Abstract:
:When HeLa cells were incubated with tritiated dexamethasone mesylate at 2 degrees C, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cytosolic and nuclear extracts revealed the presence of two monomeric receptor complex forms with estimated molecular masses of about 98 and 87 kDa. If cells were subjected to crosslinking with glutaraldehyde, a third form consisting of a 250 kDa oligomer was also detected. When HeLa cells were treated with dexamethasone mesylate at 37 degrees C, and were subjected to crosslinking, electrophoresis of cytosolic glucocorticoid-receptor complexes was drastically reduced, whereas their levels in nuclear extracts were not appreciably altered.
journal_name
Life Scijournal_title
Life sciencesauthors
Rossini GP,Malaguti Cdoi
10.1016/0024-3205(92)90562-4subject
Has Abstractpub_date
1992-01-01 00:00:00pages
1517-25issue
19eissn
0024-3205issn
1879-0631pii
0024-3205(92)90562-4journal_volume
51pub_type
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