当前位置： SCI文献检索 > EUROPEAN JOURNAL OF ENDOCRINOLOGY期刊下所有文献 > Intracellular depletion of insulin: a comparative study with palmitate, oleate and elaidate in INS-1 cells.

Intracellular depletion of insulin: a comparative study with palmitate, oleate and elaidate in INS-1 cells.

Abstract：

OBJECTIVE:Free fatty acids (FFAs) deplete the intracellular insulin stores of pancreatic beta-cells. It has been suggested that this results from a lipotoxic dysregulation of both insulin secretion and insulin synthesis. In the present study, this hypothesis was tested within a 12-h time-course by directly relating the FFA-induced loss of intracellular insulin to corresponding parameters of insulin secretion and de novo biosynthesis. Palmitate, cis-monoenic oleate and the trans-monoenic elaidate were employed as model FFAs to elucidate potentially different effects due to chain length and configuration. METHODS:INS-1 cells were incubated for 1, 4 or 12 h with 11.2 mmol/l glucose with 200 micromol/l palmitate, oleate or elaidate and compared with non-FFA-exposed controls with respect to content and secretion of immunoreactive insulin (IRI). Biosynthesis of insulin was monitored by pulse-labeling experiments and by Northern blot analysis. RESULTS:IRI content dropped by 50-60% after a short-term exposure with all FFAs employed (P< or =0.001). It tended to recover after 12 h of treatment with oleate and elaidate but not with palmitate. FFA treatment increased insulin secretion by 25% (P< or =0.05) which could not account quantitatively for the intracellular loss. FFA-induced changes in insulin biosynthesis did not correlate clearly with the FFA-induced intracellular loss. CONCLUSIONS:The FFA-induced loss of IRI is an acute effect independent of the FFA employed. It cannot be sufficiently explained by FFA-induced perturbances of IRI secretion and biosynthesis. We therefore postulate an additional FFA-triggered mechanism, e.g. intracellular IRI degradation.

journal_name

Eur J Endocrinol

journal_title

European journal of endocrinology

authors

Bollheimer LC,Kemptner DM,Kagerbauer SM,Kestler TM,Wrede CE,Buettner R

doi

10.1530/eje.0.1480481

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

481-6

issue

4

eissn

0804-4643

issn

1479-683X

journal_volume

148

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF ENDOCRINOLOGY文献大全
  • DNA methylation profiling in MEN1-related pancreatic neuroendocrine tumors reveals a potential epigenetic target for treatment.

    abstract：OBJECTIVE:Epigenetic changes contribute to pancreatic neuroendocrine tumor (PanNET) development. Hypermethylation of promoter DNA as a cause of tumor suppressor gene silencing is a well-established oncogenic mechanism that is potentially reversible and therefore an interesting therapeutic target. Multiple endocrine neo...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-18-0195

    authors： Conemans EB,Lodewijk L,Moelans CB,Offerhaus GJA,Pieterman CRC,Morsink FH,Dekkers OM,de Herder WW,Hermus AR,van der Horst-Schrivers AN,Drent ML,Bisschop PH,Havekes B,Brosens LAA,Dreijerink KMA,Borel Rinkes IHM,Timmers HTM

    更新日期：2018-09-01 00:00:00

  • Calcium mobilization is a prerequisite for the expression of phorbol ester-stimulated luteinizing hormone secretion from pituitaries of male and acutely ovariectomized rats.

    abstract：:Ionomycin, which mobilizes Ca2+, and phorbol 12-myristate 13-acetate (PMA), which activates protein kinase C (PKC), were used to compare the effects/interactions of Ca2+ and PKC on luteinizing hormone (LH) secretion from pituitaries of intact male and acutely ovariectomized (72 h) rats. Quartered pituitaries from dono...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1300151

    authors： Das S,Fahmy NW,Bourne GA

    更新日期：1994-02-01 00:00:00

  • Catecholamine production in patients with gastroenteropancreatic neuroendocrine tumors.

    abstract：OBJECTIVE:Amine precursor uptake and decarboxylation is a classical feature of gastroenteropancreatic (GEP) neuroendocrine tumors (NET). Production of catecholamines was studied in GEP NET and non-NET patients. DESIGN:A cross-sectional study was undertaken. METHODS:We studied catecholamine and metabolite secretion in...

