Abstract：
:The mechanisms underlying the hyper-radiosensitivity of AT cells were investigated by analyzing chromosome aberrations in the G(2) and M phases of the cell cycle using a combination of chemically induced premature chromosome condensation (PCC) and fluorescence in situ hybridization (FISH) with chromosome painting probes. Confluent cultures of normal fibroblast cells (AG1522) and fibroblast cells derived from an individual with AT (GM02052) were exposed to gamma rays and allowed to repair at 37 degrees C for 24 h. At doses that resulted in 10% survival, GM02052 cells were approximately five times more sensitive to gamma rays than AG1522 cells. For a given dose, GM02052 cells contained a much higher frequency of deletions and misrejoining than AG1522 cells. For both cell types, a good correlation was found between the percentage of aberrant cells and cell survival. The average number of color junctions, which represent the frequency of chromosome misrejoining, was also found to correlate well with survival. However, in a similar surviving population of GM02052 and AG1522 cells, induced by 1 Gy and 6 Gy, respectively, AG1522 cells contained four times more color junctions and half as many deletions as GM02052 cells. These results indicate that both repair deficiency and misrepair may be involved in the hyper-radiosensitivity of AT cells.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Kawata T,Ito H,George K,Wu H,Uno T,Isobe K,Cucinotta FAdoi
10.1667/0033-7587(2003)159[0597:rcaiat]2.0.co;2subject
Has Abstractpub_date
2003-05-01 00:00:00pages
597-603issue
5eissn
0033-7587issn
1938-5404journal_volume
159pub_type
杂志文章abstract：:Several studies over recent years have highlighted the possibility that radiation can induce transmissible genomic instability. Most of these involve in vitro irradiation and usually in vitro culture. Here it is reported that the short-half-life bone-seeking alpha-particle emitter (224)Ra did not induce excess transmi...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0345:lodtci]2.0.co;2
更新日期：2001-02-01 00:00:00
abstract：:In a patient with Morbus Hodgkin, structural aberrations of the chromosome type in peripheral lymphocytes were analyzed during radiation therapy (accumulated target dose 44.6 Gy: 22 fractions of 1.8 Gy each and 2 fractions of 2.5 Gy each at the end of the therapy). The blood was sampled about 5 min after a fraction an...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Wortmannin has been shown to be an efficient radiosensitizer. Since wortmannin is able to inhibit DNA-dependent protein kinase (DNA-PK) and double-strand break (DSB) rejoining, it is believed that its mechanism of radiation sensitization is through the inhibition of DNA-PK-mediated repair of DSBs. However, since wortm...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:Reverse-biased silicon p-n junction arrays using Silicon-On-Insulator technology have been proposed as microdosimeters. The performance of such detectors in boron neutron capture therapy (BNCT) is discussed. This work provides the first reported measurements using boron-coated silicon diode arrays as microdosimeters i...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
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更新日期：2011-06-01 00:00:00
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journal_title：Radiation research
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doi：10.1667/RR0535.1
更新日期：2006-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3336
更新日期：2005-04-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)155[0089:moakem]2.0.co;2
更新日期：2001-01-01 00:00:00
abstract：:Three somatic mutation assays were evaluated in men exposed to low-dose, whole-body, ionizing radiation. Blood samples were obtained between 1992 and 1999 from 625 Russian Chernobyl cleanup workers and 182 Russian controls. The assays were chromosome translocations in lymphocytes detected by FISH, hypoxanthine phospho...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)158[0424:tsgbac]2.0.co;2
更新日期：2002-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1745.1
更新日期：2009-08-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2007)168[183:HGUCTW]2.0.CO;2
更新日期：2007-08-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0626:qaovpo]2.0.co;2
更新日期：2002-06-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2517.1
更新日期：2011-08-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-10-01 00:00:00
abstract：:To determine the linear energy transfer (LET) dependence of the biological effects of densely ionizing radiation in relation to changes in the ionization density along the track, we measured the yields and spectrum of clustered DNA damages induced by charged particles of different atomic number but similar kinetic ene...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2093.1
更新日期：2010-08-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
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更新日期：2007-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3027
更新日期：2003-08-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:Extension of previous investigations at this laboratory regarding life shortening and tumor induction in the mouse has provided more complete dose-response information in the low dose region of X rays and neutrons. A complete observation of survival and late pathology has been carried out on over 2000 BC3F1 female mic...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-11-01 00:00:00
abstract：:A subline of a rat mammary carcinoma (MATB 13762), selected for resistance to melphalan, is cross-resistant to other alkylating drugs, to unrelated drugs and to ionizing radiation. The difference in radioresponse between the sensitive wild-type cell line and the melphalan- and radiation-resistant line (MLNr) is relate...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
