Abstract:
:The hypolipidemic drug ethyl chlorophenoxyisobutyrate (clofibrate) is known to induce peroxisome proliferation and to be carcinogenic after long term administration to rats and mice. We examined the effects of treatment with this drug for periods of up to 18 months on cytosolic ATP-stimulated and ADP-inhibited acetyl-CoA hydrolase in rat liver. In male Donryu albino rats on a diet containing 0.5% clofibrate, the enzyme activity increased to about 2- and 3-fold the initial level per milligram liver protein and cytosolic protein, respectively, and 2-fold per milligram DNA in 3 days, and then remained at this level for up to 18 months. The increased activity in rats receiving clofibrate for 3 months returned to control level within a week when clofibrate was withdrawn. The change in enzyme activity paralleled the change in the amount of enzyme protein determined by immunoblotting with antibody against purified acetyl-CoA hydrolase from rat liver cytosol. No liver tumors were detected macroscopically after administration of clofibrate for 18 months. However, our results suggest that cytosolic acetyl-CoA hydrolase could be an extraperoxisomal marker enzyme induced by this type of drug.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Nakanishi Y,Okamoto K,Isohashi Fdoi
10.1016/0006-2952(93)90038-xsubject
Has Abstractpub_date
1993-04-06 00:00:00pages
1403-7issue
7eissn
0006-2952issn
1873-2968pii
0006-2952(93)90038-Xjournal_volume
45pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/0006-2952(93)90002-e
更新日期:1993-05-25 00:00:00
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pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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更新日期:1991-04-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(85)90189-3
更新日期:1985-02-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章,评审
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更新日期:2018-09-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/j.bcp.2016.11.001
更新日期:2017-02-01 00:00:00