    journal_title：European journal of endocrinology

    pub_type： 临床试验,杂志文章

    doi：10.1530/eje.0.1400434

    authors： Ciofu A,Baudin E,Chanson P,Cailleux AF,Comoy E,Sabourin JC,Ducreux M,Schaison G,Schlumberger M

    更新日期：1999-05-01 00:00:00

  • Inhibin B plasma concentrations in infertile patients with DAZ gene deletions treated with FSH.

    abstract：OBJECTIVE:The DAZ (deleted in azoospermia) gene family on the Y chromosome long arm is the major candidate for the AZFc (azoospermia factor c) phenotype of male infertility and it is expressed only in germ cells. The aim of the study was to assess Sertoli cell function in subjects with AZFc deletions. DESIGN:Case-cont...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1460801

    authors： Foresta C,Bettella A,Moro E,Rossato M,Merico M,Garolla A,Ferlin A

    更新日期：2002-06-01 00:00:00

  • Safety and convenience of once-weekly somapacitan in adult GH deficiency: a 26-week randomized, controlled trial.

    abstract：OBJECTIVE:Somapacitan is a reversible albumin-binding growth hormone (GH) derivative, developed for once-weekly administration. This study aimed to evaluate the safety of once-weekly somapacitan vs once-daily Norditropin®. Local tolerability and treatment satisfaction were also assessed. DESIGN:26-week randomized, con...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1530/EJE-17-1073

    authors： Johannsson G,Feldt-Rasmussen U,Håkonsson IH,Biering H,Rodien P,Tahara S,Toogood A,Rasmussen MH,REAL 2 Study Group.

    更新日期：2018-05-01 00:00:00

  • Identification and functional characterization of loss-of-function mutations of the calcium-sensing receptor in four Italian kindreds with familial hypocalciuric hypercalcemia.

    abstract：OBJECTIVE:Identification and characterization of calcium-sensing receptor (CASR) mutations in four unrelated Italian kindreds with familial hypocalciuric hypercalcemia. DESIGN:Clinical evaluation and genetic analysis of CASR gene. Functional characterization of mutated CASRs. METHODS:Direct sequencing of CASR gene in...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-08-0798

    authors： Cetani F,Lemmi M,Cervia D,Borsari S,Cianferotti L,Pardi E,Ambrogini E,Banti C,Brown EM,Bagnoli P,Pinchera A,Marcocci C

    更新日期：2009-03-01 00:00:00

  • Health-related quality of life after successful surgery for primary hyperparathyroidism: no additive effect from vitamin D supplementation: results of a double-blind randomized study.

    abstract：OBJECTIVE:Vitamin D insufficiency is common in primary hyperparathyroidism (pHPT). Patients with pHPT frequently have a reduced health-related quality of life (HRQoL). Our objectives were to evaluate whether HRQoL in pHPT is associated with vitamin D insufficiency and whether vitamin D supplementation after parathyroid...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,随机对照试验

    doi：10.1530/EJE-14-0757

    authors： Åberg V,Norenstedt S,Zedenius J,Sääf M,Nordenström J,Pernow Y,Nilsson IL

    更新日期：2015-02-01 00:00:00

  • Thirst perception and arginine vasopressin production in a kindred with an activating mutation of the type 2 vasopressin receptor: the pathophysiology of nephrogenic syndrome of inappropriate antidiuresis.

    abstract：BACKGROUND:Activating mutations of the vasopressin receptor gene on the X chromosome cause the nephrogenic syndrome of inappropriate antidiuresis (NSIAD). We describe a male child who presented with persistent hyponatraemia and whose mother was also found to be hyponatraemic. She had learnt to avoid excess fluid consum...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-09-0246

    authors： Gupta S,Cheetham TD,Lambert HJ,Roberts C,Bourn D,Coulthard MG,Ball SG

    更新日期：2009-09-01 00:00:00

  • A novel HESX1 splice mutation causes isolated GH deficiency by interfering with mRNA processing.

    abstract：OBJECTIVE:Mutations in HESX1 represent a rare cause of GH deficiency (GHD) associated with a broad spectrum of other anomalies. We searched for causative mutations in a cohort of 244 Italian patients affected by combined and isolated GHD (IGHD). METHODS:The HESX1 gene-coding region and exon-intron boundaries were scre...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-11-0047

    authors： Vivenza D,Godi M,Faienza MF,Mellone S,Moia S,Rapa A,Petri A,Bellone S,Riccomagno S,Cavallo L,Giordano M,Bona G

    更新日期：2011-05-01 00:00:00

  • Growth modelling of non-functioning pituitary adenomas in patients referred for surgery.

