Telomere length and telomerase activity in the process of human fibroblast immortalization.

Abstract:

:To examine the telomere maintenance mechanism in the process of human fibroblast immortalization, we studied telomere length, telomerase activity, chromosome instability, and minisatellite alterations in human fibroblasts following the introduction of the human papillomavirus type 16 E6 gene or E7 gene, or both. One cell line immortalized by E6 alone maintained short telomeres, and its telomerase activity was positive. Fairly long and heterogeneous telomeres were maintained in all four E7-immortalized cell lines lacking telomerase activity. Of the three clones immortalized by both E6 and E7, two cell lines with telomerase activity maintained short telomeres, and the other cell line, which lacked telomerase activity, maintained long and heterogeneous telomeres. Although all immortal cell lines expressed mRNAs for human telomerase RNA component and telomerase-associated protein, expression of mRNA for human telomerase reverse transcriptase was detected only in the telomerase-positive cell lines. All immortal cell lines showed both chromosomal abnormalities, including structural and numerical changes, and minisatellite alterations detected by DNA fingerprinting. These findings indicate the existence of different telomere maintenance mechanisms in telomerase-positive and -negative fibroblast cell lines immortalized by E6, E7, or E6/ E7, and the possible involvement of chromosome instability and minisatellite alterations in the activation of the telomere maintenance mechanisms.

journal_name

Odontology

journal_title

Odontology

authors

Kumakura S,Yamamoto A,Yagisawa J,Uchida M,Tsutsui T

doi

10.1007/s102660200002

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

13-21

issue

1

eissn

1618-1247

issn

1618-1255

journal_volume

90

pub_type

杂志文章
  • Open flap versus flapless placement of dental implants. A randomized controlled pilot trial.

    abstract::The aim of this study was to compare the immediate postoperative period of participants rehabilitated with dental implants placed with a conventional technique or with a minimally invasive technique, without a mucoperiosteal flap elevation (flapless). Participants who needed implant placement were divided into two gro...

    journal_title:Odontology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s10266-018-0343-8

    authors: Jané-Salas E,Roselló-LLabrés X,Jané-Pallí E,Mishra S,Ayuso-Montero R,López-López J

    更新日期:2018-07-01 00:00:00

  • Effects of crown movement on periodontal biotype: a digital analysis.

    abstract::Orthodontic treatment has important correlations with periodontal changes. Various mechanical and biological factors are involved in determining such changes, but anatomical and morphological variables, generally addressed as periodontal biotype, play a key role. In measurement of such modifications, digital and non-c...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-018-0370-5

    authors: Lo Russo L,Zhurakivska K,Montaruli G,Salamini A,Gallo C,Troiano G,Ciavarella D

    更新日期:2018-10-01 00:00:00

  • Inter- and intra-observer agreement on Miller's classification of gingival tissue recessions.

    abstract::Miller's is the most commonly used classification of gingival tissue recessions, defined as the displacement of the soft tissue margin apical to the cemento-enamel junction. However, data on the reliability of this classification are missing so far, although reliability, which reflects the consistency of repeated meas...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-014-0179-9

    authors: Bertl K,Ruckenbauer D,Müller-Kern M,Durstberger G,Lettner S,Bruckmann C,Ulm C

    更新日期:2015-09-01 00:00:00

  • Effectiveness and SEM-EDX analysis following bleaching with an experimental bleaching gel containing titanium dioxide and/or chitosan.

    abstract::The aim of this study was to evaluate the bleaching effectiveness and mineral alterations following the use of experimental bleaching gels that included 6% hydrogen peroxide (HP), titanium dioxide (TiO2) and/or chitosan in comparison with the routinely used 35% HP bleaching gel. Thirty-six maxillary anterior teeth wer...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-020-00526-8

    authors: Sürmelioğlu D,Özçetin HK,Özdemir ZM,Yavuz SA,Aydın U

    更新日期:2021-01-01 00:00:00

  • In vitro cytotoxicity of metallic ions released from dental alloys.

    abstract::The cytotoxicity of a dental alloy depends on, but is not limited to, the extent of its corrosion behavior. Individual ions may have effects on cell viability that are different from metals interacting within the alloy structure. We aimed to investigate the cytotoxicity of individual metal ions in concentrations simil...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-014-0192-z

    authors: Milheiro A,Nozaki K,Kleverlaan CJ,Muris J,Miura H,Feilzer AJ

    更新日期:2016-05-01 00:00:00

  • Cyclophosphamide inhibits root development of molar teeth in growing mice.

