Abstract:
:The collection of sputum for microbiological examination in young cystic fibrosis patients can be very difficult. However, a knowledge of bacterial flora colonizing the patient's airways is of paramount importance for proper antimicrobial therapy. It is also known that cystic fibrosis patients colonized by Pseudomonas species have a poorer prognosis than Pseudomonas-negative patients. Noninvasive ways of diagnosing airway inflammation that require only minimal cooperation of the patient might yield new possibilities for early detection of airway colonisation. The breath condensate method as a noninvasive diagnostic technique seems especially appropriate for use in children. Therefore, the aim of this study was to evaluate whether the breath condensate method could be used for detection of Pseudomonas species in children with cystic fibrosis. In total, 32 breath condensate and seven sputum samples were obtained from 13 cystic fibrosis patients with Pseudomonas aeruginosa- or Burkholderia cepacia-positive sputum culture (20 samples were obtained during forced expiration). PCR for combined detection of Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Burkholderia cepacia was performed. PCR results of all breath condensate samples were negative for Pseudomonas aeruginosa, Stenotrophomonas maltophilia, or Burkholderia cepacia, while all sputum sample results were positive. A minimum DNA quantity of 10 fg could be detected in dilution series of the positive control group. We conclude that the breath condensate method cannot be used as a tool for detection of Pseudomonas species.
journal_name
Pediatr Pulmonoljournal_title
Pediatric pulmonologyauthors
Vogelberg C,Hirsch T,Rösen-Wolff A,Kerkmann ML,Leupold Wdoi
10.1002/ppul.10352subject
Has Abstractpub_date
2003-10-01 00:00:00pages
348-52issue
4eissn
8755-6863issn
1099-0496journal_volume
36pub_type
杂志文章abstract::We compared proliferation (growth) and differentiation (development) related proteins in normal and hypoplastic fetal murine lungs. The hypoplastic lungs were created in CD-1 fetal mice by nitrofen exposure (25 mg per pregnant mouse given intragastrically on gestational day 8 [Gd8]), as published earlier. The lungs we...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1099
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVE:Among older children, sleep-disordered breathing (SDB) is associated with measurable neurocognitive consequences. However, diagnostic SDB thresholds are lacking for infants < 12 months. We sought to evaluate the relationship between SDB indices, gestational age (GA), and postmenstrual age (PMA) for infants wh...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24354
更新日期:2019-08-01 00:00:00
abstract::In this article, we describe existing CF registries with a focus on US registry data collected through the CF Foundation Patient Registry (CFFPR) and the Epidemiologic Study of CF (ESCF); highlight what registries have taught us regarding epidemiology of CF; showcase the impact of registries on research and clinical c...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,评审
doi:10.1002/ppul.23790
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:The aim of this study was to investigate the association among a validated symptom-based questionnaire for asthma control in children (CAN), forced expiratory volume in 1 sec (FEV(1)), and fractional exhaled nitric oxide (FE(NO)). METHODS:Observational cross-sectional study was performed in a consecutive sa...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.21144
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND:Acquiring sputum cultures from infants is considered challenging. We describe their yield in infants with cystic fibrosis (CF) and other chronic suppurative lung diseases (CSLDs). METHODS:Retrospective medical record review over a 4-year period, for infants aged 0-2 years with ≥2 airway bacterial cultures a...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.25103
更新日期:2020-12-01 00:00:00
abstract::We determined the effects of an immediately antecedent viral lower respiratory tract infection (LRI) on the severity of clinical illness, changes in lung function and airway histamine responsiveness produced by a subsequent LRI in 9-12 week old beagle puppies inoculated with canine adenovirus 2, followed in 2 weeks by...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1950100403
