi H,Mizuno D

    更新日期:1984-03-01 00:00:00

  • Liver natural killer and natural killer T cells: immunobiology and emerging roles in liver diseases.

    abstract::Hepatic lymphocytes are enriched in NK and NKT cells that play important roles in antiviral and antitumor defenses and in the pathogenesis of chronic liver disease. In this review, we discuss the differential distribution of NK and NKT cells in mouse, rat, and human livers, the ultrastructural similarities and differe...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Gao B,Radaeva S,Park O

    更新日期:2009-09-01 00:00:00

  • Inhibition of apoptosis and prolongation of neutrophil functional longevity by inflammatory mediators.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lee A,Whyte MK,Haslett C

    更新日期:1993-10-01 00:00:00

  • Selective sensitivity of macrophages to cytotoxicity by inhibitors of macromolecular synthesis: induction of apoptosis.

    abstract::Initial studies designed to measure the effect of inhibiting RNA synthesis by dactinomycin on macrophage functions revealed that the cells were uniformly killed at concentrations that have been routinely used to inhibit RNA synthesis in other cell types. We, thus, determined the dose curve for the cytotoxicity of dact...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lewis JG,Adams DO,Fan S

    更新日期:1995-04-01 00:00:00

  • The essential role of the in situ immune reaction in human colorectal cancer.

    abstract::Colorectal cancer is the second most common cause of cancer-related death. Novel prognostic factors should be identified and validated to refine the present tumor-node-metastasis system. The presence of immune cells infiltrating colorectal cancers is a common phenomenon. However, the current belief is that clinically ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Pagès F,Galon J,Fridman WH

    更新日期:2008-10-01 00:00:00

  • Pivotal Advance: eosinophil infiltration of solid tumors is an early and persistent inflammatory host response.

    abstract::Tumor-associated eosinophilia has been observed in numerous human cancers and several tumor models in animals; however, the details surrounding this eosinophilia remain largely undefined and anecdotal. We used a B16-F10 melanoma cell injection model to demonstrate that eosinophil infiltration of tumors occurred from t...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Cormier SA,Taranova AG,Bedient C,Nguyen T,Protheroe C,Pero R,Dimina D,Ochkur SI,O'Neill K,Colbert D,Lombari TR,Constant S,McGarry MP,Lee JJ,Lee NA

    更新日期:2006-06-01 00:00:00

  • MC148 encoded by human molluscum contagiosum poxvirus is an antagonist for human but not murine CCR8.

    abstract::The viral CC chemokines MC148, encoded by the poxvirus molluscum contagiosum, and viral macrophage inflammatory protein (vMIP)-I and vMIP-II, encoded by human herpesvirus 8, were probed on the murine CC receptor (CCR) 8 in parallel with human CCR8. In calcium mobilization assays, vMIP-I acted as a high-affinity agonis...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lüttichau HR,Gerstoft J,Schwartz TW

    更新日期:2001-08-01 00:00:00

  • Targeted STAT3 disruption in myeloid cells alters immunosuppressor cell abundance in a murine model of spontaneous medulloblastoma.

    abstract::Although the immune system may provide early protection against cancer, tumors may exploit the healing arm of the immune system to enhance their growth and metastasis. For example, myeloid derived suppressor cells (MDSCs) are thought to promote tumor growth by several mechanisms, including the suppression of T cell ac...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Abad C,Nobuta H,Li J,Kasai A,Yong WH,Waschek JA

    更新日期:2014-02-01 00:00:00

  • Contrasting effects of alpha- and beta-androstenediol on oncogenic myeloid cell lines in vitro.

    abstract::The in vitro effects of 17 alpha AED, the isomer of 5-androstene-3 beta,17 beta diol (17 beta AED) on the basal growth of murine RAW 264.7, P388D1, and human HL-60 cells were investigated. 17 alpha AED treatment of RAW cells for 48 h reduced total cell number without increasing cell death as detected by trypan blue ex...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Huynh PN,Loria RM

    更新日期:1997-08-01 00:00:00

  • Pivotal advance: IgE accelerates in vitro development of mast cells and modifies their phenotype.

    abstract::Antigen-dependent activation of IgE-bound mast cells is critical for immediate hypersensitivity and other allergic disorders. Recent studies have revealed the effects of monomeric IgEs on mast cell survival and activation. Furthermore, IgE molecules exhibit a wide range of heterogeneity in the ability to induce mast c...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kashiwakura J,Xiao W,Kitaura J,Kawakami Y,Maeda-Yamamoto M,Pfeiffer JR,Wilson BS,Blank U,Kawakami T

    更新日期:2008-08-01 00:00:00

  • CD11b/CD18-coated microspheres attach to E-selectin under flow.

