Enhanced transcription of the human alpha 2A-adrenergic receptor gene by cAMP: evidence for multiple cAMP responsive sequences in the promoter region of this gene.

Abstract:

:Expression of the human alpha 2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5'-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative "core sequence" homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the alpha 2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Shilo L,Sakaue M,Thomas JM,Philip M,Hoffman BB

doi

10.1016/0898-6568(94)90062-0

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

73-82

issue

1

eissn

0898-6568

issn

1873-3913

pii

0898-6568(94)90062-0

journal_volume

6

pub_type

杂志文章
  • The effect of oxidized low density lipoprotein (oxLDL)-induced heme oxygenase-1 on LPS-induced inflammation in RAW 264.7 macrophage cells.

    abstract::Macrophages take up oxidized low density lipoprotein (oxLDL) after being exposed to it in the blood vessels. oxLDL transforms macrophages into foam cells, which are a hallmark of atherosclerosis. The effects that oxLDL have on the inflammatory responses of foam cells are not clear. Here, we investigated how oxLDL modu...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2012.02.001

    authors: Min KJ,Cho KH,Kwon TK

    更新日期:2012-06-01 00:00:00

  • E3 ubiquitin ligase SIAH1 mediates ubiquitination and degradation of TRB3.

    abstract::Tribbles 3 homolog (TRB3) is recently identified as a scaffold-like regulator of various signal transducers and has been implicated in several processes including insulin signaling, NF-kappaB signaling, lipid metabolism and BMP signaling. To further understand cellular mechanisms of TRB3 regulation, we performed a yea...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2008.01.010

    authors: Zhou Y,Li L,Liu Q,Xing G,Kuai X,Sun J,Yin X,Wang J,Zhang L,He F

    更新日期:2008-05-01 00:00:00

  • Capsaicin induces cytotoxicity in pancreatic neuroendocrine tumor cells via mitochondrial action.

    abstract::Capsaicin (CAP), the pungent ingredient of chili peppers, inhibits growth of various solid cancers via TRPV1 as well as TRPV1-independent mechanisms. Recently, we showed that TRPV1 regulates intracellular calcium level and chromogranin A secretion in pancreatic neuroendocrine tumor (NET) cells. In the present study, w...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.09.014

    authors: Skrzypski M,Sassek M,Abdelmessih S,Mergler S,Grötzinger C,Metzke D,Wojciechowicz T,Nowak KW,Strowski MZ

    更新日期:2014-01-01 00:00:00

  • Bacillus spore germination: Knowns, unknowns and what we need to learn.

    abstract::How might a microbial cell that is entirely metabolically dormant - and which has the ability to remain so for extended periods of time - irreversibly commit itself to resuming vegetative growth within seconds of being exposed to certain amino acids or sugars? That this process takes place in the absence of any detect...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2020.109729

    authors: Christie G,Setlow P

    更新日期:2020-10-01 00:00:00

  • NEDD4 is overexpressed in colorectal cancer and promotes colonic cell growth independently of the PI3K/PTEN/AKT pathway.

    abstract::Ubiquitination controls multiple cellular processes relevant to cancer pathogenesis. Using Gene Set Enrichment Analysis of an mRNA transcriptome dataset, we have identified genes encoding components of the ubiquitin system that are differentially expressed in colorectal cancers as compared to normal colonic mucosa. Am...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2012.08.012

    authors: Eide PW,Cekaite L,Danielsen SA,Eilertsen IA,Kjenseth A,Fykerud TA,Ågesen TH,Bruun J,Rivedal E,Lothe RA,Leithe E

    更新日期:2013-01-01 00:00:00

  • Frizzled7 as an emerging target for cancer therapy.

    abstract::Wnt proteins are secreted glycoproteins that bind to the N-terminal extra-cellular cysteine-rich domain of the Frizzled (Fzd) receptor family. The Fzd receptors can respond to Wnt proteins in the presence of Wnt co-receptors to activate the canonical and non-canonical Wnt pathways. Recent studies indicated that, among...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2011.12.009

    authors: King TD,Zhang W,Suto MJ,Li Y

    更新日期:2012-04-01 00:00:00

  • The effect of lentivirus-mediated SIRT1 gene knockdown in the ATDC5 cell line via inhibition of the Wnt signaling pathway.

