Abstract:
:Photochemical decontamination (PCD) of platelet concentrates, with adequate preservation of platelet function, has been shown using 8-methoxypsoralen (8-MOP) and long wavelength UV light (UVA). To further evaluate this technique, models for the inactivation of pathogenic human cell-associated viruses and integrated proviral sequences are required. We have assessed the ability of the PCD technique to inactivate cell-associated human immunodeficiency virus 1 (HIV-1) in platelet concentrates. We correlated PCD inhibition of HIV-1 infectivity with 8-MOP-DNA adduct formation in contaminating nucleated cells, and measured the inhibition of polymerase chain reaction (PCR)-mediated amplification of cellular DNA sequences as a surrogate for inactivation of integrated proviral nucleic acid sequences. After PCD treatment (8-MOP 300 micrograms/mL, UVA 17 mW/cm2) for 60 minutes, 0.5 x 10(6) plaque-forming units (PFU)/mL of cell-associated HIV-1 were inactivated and no virus was detectable by infectivity assay. After 60 minutes of PCD, 15 8-MOP-DNA adducts per 1,000 bp were formed, while in the absence of UVA, no adducts were formed. PCR-mediated amplification of a 242-bp cellular DNA sequence (HLA-DQ-alpha) was inhibited when greater than eight psoralen-DNA adducts per 1,000 bp were present. These studies indicate that high titers of cell-associated HIV-1 in platelet concentrates were inactivated by PCD, and the numbers of 8-MOP-DNA adducts in nucleated cells were sufficient to inhibit amplification of DNA segments that encode for as few as 80 amino acids. Based on the frequency of 8-MOP-DNA adducts, for the 10-kb HIV-1 genome, the probability of an integrated genome without at least one 8-MOP adduct after 60 minutes of PCD was 10(-33).
journal_name
Bloodjournal_title
Bloodauthors
Lin L,Londe H,Hanson CV,Wiesehahn G,Isaacs S,Cimino G,Corash Lsubject
Has Abstractpub_date
1993-07-01 00:00:00pages
292-7issue
1eissn
0006-4971issn
1528-0020journal_volume
82pub_type
杂志文章相关文献
BLOOD文献大全abstract::Cutaneous T-cell lymphoma (CTCL) constitutes a malignant proliferative disease involving mostly CD4(+) T cells arising in the skin. Because of the lack of curative treatment options, interferons (IFN) have been introduced into the therapy of CTCL. Although effective even in advanced disease, response rates were about ...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:1998-11-15 00:00:00
abstract::E-cadherin gene is often termed a "metastasis suppressor" gene because the E-cadherin protein can suppress tumor cell invasion and metastasis. Inactivation of the E-cadherin gene occurs in undifferentiated solid tumors by both genetic and epigenetic mechanisms; however, the role of E-cadherin in hematologic malignanci...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-05-15 00:00:00
abstract::Fanconi anemia (FA) is an autosomal recessive syndrome featuring diverse symptoms including progressive bone marrow failure and early occurrence of acute myeloid leukemia. Nine genetic subtypes have been described for FA (A, B, C, D1, D2, E, F, G, and L), all of which have been connected to distinct disease genes, exc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-08-2915
更新日期:2004-04-01 00:00:00
abstract::Increased levels of endothelin-1 (Et-1), a potent vasoconstrictor, have been correlated with hypertension and neuronal damage in ischemic/reperfusion injury. The presence of polymorphonuclear cells (PMNs) in the brain has been shown to be directly responsible for this observed pathology. To address the question of whe...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::The anti-CD20 antibody rituximab depletes human B cells from peripheral blood, but it remains controversial to what extent tissue-resident B cells are affected. In representative patients with rheumatoid arthritis, we here demonstrate that recently activated presumably short-lived plasmablasts expressing HLA-DR(high) ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-266536
更新日期:2010-12-09 00:00:00
abstract::The high frequency of allogeneic reactive CD8(+) T cells in human and their resistance to immunosuppression might be one of the reasons why successful tolerance-inducing strategies in rodents have failed in primates. Studies on the requirement for T-helper cells in priming CD8(+) T-cell responses have led to disparate...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-02-268623
更新日期:2010-10-21 00:00:00
abstract::Anemia is a common clinical problem, and there is much interest in its role in promoting left ventricular hypertrophy through increasing cardiac workload. Normally, red blood cell production is adjusted through the regulation of erythropoietin (Epo) production by the kidney. One important cause of anemia is relative d...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-02-0605
