Abstract:
:Recently, we demonstrated that approximately 60% of GLUT 1 in a crude membrane fraction of rat skeletal muscle originates from perineurial sheaths. To study the in vivo regulation of GLUT 1 expression in different tissues in muscles, we measured the level of GLUT 1 in crude muscle membranes and in perineurial sheaths in diabetic (fa/fa) Zucker rats and lean controls, with and without metformin treatment. The GLUT 1 concentration in perineurial sheaths was identical in all four groups of rats, both when measured by quantitative immunofluorescence and by immunoblotting and densitometry. In a fraction of crude membranes of soleus muscles GLUT 1 expression was more than two-fold higher in (fa/fa) rats than in lean controls (p < 0.005). Metformin treatment significantly elevated GLUT 1 in control rats (p < 0.05) and tended to decrease GLUT 1 in diabetic rats (p < 0.075). The expressions of GLUT 1 and GLUT 4 in crude muscle membranes were inversely correlated (p < 0.01), and GLUT 1 expression correlated positively with fasting glucose (p < 0.05). In conclusion, GLUT 1 expression in perineurial sheaths is unaffected by alterations in glucose homeostasis and by the genes responsible for obesity and diabetes in the Zucker rat. GLUT 1 expression in a crude membrane fraction of soleus muscle is increased in the diabetic animals, likely due to an increased expression in muscle cells proper.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Handberg A,Kayser L,Høyer PE,Micheelsen J,Vinten Jdoi
10.1007/s001250050130subject
Has Abstractpub_date
1994-05-01 00:00:00pages
443-8issue
5eissn
0012-186Xissn
1432-0428journal_volume
37pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract:AIMS/HYPOTHESIS:Coronary heart disease is the leading cause of mortality among people with type 1 diabetes. Diet is an important lifestyle factor that relates to risk of CHD. The aim of this study was to examine how diet and adherence to dietary guidelines differ between adults with and without type 1 diabetes, and the...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-009-1280-4
更新日期:2009-05-01 00:00:00
abstract::The capacity of reovirus type 3 to infect insulin-producing B cells was studied in human pancreatic cell cultures. Antibody to reovirus was labelled with fluorescein isothiocyanate and antibody to insulin was labelled with tetramethyl rhodamine isothiocyanate. By using a double-labelled immunofluorescent antibody tech...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00253408
更新日期:1981-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Gestational diabetes mellitus (GDM) identifies a population of young women at high risk of developing type 2 diabetes and thus provides an excellent model for studying early events in the natural history of this disease. Adiponectin, a novel adipocyte-derived protein with insulin-sensitising properties,...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1710-x
更新日期:2005-05-01 00:00:00
abstract::An autoimmune basis for the pathogenesis of insulin-dependent diabetes mellitus (IDDM) is supported by the frequent presence of autoantibodies - islet cell antibodies (ICAs) and GAD antibodies (GADab). However, in Chinese patients with clinical IDDM, a low prevalence of ICAs was observed. In non-insulin-dependent diab...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050845
更新日期:1997-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Low phosphate and high calcium concentrations have been linked to altered glucose tolerance and reduced insulin sensitivity in non-diabetic individuals. The aim of this study was to examine the relationships of calcium and phosphate levels and the calcium-phosphate product with the development of type 2...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-014-3241-9
更新日期:2014-07-01 00:00:00
abstract::There is ample evidence that environmental factors are involved in the aetiology of type 1 diabetes, but the nature and timing of the interactions are poorly understood. The intrauterine environment is known to play a role in the later development of type 2 diabetes, and this review considers a possible role in type 1...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s00125-013-2929-6
更新日期:2013-09-01 00:00:00
abstract::The impact of improved glycaemic control on renal function in newly-presenting Type 2 (non-insulin-dependent) diabetic patients has not been adequately researched. Consequently, glomerular filtration rate and effective renal plasma flow and urinary albumin excretion rates were determined in 76 subjects (age (mean (SD)...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00401144
更新日期:1993-08-01 00:00:00
abstract::We investigated whether the glomerular synthesis of prostaglandins modulates the glomerular filtration rate and albuminuria in diabetic nephropathy. The urinary excretion of immunoreactive prostaglandin E2 (253 pg/min) was significantly elevated in eight Type 1 (insulin-dependent) diabetic women with nephropathy as co...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF00274575
更新日期:1987-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Mitochondrial oxidative phosphorylation (OXPHOS) plays an important role in the pathophysiology of type 2 diabetes. Genes involved in OXPHOS have been reported to be down-regulated in skeletal muscle from patients with type 2 diabetes; however, hepatic regulation is unknown. MATERIALS AND METHODS:We an...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0489-8
