Abstract:
:In isolated hepatic nuclei, the heavy metals Zn2+ and Cu2+ (10 microM) inhibited Ca2+ uptake and caused a prompt release of Ca2+ from preloaded nuclei in a concentration-dependent manner, with Zn2+ being more effective than Cu2+. The sulfhydryl group reducing agent dithiothreitol (DTT) protected the nuclei from the effects of heavy metals; DDT (1 mM) almost completely blocked Zn(2+)- or Cu(2+)-induced Ca2+ release and inhibition of Ca2+ uptake. The sulfhydryl modifying reagent N-ethylmaleimide (NEM; 0.2 mM) also caused Ca2+ release, but it did not have an appreciable effect on Ca2+ uptake. Furthermore, in the presence of NEM heavy metals did not evoke Ca2+ release. The present study demonstrates that Zn2+ and Cu2+ have a stimulatory effect on Ca2+ release from isolated rat liver nuclei, and that the SH group may play an important role in the Ca(2+)-releasing mechanism in liver nuclei.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Yamaguchi Mdoi
10.1016/0006-2952(93)90180-5subject
Has Abstractpub_date
1993-02-24 00:00:00pages
943-8issue
4eissn
0006-2952issn
1873-2968pii
0006-2952(93)90180-5journal_volume
45pub_type
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pub_type: 杂志文章
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