    abstract：OBJECTIVE:Recent observational studies have established progression and recurrence rates of pituitary adenomas. However, it is still unknown how individual pituitary adenomas grow over years and whether growth kinetics follow a distinct growth model. The objective of this study was to define a growth model for non-func...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-07-0502

    authors： Honegger J,Zimmermann S,Psaras T,Petrick M,Mittelbronn M,Ernemann U,Reincke M,Dietz K

    更新日期：2008-03-01 00:00:00

  • Sleep-wake and melatonin pattern in craniopharyngioma patients.

    abstract：OBJECTIVE:To assess the influence of craniopharyngioma or consequent surgery on melatonin secretion, and the association with fatigue, sleepiness, sleep pattern and sleep quality. DESIGN:Cross-sectional study. METHODS:A total of 15 craniopharyngioma patients were individually matched to healthy controls. In this stud...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-13-1025

    authors： Pickering L,Jennum P,Gammeltoft S,Poulsgaard L,Feldt-Rasmussen U,Klose M

    更新日期：2014-06-01 00:00:00

  • Pheochromocytoma and pregnancy: a deceptive connection.

    abstract：:A pheochromocytoma in a pregnant patient is one of the most threatening medical conditions for mother, fetus, and physician. Although extraordinarily rare with a frequency of 0.002% of all pregnancies, this tumor is notorious for its devastating consequences. As in non-pregnant patients, the signs and symptoms are qui...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/EJE-11-0528

    authors： Lenders JW

    更新日期：2012-02-01 00:00:00

  • Neutrophil:lymphocyte ratio is positively related to type 2 diabetes in a large-scale adult population: a Tianjin Chronic Low-Grade Systemic Inflammation and Health cohort study.

    abstract：AIM:It is widely known that inflammation is related to type 2 diabetes (T2D), but few studies have shown a direct relationship between the immune system and T2D using a reliable biomarker. Neutrophil:lymphocyte ratio (NLR) is an easy-to-analyze inflammation biomarker, but few studies have assessed the relationship betw...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,随机对照试验

    doi：10.1530/EJE-15-0176

    authors： Guo X,Zhang S,Zhang Q,Liu L,Wu H,Du H,Shi H,Wang C,Xia Y,Liu X,Li C,Sun S,Wang X,Zhou M,Huang G,Jia Q,Zhao H,Song K,Niu K

    更新日期：2015-08-01 00:00:00

  • Coadministration of lanreotide Autogel and pegvisomant normalizes IGF1 levels and is well tolerated in patients with acromegaly partially controlled by somatostatin analogs alone.

    abstract：OBJECTIVE:To evaluate the efficacy and safety of coadministered lanreotide Autogel (LA; 120  mg/month) and pegvisomant (40-120  mg/week) in acromegaly. DESIGN:This is a 28-week, multicenter, open-label, single-arm sequential study. METHODS:Patients (n=92) biochemically uncontrolled, on somatostatin analogs (SSAs) or ...

    journal_title：European journal of endocrinology

    pub_type： 临床试验,杂志文章

    doi：10.1530/EJE-10-0867

    authors： van der Lely AJ,Bernabeu I,Cap J,Caron P,Colao A,Marek J,Neggers S,Birman P

    更新日期：2011-03-01 00:00:00

  • Monocentric study of 112 consecutive patients with childhood onset GH deficiency around and after transition.

    abstract：OBJECTIVES:Our aim was to analyze a large cohort of childhood onset GH deficiency (CO-GHD) adults from a unique adult center, in order to analyze their clinical management and to study the metabolic and bone status in relation to GHD and to the other pituitary deficits, and to evaluate these parameters during the long-...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-13-0572

    authors： Courtillot C,Baudoin R,Du Souich T,Saatdjian L,Tejedor I,Pinto G,Léger J,Polak M,Golmard JL,Touraine P,Transition GHD Group.

