Detection of Chlamydia trachomatis in fallopian tube tissue in women with postinfectious tubal infertility.

Abstract:

OBJECTIVE:Biopsy tissues from women with postinfectious tubal infertility were studied for the presence of Chlamydia trachomatis. STUDY DESIGN:Tubal biopsy specimens from 25 women with postinfectious tubal infertility undergoing laparoscopy for repair of fallopian tubes were evaluated by culture, in situ hybridization. Immunocytochemistry, and transmission electron microscopy for the presence of Chlamydia trachomatis. Serum was also tested for Chlamydia trachomatis antibodies. RESULTS:Chlamydia trachomatis was detected in postinfectious tubal biopsy specimens in three of 25 patients by culture, 12 of 24 by in situ hybridization, 15 of 22 by immunoperoxidase stain, and two of 10 by transmission electron microscopy. Serum antibody against Chlamydia trachomatis was detected in 15 of 21 patients. CONCLUSION:Chlamydia trachomatis deoxyribonucleic acid or antigens were detected at a high percentage (19/24 women) in the biopsy tissues of the fimbrial and peritubal adhesions by in situ hybridization or immunoperoxidase stain, suggesting a persistent infection in these women even after antibiotic treatment.

journal_name

Am J Obstet Gynecol

authors

Patton DL,Askienazy-Elbhar M,Henry-Suchet J,Campbell LA,Cappuccio A,Tannous W,Wang SP,Kuo CC

doi

10.1016/s0002-9378(94)70084-2

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

95-101

issue

1

eissn

0002-9378

issn

1097-6868

pii

0002-9378(94)90453-7

journal_volume

171

pub_type

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