更新日期:2007-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Patients with glucokinase mutations are characterised by mild, persistent fasting hyperglycaemia, a small increment in glucose in response to an oral load and a dominant family history. These patients frequently present with gestational diabetes and often require insulin treatment during pregnancy. We a...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050038
更新日期:2000-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Cardiovascular events and death are better predicted by postprandial glucose (PPG) than by fasting blood glucose or HbA(1c). While chronic exercise reduces HbA(1c) in patients with type 2 diabetes, short-term exercise improves measures of insulin sensitivity but does not consistently alter responses to ...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/s00125-012-2490-8
更新日期:2012-05-01 00:00:00
abstract::The involvement of the CNS in pancreatic hormone release has been studied. 1.) It has been shown that one source of vagal efferent fibers capable of facilitating insulin secretion originated in the rostral half of the nucleus ambiguus. 2.) Acute lesions of the ventromedial hypothalamus resulted in hyperinsulinaemia th...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00254511
更新日期:1981-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:Aggregation of islet amyloid polypeptide (IAPP) to form amyloid contributes to beta cell dysfunction in type 2 diabetes. Human but not non-amyloidogenic rodent IAPP induces islet macrophage proIL-1β synthesis. We evaluated the effect of IL-1 receptor antagonist (IL-1Ra) on islet inflammation and dysfunc...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-014-3447-x
更新日期:2015-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:Although maternal hyperglycaemia is associated with increased risk of adverse pregnancy outcome, the mechanisms of postprandial hyperglycaemia during pregnancy are poorly understood. We aimed to describe glucose turnover in pregnant women with type 1 diabetes, according to stage of gestation (early vs l...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/s00125-011-2363-6
更新日期:2012-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:We hypothesised that type 1 diabetic patients with established diabetic sensorimotor polyneuropathy (DSPN) would have segmental and/or pan-enteric dysmotility in comparison to healthy age-matched controls. We aimed to investigate the co-relationships between gastrointestinal function, degree of DSPN and...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-016-4199-6
更新日期:2017-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:A high prevalence of diabetes contributes to excess CHD in Indian Asians, but the underlying mechanisms are unclear. Heart rate, heart rate variability (HRV) and baroreflex sensitivity (BRS) are measures of cardiac autonomic function that are disturbed by hyperglycaemia and predict CHD. We compared thes...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-010-1818-5
更新日期:2010-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:The pathogenesis of vascular complications in type 1 diabetes is poorly understood, but may involve chronic, low-grade inflammation. We investigated the association of markers of inflammation with vascular complications in type 1 diabetes. METHODS:A cross-sectional nested case-control study of the foll...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究
doi:10.1007/s00125-004-1628-8
更新日期:2005-02-01 00:00:00
abstract::Mild hypoglycaemia was induced using an artificial pancreas in five normal subjects (from 5.00 +/- 0.15 to 2.83 +/- 0.15 mmol/l) by infusing 28 mU/m2 per min soluble insulin for 60 min. Six Type 1 (insulin-dependent) diabetic patients were stabilized for 14h using an artificial pancreas. They were then rendered hypogl...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00254837
更新日期:1982-02-01 00:00:00
abstract::The development of diabetic glomerulopathy in kidneys transplanted to diabetic patients was estimated in transplant biopsies and evaluated in relation to suspected clinical risk factors for diabetic nephropathy. Surgical biopsies were taken at baseline and at 24-36 months post-transplantation in 16 Type 1 (insulin-dep...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400997
更新日期:1991-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Preferential visceral adipose tissue (VAT) accumulation has been clearly associated with insulin resistance. In contrast, the impact of visceral obesity on beta cell function is controversial. METHODS:In 62 non-diabetic women and men (age 24-69 years, BMI 21-39 kg/m2), we measured VAT and subcutaneous ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1891-3
更新日期:2005-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this work was to examine the associations of average weight and weight velocity in three growth periods from birth through adolescence with type 2 diabetes incidence. METHODS:Child participants were selected from a 43 year longitudinal study of American Indians to represent three growth peri...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-020-05165-w
更新日期:2020-09-01 00:00:00
abstract::The authors have been made aware that the following sentence is incorrect: 'Like IIK7, both ramelteon and tasimelteon have a greater affinity for the MT2 receptor [162].' ...