    abstract::Root development of permanent teeth is disturbed in survivors of childhood cancer. Cyclophosphamide (CY) is a cytostatic drug commonly used for chemotherapy in children with cancer. This study aimed to evaluate the effects of CY on the development of molar teeth until the completion of occlusion in young mice, focusin...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-014-0158-1

    authors: Kawakami T,Nakamura Y,Karibe H

    更新日期:2015-05-01 00:00:00

  • Predictors of dental erosions in patients evaluated with upper digestive endoscopy: a cross-sectional study.

    abstract::The most studied medical condition related with dental erosions is gastroesophageal reflux disease (GERD). The aim of this study was to assess other predictors of dental erosions besides GERD in outpatients referred for upper digestive endoscopy. In a cross-sectional study, we prospectively evaluated 235 patients who ...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-020-00505-z

    authors: Rauber BF,Milani DC,Callegari-Jacques SM,Fornari L,Bonadeo NM,Fornari F

    更新日期:2020-10-01 00:00:00

  • Effect of filler size on wear resistance of resin cement.

    abstract::The purpose of this study was to evaluate the effect of filler size on the wear of resin cements. Materials tested included four experimental dual-cure resin cements (Kuraray) consisting of different-sized filler particles. A rectangular box cavity was prepared on the flattened occlusal surface of extracted human mola...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-001-8183-2

    authors: Shinkai K,Suzuki S,Katoh Y

    更新日期:2001-11-01 00:00:00

  • Comparison of the antibacterial activity of calcium silicate- and epoxy resin-based endodontic sealers against Enterococcus faecalis biofilms: a confocal laser-scanning microscopy analysis.

    abstract::This study assessed the antibacterial activity of BioRoot RCS in comparison with that of the Totalfill BC and AH Plus sealers against Enterococcus faecalis biofilms in dentinal tubules using confocal laser-scanning microscopy. Sixty-six root dentin halves were prepared and sterilized. Three sections were used to ensur...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-019-00425-7

    authors: Alsubait S,Albader S,Alajlan N,Alkhunaini N,Niazy A,Almahdy A

    更新日期:2019-10-01 00:00:00

  • Conditioning effects of cavities prepared with an Er,Cr:YSGG laser and an air-turbine.

    abstract::The purpose of this study was to examine, morphologically and histochemically, five types of conditioning effects on cavities prepared with an Er,Cr:YSGG laser and an air-turbine. Cavities were prepared using a Waterlase(®) MD turbo handpiece (W) and an air-turbine (AT) on human extracted molars. The cavity conditioni...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-011-0023-4

    authors: Kato C,Taira Y,Suzuki M,Shinkai K,Katoh Y

    更新日期:2012-07-01 00:00:00

  • Surface deformation of several nickel-titanium rotary instruments after removal of the root canal filling materials: a scanning electron microscope evaluation.

    abstract::The aim of the present study was to investigate and compare the surface deformations of different NiTi rotary files after retreatment under scanning electron microscope (SEM). A total of 240 upper central incisor teeth were obturated with vertical compaction technique and divided into 6 groups. Root canals were retrea...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-019-00454-2

    authors: Özyürek T,Demiryürek EÖ

    更新日期:2020-04-01 00:00:00

  • Magnetic resonance imaging findings of nodular fasciitis in the mental region.

    abstract::Nodular fasciitis (NF) is a benign reactive lesion of the soft tissues related to the fascia and characterized by fibroblastic proliferation. The most common site is the upper extremities (46%), followed by the head and neck region (20%). In the orofacial region, the lesion typically develops within the subcutaneous s...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-004-0042-5

    authors: Katada T,Tsuchimochi M,Oda T,Sasaki Y,Toyama M,Katagiri M

    更新日期:2004-09-01 00:00:00

  • Fluoride inhibits the response of bone cells to mechanical loading.

    abstract::The response of bone cells to mechanical loading is mediated by the cytoskeleton. Since the bone anabolic agent fluoride disrupts the cytoskeleton, we investigated whether fluoride affects the response of bone cells to mechanical loading, and whether this is cytoskeleton mediated. The mechano-response of osteoblasts w...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-011-0013-6

    authors: Willems HM,van den Heuvel EG,Castelein S,Buisman JK,Bronckers AL,Bakker AD,Klein-Nulend J

    更新日期:2011-07-01 00:00:00

  • The effect of iloprost on cell proliferation and angiogenesis-related gene expression in human periodontal ligament cells.

    abstract::Periodontal ligament is considered a rich source of mesenchymal stem cells for pulp regeneration. This study investigated the effect of iloprost, a prostacyclin analog, on cell proliferation and the expression of angiogenesis-related genes in human periodontal ligament cells (hPDLs) in vitro. The hPDLs were treated wi...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-017-0307-4

    authors: Jearanaiphaisarn T,Sanharati T,Pavasant P,Nakalekha Limjeerajarus C

    更新日期:2018-01-01 00:00:00

  • Immunocytochemical study of granular duct cells with a hormonally enhanced granular cell phenotype in the mouse parotid gland.

