Abstract：
:Differentiation of embryonic stem (ES) cells in vitro yields abundant hematopoietic progenitors, but achieving stable hematopoietic engraftment of irradiated mice has proven difficult, begging the question of whether ES cells give rise to hematopoietic stem cells in vitro, and limiting the application of ES cells as experimental and therapeutic models. We have employed a number of hematopoietic regulatory genes to probe the nature and developmental potential of ES-derived blood precursors. The chronic myeloid leukemia-associated BCR/ABL oncoprotein transforms a novel class of ES-derived embryonic hematopoietic stem cell that represents a common progenitor of primitive erythropoiesis and definitive lymphoid-myeloid blood development. Expression of the homeobox gene HoxB4 generated normal, non-leukemic hematopoietic progenitors that enabled long-term, multilineage hematopoietic engraftment in primary and secondary mouse recipients. We have used these repopulating hematopoietic stem cells to model therapeutic transplantation from ES cells. We treated an immunodeficient Rag2(-/-) mouse by therapeutic cloning, that is, isogenic ES cell generation by somatic cell nuclear transfer, gene correction, and cell replacement therapy. Comparable approaches with human ES cells are being developed to lay the foundation for cellular therapies in patients with a variety of bone marrow diseases.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Daley GQdoi
10.1111/j.1749-6632.2003.tb03240.xsubject
Has Abstractpub_date
2003-05-01 00:00:00pages
122-31eissn
0077-8923issn
1749-6632journal_volume
996pub_type
杂志文章,评审abstract：:We study the linear coupling between the Marangoni and Cowley-Rosensweig instabilities for a thin layer of ferrofluid subjected to a temperature gradient and a magnetic field. Both are perpendicular to the reference horizontal boundaries, one of which is a rigid plate, while the other is a free surface remaining flat ...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.2008.04064.x
更新日期：2009-04-01 00:00:00
abstract：:The clinical significance of a discovery of anti-histidyl-tRNA synthetase (Jo1) autoantibodies patients was established in the early diagnosis of antisynthetase syndrome (ASS) as the common form of this pathology is characterized by interstitial lung disease (ILD), inflammatory muscle disease, and production of anti-J...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1398.047
更新日期：2007-08-01 00:00:00
abstract：:Basal phagocytosis of neutrophils, a crucial component of non-specific immunity, is an eminently circadian parameter. In mice and rats, rates of phagocytosis peak in the second half of the dark span. A lipopolysaccharide-induced phagocytic response challenged in this period appears the most significant in amplitude an...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1356.032
更新日期：2005-12-01 00:00:00
abstract：:G3139 is an 18mer phosphorothioate oligonucleotide targeted to the initiation codon region of the Bcl-2 mRNA. Because of the ability of this antisense construct to downregulate the expression of Bcl-2 mRNA and protein, it has entered phase III clinical trials in a number of human cancers, including advanced melanoma. ...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1196/annals.1359.033
更新日期：2005-11-01 00:00:00
abstract：:Adolescence marks the beginning of adult-level cognitive control of behavior supported by the brain maturation processes of synaptic pruning and myelination. Cognitive development studies on adolescence indicate that this period is characterized by improvements in the performance of existing abilities including speed ...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1196/annals.1308.035
更新日期：2004-06-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) causes neuronal degeneration and, at a late stage, creates HIV-associated dementia (HAD) and other neurological abnormalities. Therefore, the need for neuroprotective agents is great. However, therapeutic agents that reduce HIV neurotoxicity are difficult to characterize and...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1196/annals.1403.010
更新日期：2007-12-01 00:00:00
abstract：:Evidence has been presented that lipoxygenase products of arachidonic acid metabolism released during the early stages of allergic reactions play an important role in the subsequent development of late bronchial responses. Moreover, because of this, the activity of the lipoxygenase pathway and sensitivity to the produ...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1988.tb38549.x
更新日期：1988-01-01 00:00:00
