Abstract:
BACKGROUND:Accurate typing of a patient's RBCs in the setting of prior transfusion or a hemolytic transfusion reaction is crucial in the selection of compatible blood but is time consuming, technically difficult, and sometimes impossible. To address this problem, a simple, rapid, and inexpensive quantitative PCR method was developed to identify the single nucleotide polymorphism (SNP) of the Kidd blood group. We applied this method in a clinical investigation of 54 multiple-transfusion patients. STUDY DESIGN AND METHODS:Patients were eligible if they had received at least one RBC transfusion within 30 days and had a sample referred to our regional reference lab for assistance with compatibility testing requiring reticulocyte separation, hypotonic saline treatment, or chemical modification to remove IgG. We compared serologic result to the normalized quantitative PCR. For discrepants, or where no serologic type could be assigned, DNA sequencing characterized the patient's Kidd SNP. RESULTS:Of the 54 patients, the reference lab could assign a serologic Kidd type for 33. Quantitative PCR assigned a Kidd type for 53 of the 54. In three cases, where serology and PCR were discrepant, and for all cases where serology could not assign a Kidd type, DNA sequencing verified the Kidd typing assigned by PCR. CONCLUSION:A simple, rapid, and accurate technique has been developed. The assay performs well in the clinical setting. With further study, and inclusion of other blood group systems, this may become an important supplemental technique for selected patients in the immunohematology reference laboratory.
journal_name
Transfusionjournal_title
Transfusionauthors
Montalvo L,Walker P,Wen L,Lim W,Reed W,Busch MP,Lee THdoi
10.1111/j.1537-2995.2004.03303.xsubject
Has Abstractpub_date
2004-05-01 00:00:00pages
694-702issue
5eissn
0041-1132issn
1537-2995pii
TRF03303journal_volume
44pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Flow cytometric measurement of CD34+ events is used to ensure the quality of human progenitor cell grafts. This study was conducted to evaluate whether the spiking of routine samples from peripheral blood and apheresis products with CD34+ positive controls is feasible. STUDY DESIGN AND METHODS:A total of 42...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03259.x
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Uncommonly, antibodies that appear to exhibit antigenic specificity on red blood cell (RBC) panels fail to maintain specificity following alloadsorption (i.e., they mimic antigenic specificity). Understanding both the pitfalls and the proper pathways to establish the diagnosis and to interpret the clinical s...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.0041-1132.2004.04112.x
更新日期:2004-09-01 00:00:00
abstract::In Taiwan, the prevalence of circulating anti-HCV is 2 percent among first-time voluntary Chinese blood donors, 10 percent among donors with elevated ALT levels (greater than 45 IU/L), and higher among older men. The carrier rate for HBsAg was 18.6 percent and the frequency of positive HBV marker(s) (HBsAg, anti-HBc, ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1990.30590296383.x
更新日期:1990-06-01 00:00:00
abstract::This study evaluated various algorithms for the generation of a red cell antibody identification panel by computer. The algorithms depended on schemes that optimized Fisher's exact p value for each specificity in the panel rather than using predetermined formulations of desired phenotypic combinations. Panels produced...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1987.27187121480.x
更新日期:1987-01-01 00:00:00
abstract::Three cases are presented in which the presence of sodium caprylate dependent albumin auto-agglutinins in the patients' sera resulted in erroneous Rh-hr phenotyping of their erythrocytes. In the initial testing, the albumin control was negative in each case. Since one of the cases was an Rh0(D) negative individual who...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1975.15576082226.x
更新日期:1975-09-01 00:00:00
abstract:BACKGROUND:The anti-CD20 monoclonal antibody rituximab has immune-modulatory effects similar to intravenous immunoglobulin (IVIG). We performed a systematic review and meta-analysis to determine the efficacy and safety of rituximab in autoimmune diseases that are also treated with IVIG. STUDY DESIGN AND METHODS:The mo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14841
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Intravenous immunoglobulin G (IVIG) replacement therapy is used to prevent invasive infections in patients with primary antibody deficiency (PAD). However, few studies have functionally evaluated specific antibodies against encapsulated bacteria that cause invasive infection in patients with PAD. In this stu...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13869
