Abstract：
:Because free factor VIIa is inactivated only very slowly by a plasma concentration of antithrombin III (AT III) even in the presence of heparin, it has been assumed that AT III plays no significant role in regulating the initiation of tissue factor-dependent blood coagulation. However, in the present study, we present evidence that factor VIIa bound to tissue factor, unlike free factor VIIa, is readily inactivated by AT III in the presence of heparin. In a reaction mixture containing calcium ions and approximately equimolar concentrations of relipidated tissue factor (8.9 nmol/L) and factor VIIa (10 nmol/L), AT III (100 micrograms/mL) plus heparin (1 U/mL) inhibited 50% of the factor VIIa coagulant activity of the reaction mixture within 5 minutes. AT III/heparin was also shown to inhibit the catalytic activity towards factor X of factor VIIa/tissue factor complexes formed on monolayers of an ovarian carcinoma cell line (OC-2008) that constitutively expresses surface membrane tissue factor. AT III, even in the absence of exogenously added heparin, substantially inhibited the functional activity of factor VIIa/cell surface tissue factor complexes on intact monolayers. AT III alone and AT III/heparin, to a greater extent, also inhibited factor VIIa on "nonfunctional" factor VIIa/tissue factor complexes on intact monolayers, with resultant inhibition of their expression of factor VIIa/tissue factor catalytic activity toward factor X after cell lysis. The potential physiologic significance of these findings is discussed.
journal_name
Bloodjournal_title
Bloodauthors
Rao LV,Rapaport SI,Hoang ADsubject
Has Abstractpub_date
1993-05-15 00:00:00pages
2600-7issue
10eissn
0006-4971issn
1528-0020journal_volume
81pub_type
杂志文章相关文献
BLOOD文献大全abstract：:Several lines of evidence indicate that macrophage inflammatory protein-1alpha (MIP-1alpha) modulates the proliferation of hematopoietic progenitor cells, depending on their maturational stages. To clarify the mechanisms for the modulation of hematopoiesis by this chemokine, we examined the expression of a receptor fo...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-07-15 00:00:00
abstract：:Myeloma bone disease is due to interactions of myeloma cells with the bone marrow microenvironment, and is associated with pathologic fractures, neurologic symptoms and hypercalcemia. Adjacent to myeloma cells, the formation and activation of osteoclasts is increased, which results in enhanced bone resorption. The rec...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2002-09-2684
更新日期：2003-03-15 00:00:00
abstract：:We have investigated the role of plasminogen activator inhibitor 1 (PAI-1) in the regulation of fibrinolysis using a model thrombus composed of thrombin-stimulated platelets, fibrin(ogen), plasminogen, and recombinant tissue-type plasminogen activator. Laser light scattering kinetic measurements showed that clot lysis...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:The evidence for a regulatory role of acidic isoferritins on hemopoiesis is not entirely consistent with our knowledge of ferritin biochemistry, and no clear picture of this phenomeonon has emerged. In the present study, we have been unable to confirm a consistent effect of purified heart (acidic), spleen (basic), or ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:Angiogenesis, the formation of new capillaries from preexisting blood vessels, is a multistep, highly orchestrated process involving vessel sprouting, endothelial cell migration, proliferation, tube differentiation, and survival. Eicosanoids, arachidonic acid (AA)-derived metabolites, have potent biologic activities o...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:The Wiskott-Aldrich syndrome (WAS) is a severe immunodeficiency and platelet deficiency disease arising from mutation(s) in the WASP gene, which in normal cells encodes an intracellular protein able to interact with other proteins relevant to the control of cytoskeleton organization. Immunodeficiency is mainly due to ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-10-15 00:00:00
abstract：:Ferroportin (FPN) mediates iron export from cells; FPN mutations are associated with the iron overloading disorder hemochromatosis. Previously, we found that the A77D, V162del, and G490D mutations inhibited FPN activity, but that other disease-associated FPN variants retained full iron export capability. The peptide h...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-02-0561