    abstract::In the parotid glands (PGs) of intact male mice (12 weeks of age, ICR strain), immunofluorescence labels for a true tissue kallikrein, mK1, and for nerve growth factor (NGF) were recognized through the subluminal edges of the striated duct (SD) segments and interlobular duct segments. Because of their small size, secr...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-008-0090-3

    authors: Kurabuchi S,Hosoi K

    更新日期:2009-01-01 00:00:00

  • In vivo imaging of enamel by reflectance confocal microscopy (RCM): non-invasive analysis of dental surface.

    abstract::The aim is to establish the feasibility to image in vivo microscopic dental surface by non-invasive, real-time, en face Reflectance Confocal Microscopy (RCM). Fifteen healthy volunteers referred at the Multidisciplinary Department of Medical-Surgical and Odontostomatological Specialties, Second University of Naples, N...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-013-0110-9

    authors: Contaldo M,Serpico R,Lucchese A

    更新日期:2014-07-01 00:00:00

  • Impact of Streptococcus mutans on the generation of fluorescence from artificially induced enamel and dentin carious lesions in vitro.

    abstract::The purpose of this study was to examine whether Streptococcus mutans is implicated in the generation of fluorescence detected in carious lesions. Enamel surfaces and dentin cavities of extracted human teeth were subjected to artificial caries generation by exposing them either to a culture medium containing S. mutans...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-007-0077-5

    authors: Shigetani Y,Takenaka S,Okamoto A,Abu-Bakr N,Iwaku M,Okiji T

    更新日期:2008-07-01 00:00:00

  • The effect of pulpotomy using a calcium-enriched mixture cement versus one-visit root canal therapy on postoperative pain relief in irreversible pulpitis: a randomized clinical trial.

    abstract::The purpose of this noninferiority trial was to compare postoperative pain relief after one-visit root canal therapy (ORCT) with a pulpotomy performed with a new endodontic calcium-enriched mixture cement (PCEM) in human permanent molars with irreversible pulpitis. A total of 407 selected patients were randomly alloca...

    journal_title:Odontology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1007/s10266-010-0127-2

    authors: Asgary S,Eghbal MJ

    更新日期:2010-07-01 00:00:00

  • A 5-year prospective clinical trial on short implants (6 mm) for single tooth replacement in the posterior maxilla: immediate versus delayed loading.

    abstract::The aim of this paper was to demonstrate the treatment outcomes following immediate functional loading concept of short implants inserted for single tooth replacement in the posterior maxilla. The study was performed on 63 patients who received short (6 mm) implants for single tooth replacement in the posterior maxill...

    journal_title:Odontology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s10266-018-0378-x

    authors: Ayna M,Wessing B,Gutwald R,Neff A,Ziebart T,Açil Y,Wiltfang J,Gülses A

    更新日期:2019-04-01 00:00:00

  • Periodontal disease and risk of atherosclerotic coronary heart disease.

    abstract::Atherosclerosis is an important component of coronary heart disease (CHD), which is the leading cause of death worldwide, including in Japan. Because atherosclerotic processes are typified by chronic inflammatory responses, which are similar to those elicited by chronic infection, the role of infection in promoting or...

    journal_title:Odontology

    pub_type: 杂志文章,评审

    doi:10.1007/s10266-009-0104-9

    authors: Nakajima T,Yamazaki K

    更新日期:2009-07-01 00:00:00

  • External versus internal abutment connection implants: a survey of opinions and decision making among experienced implant dentists in Japan.

    abstract::Internal connection implants have been attracting attention in recent years leading to their being adopted for many implant systems and an increase in market share over other connections. However, internal connection implants have only been used clinically for a short period of time resulting in few clinical studies i...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-013-0141-2

    authors: Hagiwara Y,Carr AB

    更新日期:2015-01-01 00:00:00

  • Evaluation of condylar cortical bone thickness in patient groups with different vertical facial dimensions using cone-beam computed tomography.

    abstract::The aim of this study is to evaluate through computed tomography differences in cortical plate thickness of condyle in patients with a different facial vertical skeletal pattern. The final sample of this retrospective study included CBCT exams of 60 adult subjects (mean age 33.2 ± 5.6), selected from the digital archi...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-020-00510-2

    authors: Lo Giudice A,Rustico L,Caprioglio A,Migliorati M,Nucera R

    更新日期:2020-10-01 00:00:00

  • Evaluation of chemerin and its receptors, ChemR23 and CCRL2, in gingival tissues with healthy and periodontitis.

