Abstract:
BACKGROUND/PURPOSE:Microgastria and postgastrectomy morbidities are substantial. The authors hypothesized a functional living tissue-engineered stomach could function as a replacement alternative. METHODS:Stomach organoid units, mesenchymal cores surrounded by epithelia, were isolated from neonatal and adult rats and transplanted paratopically on biodegradable polymer tubes, which were implanted in syngeneic hosts, varying the inclusion of stomach regions. Four weeks later, tissue-engineered stomach (TES) was either harvested or anastomosed. GFP labeling was performed before implantation. Histology and immunohistochemical detection of the antigensgastrin and actin smooth muscle were performed. RESULTS:Ninety-eight percent of all animals generated TES, including TES formation from adult tissue. Immunohistochemistry for alpha-actin smooth muscle and gastrin confirms the presence of a smooth muscle layer and a well-developed gastric epithelium containing all the elements of the native rat stomach including gastric pits and squamous layers, varying by included regions at harvest. TES architecture was maintained in anastomosis: GFP-labeled TES maintained signal in anastomosis, proving the donor origin of the TES. CONCLUSIONS:TES resembles native stomach and maintains robust histology in anastomosis, a new versatile model for the study of gastric physiology and possible therapy.
journal_name
J Pediatr Surgjournal_title
Journal of pediatric surgeryauthors
Grikscheit T,Srinivasan A,Vacanti JPdoi
10.1016/s0022-3468(03)00386-5subject
Has Abstractpub_date
2003-09-01 00:00:00pages
1305-9issue
9eissn
0022-3468issn
1531-5037pii
S0022346803003865journal_volume
38pub_type
杂志文章abstract:BACKGROUND/PURPOSE:Since 2000, a substantial increase in injuries related to nonmotorized scooters (kickboards) has been observed among children. The authors conducted a population-based prospective study to characterize these injuries in comparison with skateboard-related injuries during the same period. METHODS:All ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章,多中心研究
doi:10.1016/s0022-3468(03)00571-2
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND/PURPOSE:Pulmonary hypertension plays a significant role in the pathophysiology of congenital diaphragmatic hernia (CDH). Although there has been an intensive research effort directed at mediators that may cause pulmonary vasoconstriction, no single agent has been identified. The authors hypothesize that ther...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(98)90675-3
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND/PURPOSE:The aim of this study was to determine early prognostic indicators for the prediction of long-term survival rate and timing of the liver transplantation after the Kasai operation. METHODS:A retrospective chart study was performed on 142 patients with biliary atresia (BA). The patients were divided i...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1053/jpsu.2000.7765
更新日期:2000-07-01 00:00:00
abstract::The cumulative evidence supports nonoperative management of hepatic trauma in children who are hemodynamically stable and who require less than 50% blood volume replacement to maintain a stable blood count. This management approach, based on the use of either serial computed tomography scans, liver/spleen scans, or ul...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/0022-3468(92)90503-y
更新日期:1992-12-01 00:00:00
abstract::Over 200 cases of colonic hemangioma have been reported, with more than 50% occurring in the rectosigmoid. These benign vascular neoplasms usually present in childhood, often with life-threatening hemorrhage. Lack of awareness of the condition continues to lead to errors in diagnosis and management, and may contribute...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(88)80018-6
更新日期:1988-11-01 00:00:00
abstract::Postoperative pain control (PPC) in children is a difficult management problem. Systemic narcotics often result in respiratory depression, while nonnarcotic analgesics are associated with inconsistent PPC. This report reviews a 29-month (January 1989 through July 1991) experience with 174 children (aged < 18 years) wh...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/0022-3468(93)90616-s
更新日期:1993-04-01 00:00:00
abstract:BACKGROUND/PURPOSE:Studies in human surgical neonates have shown that intraoperative fentanyl analgesia results in greater fall in perioperative body core temperature compared with morphine analgesia. The aim of the study was to compare in a neonatal animal model the biochemical effect of fentanyl and morphine on hepat...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(99)90186-0