abstract：:The statistical techniques developed herein show strong promise as a nonparametric extension of the classical Wing-Kristofferson decomposition of variance in isochronous tapping to include tempo drift. It is now important to establish the relative role of the three sources of variance in discriminating among unselecte...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.2001.tb05753.x
更新日期：2001-06-01 00:00:00
abstract：:Indirect evidence, including neuroprotection against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-neurotoxicity by nitric oxide synthase (NOS) inhibitors and resistance of transgenic animals deficient in NOS, is controversial. We have reviewed evidence in favor of oxidative stress during the development of MPTP...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.2002.tb04083.x
更新日期：2002-05-01 00:00:00
abstract：:Frozen-hydrated microtubules have been studied by cryoelectron microscopy. We have described some structural features of microtubules, their relationship with the computed or X ray diffraction patterns, and the influence of the contrast transfer function. ...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.1986.tb34489.x
更新日期：1986-01-01 00:00:00
abstract：:The availability of computer-controlled artificial hearts, kidneys, and lungs, as well as the possibility of implanting human embryos in ex vivo uterus models or an artificial endometrium, presents new perspectives for creating an artificial uterus. Survival rates have also improved, with fetuses surviving from as ear...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.2011.05999.x
更新日期：2011-03-01 00:00:00
abstract：:Anaplasma phagocytophilum (A. phagocytophilum, formerly Ehrlichia phagocytophila) is a tick-borne pathogen responsible for tick-borne fever in ruminants, equine granulocytic ehrlichiosis (EGE) in horses, canine granulocytic ehrlichiosis (CGE) in dogs, and for human granulocytic ehrlichiosis (HGE). Human cases have bee...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1373.055
更新日期：2006-10-01 00:00:00
abstract：:Prodigiosin (PG) is an active component of bacterial origin, with reported apoptotic effects. We examined the activation of caspases-9, -8, and -3 in PG-treated Jurkat cells in a dose-response study. These caspases were activated in apoptotic cells, as judged by the appearance of cleavage products from procaspases, an...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.2002.tb04642.x
更新日期：2002-11-01 00:00:00
abstract：:3-Nitropropionic acid (3-NPA) is a model mitochondrial inhibitor that causes selective neurodegeneration in brain. 3-NPA-induced neurodegeneration occurs via a secondary neurotoxicity, caused initially by ATP depletion and redox changes in the cell. It is known that the hippocampal degeneration caused by mitochondrial...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1344.014
更新日期：2005-08-01 00:00:00
abstract：:This article reviews the implementation processes for interventions that integrate nutrition and psychosocial stimulation for children under 5 years of age in low- and middle-income countries. We examine the content of these programs, the delivery strategy, intensity and duration, personnel training and supervision, c...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/nyas.12313
更新日期：2014-01-01 00:00:00
abstract：:This review covers aspects of the cardiac mechanotransduction field at different levels, and advocates the possibility that mechanoelectro-chemical transduction forms part of a network of mechanically linked integration in heart-mechanically mediated integration (MMI). It assembles evidence and observations in the lit...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1196/annals.1380.022
更新日期：2006-10-01 00:00:00
abstract：:Clumps of corona cells were separated from oocytes at the moment of fertilization control and were subsequently processed and cut into 1-mu sections to study their general morphologic patterns. This study revealed an extensive phagocytosis of spermatozoa by corona cells. Clumps of mural granulosa cells of comparable s...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.1985.tb37534.x
更新日期：1985-01-01 00:00:00
abstract：:In this chapter, we examine the possibility that negative emotions contribute to the relationship between socioeconomic status (SES) and health. A model of the associations among SES, emotion, and health is presented first. We then review the evidence for this model, showing associations of SES with depression, hopele...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1999.tb08118.x
更新日期：1999-01-01 00:00:00
abstract：:In this experiment, we investigated the influence of the photoperiod upon androgen receptor (AR) distribution and steroid concentrations in ovaries of 21-day-old bank vole females. Sections (6 mum) were assayed immunohistochemically. ARs were localized in the nuclei of granulosa cells. The strongest immunoreaction was...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1327.050