更新日期:2002-10-01 00:00:00
abstract::Dendritic cells (DCs) are a key cell type in the initiation of the adaptive immune response. Recently, an additional role for DCs in suppressing myeloproliferation was discovered. Myeloproliferative disorder (MPD) was observed in murine studies with constitutive depletion of DCs, as well as in patients with congenital...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-05-850321
更新日期:2019-01-24 00:00:00
abstract::In this issue of Blood, Goussetis et al identify autophagy as a new pathway for the degradation of the oncoprotein BCR-ABL. They show that the therapeutic drug arsenic trioxide (AS(2)O(3)) targets BCR-ABL for autophagic degradation via a p62/SQSTM1-dependent mechanism that is critical for the antileukemic effect of th...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2012-08-451492
更新日期:2012-10-25 00:00:00
abstract::Clonal hematopoiesis (CH) in aplastic anemia (AA) has been closely linked to the evolution of late clonal disorders, including paroxysmal nocturnal hemoglobinuria and myelodysplastic syndromes (MDS)/acute myeloid leukemia (AML), which are common complications after successful immunosuppressive therapy (IST). With the ...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2016-01-636381
更新日期:2016-07-21 00:00:00
abstract::Endothelial cell prostacyclin (PGI2) inhibits platelet activation by raising platelet cyclic AMP. Previously, platelet activation was also shown to be blocked by plasmin formed by endothelium-derived tissue plasminogen activator (TPA). We have now studied interactions between PGI2 and plasmin in the control of platele...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::Pathologic blood clotting is a leading cause of morbidity and mortality in the developed world, underlying deep vein thrombosis, myocardial infarction, and stroke. Genetic predisposition to thrombosis is still poorly understood, and we hypothesize that there are many additional risk alleles and modifying factors remai...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-03-561027
更新日期:2014-07-03 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) is the major regulator of granulopoiesis and acts through binding to its specific receptor (G-CSF-R) on neutrophilic granulocytes. Previous studies of signaling from the 4 G-CSF-R cytoplasmic tyrosine residues used model cell lines that may have idiosyncratic, nonphysiolog...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.3.879
更新日期:2002-02-01 00:00:00
abstract::Adenosine deaminase (ADA) is an enzyme in the purine catabolic pathway that has been used as an enzymatic marker of T cell lymphoblastic malignancies due to its high specific activity in thymocytes and immature T cells. We have investigated whether the level of ADA activity in lymphoid leukemic cells correlates with t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::von Willebrand disease (VWD) is the most common autosomally inherited bleeding disorder. The disease represents a range of quantitative and qualitative pathologies of the adhesive glycoprotein von Willebrand factor (VWF). The pathogenic mechanisms responsible for the type 2 qualitative variants of VWF are now well cha...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2013-06-498303
更新日期:2013-11-28 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) lymphocytes manifest anomalous motility and cap formation. Since these processes involve cytoskeletal proteins, vimentin from intermediate filaments of normal and CLL lymphocytes was investigated using hetero- and monoclonal antisera. The antisera reacted predominantly with a 60-kD p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::Accumulating evidence has designated B cells as central players in the pathogenesis of immune diseases. In the late 1990s, anti-CD20 monoclonal antibodies were developed for the treatment of B-cell non-Hodgkin lymphomas, offering the opportunity to efficiently deplete the B-cell compartment for therapeutic immunointer...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2010-01-266668
更新日期:2010-07-29 00:00:00
