Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparison of the assay with ELISA.

abstract：:A series of already published and unpublished seroepidemiological surveys for toxoplasmosis, carried out in Chile in 1982-1994, is reviewed, expanded and analyzed. The surveys included 76,317 apparently healthy individuals of different ages (0.57% of the country's total population), from 309 urban and rural-periurban ...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Contreras M,Schenone H,Salinas P,Sandoval L,Rojas A,Villarroel F,Solis F

更新日期：1996-11-01 00:00:00

abstract：:This study evaluated the whole blood immunochromatographic card test (ICT card test) in a survey performed in Northeastern Brazil. 625 people were examined by the thick blood film (TBF) and ICT card test. Residents of a non-endemic area were also tested by the whole blood card test and Og4C3. The sensitivity of the IC...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Braga C,Dourado MI,Ximenes RA,Alves L,Brayner F,Rocha A,Alexander N

更新日期：2003-05-01 00:00:00

abstract：:In the last years, new techniques of neuroimages and histopathological methods have been added to the management of cerebral mass lesions in patients with AIDS. Stereotactic biopsy is necessary when after 14 days of empirical treatment for Toxoplasma gondii encephalitis there is no clinical or neuroradiologic improvem...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Corti ME,Yampolsky C,Metta H,Valerga M,Sevlever G,Capizzano A

更新日期：2004-07-01 00:00:00

abstract：:A 25-year-old male without prior co-morbidities was admitted to hospital with Fusobacterium necrophorum bacteremia, where he was found to have liver and splenic abscesses. Further evaluation with echocardiography revealed a bicuspid aortic valve with severe insufficiency and a 1.68 x 0.86 cm vegetation. The patient re...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Handler MZ,Miriovsky B,Gendelman HE,Sandkovsky U

更新日期：2011-05-01 00:00:00

abstract：:Exoantigens (exo) from Leptomonas seymouri and Crithidia fasciculata were used in an enzyme linked immunosorbent assay (ELISA), showing 100% reactivity with sera from visceral leishmaniasis (VL) cases, and no reactivity with American tegumentary leishmaniasis (ATL) ones. Our results have indicated that these exoantige...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Kesper N,Teixeira MM,Lindoso JA,Barbieri CL,Umezawa ES

更新日期：2017-04-03 00:00:00

abstract：:Candidemia is a significant cause of bloodstream infections (BSI) in nosocomial settings. The identification of species can potentially improve the quality of care and decrease human mortality. Quantitative PCR (qPCR) was evaluated for Candida albicans detection using culture suspensions containing C. albicans , spike...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Busser FD,Coelho VC,Fonseca CA,Del Negro GMB,Shikanai-Yasuda MA,Lopes MH,Magri MMC,Freitas VLT

更新日期：2020-02-07 00:00:00

abstract：:In 2018, Bhutan reported 54 cases of malaria, of which six were indigenous, 14 introduced and 34 imported. Considering the continuous reduction in the number of indigenous cases, Bhutan plans to eliminate malaria by 2025 under the Bhutan Malaria Elimination Strategy. The study was conducted to assess the presence of a...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Wangchuk S,Gyeltshen S,Dorji K,Wangdi T,Dukpa T,Namgay R,Dorjee S,Tobgay T,Chaijaroenkul W,Na-Bangchang K

更新日期：2019-09-12 00:00:00

abstract：:Early in 1995 the first case of Hantavirus Pulmonary Syndrome was serologically confirmed in El Bolsón (Province of Río Negro, Argentina), corresponding to the third outbreak reported in Argentina. A total of 26 cases of HPS related to the Andean region of Rio Negro Province, were reported from 1993 to 1996, 17 in El ...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Cantoni G,Lazaro M,Resa A,Arellano O,Amestoy AM,De Bunder S,Herrero E,Perez A,Larrieu E

更新日期：1997-07-01 00:00:00

abstract：:Haptoglobin assay, a highly sensitive method to detect intravascular hemolysis was carried out in the sera of 19 patients referred to Hospital Vital Brazil with the cutaneous form of loxoscelism in order to investigate the occurrence of mild intravascular hemolysis. Data from this series did not show decreased levels ...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Morena P,Nonoyama K,Cardoso JL,Barretto OC

