In vitro uptake and autoradiographic localization of tritiated gossypol in Taenia taeniaeformis metacestodes.

Abstract:

:Gossypol, a natural polyphenolic compound, induces growth-inhibitory and antiparasitic effects in Taenia taeniaeformis metacestodes in vivo and in vitro. We investigated the uptake and localization of [3H]-gossypol in this parasite. Metacestodes were incubated in 10(-5) M [3H]-gossypol at 37 degrees C. Parasites steadily took up tritium activity over the first 3 h of incubation, after which a plateau was maintained for the duration of the experiment. Tissue: medium radioactivity ratios revealed that intralarval tritium activity matched extralarval activity within 30 min of incubation and continued to increase with time. Reverse-phase high-performance liquid chromatographic (HPLC) analysis confirmed tissue incorporation of tritium activity that manifested as a single radioactive species. Autoradiography localized [3H]-gossypol to the tegument, calcareous corpuscles, and parenchyma over the first 2 h of incubation. By 6 h, parenchymal radioactivity had disappeared. T. taeniaeformis metacestodes rapidly take up and accumulate [3H]-gossypol in vitro. This accumulation is apparently selective for specific sites, which may have implications for gossypol's metacestocidal action.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Kulp SK,Rikihisa Y,Lin YC,Moh PP,Li PK,Gu Y

doi

10.1007/BF00932182

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

279-85

issue

4

eissn

0932-0113

issn

1432-1955

journal_volume

79

pub_type

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