Abstract:
:We investigated the expression and production of the interleukin-1 receptor antagonist (IL-1ra) in three human glioblastoma cell lines (LN443, LN444, LN859). Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated the expression of IL-1ra mRNA transcripts in the three cell lines. These three cell lines also expressed mRNA for IL-1 alpha, IL-1 beta, as well as IL-1 receptor type I and type II, suggesting the presence of an IL-1 autocrine loop in these cell lines. Northern blot analysis demonstrated that the IL-1ra mRNA expression increased with IL-1 beta or tumor necrosis factor (TNF)-alpha but not with GM-CSF stimulation in both LN443 and LN444 cell lines. PMA stimulation increased the mRNA expression in LN444 but not in LN443 cells. Immunocytochemical staining showed IL-1ra immunoreactivity in these three cell lines. ELISA on culture supernatants demonstrated that the IL-1ra was secreted from the cell lines in agreement with the mRNA expression. RT-PCR with isoform-specific primers showed that both intracellular and secreted forms of IL-1ra were expressed by the three cell lines, with a predominance of the intracellular form. In vivo study with RT-PCR and Northern blot analysis demonstrated IL-1ra mRNA in six out of 12 human glioblastoma and two out of five anaplastic astrocytoma tissues, although the expression level was not high in some cases. Immunohistochemistry demonstrated the presence of IL-1ra within the cytoplasm of tumor cells in six out of 10 glioblastomas in vivo. These results suggest a potential role of IL-1ra in regulation of the IL-1 autocrine loop in glioblastomas.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Tada M,Diserens AC,Desbaillets I,Jaufeerally R,Hamou MF,de Tribolet Ndoi
10.1016/0165-5728(94)90045-0subject
Has Abstractpub_date
1994-03-01 00:00:00pages
187-94issue
2eissn
0165-5728issn
1872-8421pii
0165-5728(94)90045-0journal_volume
50pub_type
杂志文章abstract::Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-t...
journal_title:Journal of neuroimmunology
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更新日期:2011-05-01 00:00:00
abstract::Functional studies of cerebrospinal fluid T lymphocytes during acute viral infections of the nervous system are rare. Recently, we had the opportunity to investigate the requirements for interferon-gamma (IFN-gamma) production of human in vivo activated (primary) cytotoxic T lymphocytes (CTL) generated during acute vi...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(91)90106-h
更新日期:1991-09-01 00:00:00
abstract::Changes in mitogen-induced splenocyte proliferation and NK activity were evaluated after acute (1 h) and chronic (6 d) in vivo treatment of rats with the synthetic cannabinoid compound CP-55,940. At a dose of 0.4 mg/kg i.p. it significantly inhibited the splenocyte proliferative response to PHA and NK activity but hal...
journal_title:Journal of neuroimmunology
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更新日期:1997-12-01 00:00:00
abstract::Multiple sclerosis (MS) is a highly prevalent demyelinating disorder, presumed to be driven by an autoimmune response toward the central nervous system (CNS) components. All currently available treatments modulate the immune system globally and besides the reduction of disease activity, they may also impose considerab...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章,评审
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更新日期:2017-10-15 00:00:00
abstract::The role of autoantibody responses in pathogenesis or progression of multiple sclerosis (MS) remains controversial. This is partly because the methods that can distinctly identify pathogenic antibody reactivities targeting native membrane proteins from the reactivities that originate as an epiphenomenon in such diseas...