    abstract::SIRT1 is a highly conserved type III acetyltransferase gene located on chromosome 10 in mammals that belong to the Sirtuins family. In order to explore the effects of the SIRT1 gene in the ATDC5 cell line, an RNAi SIRT1 target sequence was designed and synthesized, aimed to knockdown the expression of SIRT1 in ATDC5 b...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2018.09.016

    authors: Yu F,Yuan Y,Li D,Kou Y,Jiang B,Zhang P

    更新日期:2019-01-01 00:00:00

  • Lysophosphatidic acid-induced arterial wall remodeling: requirement of PPARgamma but not LPA1 or LPA2 GPCR.

    abstract::Lysophosphatidic acid (LPA) and its ether analog alkyl-glycerophosphate (AGP) elicit arterial wall remodeling when applied intralumenally into the uninjured carotid artery. LPA is the ligand of eight GPCRs and the peroxisome proliferator-activated receptor gamma (PPARgamma). We pursued a gene knockout strategy to iden...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2009.08.003

    authors: Cheng Y,Makarova N,Tsukahara R,Guo H,Shuyu E,Farrar P,Balazs L,Zhang C,Tigyi G

    更新日期:2009-12-01 00:00:00

  • Regulation of acetylcholine receptor desensitization in mouse myotubes by cytosolic cyclic AMP.

    abstract::Whole-cell currents activated by bath applications of acetylcholine (ACh) (10-30 microM) were recorded from patch-clamped myotubes of the mouse C2 cell line. Increasing concentrations of forskolin caused a dose-dependent fast decay of ACh-activated currents as compared to the long-lasting ACh-currents in control cells...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(90)90064-h

    authors: Giovannelli A,Farini D,Gauzzi MC,Alema S,Eusebi F

    更新日期:1990-01-01 00:00:00

  • MyD88, IRAK1 and TRAF6 knockdown in human chondrocytes inhibits interleukin-1-induced matrix metalloproteinase-13 gene expression and promoter activity by impairing MAP kinase activation.

    abstract::Interleukin-1 (IL-1) is the major prototypic proinflammatory cytokine that stimulates degradation of cartilage in arthritis by inducing prominent collagen II-degrading matrix metalloproteinase-13 (MMP-13). Nothing is known about the involvement of adaptor proteins, MyD88, IRAK1 and TRAF6 in MMP-13 regulation. Here we ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2007.08.013

    authors: Ahmad R,Sylvester J,Zafarullah M

    更新日期:2007-12-01 00:00:00

  • Natural ligands of PPARgamma: are prostaglandin J(2) derivatives really playing the part?

    abstract::The peroxisome proliferator-activated receptor (PPAR) family was discovered from an orphan nuclear receptor approach, and thereafter, three subtypes were identified, namely PPARalpha, PPARbeta or PPARgamma and PPARgamma. The two former seem to regulate lipid homeostasis, whereas the latter is involved, among others, i...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/s0898-6568(01)00281-9

    authors: Nosjean O,Boutin JA

    更新日期:2002-07-01 00:00:00

  • Akt signalling in health and disease.

    abstract::Akt (also known as protein kinase B or PKB) comprises three closely related isoforms Akt1, Akt2 and Akt3 (or PKBα/β/γ respectively). We have a very good understanding of the mechanisms by which Akt isoforms are activated by growth factors and other extracellular stimuli as well as by oncogenic mutations in key upstrea...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2011.05.004

    authors: Hers I,Vincent EE,Tavaré JM

    更新日期:2011-10-01 00:00:00

  • Amino acid-dependent NPRL2 interaction with Raptor determines mTOR Complex 1 activation.

    abstract::We assign a new function to a tumor suppressor NPRL2 that activates the mTOR complex 1 (mTORC1) activity. The positive regulation of mTORC1 activity by NPRL2 is mediated through NPRL2 interaction with Raptor. While NPRL2 interacts with Rag GTPases, RagD in particular, to interfere with mTORC1 activity in amino acid sc...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2015.11.008

    authors: Kwak SS,Kang KH,Kim S,Lee S,Lee JH,Kim JW,Byun B,Meadows GG,Joe CO

    更新日期:2016-02-01 00:00:00

  • Syncytin-1 modulates placental trophoblast cell proliferation by promoting G1/S transition.

    abstract::Placental syncytiotrophoblasts formed by the fusion of cytotrophoblasts constitute the interface between maternal and fetal circulations. The syncytium, composed of a continuous layer of syncytiotrophoblasts, assumes the fetal-maternal nutrient exchange, placental barrier, and endocrine functions important for the mai...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.01.008

    authors: Huang Q,Li J,Wang F,Oliver MT,Tipton T,Gao Y,Jiang SW

    更新日期:2013-04-01 00:00:00

  • Cyclic AMP modulates interleukin-1 action in a cytotoxic T-cell hybridoma.