    abstract::Chemerin is a chemoattractant protein that directs inflammatory cells that express its receptor chemokine receptor-like 1 (ChemR23) towards sites of inflammation. C-C chemokine receptor-like 2 (CCRL2), is the other receptor of chemerin, improves the interaction between chemerin and ChemR23. The aim of this study was t...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-017-0297-2

    authors: Özcan E,Saygun NI,Ilıkçı R,Karslıoğlu Y,Muşabak U,Yeşillik S

    更新日期:2018-01-01 00:00:00

  • The Stephan Curve revisited.

    abstract::The Stephan Curve has played a dominant role in caries research over the past several decades. What is so remarkable about the Stephan Curve is the plethora of interactions it illustrates and yet acid production remains the dominant focus. Using sophisticated technology, it is possible to measure pH changes in plaque;...

    journal_title:Odontology

    pub_type: 杂志文章,评审

    doi:10.1007/s10266-012-0092-z

    authors: Bowen WH

    更新日期:2013-01-01 00:00:00

  • Comparison of two curing protocols during adhesive cementation: can the step luting technique supersede the traditional one?

    abstract::This study aims to compare the degree of conversion of two different curing protocols used during adhesive cementation. The following resin luting agents were tested: Hri Flow (MF) and pre-heated Hri Micerium (MH); light-cure Nexus Third Generation (NX3L) and dual-cure Nexus Third Generation (NX3D); dual cured RelyX U...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-020-00558-0

    authors: Tosco V,Monterubbianesi R,Orilisi G,Sabbatini S,Conti C,Özcan M,Putignano A,Orsini G

    更新日期:2020-10-31 00:00:00

  • Effect of self-etch adhesives on the internal adaptation of composite restoration: a CP-OCT Study.

    abstract::Despite improvements in dental adhesive materials, internal adaptation remains a challenge in bonded restorations. The aim of this study was to compare microgaps and internal floor adaptation between two different self-etch adhesives in class-V cavities using cross-polarization optical coherence tomography (CP-OCT). I...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-018-0381-2

    authors: Bakhsh TA,Altouki NH,Baeesa LS,Baamer RA,Alshebany RM,Natto Z,Nasir A,Turkistani A,Hasanain F,Naguib GH

    更新日期:2019-04-01 00:00:00

  • Tripartite motif 31 alleviates IL-1ß secretion via promoting the ubiquitination of pyrin domain domains-containing protein 3 in human periodontal ligament fibroblasts.

    abstract::Apical periodontitis (AP) is an inflammatory disease caused by bacteria infection and is regarded as a common disease in the world. In the progression of AP, the function of nucleotide-binding oligomerization, leucine-rich repeat and pyrin domain domains-containing protein 3 (NLRP3) inflammasome has been revealed. Alt...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-020-00519-7

    authors: Wu X,Lu M,Ding S,Zhong Q

    更新日期:2020-07-01 00:00:00

  • Demineralized enamel reduces margin integrity of self-etch, but not of etch-and-rinse bonded composite restorations.

    abstract::The aim of this study was to investigate margin integrity of Class V composite restorations in demineralized and sound enamel after bonding with different etch-and-rinse and self-etch adhesive systems. Out of a total of 60 specimens from bovine incisors, 30 specimens were demineralized (21 days, acid buffer, pH 4.95) ...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-018-0398-6

    authors: Körner P,Sulejmani A,Wiedemeier DB,Attin T,Tauböck TT

    更新日期:2019-07-01 00:00:00

  • The effect of tooth bleaching on the bond strength of an experimental primer to enamel.

    abstract::This study evaluated the effect of tooth bleaching on the microtensile bond strength (microTBS) of an experimental primer to enamel. Materials used were an experimental tooth manicure system (Shofu) composed of primer and light-cured flowable resin composite. Flattened enamel surfaces of bovine teeth were bleached wit...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-007-0075-7

    authors: Shinkai K,Wakaki S,Suzuki S,Katoh Y

    更新日期:2007-07-01 00:00:00

  • Expression of keratin 18 in the periderm cells of the lingual epithelium of fetal rats: visualization by fluorescence immunohistochemistry and differential interference contrast microscopy.

    abstract::We examined the expression of keratin 18 (K18), by immunofluorescence staining, while monitoring morphological changes in the periderm on the lingual epithelium of rats by laser-scanning microscopy of epoxy resin-embedded, semi-ultrathin sections. We also examined differential interference contrast (DIC) images of the...

    journal_title:Odontology

    pub_type: 杂志文章

    doi:10.1007/s10266-005-0059-4

    authors: Iwasaki S,Aoyagi H,Asami T

    更新日期:2006-09-01 00:00:00