更新日期：2005-04-01 00:00:00
abstract：:Much has been learned during the past decade about the specific retinoid-binding proteins that exist in plasma, and in the intracellular compartment in a number of tissues. Vitamin A is mobilized from liver stores and transported in plasma in the form of the lipid alcohol retinol, bound to a specific transport protein...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.1981.tb12738.x
更新日期：1981-02-27 00:00:00
abstract：:Previous studies have established that methanolic extracts of bovine brain and lung possess CDS activity with an agonist-like profile at alpha(2)-adrenoceptor binding sites (i.e., greater potency against [(3)H]-clonidine sites than against [(3)H]-RX-821001 sites). Following prolonged reflux in ethylacetate, monovalent...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1304.026
更新日期：2003-12-01 00:00:00
abstract：:Long-term changes in catecholamine levels and expression of their biosynthetic enzymes are associated with several stress-related disorders such as elevated plasma norepinephrine in posttraumatic stress disorder and increased postmortem tyrosine hydroxylase in the locus coeruleus with major depression. Stress elevates...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1314.010
更新日期：2004-12-01 00:00:00
abstract：:The effect of carcinogenesis on various hepatic microsomal parameters and related cell functions was studied in two tumor models. Hepatocarcinoma was produced by diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF) (Solt-Farber model) and mammary adenocarcinoma using R3230 AC cancer cell line. In these models th...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.1988.tb30145.x
更新日期：1988-01-01 00:00:00
abstract：:We found a novel genetic abnormality, heterozygous C-->T nucleotide substitution at position -1138 bp in the 5'-flanking region of the fibrinogen A alpha gene, in patients with hypofibrinogenemia. Luciferase reporter assay using the pGL3-basic vector and CHO cells indicates that the transcriptional activity of a vecto...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.2001.tb03539.x
更新日期：2001-01-01 00:00:00
abstract：:The autonomic nervous system consists of sympathetic, parasympathetic, and enteric ganglia and also of a specialized neuroendocrine derivative, the adrenomedullary chromaffin cells. ...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.2002.tb04524.x
更新日期：2002-10-01 00:00:00
abstract：:A four- to fivefold overexpression of the gene for the Alzheimer beta/A4 amyloid precursor protein (APP) in individuals with Down's Syndrome (DS) appears to be responsible for the fifty year earlier onset of Alzheimer's disease (AD) pathology in DS compared to the normal population. It is therefore likely that a dereg...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1993.tb23035.x
更新日期：1993-09-24 00:00:00
abstract：:The human organism is in a state of dynamic equilibrium, homeostasis. The stress system is activated when homeostasis is challenged by extrinsic or intrinsic forces, the stressors. This system, whose central component is the central nervous system (CNS) and includes corticotropin-releasing hormone (CRH) and noradrener...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1995.tb44666.x
更新日期：1995-12-29 00:00:00
abstract：:Effective emotion regulation may promote resilience and preschool classroom adjustment by supporting adaptive peer interactions and engagement in learning activities. We investigated how hypothalamus-pituitary-adrenal axis (HPA) regulation, cardiac reactivity, and classroom emotion displays related to adjustment among...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1196/annals.1376.043
更新日期：2006-12-01 00:00:00
abstract：:The number of physiologic and metabolic phenomena amenable to analysis using magnetic resonance (MR) techniques is increasing every year. MR techniques can now evaluate tissue parameters relevant to TCA cyclemetabolism, anerobic glycolysis, ATP levels, blood-brain barrier permeability, macrophage infiltration, cytotox...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1999.tb07828.x
更新日期：1999-01-01 00:00:00
abstract：:There are multiple connections from the entorhinal cortex (EC) to the hippocampus that carry the information from the EC to the hippocampus. Layer II cells of the medial EC innervating the dentate gyrus (DG)-molecular layer possess K(+)-outward currents and inward rectifier currents that are potentially modulated by c...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.2000.tb06722.x
更新日期：2000-06-01 00:00:00