更新日期：2005-08-01 00:00:00
abstract：:The genetic diversity of the human immunodeficiency virus (HIV) isolated from transfusion-associated AIDS patients has been examined. Restriction enzyme mapping studies of integrated proviral DNA of donor and recipient origin demonstrated genomic variation between isolates. Analysis of the molecularly cloned viral gen...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Conditioned medium (CM) obtained from a human hepatoma cell line, SK-HEP-1, contains colony-stimulating factors (CSFs) active on murine and human bone marrow-derived granulocyte and macrophage colony-forming units (CFU-GM) and a factor capable of inducing granulocyte-macrophage differentiation (GM-DF) of murine myelom...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:People with hemophilia were formerly at very high risk of infection with hepatitis C virus (HCV). Approximately 20% of HCV-infected patients spontaneously clear the virus. To identify correlates of spontaneous clearance of HCV, we studied a cohort of HCV-infected hemophilic subjects without human immunodeficiency viru...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：10.1182/blood-2005-07-2781
更新日期：2006-02-01 00:00:00
abstract：:The anemia of chronic disease (ACD) results from 3 major processes: slightly shortened red cell survival, impaired reticuloendothelial system iron mobilization, and impaired erythropoiesis. Hepcidin is an acute-phase protein with specific iron regulatory properties, which, along with the anemia seen with increased hep...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-07-2854
更新日期：2006-04-01 00:00:00
abstract：:Interleukin-3 (IL-3) has been shown to be a promising agent in the stimulation of bone marrow regeneration following myeloablative therapy. The biologic half-life of this agent is very short (5 to 15 minutes), which limits the effectiveness of low-dose therapy. Here we show that the biologic effects of low-dose IL-3 i...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-08-15 00:00:00
abstract：:Both extrinsic and intrinsic mechanisms tightly govern hematopoietic stem cell (HSC) decisions of self-renewal and differentiation. However, transcription factors that can selectively regulate HSC self-renewal division after stress remain to be identified. Slug is an evolutionarily conserved zinc-finger transcription ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2009-07-232934
更新日期：2010-03-04 00:00:00
abstract：:Mutations in the Bcr-Abl kinase domain may cause, or contribute to, resistance to tyrosine kinase inhibitors (TKIs) in chronic myeloid leukemia patients. Recommendations aimed to rationalize the use of BCR-ABL mutation testing in chronic myeloid leukemia have been compiled by a panel of experts appointed by the Europe...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2010-12-326405
更新日期：2011-08-04 00:00:00
abstract：:Leukocyte alpha4beta1 integrins regulate hematopoietic and lymphoid development, as well as the emigration of circulating cells to sites of inflammation. Because vascular cell adhesion molecule-1 (VCAM-1) binding to high-affinity alpha4beta1 is stable, these integrins can be detected and selectively precipitated from ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-12-4402
更新日期：2004-11-01 00:00:00
abstract：:Advances in immune assessment, including the development of T-cell receptor excision circle (TREC) assays of thymopoiesis, cytokine-flow cytometry assays of T-cell function, and higher-order phenotyping of T-cell maturation subsets have improved our understanding of T-cell homeostasis. Limited data exist using these m...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2007-05-092130
更新日期：2007-12-15 00:00:00
abstract：:Alloreactive T cells present in a bone marrow transplant are responsible for graft-versus-host disease (GVHD), but their depletion is associated with impaired engraftment, immunosuppression, and loss of the graft-versus-leukemia effect. We developed a therapeutic strategy against GVHD based on the selective destructio...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-06-15 00:00:00
abstract：:In this issue of Blood, Leonhardt et al report that neovascularization during graft-versus-host disease (GVHD) is regulated by av integrins and the micro RNA miR-100. ...