    abstract::The induction of cytolytic activity in PC60, a murine T-cell hybridoma, is paralleled by a rise in the level of BLT-esterase (N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl esterase), a serine esterase specific for activated T-cells. Both interleukin-1 (IL-1) and dibutyryl cAMP were albe to increase the esterase activi...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(90)90045-c

    authors: Schlegel-Haueter SE,Aebischer F

    更新日期:1990-01-01 00:00:00

  • Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.

    abstract::SCF induces autophosphorylation of Kit and activates a variety of signaling components including Jnks, Erks, PI 3 Kinase, the JAK-Stat pathway and members of the Src family. Previously we showed that Lyn is activated at multiple points during SCF-induced cell cycle progression and contributes to SCF-mediated growth, c...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2004.06.004

    authors: Shivakrupa R,Linnekin D

    更新日期:2005-01-01 00:00:00

  • The signalling profile of recombinant human orexin-2 receptor.

    abstract::Orexin-A and orexin-B orchestrate their diverse central and peripheral effects via two G-protein coupled receptors, OX1R and OX2R, which activate multiple G-proteins. In many tissues, orexins activate extracellular signal-regulated kinase (ERK(1/2)) and p38 mitogen-activated protein kinase (MAPK); however, the mechani...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2008.05.010

    authors: Tang J,Chen J,Ramanjaneya M,Punn A,Conner AC,Randeva HS

    更新日期:2008-09-01 00:00:00

  • Leptin upregulates VEGF in breast cancer via canonic and non-canonical signalling pathways and NFkappaB/HIF-1alpha activation.

    abstract::High levels of VEGF and leptin are strongly linked to worse prognosis of breast cancer. Leptin signalling upregulates VEGF in human and mouse mammary tumor cells (MT), but the specific molecular mechanisms are largely unknown. Pharmacologic and genetic approaches were used to dissect the mechanism of leptin regulation...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2010.05.003

    authors: Gonzalez-Perez RR,Xu Y,Guo S,Watters A,Zhou W,Leibovich SJ

    更新日期:2010-09-01 00:00:00

  • Zfra is an inhibitor of Bcl-2 expression and cytochrome c release from the mitochondria.

    abstract::Zfra is a small size 31-amino-acid C2H2 zinc finger-like protein, which is known to interact with c-Jun N-terminal kinase 1 (JNK1), WW domain-containing oxidoreductase (WWOX, FOR or WOX1), TNF receptor-associated death domain protein (TRADD) and nuclear factor kappaB (NF-kappaB) during stress response. Here, we show t...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2008.02.018

    authors: Hsu LJ,Hong Q,Schultz L,Kuo E,Lin SR,Lee MH,Lin YS,Chang NS

    更新日期:2008-07-01 00:00:00

  • Multifaceted transforming growth factor-beta (TGFβ) signalling in glioblastoma.

    abstract::Glioblastoma (GBM) is an aggressive and devastating primary brain cancer which responds very poorly to treatment. The average survival time of patients is only 14-15 months from diagnosis so there is a clear and unmet need for the development of novel targeted therapies to improve patient outcomes. The multifunctional...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2020.109638

    authors: Birch JL,Coull BJ,Spender LC,Watt C,Willison A,Syed N,Chalmers AJ,Hossain-Ibrahim MK,Inman GJ

    更新日期:2020-08-01 00:00:00

  • Flightless-1, a novel transcriptional modulator of PPARγ through competing with RXRα.

    abstract::Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor family and plays key roles in glucose and lipid metabolism. Its transcriptional control of target genes is mediated by ligand-dependent recruitment of coactivators. In this study, we demonstrate that a novel transcriptional modula...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.11.035

    authors: Choi JS,Choi SS,Kim ES,Seo YK,Seo JK,Kim EK,Suh PG,Choi JH

    更新日期:2015-03-01 00:00:00

  • Cellular signaling in pseudoxanthoma elasticum: an update.

    abstract::Pseudoxanthoma elasticum is an autosomal recessive genodermatosis with variable expression, due to mutations in the ABCC6 or ENPP1 gene. It is characterized by elastic fiber mineralization and fragmentation, resulting in skin, eye and cardiovascular symptoms. Significant advances have been made in the last 20 years wi...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2018.12.009

    authors: Van Gils M,Nollet L,Verly E,Deianova N,Vanakker OM

    更新日期:2019-03-01 00:00:00

  • A soluble LAT deletion mutant inhibits T-cell activation: reduced recruitment of signalling molecules to glycolipid-enriched microdomains.