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVE:Reconstruction of the esophagus in children remains a challenge. Although jejunal grafts retain peristaltic activity, large series with long-term follow up are rare. We like to present our experience in a series of 24 children. METHODS:In the period 1988 through 2005, 24 children received an orthotopic jejun...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2006.10.009
更新日期:2007-02-01 00:00:00
abstract::One of the major remaining problems in the management of children with anterior chest wall deformities is the lack of a widely accepted objective method of evaluating the severity of the deformity. This deficiency has made it difficult to evaluate indications for operation or the results of operative repair. Moiré pho...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(81)80694-x
更新日期:1981-06-01 00:00:00
abstract::We describe 3 children with dilatation of the intrahepatic bile duct, who had anomalous junctions of the cystic duct, 2 high and 1 low, without pancreaticobiliary maljunction. They were all male, and underwent excision of the gallbladder and the extrahepatic bile duct followed by a Roux-en-Y hepaticojejunostomy as a d...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2006.08.025
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND:In female infants undergoing herniorrhaphy, there is a reported incidence of 15%-20% of prolapsed adnexal structures. Our primary aim is to confirm the incidence of adnexal tissue in hernia sacs at the time of repair and to further delineate the clinical characteristics of this population at a major pediatri...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2019.03.018
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND/PURPOSE:A randomised controlled trial evaluating the role of video-assisted thoracoscopic surgery (VATS) in childhood empyema reported a failure rate of 16.6%. Our aim is to determine the outcome of VATS in a large series of children managed by 3 paediatric surgeons experienced in endoscopic surgery. METHOD...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2008.10.083
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND:Firearm injuries are now the third leading cause of death in children. Understanding the circumstances surrounding pediatric firearm injuries will allow for targeted injury prevention efforts. We hypothesized that younger children are more likely to be victims of unintentional firearm injury. METHODS:A mult...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章,多中心研究
doi:10.1016/j.jpedsurg.2018.10.050
更新日期:2019-02-01 00:00:00
abstract::Since ulcerative colitis is primarily a mucosal disease, total colectomy with rectal mucosectomy and ileo-endomuscular pull-through (Soave) offers many theoretical advantages. The diseased colon is removed, the anal sphincteric mechanism is preserved, and permanent ileostomy is avoided, bladder and sexual function are...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(81)80346-6
更新日期:1981-04-01 00:00:00
abstract:BACKGROUND:Surgical techniques for fixation of the testis are varied and subject to ongoing debate. Non-sutured techniques may avoid the theoretical morbidities of sutured fixation of the testis yet are criticized for insufficient prophylaxis against future torsion. This study aims to compare outcomes between sutured (...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2019.08.018
更新日期:2019-12-01 00:00:00
abstract::We have investigated manometrically and endoscopically 60 children with extended pH metering-documented gastroesophageal reflux (GER) and a control group of 14 children of comparable ages. In an attempt to simplify the evaluation of esophageal peristalsis, we measured the frequency of propulsive waves (in waves/hour) ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(89)80213-1
更新日期:1989-10-01 00:00:00
abstract::A 1-year-old girl presented with fever, asthenia, and splenomegaly with hypersplenism. Abdominal ultrasound scan and magnetic resonance imaging showed multiple nodular cystic masses in an enlarged spleen. The histological examination of the resected spleen showed a novel type of vascular tumor called littoral cell ang...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(00)90225-2
更新日期:2000-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy of laparoscopic pneumovesical ureter reimplantation for congenital malformation involving the vesicoureteral junction in children. METHODS:From January 2005 to October 2010, 45 cases (comprising 61 ureters) were diagnosed as megaureter caused by vesicoureteral junction obstruction. A...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2012.09.020
更新日期:2012-12-01 00:00:00
abstract::Although infestation with Ascaris lumbricoides causes various intestinal complications, massive gastrointestinal bleeding is rare. The authors report on 2 infants who presented with massive hematemesis. In the first patient, a gastroduodenoscopy was performed. Roundworms were found adhering to an oozing duodenal ulcer...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(03)00584-0
