Enhanced transcription of the human alpha 2A-adrenergic receptor gene by cAMP: evidence for multiple cAMP responsive sequences in the promoter region of this gene.


:Expression of the human alpha 2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5'-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative "core sequence" homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the alpha 2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.


Cell Signal


Cellular signalling


Shilo L,Sakaue M,Thomas JM,Philip M,Hoffman BB




Has Abstract


1994-01-01 00:00:00














  • Lysyl oxidase-like 2 (LOXL2) controls tumor-associated cell proliferation through the interaction with MARCKSL1.

    abstract::Lysyl oxidase-like 2 (LOXL2) is a member of the lysyl oxidase gene family that contributes to the invasiveness and metastasis in tumor progression. However, the role of LOXL2 in cellular signaling is incompletely understood. In this study, we investigated a possible mechanism of LOXL2 function in tumor metastases in v...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Kim BR,Dong SM,Seo SH,Lee JH,Lee JM,Lee SH,Rho SB

    更新日期:2014-09-01 00:00:00

  • The permissive effect of p21(Waf1/Cip1) on DNA synthesis is dependent on cell type: effect is absent in p53-inactive cells.

    abstract::The cyclin-dependent kinase inhibitors (CKI) interact with cyclin-cdk complexes to arrest mitogen-stimulated transit through the cell cycle, but we and others have recently shown that these molecules can exert permissive effects on cell cycle transit as well. The p53 protein induces transcription of the p21(Waf1/Cip1)...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Weiss RH,Randour CJ

    更新日期:2000-06-01 00:00:00

  • GADS is required for TCR-mediated calcium influx and cytokine release, but not cellular adhesion, in human T cells.

    abstract::GRB2 related adaptor protein downstream of Shc (GADS) is a member of the GRB2 family of adaptors and is critical for TCR-induced signaling. The current model is that GADS recruits SLP-76 to the LAT complex, which facilitates the phosphorylation of SLP-76, the activation of PLC-γ1, T cell adhesion and cytokine producti...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Bilal MY,Zhang EY,Dinkel B,Hardy D,Yankee TM,Houtman JC

    更新日期:2015-04-01 00:00:00

  • Protein kinase C-alpha and -beta play antagonistic roles in the differentiation process of THP-1 cells.

    abstract::The roles of protein kinase C (PKC) isoenzymes in the differentiation process of THP-1 cells are investigated. Inhibition of PKC by RO 31-8220 reduces the phagocytosis of latex particles and the release of superoxide, prostaglandin E(2) (PGE(2)), and tumour necrosis factor (TNF)-alpha. The proliferation of THP-1 cells...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Dieter P,Schwende H

    更新日期:2000-05-01 00:00:00

  • Apelin protects against liver X receptor-mediated steatosis through AMPK and PPARα in human and mouse hepatocytes.

    abstract::Non-alcoholic fatty liver disease is the most commonly occurring chronic liver disease, and hepatic steatosis, a condition defined as extensive lipid accumulation in hepatocytes, is associated with liver dysfunction and metabolic diseases, such as, obesity and type II diabetes. Apelin is an adipokine that acts on a G ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Huang J,Kang S,Park SJ,Im DS

    更新日期:2017-11-01 00:00:00

  • JIP4 is a PLK1 binding protein that regulates p38MAPK activity in G2 phase.

    abstract::Cell cycle progression from G2 phase into mitosis is regulated by a complex network of mechanisms, all of which finally control the timing of Cyclin B/CDK1 activation. PLK1 regulates a network of events that contribute to regulating G2/M phase progression. Here we have used a proteomics approach to identify proteins t...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Pinder A,Loo D,Harrington B,Oakes V,Hill MM,Gabrielli B

    更新日期:2015-11-01 00:00:00

  • Nitric oxide selectively inhibits adenylyl cyclase isoforms 5 and 6.

    abstract::We have previously shown that N18TG2 neuroblastoma cells express the type 6 adenylyl cyclase and that preincubation with nitric oxide (NO) attenuates Gs- and forskolin-stimulated activity. Here we show that this inhibition reflects a direct action of NO on the adenylyl cyclase. Preincubation of N18TG2 cell membranes a...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Hill J,Howlett A,Klein C