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:Interpregnancy interval (IPI) is defined as the period between a live birth and the conception of a subsequent fetus. Both short (IPI < 6 months) and long IPI (IPI > 60 months) have been shown to increase the risk for adverse perinatal outcomes, some of which, are known risk factors for obstructive sleep a...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.25240
更新日期:2020-12-31 00:00:00
abstract:OBJECTIVES:We assessed the performance of a tracheostomy decannulation protocol privileging safety over quickness, in pediatric patients undergoing rehabilitation from severe acquired brain injury. We analyzed factors associated with decannulation timing and possibility and examined cases of failure. HYPOTHESIS:A safe...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23832
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:Extremely low gestational age neonates (ELGANs) are at risk for pulmonary hypertension (PH). We hypothesized that PH, defined by echocardiogram at 36 weeks gestational age (GA), would associate with respiratory morbidity, increased oxidant stress, and reduced nitric oxide production. STUDY DESIGN:ELGANs in t...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23913
更新日期:2018-03-01 00:00:00
abstract::This study evaluates different ventilator strategies during gas (GV) and partial liquid ventilation (PLV) in spontaneously breathing animals. We hypothesized that during PLV, spontaneously breathing animals would self-regulate respiratory parameters by increasing respiratory rate (RR) and minute ventilation (V'E) when...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0496(199811)26:5<319::aid-ppul3
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND:Plastic bronchitis (PB) is a rare, variable, and potentially fatal disease. This study aimed to assess the efficacy of fiberoptic bronchoscopy (FOB) and bronchoalveolar lavage (BAL) in treating children with PB. METHODS:In total, 15 children with PB, between 2012 and 2020, were enrolled in our study. Within...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.25016
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:Multiple breath washout (MBW) is increasingly used in the clinical assessment of patients with cystic fibrosis (CF). Guidelines for MBW quality control (QC) were developed primarily for retrospective assessment and central overreading. We assessed whether real-time QC of MBW data during the measurement impro...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.25119
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVES:Our therapeutic approach to a habit/tic cough is simple reassurance in a single consultation. To quality assure our practice, we followed up children to determine outcomes at least 3 months after diagnosis. DESIGN:Consecutive children diagnosed over 6 years were studied. Medical records were analyzed retros...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23948
更新日期:2018-04-01 00:00:00
abstract::Most pneumatoceles disappear spontaneously and do not cause severe symptoms. Treatment alternatives include various conventional or surgical methods. However, an enlarging, complicated pneumatocele with cardiorespiratory instability requires imaging-guided catheter drainage or surgery. Here, we report the case of a ne...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23311
更新日期:2016-02-01 00:00:00
abstract::Phrenic nerve pacing has been used since 1966 to support breathing in quadriplegics and patients with central hypoventilation syndrome (CHS). Recently, using low-frequency, long-inspiratory-time (Ti) stimulation, phrenic nerve pacing has been used successfully to support breathing 24 hours per day in adults and older ...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1950040108
更新日期:1988-01-01 00:00:00
abstract::We enrolled 30 infants (median age 3 months, range 1-12 months), hospitalized for bronchiolitis in a randomized double-blind trial, to examine the efficacy and safety of nebulized epinephrine compared to salbutamol. Once admitted, patients were treated with either nebulized 0.5 mg of an 0.1% epinephrine solution (0.5 ...