    更新日期：2013-10-01 00:00:00

  • Endocrine control of testicular somatic and premeiotic germ cell development in the immature testis of the primate Macaca mulatta.

    abstract：:Four groups(N = 3 per group) of juvenile rhesus monkeys (Macaca mulatta, 14-20 months old) received either vehicle or highly purified human follicle-stimulating hormone (FSH; 10 IU kg-1 day-1), human chorionic gonadotropin (hCG; 250 IU every alternate day) or both hormones for a period of 4 weeks. Testicular volume an...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1330235

    authors： Schlatt S,Arslan M,Weinbauer GF,Behre HM,Nieschlag E

    更新日期：1995-08-01 00:00:00

  • Tumor necrosis factor-alpha decreases thyrotropin-induced 5'-deiodinase activity in FRTL-5 thyroid cells.

    abstract：:Tumor necrosis factor-alpha (TNF-alpha) exerts various effects on many cell types. Acute administration of TNF-alpha to rats decrease hepatic 5'-deiodinase activity (5'D-I) and TNF-alpha has been implicated in the pathogenesis of the low triiodothyronine syndrome in non-thyroidal illness in humans. The thyroid, liver ...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1300502

    authors： Ongphiphadhanakul B,Fang SL,Tang KT,Patwardhan NA,Braverman LE

    更新日期：1994-05-01 00:00:00

  • Bone resorption in healthy and osteoporotic postmenopausal women: comparison markers for serum carboxy-terminal telopeptide of type I collagen and urinary pyridinium cross-links.

    abstract：:We compared two highly specific markers for bone resorption-pyridinium cross-links (pyridinoline (PYR) and deoxypyridinoline (DPR)) in urine and carboxy-terminal telopeptide of type I collagen (ICTP) in serum-in 63 healthy postmenopausal women and 63 women with osteoporosis characterized by more bone resorption than b...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1310258

    authors： Välimäki MJ,Tähtelä R,Jones JD,Peterson JM,Riggs BL

    更新日期：1994-09-01 00:00:00

  • Increased use of antimicrobial agents and hospital admission for infections in patients with primary adrenal insufficiency: a cohort study.

    abstract：BACKGROUND:Previous studies have suggested that infections are an important cause of death in patients with Addison's disease, but epidemiological studies on the frequency of infections in this population are lacking. OBJECTIVE:To assess and compare the incidence risk of infections in patients with primary adrenal ins...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-12-0879

    authors： Smans LC,Souverein PC,Leufkens HG,Hoepelman AI,Zelissen PM

    更新日期：2013-03-15 00:00:00

  • Skeletal muscle mitochondrial function in polycystic ovarian syndrome.

    abstract：OBJECTIVE:Polycystic ovarian syndrome (PCOS) is associated with skeletal muscle insulin resistance (IR), which has been linked to decreased mitochondrial function. We measured mitochondrial respiration in lean and obese women with and without PCOS using high-resolution respirometry. METHODS:Hyperinsulinemic-euglycemic...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-11-0419

    authors： Rabøl R,Svendsen PF,Skovbro M,Boushel R,Schjerling P,Nilas L,Madsbad S,Dela F

    更新日期：2011-10-01 00:00:00

  • Recent developments in the understanding of the pathophysiology of osteopetrosis.

    abstract：:Osteopetrosis is a rare metabolic bone disease characterized by a generalized increase in skeletal mass. It is inherited in a number of mammalian species, including man, and results from a congenital defect in the development or function of the osteoclasts. The consequent impairment of bone resorption prevents formati...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/eje.0.1340143

    authors： Felix R,Hofstetter W,Cecchini MG

    更新日期：1996-02-01 00:00:00

  • Growth hormone deficiency and replacement in hypopituitary patients previously treated for acromegaly or Cushing's disease.

    abstract：OBJECTIVE:To compare baseline characteristics in adult patients with growth hormone (GH) deficiency (GHD) who had previously been treated for Cushing's disease or acromegaly with data from patients with GHD of other aetiologies. To study the effects of GH therapy in those patients who had completed at least 6 months of...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1460067

    authors： Feldt-Rasmussen U,Abs R,Bengtsson BA,Bennmarker H,Bramnert M,Hernberg-Ståhl E,Monson JP,Westberg B,Wilton P,Wüster C,KIMS International Study Board of KIMS Study Group.

    更新日期：2002-01-01 00:00:00

  • Three-month sustained-release triptorelin (11.25 mg) in the treatment of central precocious puberty.

    abstract：OBJECTIVE:Depot GnRH agonists are commonly used in the treatment of central precocious puberty (CPP). The triptorelin 11.25 mg 3-month depot, currently used in adult indications, had not previously been evaluated in CPP. DESIGN:This was a multicenter, open-label, 12 month trial conducted in 64 CPP children (54 girls a...

    journal_title：European journal of endocrinology

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1530/eje.1.02056

    authors： Carel JC,Blumberg J,Seymour C,Adamsbaum C,Lahlou N,Triptorelin 3-month CPP Study Group.