journal_title:Diabetologia
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00125-018-4577-3
更新日期:2018-05-01 00:00:00
abstract:AIMS/HYPOTHESIS:According to the consensus criteria developed for type 1 diabetes, an individual can be diagnosed with insulitis when ≥ 15 CD45+ cells are found within the parenchyma or in the islet-exocrine interface in ≥ 3 islets. The aim of this study was to determine the frequency of individuals with type 2 diabete...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-016-4140-z
更新日期:2017-02-01 00:00:00
abstract::Previous study have reported a significant improvement of peripheral neuropathy following combined pancreas and kidney transplantation attributed to improvement of blood glucose control by some authors and to elimination of uraemia by others. To asses the specific role of uraemia and hyperglycaemia in neuropathy, 16 d...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00587632
更新日期:1991-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:We sought to elucidate whether excess glucocorticoids and increased dietary lipids act synergistically to impair glucose tolerance and, if so, whether activation of peroxisome proliferator-activated receptor alpha (PPARalpha) has an adverse or beneficial effect on glucose tolerance. METHODS:Dexamethaso...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1894-0
更新日期:2005-10-01 00:00:00
abstract::One of the reasons for the variability of blood glucose regulation in Type 1 (insulin-dependent) diabetic patients is the huge variation in subcutaneous absorption of intermediate-acting insulin. We have investigated the variation in insulin absorption during continuous subcutaneous insulin infusion in eight such pati...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00251817
更新日期:1983-05-01 00:00:00
abstract::Insulin antibodies expressed as insulin binding capacity of IgG were determined in 50 Type 1 (insulin-dependent) diabetic children who have been treated with monocomponent porcine insulin from the onset of the disease. During the follow-up period of 0.5-5.5 years (mean +/- SD: 3.2 +/- 1.6 years), 26 out of 50 patients...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00281121
更新日期:1984-02-01 00:00:00
abstract::To investigate the mechanism whereby changes in plasma glucose level alter human pancreatic A-cell activity in vivo, A-cell activity was determined during manipulation of plasma glucose and pancreatic B-cell activity by insulin and glucose infusions. A-cell activity (the acute immunoreactive glucagon response to intra...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00252408
更新日期:1984-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:Diabetic retinopathy is a serious complication of diabetes mellitus and can lead to blindness. A genetic component, in addition to traditional risk factors, has been well described although strong genetic factors have not yet been identified. Here, we aimed to identify novel genetic risk factors for sig...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3697-2
更新日期:2015-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:It has been proposed that muscle fibre type composition and perfusion are key determinants of insulin-stimulated muscle glucose uptake, and alterations in muscle fibre type composition and perfusion contribute to muscle, and consequently whole-body, insulin resistance in people with obesity. The goal of...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-021-05383-w
更新日期:2021-01-29 00:00:00