Abstract：
:Cell surface aminopeptidases N (APN) and A (APA) have been characterized on cultured human glomerular epithelial cells and a SV40-transformed cell line derived from them. APN had a wide substrate specificity whereas APA only attacked peptides with an acidic N terminal amino acid. Both enzymes also differed by their sensitivity to divalent cations and to aminopeptidase inhibitors. Phorbolmyristate acetate (PMA) stimulated APN but not APA expression after a lag time of 12 hours. An increase of twice the basal value was observed with 10 ng.ml-1 PMA. This effect was confirmed by immunofluorescence staining using a specific anti-APN monoclonal antibody. Both ecto- and total enzyme activities were stimulated by PMA. The effect of PMA was suppressed by H7, a PKC inhibitor, and cycloheximide, an inhibitor of protein synthesis. Thrombin (1 to 2.5 U.ml-1) and interferon (IFN)-gamma (100 U.ml-1) also stimulated APN activity, the latter after longer exposure of the cells. APA activity was increased by 8-bromo-cAMP and two cAMP-stimulating agents, forskolin and isobutylmethylxanthine (IBMX). A twofold increase above basal value was obtained with 100 microM forskolin after 72 hours of treatment. cAMP-stimulated APA activity was suppressed by cycloheximide. Dexamethasone also stimulated APA activity. The effects of forskolin and dexamethasone were additive. These results demonstrate that APN and APA in glomerular epithelial cells are under different regulations: mitogens and IFN-gamma for APN, cAMP and glucocorticoids for APA. This selective expression may imply possible functional consequences in glomerular diseases.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Stefanovic V,Vlahovic P,Ardaillou N,Ronco P,Ardaillou Rdoi
10.1038/ki.1992.227subject
Has Abstractpub_date
1992-06-01 00:00:00pages
1571-80issue
6eissn
0085-2538issn
1523-1755pii
S0085-2538(15)57656-4journal_volume
41pub_type
杂志文章abstract：:Major temporal changes in the case mix of patients accepted for renal replacement therapy impede analysis of long-term mortality trends in end-stage renal disease patients. A record linkage study of 2 large electronic hospital inpatient data sets with national mortality data used standardization by age, sex, and comor...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2018.01.002
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Hemodialysis access thrombosis is a significant cause of morbidity for hemodialysis patients and results from decreased access flow caused by venous outflow tract stenosis. Ultrasound dilution (UD) is a practical, noninvasive, and reliable indicator of access flow and is effective in predicting venous stenos...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1046/j.1523-1755.2001.00753.x
更新日期：2001-06-01 00:00:00
abstract：:Polycystic renal disease was induced in rats by feeding 2-amino-4,5-diphenylthiazole. Tubular (TBM) and glomerular basement membranes (GBM) were purified and analyzed for possible structural changes that may be a factor in the development of the tubular dilations and cysts. Changes in the relative quantities of TBM po...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1985.193
更新日期：1985-11-01 00:00:00
abstract：:Hypoxia in the embryo is a frequent cause of intra-uterine growth retardation, low birth weight, and multiple organ defects. In the kidney, this can lead to low nephron endowment, predisposing to chronic kidney disease and arterial hypertension. A key component in cellular adaptation to hypoxia is the hypoxia-inducibl...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.06.015
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:To identify susceptibility genes for diabetic nephropathy, GeneChip Expression Analysis was employed to survey the gene expression profile of diabetic KK/Ta mouse kidneys. METHODS:Kidneys from three KK/Ta and two BALB/c mice at 20 weeks of age were dissected. Total RNA was extracted and labeled for hybridiz...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00312.x
更新日期：2003-12-01 00:00:00
abstract：:Severe segmental glomerulonephritis (Seg GN) (greater than or equal to 50% involvement) in systemic lupus erythematosus (SLE) is classified as diffuse GN (DPGN) in the WHO classification. We tested the validity of the assumption that severe Seg GN and DPGN have the same prognosis by determining the proportion of glome...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1987.203
更新日期：1987-08-01 00:00:00