    更新日期：2006-01-01 00:00:00

  • Opposite effects of thyroid hormones on binding proteins for steroid hormones (sex hormone-binding globulin and corticosteroid-binding globulin) in humans.

    abstract：:Sex hormone-binding globulin (SHBG) and corticosteroid-binding globulin (CBG) levels were evaluated in euthyroid (N = 111), hyper- (N = 58) and hypothyroid (N = 38) men, in pre- and postmenopausal women (study 1) and in hyper- (N = 24) and hypothyroid (N = 15) patients before and after treatment with carbimazole or le...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1320594

    authors： Dumoulin SC,Perret BP,Bennet AP,Caron PJ

    更新日期：1995-05-01 00:00:00

  • Chronic lymphocytic thyroiditis: could it be influenced by a petrochemical complex? Data from a cytological study in South-Eastern Sicily.

    abstract：INTRODUCTION:In genetically predisposed individuals, exogenous factors (including pollution) influence the development of Hashimoto's thyroiditis/chronic lymphocytic thyroiditis (CLT). CLT may also be a risk factor for associated thyroid cancer. Few data are available on the role of pollution from petrochemical complex...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/EJE-14-0864

    authors： Arena S,Latina A,Baratta R,Burgio G,Gullo D,Benvenga S

    更新日期：2015-04-01 00:00:00

  • Regulation of the sodium iodide symporter by iodide in FRTL-5 cells.

    abstract：OBJECTIVE:The acute decrease in iodide organification in the thyroid in response to excess iodide is termed the acute Wolff-Chaikoff effect and normal organification resumes in spite of continued high plasma iodide concentrations (escape from the acute Wolff-Chaikoff effect). We have recently reported that large doses ...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1440139

    authors： Eng PH,Cardona GR,Previti MC,Chin WW,Braverman LE

    更新日期：2001-02-01 00:00:00

  • Combined pyridostigmine-thyrotrophin-releasing hormone test for the evaluation of hypothalamic somatostatinergic activity in healthy normal men.

    abstract：:Pyridostigmine (PST), a cholinesterase inhibitor, induces a clear growth hormone (GH) release in man by suppression of hypothalamic somatostatin (SRIH). Somatostatin suppresses thyrotrophin (TSH) release in rats and men. Earlier studies showed that the thryotrophin-releasing hormone (TRH)-induced TSH response was not ...

    journal_title：European journal of endocrinology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1530/eje.0.1330457

    authors： Yang I,Woo J,Kim S,Kim J,Kim Y,Choi Y

    更新日期：1995-10-01 00:00:00

  • Expression of sex hormone-binding globulin mRNA in human ovarian cancers.

    abstract：:To know the role of sex hormone-binding globulin (SHBG) in the intracellular steroidal actions in human ovarian cancers, the expression of SHBG mRNA as a substitute for intracellular SHBG expression was investigated in normal ovarian tissues and ovarian tumors. In the present study, we used competitive reverse transcr...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1330327

    authors： Misao R,Nakanishi Y,Fujimoto J,Hori M,Ichigo S,Tamaya T

    更新日期：1995-09-01 00:00:00

  • Effect of activin on production and secretion of prolactin and growth hormone in cultured rat GH3 cells.

    abstract：OBJECTIVE:To evaluate the effect of the growth factor activin A on the secretion of prolactin (PRL) and GH in cultured GH3 cells. METHODS:The concentrations of PRL and GH secreted from GH3 cells cultured in media with and without activin A were measured by RIA, and the expression of PRL mRNA and GH mRNA were analyzed ...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章

    doi：10.1530/eje.0.1420506

    authors： Tamura N,Irahara M,Kuwahara A,Ushigoe K,Sugino H,Aono T

    更新日期：2000-05-01 00:00:00

  • Denosumab for bone diseases: translating bone biology into targeted therapy.

    abstract：:Signalling of receptor activator of nuclear factor-κB (RANK) ligand (RANKL) through RANK is a critical pathway to regulate the differentiation and activity of osteoclasts and, hence, a master regulator of bone resorption. Increased RANKL activity has been demonstrated in diseases characterised by excessive bone loss s...

    journal_title：European journal of endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/EJE-11-0454

    authors： Tsourdi E,Rachner TD,Rauner M,Hamann C,Hofbauer LC

    更新日期：2011-12-01 00:00:00