journal_title:Pediatric pulmonology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/ppul.1040
更新日期:2001-04-01 00:00:00
abstract:AIM:Children with respiratory conditions benefit from care provided by pediatric pulmonologists. As these physicians are a small portion of the overall pediatric workforce, it is necessary to understand the practices and career plans of these specialists. METHODS:An internet survey was developed by the American Academ...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24253
更新日期:2019-04-01 00:00:00
abstract::SUMMARY. The detection of early airway disease in infants with cystic fibrosis (CF) may lead to earlier intervention and an improved prognosis. We hypothesized that the ratio of maximal expiratory flows while breathing a mixture of helium and oxygen (heliox) and air, referred to as density dependence (DD), would ident...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/1099-0496(200101)31:1<17::aid-ppul1002>3.0
更新日期:2001-01-01 00:00:00
abstract:OBJECTIVE:To define the mortality and long-term outcomes of children undergoing tracheostomy. DESIGN:Retrospective chart and Texas Department of Health Bureau of Vital Statistics review of patients admitted to a Pediatric Intensive Care Unit who underwent a tracheostomy between 2001 and 2011. Mortality and decannulati...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23657
更新日期:2017-07-01 00:00:00
abstract::The effect of viral infection on nasopharyngeal carriage of Streptococcus pneumoniae during childhood is not well known. We studied dynamics of pneumococcal colonization by quantitative PCR during the natural course of viral bronchiolitis. At time of admission, 47 (47%) of 100 patients with bronchiolitis carried pneum...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23390
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:Chest computerized tomography (CT) scores are associated with the frequency of future pulmonary exacerbations in people with cystic fibrosis (CF). However, cut-off values to identify children with mild lung disease with different risks for frequent future pulmonary exacerbations have not been identified. ME...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,随机对照试验
doi:10.1002/ppul.24144
更新日期:2018-10-01 00:00:00
abstract::Inadequate intake and suboptimal growth are common problems for patients with CF and a critical target for intervention. The purpose of this study was to compare the growth outcomes of children with CF who participated in a randomized clinical trial to improve energy intake and weight to children with CF receiving sta...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,随机对照试验
doi:10.1002/ppul.21322
更新日期:2011-01-01 00:00:00
abstract::Asthma has been found related to a higher risk of psychological problems. Inconsistent results have been reported with respect to the quality of life of children with asthma. So there may be a complex relationship between asthma severity, quality of life, and psychological factors. The aim of this study was to examine...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.20509
更新日期:2007-01-01 00:00:00
abstract::Lung hypoplasia is the main cause of congenital diaphragmatic hernia (CDH)-associated death but pathogenesis remains unclear. MiR-455-5p is involved in lung hypoplasia. We hypothesized that nitrofen causes abnormal miR-455-5p expression during lung development and designed this study to determine the relationship betw...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24739
更新日期:2020-06-01 00:00:00
abstract::We have studied the effect of triamcinolone acetonide (TAC) on airway morphogenesis of the Sprague-Dawley fetal rat in whole organ lung cultures from day 15 to day 21 of equivalent gestational age (6 days in culture). TAC produced an increased number of peripheral buds from day 18 onward and the airway and airspaces h...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1950140107
更新日期:1992-09-01 00:00:00
abstract::Twenty patients with cystic fibrosis (CF) were trained to mobilize intrabronchial secretions by a new method of positive expiratory pressure mask (PEP mask) physiotherapy (PT). Patients repeatedly expired forcefully through the PEP mask; expiratory resistance was varied by eight different internal diameter resistors a...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1950020608
更新日期:1986-11-01 00:00:00
abstract:BACKGROUND:Decreased lung function is common in preterm-born survivors. Increased fractional exhaled nitric oxide (FeNO) appears to be a reliable test for eosinophillic airway inflammation especially in asthma. We, systematically, reviewed the literature to compare FeNO levels in preterm-born children and adults who di...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,meta分析
doi:10.1002/ppul.24270
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Several studies have shown that exposure to air pollutants affects lung growth and development and can result in poor respiratory health in early life. METHODS:We included a subsample of 772 Mexican preschoolers whose mothers participated in a Prenatal Omega-3 fatty acid Supplements, GRowth, And Development...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24889
更新日期:2020-08-01 00:00:00
abstract::The raised lung volume technique is increasingly used to measure forced expiratory maneuvers in infants. However, there is no consensus regarding the optimal airway inflation pressure (P(inf)) required for such maneuvers, or the influence of small changes in P(inf) within and between infants. The aim of this study was...
journal_title:Pediatric pulmonology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/ppul.10060
更新日期:2002-02-01 00:00:00
abstract:BACKGROUND:Improper use of asthma inhalers is one of the potential factors of poor asthma control among children. This study aimed to assess the proper handling of asthma inhalers and asthma control in addition to factors influencing them among pediatric patients who self-administer their inhalers. METHODS:A cross-sec...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.25263
更新日期:2021-01-26 00:00:00