abstract：:We compared growth rate, food conversion ratio and morphology of the growth zone in female Sprague-Dawley rats with subtotal nephrectomy or sham operation. Both groups were either given vehicle or 1.4 IU/day recombinant human growth hormone (GH) by s.c. osmotic minipump, or 2.5 IU twice daily intraperitoneally for 14 ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.7
更新日期：1988-01-01 00:00:00
abstract：:A study of dialysis costs in five major dialysis centers throughout the United States was conducted in 1973. When available, home, home training, limited care, satellite and in-hospital-full-care dialysis were included. The study's objective was to determine the cost per dialysis for each separate dialysis modality. A...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1976.31
更新日期：1976-03-01 00:00:00
abstract：:A newly described glomerulotubular feedback loop may explain the relationship between glomerular damage, epitope spreading, tubulointerstitial nephritis, proteinuria as a progression factor, and the importance of the local milieu in kidney damage. It also opens the horizons for exciting innovative approaches to therap...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2009.489
更新日期：2010-03-01 00:00:00
abstract：:Myeloproliferative neoplasms are clonal hematopoietic stem cell disorders that can produce an undefined glomerulopathy. To better characterize the glomerular disease associated with myeloproliferative neoplasms, we evaluated features of 11 patients with myeloproliferative neoplasm-related glomerulopathy that included ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.147
更新日期：2011-10-01 00:00:00
abstract：:Spleen tyrosine kinase (SYK) is an important component of the intracellular signaling pathway for various immunoreceptors. Inhibition of SYK has shown promise in preclinical models of autoimmune and glomerular disease. However, the description of SYK expression in human renal tissue, which would be desirable ahead of ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2015.29
更新日期：2015-07-01 00:00:00
abstract：:In the present study we assessed the prevalence of thin glomerular basement membrane (TGBM) in a large group of native kidney biopsies done for evaluation of renal disease. TGBM was present in 54 of 1078 biopsies (5%). In 12 of 54 biopsies (24%), TGBM was the only abnormality present. In the remaining biopsies TGBM wa...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.296
更新日期：1994-08-01 00:00:00
abstract：:Using a combination of intra-operative digital photography and micro-biopsy we measured renal cortical and papillary changes in five patients with primary hyperparathyroidism and abundant calcium phosphate kidney stones. Major tissue changes were variable papillary flattening and retraction, dilation of the ducts of B...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.161
更新日期：2008-07-01 00:00:00
abstract：:The erythropoietin analogs have been an important advance for the treatment of the anemia of kidney disease, resulting in reduced need for blood transfusion and improved quality of life. Recent studies, however, have indicated risks associated with targeting higher levels of hemoglobin (Hb). As a result, in March 2007...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5002401
更新日期：2007-10-01 00:00:00
abstract：BACKGROUND:Gene expression analysis is an invaluable tool in the study of diabetic glomerulosclerosis. The necessary denominator for the quantitative expression of a specific gene is the expression level of a second gene that is presumed to remain unchanged. Thus, it is critical that the stability of this housekeeping ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2004.66016.x
更新日期：2004-12-01 00:00:00
abstract：:We describe a rat model of renal failure that separates catabolic and anabolic states from each other. Muscle protein synthesis was compared during the anabolic period between sham (S) operated and renal failure (RF) rats that were fed different levels of dietary protein. Male rats weighing between 60 and 80 g first h...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.104
更新日期：1991-05-01 00:00:00
abstract：:Targeted therapies and immune checkpoint inhibitors have advanced the treatment landscape of Renal Cell Carcinoma (RCC) over the last decade. While checkpoint inhibitors have demonstrated survival benefit and are currently approved in the front-line and second-line settings, primary and secondary resistance is common....