journal_title：Blood
pub_type： 评论,杂志文章
doi：10.1182/blood-2013-01-480483
更新日期：2013-04-25 00:00:00
abstract：:Hairy cell leukemia variant (HCLv) presents with high disease burden, lack of typical antigens like CD25, and poor response to standard treatments like cladribine. Occasionally, patients with classic HCL respond poorly. Clinical and molecular features of HCL and HCLv has not been compared. Rearrangements expressing im...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2009-01-201731
更新日期：2009-11-19 00:00:00
abstract：:Sixty-five patients with hairy cell leukemia underwent splenectomy; 27 had a complete remission as defined by a return in WBC, RBC, and platelet counts to a defined level, and 38 had a partial remission with a return of only one or two of these parameters to the defined level. The 5-yr actuarial survival for all patie...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
abstract：:The cellular immune defect in untreated Hodgkin's disease (HD) has long been recognized. This defect appears to be responsible for at least some of the morbidity and ultimately the mortality associated with the disease. In recent years, many studies have shown that the T cell component of the immune response is the ap...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:Caveolin-1 (Cav-1) is a key organizer of membrane specializations and a scaffold protein that regulates signaling in multiple cell types. We found increased Cav-1 expression in human and murine T cells after allogeneic hematopoietic cell transplantation. Indeed, Cav-1(-/-)donor T cells caused less severe acute graft-v...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2015-09-672428
更新日期：2016-04-14 00:00:00
abstract：:Lymphatic endothelial cells (LECs) induce peripheral tolerance by direct presentation to CD8 T cells (T(CD8)). We demonstrate that LECs mediate deletion only via programmed cell death-1 (PD-1) ligand 1, despite expressing ligands for the CD160, B- and T-lymphocyte attenuator, and lymphocyte activation gene-3 inhibitor...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-04-427013
更新日期：2012-12-06 00:00:00
abstract：:It has now been 3 years since the von Willebrand factor (VWF)-cleaving protease implicated in thrombocytopenic purpura (TTP) pathogenesis was identified as ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type 1 motif 13). More than 50 ADAMTS13 mutations resulting in familial TTP have been reported...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2004-10-4097
更新日期：2005-07-01 00:00:00
abstract：:Fifty patients with Philadelphia chromosome-positive (Ph(+)) chronic myelogenous leukemia (CML) in early chronic phase received imatinib mesylate, 400 mg orally daily. After a median follow-up of 9 months, 49 patients (98%) achieved a complete hematologic response and 45 patients (90%) achieved a major cytogenetic res...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2002-02-0545
更新日期：2003-01-01 00:00:00
abstract：:A global phase 3 study evaluated the pharmacokinetics, efficacy, and safety of recombinant fusion protein linking coagulation factor IX with albumin (rIX-FP) in 63 previously treated male patients (12-61 years) with severe hemophilia B (factor IX [FIX] activity ≤2%). The study included 2 groups: group 1 patients recei...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：10.1182/blood-2015-09-669234
更新日期：2016-04-07 00:00:00
abstract：:Intravenous immunoglobulin (IVIgG) causes an acute rise in the platelet count in the majority of patients with chronic immune thrombocytopenic purpura (ITP) but the mechanism(s) of action is still unknown. We evaluated the ability of three different IVIgG preparations to inhibit the in vitro binding of autoantibody to...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-11-15 00:00:00
abstract：:Depressed cellular immune function and increased susceptibility to infection characterize familial erythrophagocytic lymphohistiocytosis (FEL), a usually fatal autosomal recessive disease. One component of the immunodeficiency is plasma-mediated inhibition of lymphocyte proliferation. We have tested whether repeated p...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:Platelet von Willebrand factor (vWF) has been suggested to play an important role in the hemostatic process. Clinical and experimental data indicate that bleeding time (BT) and platelet-vessel wall interaction cannot be normalized unless the defect of platelet vWF is also corrected. We have examined the effect of norm...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:The formation and functioning of the factor X activating complex on the surface of cultured human venous endothelial cells (HVEC) were investigated. To the HVEC monolayer human factors IXa, VIII, X, CaCl2, and S-2222 were added, and a gradually increasing activation of factor X was observed. The maximum activity of 88...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