更新日期：1994-03-01 00:00:00

abstract：:Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunof...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Costa-Cruz JM,Bullamah CB,Gonçalves-Pires Mdo R,Campos DM,Vieira MA

更新日期：1997-11-01 00:00:00

abstract：:A clinical trial pilot study, double-blinded, randomized, and controlled with a placebo to assess the effectiveness of oral doxycycline (200 mg, single dose) in preventing leptospirosis after high exposure to potentially contaminated water was performed in São Paulo, SP, Brazil. Confirmed cases were defined as those w...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Gonsalez CR,Casseb J,Monteiro FG,Paula-Neto JB,Fernandez RB,Silva MV,Camargo ED,Mairinque JM,Tavares LC

更新日期：1998-01-01 00:00:00

abstract：:In order to determine the prevalence of hepatitis C virus (HCV) infection in quilombo remnant communities in Central Brazil, 1,007 subjects were interviewed in all 12 communities existing in Mato Grosso do Sul State, Central Brazil. Blood samples were collected and sera were tested for anti-HCV by enzyme-linked immuno...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Reis NR,Motta-Castro AR,Silva AM,Teles SA,Yoshida CF,Martins RM

更新日期：2008-11-01 00:00:00

abstract：:The Mato Grosso do Sul State (MS) has the second-largest indigenous population and the highest incidence rates of TB among indigenous people in Brazil. However, little is known about the risk factors associated with active TB in indigenous people in the region, especially regarding socioeconomic factors. The aim of th...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Malacarne J,Kolte IV,Freitas LP,Orellana JDY,Souza MLP,Souza-Santos R,Basta PC

更新日期：2018-10-25 00:00:00

abstract：:The aim of the present work was to determine the prevalence of IgG and IgM anti-Toxoplasma gondii antibodies and the factors associated to the infection in pregnant women attended in Basic Health Units in Rolândia, Paraná, Brazil. The sample was divided in two groups: group I (320 pregnant women who were analyzed from...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Dias RC,Lopes-Mori FM,Mitsuka-Breganó R,Dias RA,Tokano DV,Reiche EM,Freire RL,Navarro IT

更新日期：2011-07-01 00:00:00

abstract：:A sedimentation technique is described, in which a fecal suspension is placed on top of an aqueous sucrose solution of specific gravity 1.015 g/cm3. Using 100 by 15 mm test tubes, duplicate gravity sedimentation experiments were made using homogenized fecal suspensions (single-columns) and fecal suspensions placed on ...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Moitinho Mda L,Ferreira CS

更新日期：1992-05-01 00:00:00

abstract：:Postsurgical acute suppurative parotitis is a bacterial gland infection that occurs from a few days up to some weeks after abdominal surgical procedures. In this study, the authors analyze the prevalence of this complication in Hospital das Clínicas/São Paulo University Medical School by prospectively reviewing the ch...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Belczak SQ,Cleva RD,Utiyama EM,Cecconello I,Rasslan S,Parreira JG

更新日期：2008-09-01 00:00:00

abstract：:Adrenal involvement by Paracoccidioides brasiliensis was described at necropsies and in many clinical studies, but only in adults. Therefore, the aim of this study was to evaluate adrenal function in children with paracoccidioidomycosis. Twenty-three children with the systemic form of paracoccidioidomycosis were evalu...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Pereira RM,Guerra-Júnior G,Tresoldi AT

更新日期：2006-11-01 00:00:00

abstract：:American Tegumentary Leishmaniasis (ATL) is endemic in the municipality of Montes Claros, Minas Gerais State. The use of geotechnology such as spatial statistics and remote sensing has contributed to a better understanding of the eco-epidemiology of diseases, and consequently a better definition of control strategies....

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Ursine RL,Rocha MF,Sousa JF,Santos RCD,Soares MD,Gusmão MSF,Leite ME,Vieira TM

更新日期：2021-01-20 00:00:00

abstract：:In the São Paulo State, Brazil, where the Biomphalaria tenagophila is the intermediate host, the Ribeira Valley is an important endemic schistosomiasis mansoni area. During last eleven years there has been intense control measures focusing on schistosomiasis. The efforts have been concentrated in the municipalities of...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Figueiredo AC,Dias LC,Marques EH