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1016/j.kint.2020.08.028
更新日期：2021-01-01 00:00:00
abstract：:Previous studies have demonstrated that dietary potassium deprivation in healthy human subjects eating diets otherwise containing normal quantities of NaCl is accompanied by an increase in urinary calcium excretion. This increase in urinary Ca excretion occurs in association with reductions in urinary Na and Cl excret...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1995.134
更新日期：1995-03-01 00:00:00
abstract：:The adaptation of erythropoietin production to oxygen supply is determined by the abundance of hypoxia-inducible factor (HIF), a regulation that is induced by a prolyl hydroxylase. To identify cells that express HIF subunits (HIF-1alpha and HIF-2alpha) and erythropoietin, we treated Sprague-Dawley rats with the prolyl...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.460
更新日期：2010-02-01 00:00:00
abstract：:Fibrates, the PPAR alpha ligand-like compounds increase the expression of proximal tubule liver fatty acid binding protein (L-FABP) and significantly decrease cisplatin-induced acute kidney injury. To study whether the bezafibrate-mediated upregulation of renal L-FABP was involved in this cytoprotective effect we trea...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.106
更新日期：2008-06-01 00:00:00
abstract：:Early identification of kidney transplant recipients at risk of progressive allograft dysfunction may allow clinicians to provide closer monitoring and more aggressive risk factor modification. In this issue, Raynaud et al. presented a latent class model that clustered kidney transplant recipients into 8 risk categori...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2020.07.031
更新日期：2021-01-01 00:00:00
abstract：BACKGROUND:1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], the active form of vitamin D3, is a potent immunomodulatory agent on several cell types such as monocytes and mesangial cells. Recruitment of inflammatory cells, as well as stimulation of resident cells and mesangial matrix accumulation are key features of various expe...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00775.x
更新日期：2001-07-01 00:00:00
abstract：:The Diabetes Control and Complications Trial (DCCT) has demonstrated that intensive diabetes treatment delays the onset and slows the progression of retinopathy, nephropathy, and neuropathy in patients with IDDM. A detailed description of the effects of this treatment on diabetic nephropathy is presented here. In the ...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1038/ki.1995.236
更新日期：1995-06-01 00:00:00
abstract：BACKGROUND:The rat renal proximal tubule cells contain a large amount of monoamine oxidase, which catalyzes the oxidative deamination of catecholamines such as dopamine (DA). The aim of this study is to investigate the potential role of hydrogen peroxide (H2O2) produced by monoamine oxidase (MAO) isoform on regulation ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00468.x
更新日期：2001-01-01 00:00:00
abstract：:In studying the metabolic effects of diet potassium (K+) variation in normal humans, we noted that varying diet K+ within its normal range influenced inorganic phosphorus (Pi) homeostasis and serum calcitriol (1,25-dihydroxyvitamin D) levels. In six men who ingested a constant whole-foods diet containing (per 70 kg bo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1990.120
更新日期：1990-05-01 00:00:00
abstract：:The classic definition of hypercalciuria, an upper normal limit of 200 mg/day, is based on a constant diet restricted in calcium, sodium, and animal protein; however, random diet data challenge this. Here our retrospective study determined the validity of the classic definition of hypercalciuria by comparing data fro...
journal_title：Kidney international
pub_type： 杂志文章,meta分析,评审
doi：10.1038/ki.2011.227
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND:Current renal substitution therapy for acute or chronic renal failure with hemodialysis or hemofiltration is life sustaining, but continues to have unacceptably high morbidity and mortality rates. This therapy is not complete renal replacement therapy because it does not provide active transport nor metaboli...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00486.x
更新日期：1999-06-01 00:00:00
abstract：BACKGROUND:Tamm-Horsfall glycoprotein (THP) is a unique protein that is produced exclusively by cells of the thick ascending limb of Henle's loop (TALH). This study examined whether dietary salt altered renal THP production. METHODS:Male Sprague-Dawley rats were examined on days 1, 4, and 15 following placement in met...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00117.x
更新日期：1998-10-01 00:00:00
abstract：:Melamine, a widely used chemical found in many products in daily use, became a public health concern due to melamine-associated urinary stone formation in children. In adults, it is still unknown whether low-dose melamine exposure may also cause urolithiasis. To address this question, we studied 211 Taiwanese patients...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.154
更新日期：2011-10-01 00:00:00
abstract：:In early clinical testing, acute addition of alanyl-glutamine (AlaGln) to glucose-based peritoneal dialysis (PD) fluids restored peritoneal cellular stress responses and leukocyte function. This study was designed to test the effect of extended treatment with AlaGln-supplemented PD fluid on biomarkers of peritoneal he...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.kint.2018.08.031
更新日期：2018-12-01 00:00:00
