A correlation of argyrophilic nucleolar organizer regions with stages of hepatocellular carcinoma.

Abstract:

BACKGROUND:Recently, the number and morphologic features of argyrophilic nucleolar organizer regions (AgNOR), silver-stained nucleolar organizer regions, were thought to reflect the cellular proliferative activity and the grade of malignancy. In liver diseases, it has been reported that AgNOR scores for hepatocellular carcinomas (HCC) were significantly higher than those for benign and borderline lesions; the scores increased with histologic tumor grade. METHODS:Using the colloid silver staining technique to reveal AgNOR, 64 liver biopsy specimens with HCC were examined of which 14 had Stage I disease, 20 had Stage II, 14 had Stage III, 10 had Stage IVA, and 6 had Stage IVB (by International Union Against Cancer criteria). RESULTS:AgNOR in nuclei were divided into two types. Type 1 (T1-NOR) contained large and medium-sized brown dots with well-defined margins. Nucleoli were included in this type. Type 2 (T2-NOR) had fine black single dots and clusters without well-defined margins outside T1-NOR, but within the nucleus. The size and irregularity of T1-NOR changed with the progress of cancerous stages. The increase in the number of T2-NOR contributed preponderantly to the overall increase in Ag-NOR scores. CONCLUSIONS:These results suggest that HCC with smaller and/or irregular T1-NOR in combination with high T2-NOR scores have a more aggressive potential. AgNOR may be useful indicators for evaluating the progress of HCC.

journal_name

Cancer

journal_title

Cancer

authors

Shiro T,Seki T,Naitoh Y,Inoue K,Okamura A

doi

10.1002/1097-0142(19930101)71:1<44::aid-cncr282071

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

44-9

issue

1

eissn

0008-543X

issn

1097-0142

journal_volume

71

pub_type

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