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Determination of immunoglobulin classes and subclasses of sperm immobilizing antibodies in infertile women and the inhibitory effect of IgG4 subclass on the sperm immobilizing antibody activity.

Abstract：

:Immunoglobulin classes and subclasses of sperm immobilizing antibodies (SI-Abs) in the sera of sterile women were determined by the absorption of patients' sera with Staphylococcus aureus and immunoadsorbents bound with class or subclass-specific anti-human immunoglobulin antibodies. Among 18 patients' sera tested, 16 had the IgG-dominant SI-Abs and the remaining 2 sera contained IgM-dominant SI-Abs. From the former patients' sera, 9 were further studied to determine the IgG subclasses; 6 of them had IgG1-dominant SI-Abs, one IgG2-dominant, and in the other two IgG1 and IgG2 were equally dominant. Interestingly, SI-Ab activities in 6 of the 9 patients' sera increased after absorption of IgG4 subclass and the addition of IgG4 purified from an SI-positive patient to the same patient's serum diminished the SI-Ab activities.

journal_name

J Reprod Immunol

journal_title

Journal of reproductive immunology

authors

Tsuji Y,Tatsumi T,Kameda K,Koyama K,Isojima S

doi

10.1016/0165-0378(93)90077-u

subject

Has Abstract

pub_date

1993-08-01 00:00:00

pages

223-33

issue

3

eissn

0165-0378

issn

1872-7603

pii

0165-0378(93)90077-U

journal_volume

24

pub_type

杂志文章

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