journal_title:Journal of neuroimmunology
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更新日期:2010-04-15 00:00:00
abstract::Astrocytes provide crucial support for neurons and their impairment by viruses or their interactions with anti-viral or autoimmune responses could contribute to neurological disease. We have developed a transgenic mouse model to assess lymphocyte-astrocyte interactions. The major histocompatibility complex (MHC) class...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(94)90163-5
更新日期:1994-06-01 00:00:00
abstract::Immunological responses to protect against excessive inflammation can be regulated by the central nervous system through the cholinergic anti-inflammatory pathway wherein acetylcholine released from vagus nerves can inhibit inflammatory cytokines. Although a role for the α7 nicotinic acetylcholine receptor (α7 nAChR) ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2011.08.007
更新日期:2011-10-28 00:00:00
abstract::To study the role of cell adhesion molecules in the fatal CD8+ T-cell mediated meningitis which is induced by intracerebral infection with lymphocytic choriomeningitis virus, the expression of relevant molecules on inflammatory cells and local endothelium was analyzed immunohistochemically. Most inflammatory cells wer...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(95)00099-n
更新日期:1995-10-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:1984-11-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:2013-06-15 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2004.12.015
更新日期:2005-04-01 00:00:00
abstract::Serum levels of immunoreactive interleukin-2 (IL-2) were determined at monthly intervals from a group of placebo- and drug-treated chronic progressive multiple sclerosis patients before and during a cyclosporine A therapeutic trial. Significantly elevated levels of the lymphokine in active patients confirmed earlier s...
journal_title:Journal of neuroimmunology
pub_type: 临床试验,杂志文章
doi:10.1016/0165-5728(90)90036-m
更新日期:1990-06-01 00:00:00
abstract::Human Leukocyte Antigen G (HLA-G) gene polymorphism and expression rate have recently been suggested to have a potential role in susceptibility to Multiple Sclerosis (MS), a chronic inflammatory demyelinating and neurodegenerative disease of the central nervous system with unknown etiology. The aim of this study was t...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:2016-01-15 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2010.04.006
更新日期:2010-06-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.08.015
更新日期:2013-11-15 00:00:00
abstract::We evaluated the brain volume and rate of atrophy in 85 Japanese patients with multiple sclerosis (MS). The mean brain volume was smaller and the rate of atrophy appeared to be more rapid in MS patients than in normal subjects. The brain atrophy seemed to exist from the early stage of MS and was prominent in the grey ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2017.03.009
更新日期:2017-05-15 00:00:00
abstract::Three monoclonal antibodies reactive with human myelin basic protein have been used to develop immunoradiometric assays for this protein. Clone 1, a mouse IgG2a, is reactive with an epitope in the region 129-138. Clone 2, a mouse IgG1, is reactive with the region 119-131. Clone 12, a rat IgG, is reactive with the regi...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(86)90032-9
更新日期:1986-10-01 00:00:00
abstract::To assess whether TNF-alpha causes inflammatory demyelination or axonal degeneration, we injected into rat sciatic nerve saline, 100 U and 1000 U of rhTNF-alpha and studied the electrophysiological and pathological effects. At day 1 electrophysiology showed a slight reduction of proximal compound muscle action potenti...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(98)00229-x
更新日期:1999-02-01 00:00:00
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journal_title:Journal of neuroimmunology
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2018-09-15 00:00:00
abstract::Depression and chronic fatigue are frequently present in multiple sclerosis (MS); however, the prevalence rates have not been investigated in neuromyelitis optica (NMO). Thirty-nine consecutive NMO and 75 MS patients were compared using self-rating questionnaires for depressive states, daily activity, and fatigue, as ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:2015-06-15 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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abstract::Pathogenic autoreactive T cells can be targeted by T cell vaccination (TCV), a procedure in which patients are immunized with autologous attenuated pathogenic T cells. We reported previously that TCV with myelin basic protein (MBP) reactive T cell clones in multiple sclerosis (MS) patients induced T cell immune respon...
journal_title:Journal of neuroimmunology
pub_type: 临床试验,杂志文章
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更新日期:2000-01-03 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(97)00163-x
更新日期:1998-01-01 00:00:00
abstract::Emerging autoimmunity (epitope-spreading) generated as a consequence of myelin damage is suggested to underlie the relapses in multiple sclerosis (MS). Myelin oligodendrocyte glycoprotein (MOG 8-21) induces relapsing EAE in ABH mice characterized by broadening of the autoimmune reportoire. Despite epitope spreading to...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
abstract::In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(89)90092-1
更新日期:1989-09-01 00:00:00
abstract::For the epigenetic characterization of myasthenia gravis (MG), we determined whether long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) are expressed differentially in subjects with and without MG. Compared with healthy control subjects, the MG patients had 1561 upregulated lncRNAs, 1034 downregulated lncRNAs, 9...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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