    abstract::Neutrophils can attach to E-selectin under flow. Proposed ligands for E-selectin carry SLe(x)-type glycans. The leukocyte beta2 integrins are glycosylated with SLe(x). Thus, we speculated that beta2 integrins could support attachment to E-selectin. To test this hypothesis, we coated 10-microm-diameter microspheres wit...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Crutchfield KL,Shinde Patil VR,Campbell CJ,Parkos CA,Allport JR,Goetz DJ

    更新日期:2000-02-01 00:00:00

  • Infiltrating neutrophils induce allospecific CTL in response to immunization with apoptotic cells via MCP-1 production.

    abstract::Our previous studies demonstrated that i.p. injection of late apoptotic P388 cells caused phagocytosis by macrophages and transient infiltration of neutrophils into the peritoneal cavity. As neutrophils are known to function as effectors as well as regulators in the immune response, we examined the roles of infiltrati...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Shiratsuchi Y,Iyoda T,Tanimoto N,Kegai D,Nagata K,Kobayashi Y

    更新日期:2007-02-01 00:00:00

  • Shared features of transcription: mutational analysis of the eosinophil/basophil Charcot-Leyden crystal protein gene promoter.

    abstract::The lineage-specific Charcot-Leyden crystal (CLC) protein is found in human eosinophils and basophils where it comprises 7-10% of the cellular protein content. Previous work from our laboratory has identified the motif GGAGA[A/G] as a powerful enhancer of gene transcription ill two eosinophil ribonuclease genes. To ev...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Dyer KD,Rosenberg HF

    更新日期:2000-05-01 00:00:00

  • Colony-stimulating factors increase resistance to atypical mycobacteria in resistant mice, whereas they decrease resistance in susceptible strains of mice.

    abstract::Inbred strains of mice, notably the susceptible C57BL/6 and the resistant A/J strains of mice, were infected with a strain of Mycobacterium evium. The infection in the visceral organs of mice was then studied, and the effect of colony-stimulating factors, i.e., interleukin-3 (IL-3), granulocyte-macrophage colony-stimu...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Denis M

    更新日期:1991-09-01 00:00:00

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    abstract::Enhancer of zeste homolog 2 (EZH2) is aberrantly expressed or mutated in multiple types of cancer cells and plays an oncogenic role in tumorigenesis and development in most cancers. Results from pilot clinical studies have implied that EZH2 inhibitors have therapeutic potential against some cancers. However, the exact...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Shao FF,Chen BJ,Wu GQ

    更新日期:2020-10-11 00:00:00

  • Low Tregs: A targetable risk factor for life-threatening cardiovascular complications after major noncardiac surgery.

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    journal_title:Journal of leukocyte biology

    pub_type: 评论,社论


    authors: Kyaw T,Bobik A

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  • Donor- but not host-derived interleukin-10 contributes to the regulation of experimental graft-versus-host disease.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Tawara I,Sun Y,Liu C,Toubai T,Nieves E,Evers R,Alrubaie M,Mathewson N,Tamaki H,Reddy P

    更新日期:2012-04-01 00:00:00

  • Regulation of TNF mediated antiapoptotic signaling in human neutrophils: role of delta-PKC and ERK1/2.

    abstract::TNF is implicated in the suppression of neutrophil apoptosis during sepsis. Multiple signaling pathways are involved in TNF-mediated antiapoptotic signaling; a role for the MAP kinases (MAPK), ERK1/2, and p38 MAPK has been suggested. Antiapoptotic signaling is mediated principally through TNF receptor-1 (TNFR-1), and ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kilpatrick LE,Sun S,Mackie D,Baik F,Li H,Korchak HM

    更新日期:2006-12-01 00:00:00

  • Infection of a murine T-cell line with a retrovirus carrying c-myc decreases the interleukin-2 cell dependence by a nonautocrine mechanism.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Shapiro IM,Winberg G,Stefanescu I,Wigzell H,Menezes J

    更新日期:1988-12-01 00:00:00

  • PKC-ε pseudosubstrate and catalytic activity are necessary for membrane delivery during IgG-mediated phagocytosis.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Wood TR,Chow RY,Hanes CM,Zhang X,Kashiwagi K,Shirai Y,Trebak M,Loegering DJ,Saito N,Lennartz MR

    更新日期:2013-07-01 00:00:00

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Hadjokas N,Bayer C,Nielson CP

    更新日期:1992-08-01 00:00:00

  • Assessment of antibody-dependent respiratory burst activity from mouse neutrophils on Plasmodium yoelii malaria challenge outcome.

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    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Llewellyn D,de Cassan SC,Williams AR,Douglas AD,Forbes EK,Adame-Gallegos JR,Shi J,Pleass RJ,Draper SJ

    更新日期:2014-02-01 00:00:00