Vitamin E deficiency and aging effect on expression levels of GAP-43 and MAP-2 in selected areas of the brain.

Abstract:

:The expression levels of GAP-43 and MAP-2, two proteins involved, respectively, in axonal and dendritic remodeling, in control adult (11 months), old (24 months), and vitamin E-deficient (11 months) rats were evaluated. mRNA levels were determined by means of a quantitative in situ hybridization procedure in subregions of hippocampus and cerebellum. Though a general trend can be observed indicating a reduction in GAP-43 expression in aging as compared to adult animals and an increase in vitamin E-deprived rats in comparison with adult animals, no statistically significant change was found in any region analyzed. In the same way, MAP-2 mRNA levels show an increase in vitamin E-deprived rats in comparison with other groups tested; only one variation was statistically significant, namely the increase in cerebellar cortex MAP-2 nRNA levels in vitamin E-deficient versus adult rats. These results suggest that oxidative stress and aging negatively affect neuroplasticity, showing different characteristics at the dendritic and axonal levels.

journal_name

Ann N Y Acad Sci

authors

Casoli T,Di Stefano G,Delfino A,Fattoretti P,Bertoni-Freddari C

doi

10.1196/annals.1297.008

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

37-40

eissn

0077-8923

issn

1749-6632

pii

1019/1/37

journal_volume

1019

pub_type

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