abstract::Monoclonal antibodies were raised after injecting mice with isolated human dense granules. Several of these monoclonals were found to recognize a 40-Kd dense granule membrane protein. Western blot and immunofluorescent analysis confirmed the dense-granule specificity. After thrombin activation, the protein was found i...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Antiidiotype (Id) antibodies identify unique determinants within the surface immunoglobulin (Ig) that are present on B-cell tumors. Anti-Ids have been used for diagnosis and therapy of B-cell lymphoma and leukemia. A panel of 29 anti-Id monoclonal antibodies (MoAbs) that recognize shared idiotypes (SIds) on B-cell lym...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::We designed a phase 1-2 study to evaluate the safety and the efficacy of increasing doses of bendamustine (160 mg/m², 180 mg/m², and 200 mg/m² given on days -7 and -6) coupled with fixed doses of etoposide, cytarabine, and melphalan (BeEAM regimen) as the conditioning regimen to autologous stem cell transplantation fo...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2011-04-351924
更新日期:2011-09-22 00:00:00
abstract::Five novel monoclonal antibodies (mAbs; p0p 1-5) were used to characterize the structural and functional properties and the in vivo expression of the murine GPIb-IX complex (von Willebrand factor receptor). The molecular weights of the subunits are similar to the human homologs: GPIbalpha (150 kd), GPIbbeta (25 kd), a...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Hypomethylating agents (HMAs) improve survival in patients with higher-risk myelodysplastic syndromes (MDS) but are less well-studied in lower-risk disease. We compared the safety and efficacy of low-dose decitabine vs low-dose azacitidine in this group of patients. Adults with low- or intermediate 1-risk MDS or MDS/m...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood-2017-06-788497
更新日期:2017-09-28 00:00:00
abstract::CD44 is a widely distributed cell surface glycoprotein whose principal ligand has been identified as hyaluronic acid (HA), a major component of the extracellular matrix (ECM). Recent studies have demonstrated that activation through CD44 leads to induction of effector function in T cells and macrophages. In the curren...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-04-15 00:00:00
abstract::Analysis of fresh human tumors have indicated that patients with B type lymphoproliferative diseases and the majority of patients with acute lymphoblastic leukemia (ALL) express elevated levels of p53 production. It is suggested that in these human malignancies, p53 may provide a novel tool for monitoring cancer activ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract::The chemokine receptors (CCRs) CCR4 and CCR10, and the cutaneous lymphocyte antigen (CLA), have each been proposed as critical mediators of skin-specific TH lymphocyte homing in mice and humans. CLA initiates skin homing by mediating E-selectin-dependent tethering and rolling within cutaneous venules, but the specific...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-07-2348
更新日期:2003-03-01 00:00:00
abstract::Bone disease is one of the most debilitating manifestations of multiple myeloma. A complex interdependence exists between myeloma bone disease and tumor growth, creating a vicious circle of extensive bone destruction and myeloma progression. Proteasome inhibitors have recently been shown to promote bone formation in v...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2007-03-067710
更新日期:2007-08-15 00:00:00
abstract::Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired clonal hematologic disorder with multiple and varied clinical manifestations. The biochemical defect in PNH resides in the incomplete enzymatic assembly of glycosylphosphatidylinositol (GPI) anchors used for surface protein attachment. In all patients tested thu...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Interleukin-13 (IL-13) induced a potent mitogenic response in IL-3-dependent TF-1 cells and DNA synthesis to a lesser extent in MO7E and FDC-P1 cells. IL-13 stimulation of these lines, like IL-4 and insulin-like growth factor-1 (IGF-1), resulted in tyrosine phosphorylation of a 170-kD substrate. The tyrosine-phosphory...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::We genotyped 370 subjects with primary myelofibrosis (PMF) and 148 with postpolycythemia vera/postessential thrombocythemia (PPV/PET) MF for mutations of EZH2. Mutational status at diagnosis was correlated with hematologic parameters, clinical manifestations, and outcome. A total of 25 different EZH2 mutations were de...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-06-363424
更新日期:2011-11-10 00:00:00
abstract::We have previously determined that in African sickle cell anemia (SS) patients three different beta-like globin gene cluster haplotypes are associated with different percent G gamma (one of the two types of non-alpha chains comprising hemoglobin F [HbF]), mean percent HbF, and percent dense cells. We report now that i...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-03-15 00:00:00