    更新日期:2000-04-01 00:00:00

  • Molecular insights into connective tissue growth factor action in rat pancreatic stellate cells.

    abstract::Pancreatic fibrosis, a key feature of chronic pancreatitis and pancreatic cancer, is mediated by activated pancreatic stellate cells (PSC). Connective tissue growth factor (CTGF) has been suggested to play a major role in fibrogenesis by enhancing PSC activation after binding to alpha5beta1 integrin. Here, we have foc...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Karger A,Fitzner B,Brock P,Sparmann G,Emmrich J,Liebe S,Jaster R

    更新日期:2008-10-01 00:00:00

  • Differential regulation of RhoA-mediated signaling by the TPalpha and TPbeta isoforms of the human thromboxane A2 receptor: independent modulation of TPalpha signaling by prostacyclin and nitric oxide.

    abstract::In humans, thromboxane (TX) A(2) signals through the TPalpha and TPbeta isoforms of the TXA(2) receptor that exhibit common and distinct roles. For example, Gq/phospholipase (PL)Cbeta signaling by TPalpha is directly inhibited by the vasodilators prostacyclin and nitric oxide (NO) whereas that signaling by TPbeta is u...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wikström K,Kavanagh DJ,Reid HM,Kinsella BT

    更新日期:2008-08-01 00:00:00

  • The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling.

    abstract::Previous reports have suggested that the two mitogen-activated protein kinases (MAPKs) in Dictyostelium discoideum, ERK1 and ERK2, can be directly activated in response to external cAMP even though these MAPKs play different roles in the developmental life cycle. To better characterize MAPK regulation, the levels of p...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Schwebs DJ,Hadwiger JA

    更新日期:2015-01-01 00:00:00

  • Induction of autophagy under nitrosative stress: A complex regulatory interplay between SIRT1 and AMPK in MCF7 cells.

    abstract::Induction of nitrosative stress has been observed in various cancer types and in tumor environment. However, it is still unclear how cancer cells combat the effect of nitrosative stress. The main targets of nitrosative stress in cells are cellular lipids, proteins and DNA. Autophagy or self-cleaning generates energy f...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Chakraborty S,Datta S,Ghosh S

    更新日期:2019-12-01 00:00:00

  • Staphylococcus aureus alpha-toxin activates phospholipases and induces a Ca2+ influx in PC12 cells.

    abstract::Staphylococcal alpha-toxin at subcytotoxic concentrations stimulated phosphatidylinositol turnover and arachidonic acid release in undifferentiated cultures of pheochromocytoma PC12 cells. Stimulation of phospholipase A2 but not C was dependent on extracellular calcium. Addition of staphylococcal alpha-toxin to PC12 c...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Fink D,Contreras ML,Lelkes PI,Lazarovici P

    更新日期:1989-01-01 00:00:00

  • Involvement of glycogen synthase kinase-3beta in hydrogen peroxide-induced suppression of Tcf/Lef-dependent transcriptional activity.

    abstract::Hydrogen peroxide (H(2)O(2)) mediates induction of cytotoxicity in various cell types. GSK-3beta has been found to participate in a number of signaling pathways, including cell proliferation and cell death. In the present study, we show that GSK-3beta is rapidly dephosphorylated and activated in response to H(2)O(2) t...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Shin SY,Chin BR,Lee YH,Kim JH

    更新日期:2006-05-01 00:00:00

  • Suppression of adenylyl cyclase-mediated cAMP production by plasma membrane associated cytoskeletal protein 4.1G.

    abstract::It has been shown lately that activity of G protein-coupled receptors (GPCRs) is regulated by an array of proteins binding to carboxy (C)-terminus of GPCRs. Proteins of 4.1 family are subsets of subcortical cytoskeletal proteins and are known to stabilize cellular structures and proteins at the plasma membrane. One of...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Goto T,Chiba A,Sukegawa J,Yanagisawa T,Saito M,Nakahata N

    更新日期:2013-03-01 00:00:00

  • Unsaturated fatty acids synergistically enhance glucocorticoid-induced gene expression.