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Hemolysis due to D incompatibility in the setting of liver transplantation is less frequent than that associated with ABO incompatibility, but can represent an equally adverse event. Approximately 10 percent of ABO-compatible liver transplants involve a D- donor and a D+ recipient. CASE REPORT:A case of sev...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.03382.x
更新日期:2004-11-01 00:00:00
abstract::A fluorometric assay is described for the detection of red blood cell antibodies. The assay reveals as little as 600 molecules of bound, fluoresceinated rabbit anti-human IgG antibodies per erythrocyte. Eleven patients with possible autoimmune erythrocyte disorder and negative direct antiglobulin test were studied by ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21281178153.x
更新日期:1981-03-01 00:00:00
abstract:BACKGROUND:Virus inactivation of plasma can be achieved by photodynamic methods in the presence of phenothiazine dyes such as methylene blue (MB). Subsequent filtration may increase the efficacy of virus inactivation and reduce adverse effects of WBC contamination and MB. STUDY DESIGN AND METHODS:This study examined t...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41010082.x
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Deferasirox (DFRA) is a new approved oral iron chelator. Its advantages are that it is convenient and better tolerated and adhered to due to "once-daily" oral dosage. However, its use in the field is limited and it is yet to be subjected to postmarketing surveillance. CASE REPORT:A 18.75-year-old male with ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02939.x
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND:Selection of CD34+ PBPCs has been applied as a method of reducing graft contamination from neoplastic cells. This procedure seems to delay lymphocyte recovery, while myeloid engraftment is no different from that with unselected PBPC transplants. STUDY DESIGN AND METHODS:Lymphocyte recovery was studied in tw...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41060783.x
更新日期:2001-06-01 00:00:00
abstract:BACKGROUND:The effect on component wastage after enhancing the clinician's ability to order blood products in the computerized physician order entry (CPOE) system was investigated in a multihospital network. METHODS:A novel field was added to the CPOE of eight hospitals within a health care system allowing the physici...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13399
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Immunomodulatory effects of UV light have increasingly become a focus in transfusion medicine, BMT and transplantation immunology. In the transplant setting, the use of UVB radiation may reduce or abolish T-cell activation without compromising either bone marrow (BM) engraftment or graft-versus-leukemia effe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40030361.x
更新日期:2000-03-01 00:00:00
abstract:BACKGROUND:During the COVID-19 outbreak, most hospitals deferred elective surgical procedures to allow space for the overwhelming number of COVID-19 patient admissions, expecting a decrease in routine blood component requirements. However, because transfusion support needs of COVID-19 patients are not well known, its i...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16171
更新日期:2020-11-04 00:00:00
abstract::This article introduces a new method of component preparation that is capable of producing white cell (WBC)-reduced platelet concentrates (PCs) from whole blood. Whole blood is separated into packed red cells (RBCs) and platelet-rich plasma (PRP) by centrifugation, and the PRP is expressed through a newly designed WBC...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31691306254.x
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:Immunoglobulin (Ig)G replacement with intravenous or subcutaneous immunoglobulins is a lifelong substitutive therapy in patients with primary antibody deficiencies (PADs). Hemolysis after immunoglobulin therapy was described in patients receiving high immunoglobulin dosages. The issue of hemolysis after immu...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12939
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:The potential hemostatic effect of infusible platelet membranes (IPM; Cyplex, Cypress Bioscience) prepared from outdated human platelets is investigated. STUDY DESIGN AND METHODS:Increasing concentrations of IPM were added to blood samples anticoagulated with low-molecular-weight heparin, in which platelets...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40091074.x
更新日期:2000-09-01 00:00:00
abstract:BACKGROUND:Beriplex P/N/Kcentra/Coaplex/Confidex is a four-factor human prothrombin complex concentrate (PCC). Here, we describe the pathogen safety profile and biochemical characteristics of an improved manufacturing process that further enhances the virus safety of Beriplex P/N. STUDY DESIGN AND METHODS:Samples of p...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14010