更新日期：1992-05-01 00:00:00

abstract：:The prevalence of thermotolerant Campylobacter in diarrhoeic and healthy children as well as in dogs, hens, ducks and pigeons was determined in Southern Chile. Campylobacter were found in 34.5% of the faecal samples examined. The isolation rate of Campylobacter in diarrhoeic and healthy children was 16.3% and 6.4% res...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Fernández H,Kahler K,Salazar R,Ríos MA

更新日期：1994-09-01 00:00:00

abstract：:Infection by Candida spp. is associated with high mortality rates, especially when treatment is not appropriate and/or not immediate. Therefore, it is necessary to correctly identify the genus and species of Candida. The aim of this study was to compare the identification of 89 samples of Candida spp. by the manual me...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Souza MN,Ortiz SO,Mello MM,Oliveira Fde M,Severo LC,Goebel CS

更新日期：2015-07-01 00:00:00

abstract：:Molluscs with shells very similar to Oncomelania nosophora were collected at Pedro Leopoldo and Lagoa Santa counties, MG, Brazil. They were identified, according to shell and soft part morphology, as Idiopyrgus souleyetianus. The others hidrobioids identified in Brazil are also listed. ...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Lima LC,de Souza CP

更新日期：1990-03-01 00:00:00

abstract：:To determine the larvicidal activity of various extracts of Gymnema sylvestre against the Japanese Encephalitis vector, Culex tritaeniorynchus in Tamilnadu, India. To identify the active principle present in the promising fraction obtained in Chlorofom:Methanol extract of Fraction 2. The G. sylvestre leaf extracts wer...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Elumalai K,Dhanasekaran S,Krishnappa K

更新日期：2012-12-01 00:00:00

abstract：:Field collections of immatures and adults of Culicidae were carried out in the Rio Doce Valley, Espírito Santo State, aiming to increase knowledge on the anopheline species of that region. Considering all Culicidae species collected, among other anopheline it is noteworthy the presence of Anopheles darlingi, An. oswal...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Natal D,Urbinatti PR,Malafronte Rdos S,Rezende HR,Cerutti C Jr,Sallum MA

更新日期：2007-09-01 00:00:00

abstract：:Surfacen® is an exogenous natural lung surfactant, composed by phospholipids and hydrophobic proteins, which is applied successfully in Newborn Respiratory Distress Syndrome. In this paper, in vitro activity of Surfacen® against Leishmania amazonensis is described. The product showed activity against the amastigote fo...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Blanco O,Lugones Y,Díaz E,Monzote L

更新日期：2011-07-01 00:00:00

abstract：:Fifty patients with epilepsy seen in three Londrina Neurological Services, in Paraná, were studied. The positivity prevalence of the enzyme-linked immunosorbent assay (ELISA) for cysticercosis in the cerebrospinal fluid (CSF) and in the serum of these patients was 34.0% and 20.0%, respectively. There was statistically...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Bonametti AM,Basile MA,Vaz AJ,Baldy JL,Takiguti CK

更新日期：1992-09-01 00:00:00

abstract：:This clinical case presents a patient with a raised and ulcerative lesion with erythematous edges in the mouth, on the lower lip that was unsuccessfully treated as herpes labialis. Clinical data and laboratory tests (Montenegro skin test, indirect immunofluorescence, direct parasite search and polymerase chain reactio...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Oyama J,Ferreira FBP,Conter CC,Lera-Nonose DSSL,Ramos-Milaré ÁCFH,Venazzi EAS,Silveira TGV,Lonardoni MVC

更新日期：2018-01-01 00:00:00

abstract：:The availability of an ultramicroanalytic system (SUMA) and species-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstr...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Laferte J,Abreu EG,Robaina R,Verez V

更新日期：1991-11-01 00:00:00

abstract：:Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, ...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Argañaraz ER,Hubbard GB,Ramos LA,Ford AL,Nitz N,Leland MM,Vandeberg JL,Teixeira AR

更新日期：2001-09-01 00:00:00

abstract：:Nosocomial infection among male patients in a public psychiatric hospital was studied and the definitions for use in long-term care facilities were employed for diagnosis. The overall nosocomial infection rate was 6.7 per 1,000 day inpatients; 55.6% of these infections were identified in the respiratory tract, 50% of ...

journal_title：Revista do Instituto de Medicina Tropical de Sao Paulo

authors： Almeida RC,Pedroso RP

更新日期：1999-11-01 00:00:00