    abstract::Regulation by unsaturated fatty acids of glucocorticoid-sensitive gene transcription was studied in HeLa cells transiently transfected with a mouse mammary tumour virus-luciferase reporter gene. Arachidonic acid and docosahexaenoic acid by themselves had no effect on basal levels of luciferase expression. However, the...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Vallette G,Sumida C,Thobie N,Nunez EA

    更新日期:1995-05-01 00:00:00

  • The signalling profile of recombinant human orexin-2 receptor.

    abstract::Orexin-A and orexin-B orchestrate their diverse central and peripheral effects via two G-protein coupled receptors, OX1R and OX2R, which activate multiple G-proteins. In many tissues, orexins activate extracellular signal-regulated kinase (ERK(1/2)) and p38 mitogen-activated protein kinase (MAPK); however, the mechani...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Tang J,Chen J,Ramanjaneya M,Punn A,Conner AC,Randeva HS

    更新日期:2008-09-01 00:00:00

  • RalGDS family members couple Ras to Ral signalling and that's not all.

    abstract::Ras proteins function as molecular switches that are activated in response to signalling pathways initiated by various extracellular stimuli and subsequently bind to numerous effector proteins leading to the activation of several signalling cascades within the cell. Ras and Ras-related proteins belong to a large super...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Ferro E,Trabalzini L

    更新日期:2010-12-01 00:00:00

  • Bacillus spore germination: Knowns, unknowns and what we need to learn.

    abstract::How might a microbial cell that is entirely metabolically dormant - and which has the ability to remain so for extended periods of time - irreversibly commit itself to resuming vegetative growth within seconds of being exposed to certain amino acids or sugars? That this process takes place in the absence of any detect...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Christie G,Setlow P

    更新日期:2020-10-01 00:00:00

  • Tyrosine phosphatase PTP1B modulates store-operated calcium influx.

    abstract::We have studied modulation of "store-operated calcium influx" by tyrosine phosphatases in the pancreatic acinar cell line AR42J and in HEK 293 cells. We show that inhibition of tyrosine phosphatases by bis-(N,N-dimethyl-hydroxamido) hydrooxovanadate (DMHV) leads to an increase in Ca(2+) release-activated Ca(2+) (CRAC)...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Hsu S,Schmid A,Sternfeld L,Anderie I,Solis G,Hofer HW,Schulz I

    更新日期:2003-12-01 00:00:00

  • Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.

    abstract::Interleukin 1 (IL-1) triggers the internalization of its cognate receptor from the plasma membrane. We recently demonstrated that this internalization is of critical importance for the IL-1-induced gene expression. In this study we report that the IL-1-induced activation of the small GTPase Rac1 requires receptor endo...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Windheim M,Hansen B

    更新日期:2014-01-01 00:00:00

  • Pertussis toxin-sensitive Gi/o proteins are involved in nerve growth factor-induced pro-survival Akt signaling cascade in PC12 cells.

    abstract::In Galpha(z)-deficient mice, survival of sympathetic neurons is significantly attenuated in the presence of pertussis toxin (PTX). This suggests that G(i/o) proteins may have distinct roles in neuronal survival. Here, we investigated the possible involvement of G(i/o) proteins in nerve growth factor (NGF)-induced pro-...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Wu EH,Wong YH

    更新日期:2005-07-01 00:00:00

  • Akt and Rac1 signaling are jointly required for insulin-stimulated glucose uptake in skeletal muscle and downregulated in insulin resistance.

    abstract::Skeletal muscle plays a major role in regulating whole body glucose metabolism. Akt and Rac1 are important regulators of insulin-stimulated glucose uptake in skeletal muscle. However the relative role of each pathway and how they interact are not understood. Here we delineate how Akt and Rac1 pathways signal to increa...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Sylow L,Kleinert M,Pehmøller C,Prats C,Chiu TT,Klip A,Richter EA,Jensen TE

    更新日期:2014-02-01 00:00:00

  • Constitutive serum response factor activation by the viral chemokine receptor homologue pUS28 is differentially regulated by Galpha(q/11) and Galpha(16).

