Abstract：
:We investigated the pathogenesis of lung injury in sepsis (septic adult respiratory distress syndrome) by focusing on the functional changes of alveolar macrophages (AMs). Sepsis was induced in male WK rats by cecal ligation and puncture. Histological examination of the lungs from this experimental model revealed edematous change at 24 h after the surgery. The protein and endotoxin concentrations in the bronchoalveolar lavage fluid (BALF) increased with time after the surgery. The time course studies of AM function after surgery indicated that AMs from septic rats were activated by endotoxins. Specifically, this was suggested by the finding that AM adherence to and spreading on a plastic dish had increased. On stimulation, these AMs enhanced generation of superoxide anions and increased release of lysosomal enzymes, such as beta-glucuronidase. On the other hand, AMs in sepsis generated much smaller amounts of arachidonate lipoxygenase metabolites, such as leukotriene B4 (LTB4) and 12- and 5-hydroxyeicosatetraenoic acids (HETEs), on stimulation than did AMs from sham rats or untreated rats. However, the concentrations of immunoreactive LTC4 in the BALF of septic rats seemed to be higher than in untreated rats. It is suggested that the AMs of septic rats released lipoxygenase metabolites in alveoli and that these AMs could not be stimulated in vitro. These functional changes in the AMs of septic rats progressed along with the sepsis. These results implicate AMs in the development and progression of septic lung injury by releasing superoxide anions, beta-glucuronidase, and arachidonate metabolites. Furthermore, we speculate that reduced production of LTB4 by septic AMs may increase host susceptibility to severe pulmonary infection during septic ARDS.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Goya T,Abe M,Shimura H,Torisu Mdoi
10.1002/jlb.52.2.236subject
Has Abstractpub_date
1992-08-01 00:00:00pages
236-43issue
2eissn
0741-5400issn
1938-3673journal_volume
52pub_type
杂志文章abstract：:Besides their growth-inhibiting and differentiation-inducing properties, retinoids have been shown to exert immunomodulatory and anti-inflammatory functions by mechanisms that are not well understood. Tumor necrosis factor-alpha (TNF), a cytokine produced by mononuclear phagocytes, has been shown to be an important me...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.55.3.336
更新日期：1994-03-01 00:00:00
abstract：:DCs orchestrate immune responses contributing to the pattern of response developed. In cancer, DCs may play a dysfunctional role in the induction of CD4(+)CD25(+)Foxp3(+) Tregs, contributing to immune evasion. We show here that Mo-DCs from breast cancer patients show an altered phenotype and induce preferentially Treg...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0112048
更新日期：2012-09-01 00:00:00
abstract：:Murine reconstitution assays were used to investigate the effects of recombinant human interleukin-7 (rhIL-7) on myeloid and lymphoid precursors and on bone marrow engraftment. Reconstitution with bone marrow from rhIL-7-treated mice results in a 3.4-fold decrease in total colony-forming unit-spleen (CFU-S) activity (...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.58.2.151
更新日期：1995-08-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0213089
更新日期：2013-07-01 00:00:00
abstract：:Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well know...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.2A1013-564R
更新日期：2014-09-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 评论,杂志文章
doi：10.1189/jlb.0511241
更新日期：2012-07-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0109042
更新日期：2009-10-01 00:00:00
abstract：:The relationship between leukocyte migration and parenchymal cell death in vivo remains poorly documented. Accordingly, cell killing in the rat mesentery, as recorded by propidium iodide staining, was investigated with an intravital approach. Superfusion of platelet-activating factor (PAF, 10(-8) M) or N-formyl-methio...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.62.2.163
更新日期：1997-08-01 00:00:00
abstract：:An in vitro system allowing the culture of ovine bone marrow-derived macrophages (BMMs) is described. Bone marrow (BM) cells from the sternum of 4- to 9-month-old sheep were cultured in liquid suspension in hydrophobic bags with medium containing 20% autologous serum and 20% fetal calf serum (FCS). Cells with macropha...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.51.6.525
更新日期：1992-06-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
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更新日期：2012-12-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:We examined the relationship of I-A expression by normal murine macrophages to their immunoregulatory role on natural killer cell activity. Macrophages were isolated on the basis of plastic adherence; characterized on the basis of conventional markers such as phagocytic ability, cytoplasmic non-specific esterase activ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.48.5.382
更新日期：1990-11-01 00:00:00
abstract：:Lipopolysaccharide (LPS) is a potent natural adjuvant, commonly used to amplify Th1 responses. Here, we report that systemic immunization using LPS generates large numbers of specific Th17 cells in murine small intestinal lamina propria. The priming of these Th17 cells required IL-23p19 production by bone marrow-deriv...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0909631
更新日期：2010-07-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.62.4.493
更新日期：1997-10-01 00:00:00
abstract：:The in vitro effects of 17 alpha AED, the isomer of 5-androstene-3 beta,17 beta diol (17 beta AED) on the basal growth of murine RAW 264.7, P388D1, and human HL-60 cells were investigated. 17 alpha AED treatment of RAW cells for 48 h reduced total cell number without increasing cell death as detected by trypan blue ex...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.62.2.258
更新日期：1997-08-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Mycoplasmas and mycoplasma membranes have been shown to induce the production of inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, as well as nitric oxide, by mouse macrophages and rat brain astrocytes. Luminol-enhanced chemiluminescence was used as a sensitive method to show that Mycopl...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.57.2.264
更新日期：1995-02-01 00:00:00
abstract：:During the neoplastic progression, macrophages as well as dendritic and NK cells are attracted into the tumor site and initiate the immune response against transformed cells. They activate and present tumor antigens to T cells, which are then activated to kill tumor cells. However, tumor cells are often capable of esc...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.1105656
更新日期：2006-10-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.65.6.786
更新日期：1999-06-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.48.3.281
更新日期：1990-09-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a naturally occurring growth factor produced by several cell types in response to a variety of stimuli. GM-CSF has potent stimulatory effects on the growth and maturation of hematopoietic cells and has profound effects on mature circulating effector cells. C...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1002/jlb.58.6.634
更新日期：1995-12-01 00:00:00
abstract：:It has been shown that lead (Pb) potentiates lipopolysaccharide (LPS) lethality in animals by increasing the secretion and uptake or reactivity of tumor necrosis factor-alpha (TNF-alpha). Herein we report that PbCl2 increased TNF-alpha secretion from LPS-treated human peripheral blood mononuclear cells (PBMC) in a con...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.59.6.932
更新日期：1996-06-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.3A0216-082R
更新日期：2017-01-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0709513
更新日期：2010-04-01 00:00:00
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journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:Radiation-induced chimeric mice were used to study the origin of pulmonary alveolar macrophages. Unlike in other studies, these radiation chimeras were prepared by using a special fractionated irradiation regimen to minimize the killing of alveolar macrophage colony-forming cells, putative local stem cells. For this s...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:Postnatal stem cells regulate the homeostasis of the majority of our tissues. They continuously generate new progenitors and mature, functional cells to replace old cells, which cannot assume the tissue function anymore and are eliminated. Blood, skin, gut mucosa, muscle, cartilage, nerves, cornea, retina, liver, and ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.0505272
更新日期：2005-10-01 00:00:00
abstract：:Interleukin-1beta is a secreted protein that accumulates in the cytosol as an inactive precursor (pIL-1beta) before processing and release of biologically active protein. To understand the impact of this property on IL-1beta production, we examined the intracellular stability of pIL-1beta in lipopolysaccharide (LPS)-s...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
