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Contractile responses of the human umbilical artery to KCl and serotonin in Ca-free medium and the effects of levcromakalim.

Abstract：

:It is known that K(ATP) channel openers inhibit the release and refilling of Ca(2+) from intracellular stores. The present study was designed to test the effects of levcromakalim in human umbilical artery (HUA) rings stimulated by serotonin (5-HT) and KCl in Ca-free medium. Umbilical cords were obtained at vaginal or cesarean deliveries from healthy, term pregnancies. After the isolation, HUA rings were placed in organ baths in solution with indomethacin (10(-5) M) and N(G)-nitro-L-arginine methyl ester (L-NAME) (10(-3) M) at 37 degrees C and aerated with 95% O(2) and 5% CO(2) for the measurement of isometric force. In Ca-free solution with Ethylene glycol-bis (ss-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) (2 mM) the contractions produced by 5-HT (10(-6) M) and KCl (40 mM) decreased significantly. Afterwards, HUA rings were treated with 5-HT and KCl in repeated manner in Ca-free medium. In contrast to KCl, 5-HT induced contractions reduced in each application, progressively. Levcromakalim (10(-4) M) abolished the contractions elicited by 5-HT. On the other hand, levcromakalim had a little but significant inhibitory effect on KCl induced contraction in Ca-free medium. These results suggest that Ca(2+) is not the only transduction pathway in KCl produced contractions of HUA smooth muscle cells.

journal_name

Life Sci

journal_title

Life sciences

authors

Tufan H,Ayan-Polat B,Tecder-Unal M,Polat G,Kayhan Z,Oğüş E

doi

10.1016/s0024-3205(02)02382-2

subject

Has Abstract

pub_date

2003-02-07 00:00:00

pages

1321-9

issue

12

eissn

0024-3205

issn

1879-0631

pii

S0024320502023822

journal_volume

72

pub_type

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