    abstract::The type III transmembrane adaptor protein linker for activation of T cells (LAT) is essential for membrane recruitment of signalling molecules following TCR activation. Here we show that although LAT deleted in the transmembrane domain is completely soluble, it can be tyrosine phosphorylated after anti-CD3 stimulatio...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(01)00131-0

    authors: Torgersen KM,Vaage JT,Rolstad B,Taskén K

    更新日期:2001-03-01 00:00:00

  • An evaluation of strategies available for the identification of GTP-binding proteins required in intracellular signalling pathways.

    abstract::Strategies which can be used to elucidate the nature of a GTP-binding regulatory protein (G-protein) involved in an intracellular pathway of interest in the complex environment of the cell are described and evaluated. A desirable strategy is considered to be one in which the first stage indicates a requirement for one...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/s0898-6568(96)00131-3

    authors: Barritt GJ,Gregory RB

    更新日期:1997-05-01 00:00:00

  • Inducible cAMP early repressor (ICER) is a novel regulator of RIG-I mediated IFN-β production.

    abstract::Antiviral responses can be triggered by the cytoplasmic RNA helicase RIG-I that binds to viral RNA. RIG-I-mediated signaling stimulates the transcription factors IRF3 and NF-κB and their activation mechanisms have been intensively studied. Here we examined Sendai virus (SV)-mediated activation of the transcription fac...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.05.014

    authors: Johnsen IB,Bergstroem B,Stiberg KA,Thommesen L,Anthonsen MW

    更新日期:2013-09-01 00:00:00

  • Odorant-sensitive phospholipase C in insect antennae.

    abstract::Exogenous tritiated phosphatidylinositol bisphosphate added to antennal preparations from locust and cockroach was hydrolysed releasing inositol trisphosphate. High activity of phospholipase C was detected in the soluble as well as in the membrane fraction. At low free calcium concentrations hydrolysis of the labelled...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(90)90032-6

    authors: Boekhoff I,Strotmann J,Raming K,Tareilus E,Breer H

    更新日期:1990-01-01 00:00:00

  • Lithium enhances CRTC oligomer formation and the interaction between the CREB coactivators CRTC and CBP--implications for CREB-dependent gene transcription.

    abstract::Lithium salts are important drugs to treat bipolar disorder. Previous work showed that lithium by enforcing the interaction between the transcription factor CREB and its coactivator CRTC1 enhanced cAMP-stimulated CREB-dependent gene transcription. Both CREB and CRTC have been implicated in neuronal adaptation, which m...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2012.09.016

    authors: Heinrich A,von der Heyde AS,Böer U,Phu do T,Tzvetkov M,Oetjen E

    更新日期:2013-01-01 00:00:00

  • Distinct signalling properties of insulin receptor substrate (IRS)-1 and IRS-2 in mediating insulin/IGF-1 action.

    abstract::Insulin/IGF-1 action is driven by a complex and highly integrated signalling network. Loss-of-function studies indicate that the major insulin/IGF-1 receptor substrate (IRS) proteins, IRS-1 and IRS-2, mediate different biological functions in vitro and in vivo, suggesting specific signalling properties despite their h...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2018.03.003

    authors: Rabiee A,Krüger M,Ardenkjær-Larsen J,Kahn CR,Emanuelli B

    更新日期:2018-07-01 00:00:00

  • Multi-mechanisms are involved in reactive oxygen species regulation of mTORC1 signaling.

    abstract::The mammalian target of rapamycin complex 1(mTORC1) integrates diverse signals to control cell growth, proliferation, survival, and metabolism. Role of reactive oxygen species (ROS) on mTORC1 signaling remains obscure and mechanisms through which ROS modulate mTORC1 are not known.We demonstrate that low doses ROS expo...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2010.05.015

    authors: Li M,Zhao L,Liu J,Liu A,Jia C,Ma D,Jiang Y,Bai X

    更新日期:2010-10-01 00:00:00

  • Soluble and particulate inositol 1,4,5-trisphosphate 5-phosphatases show common antigenic determinants.

    abstract::Inositol 1,4,5-trisphosphate 5-phosphatase catalyses the dephosphorylation of the phosphate in the 5-position from inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate. One particulate and two soluble enzymes were previously described in bovine brain. In this study, we have obtained a precipitating anti...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(90)90082-l

    authors: Verjans B,Hollande F,Moreau C,Lejeune C,Erneux C

    更新日期:1990-01-01 00:00:00