更新日期:2003-11-01 00:00:00
abstract::Scarcity of small donors results in a high mortality rate for children on liver transplant waiting lists. To alleviate this problem, we have recently started to reduce the size of livers from older donors to use in children. In the last year, a total of 20 liver transplants were performed in 17 patients, including sev...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/0022-3468(90)90753-v
更新日期:1990-11-01 00:00:00
abstract:OBJECTIVE:This meta-analysis seeks to discern the optimal management strategy in neonatal testicular torsion (NTT). METHODS:Reviewed all English-language articles published between 2005 and 2015 in Medline/Pubmed that had a defined diagnosis of NTT within the first thirty days of life, and discussed specific surgical ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章,meta分析
doi:10.1016/j.jpedsurg.2018.07.006
更新日期:2019-04-01 00:00:00
abstract:PURPOSE:Besides surgical complications, a variety of adverse events may affect patients' comfort and outcome. The purpose of this prospective study was to identify the incidence and impact of all unexpected events in pediatric surgical patients. METHODS:All unexpected events that occurred in our department during the ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2020.12.004
更新日期:2020-12-13 00:00:00
abstract::Poland syndrome is characterized by hypoplastic unilateral chest wall structures. These chest wall deformities may be associated with upper extremity anomalies. The association of Poland syndrome with either intercostal liver herniation or a spinocerebral deformity has been described, but there is no report of both fi...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2011.10.005
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/PURPOSE:Transanal endorectal pull-through (TEPT) is the latest development in treatment of Hirschsprung's disease (HD). This prospective study was designed to evaluate the safety and efficacy of 1 stage TEPT technique in the management of patients with HD. METHODS:One hundred forty-nine children (116 boys a...
journal_title:Journal of pediatric surgery
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.jpedsurg.2003.11.038
更新日期:2004-03-01 00:00:00
abstract::The cases of two infants with prenatally diagnosed cystic meconium peritonitis, who underwent minimal surgical procedure (drainage) first and elective surgery later, are reported. Just after birth tube drains were inserted under local anesthesia with ultrasonographic guidance. They underwent laparotomy, at 16 days in ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract:BACKGROUND:The management of malrotation in patients with congenital abdominal wall defects has varied among surgeons. We were interested in investigating the risk of midgut volvulus in patients with gastroschisis and omphalocele to help determine if these patients may benefit from undergoing a Ladd procedure. METHODS...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章,多中心研究
doi:10.1016/j.jpedsurg.2016.10.014
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND/PURPOSE:Intrauterine growth retardation (IUGR) infants have impaired gastrointestinal function with resultant feeding difficulties and predisposition to necrotizing enterocolitis. Supplemented amniotic fluid swallowed by the developing fetus is a potential prenatal treatment for IUGR. Rabbits have naturally ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2004.02.008
更新日期:2004-06-01 00:00:00
abstract:PURPOSE:Intussusception is one of the common causes of intestinal obstruction in early childhood. Although a genetic predisposition has been suggested in some cases, its etiology is considered to be incidental, and it has not been traditionally regarded as having any genetic basis. Authors report on cases of so-called ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/j.jpedsurg.2007.04.012
更新日期:2007-09-01 00:00:00
abstract::Recently, interest has been expressed in the correction of diaphragmatic hernia in utero as a solution to the problem of high mortality from this condition. Before such a procedure can be adopted in the human, studies aimed at establishing the contribution such advanced techniques would make in reducing mortality are ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(84)80010-x
更新日期:1984-02-01 00:00:00
abstract:BACKGROUND:After surgical correction of their esophageal atresia and tracheoesophageal fistula (EA-TEF), many patients exhibit evidence of esophageal dysmotility. Controversy exists as to whether the esophageal motility disorders result from denervation caused by surgery or from an inherent abnormal innervation of the ...
journal_title:Journal of pediatric surgery
pub_type: 杂志文章
doi:10.1016/s0022-3468(97)90457-7
更新日期:1997-11-01 00:00:00