    abstract::Expression of the human cytomegalovirus (HCMV)-encoded chemokine receptor homologue pUS28 in mammalian cells results in ligand-dependent and -independent changes in the activity of multiple cellular signal transduction pathways. The ligand-dependent signalling activity of pUS28 has been shown to be predominantly media...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Moepps B,Tulone C,Kern C,Minisini R,Michels G,Vatter P,Wieland T,Gierschik P

    更新日期:2008-08-01 00:00:00

  • A soluble LAT deletion mutant inhibits T-cell activation: reduced recruitment of signalling molecules to glycolipid-enriched microdomains.

    abstract::The type III transmembrane adaptor protein linker for activation of T cells (LAT) is essential for membrane recruitment of signalling molecules following TCR activation. Here we show that although LAT deleted in the transmembrane domain is completely soluble, it can be tyrosine phosphorylated after anti-CD3 stimulatio...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Torgersen KM,Vaage JT,Rolstad B,Taskén K

    更新日期:2001-03-01 00:00:00

  • Activation of MAP kinases in growth responsive pancreatic cancer cells.

    abstract::The implication of MAP kinases in the proliferation control of pancreatic cancer cells is still unknown. This study was undertaken to examine the contribution of the p44/p42 and p38 MAP kinases in the mitogenic response to epidermal growth factor (EGF) and bombesin in human pancreatic cancer cells, MIA PaCa-2 and PANC...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Douziech N,Calvo E,Lainé J,Morisset J

    更新日期:1999-08-01 00:00:00

  • GINS complex protein Sld5 recruits SIK1 to activate MCM helicase during DNA replication.

    abstract::In eukaryotes, proper loading and activation of MCM helicase at chromosomal origins plays a central role in DNA replication. Activation of MCM helicase requires its association with CDC45-GINS complex, but the mechanism of how this complex activates MCM helicase is poorly understood. Here we identified SIK1 (salt-indu...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Joshi K,Shah VJ,Maddika S

    更新日期:2016-12-01 00:00:00

  • Insulin activates caspase-3 by a phosphatidylinositol 3'-kinase-dependent pathway.

    abstract::Activation of the caspase proteases by c-Jun N-terminal kinase 1 (JNK1) has been proposed as a mechanism of apoptotic cell death. Here we report that insulin activates caspase-3 by a pathway requiring phosphatidylinositol 3'-kinase (PI3-kinase). JNK1 assays demonstrated that insulin treatment of myeloma cells induced ...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Godbout JP,Cengel KA,Cheng SL,Minshall C,Kelley KW,Freund GG

    更新日期:1999-01-01 00:00:00

  • Protein tyrosine phosphorylation as a mechanism of signalling in mast cells and basophils.

    abstract::Tyrosine phosphorylation of proteins is a mechanism of signalling for different receptors and is important for cell growth and differentiation. Mast cells and basophils are secretory cells that play a role in inflammatory and immediate allergic reactions. The activation/aggregation of different surface receptors on th...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: Hamawy MM,Mergenhagen SE,Siraganian RP

    更新日期:1995-08-01 00:00:00

  • Imatinib exhibit synergistic pleiotropy in the prevention of colorectal cancer by suppressing proinflammatory, cell survival and angiogenic signaling.

    abstract::Recent global incidences and mortality rates have placed colorectal cancer (CRC) at third and second positions, respectively, among both sexes of all ages. Resistance during chemotherapy is a big problem in the treatment and disease-free survival of CRC patients. Discovery of new anticancer drug(s) is a time taking pr...

    journal_title:Cellular signalling

    pub_type: 杂志文章


    authors: Dhiman DK,Sanyal SN,Vaish V

    更新日期:2020-12-01 00:00:00

  • Frizzled7 as an emerging target for cancer therapy.

    abstract::Wnt proteins are secreted glycoproteins that bind to the N-terminal extra-cellular cysteine-rich domain of the Frizzled (Fzd) receptor family. The Fzd receptors can respond to Wnt proteins in the presence of Wnt co-receptors to activate the canonical and non-canonical Wnt pathways. Recent studies indicated that, among...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审


    authors: King TD,Zhang W,Suto MJ,Li Y

    更新日期:2012-04-01 00:00:00