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Previous studies of bedside transfusion compatibility tests have shown high rates of erroneous transfusion decision, due to defective techniques and poor user performance. An experimental study was conducted to evaluate the error rate obtained with a new ready-to-use device (Vu-Test, Medigis), in comparison ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00219.x
更新日期:2002-10-01 00:00:00
abstract::A simple method for preparing relatively pure C3c fragment from serum-reacted zymosan is described. Although hyperimmunization studies indicate contamination of the "C3c" product by C3d and immunoglobulin antigenic materials, these are present in such small amounts that they do not impair the effectiveness of the prod...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1980.20380214904.x
更新日期:1980-05-01 00:00:00
abstract:BACKGROUND:Maternal iron deficiency anemia (IDA) impacts placenta and fetus. We evaluated effects of IDA at admission for delivery on cesarean rates, and adverse maternal and neonatal outcomes. STUDY DESIGN AND METHODS:Medical records from Jerusalem (2005-2012) identified women with a live-birth singleton fetus in cep...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13252
更新日期:2015-12-01 00:00:00
abstract::Previous studies have shown that some examples of anti-M and anti-N fail to agglutinate neuraminidase-treated (NeuNAc-depleted) red cells. This investigation extends those observations and shows that the antibodies fail to bind to such treated red cells. These observations may mean that NeuNAc residues act to orient t...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23283172846.x
更新日期:1983-03-01 00:00:00
abstract:BACKGROUND:This study was designed to evaluate the potential yield of introducing nucleic acid amplification testing (NAT) in blood donation, according to the detection threshold and the pool size. STUDY DESIGN AND METHODS:A mathematical model of early HIV-1 population dynamics in blood has been developed and is used ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1999.39101141.x
更新日期:1999-10-01 00:00:00
abstract:BACKGROUND:Thrombotic microangiopathies (TMA) are systemic vasoocclusive disorders associated with significant morbidity and mortality. Rapid and reliable diagnosis of TMA is critical. The diagnosis is complicated by a lack of objective and sensitive laboratory testing as well as multiple concurrent diseases, including...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00873.x
更新日期:2006-07-01 00:00:00
abstract::The authors describe a prototype elution method employing chloroquine, a quinoline derivative, to elute IgG antibodies from the platelet surface. This chloroquine elution technique is relatively easy to perform and is effective in the removal of alloantibodies from the platelet surface. Eluted alloantibody was immunol...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28288179016.x
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND:Chagas disease is caused by Trypanosoma cruzi, a protozoan parasite that can be transmitted by transfusion. The diagnosis of chronic T. cruzi infection is generally made by detecting specific antibodies that bind to parasite antigens. The aim of this study was to assess the sensitivity and specificity of a n...
journal_title:Transfusion
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1537-2995.2006.00965.x
更新日期:2006-10-01 00:00:00
abstract::This paper presents recent data from quantitative and thermodynamic investigations on A and B erythrocyte antigens. In regard to weak A phenotypes on the basis of quantitative data, practically no gap is observed from the weakest Aend to the strongest A3. Weak B phenotypes give a similar distribution. However, a more ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1976.16677060242.x
更新日期:1976-11-01 00:00:00
abstract:BACKGROUND:The use of hematopoietic progenitor cell (HPC) transplantation has rapidly expanded in recent years. Currently, several sources of HPCs are available for transplantation including peripheral blood HPCs (PBPCs), cord blood cells, and marrow cells. Of these, PBPC collection has become the major source of HPCs....
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12238
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Recent studies conducted in developed countries described hepatitis E virus (HEV) as an emerging infectious threat to blood safety. However, data on HEV among blood donors from southeast Asia are lacking. STUDY DESIGN AND METHODS:Between July and August 2014, we assessed the presence of HEV immunoglobulin (...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13731
更新日期:2016-10-01 00:00:00