Enhanced transcription of the human alpha 2A-adrenergic receptor gene by cAMP: evidence for multiple cAMP responsive sequences in the promoter region of this gene.

Abstract:

:Expression of the human alpha 2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5'-flanking sequences of the gene were placed in a promoterless vector with a chloramphenicol acetyltransferase (CAT) reporter gene, and cAMP-stimulated CAT activity was assayed in transfected JEG-3 placental carcinoma cells. Enhancer activity responsive to cAMP was located in DNA sequences both upstream and downstream from the endogenous promoter region. Within the upstream sequences there is a putative "core sequence" homologous to the eight base CRE consensus palindrome, but this region did not function independently as a CRE enhancer; additional upstream sequences were required to provide significant enhancer activity in response to cAMP. Regulation of expression of the alpha 2A-adrenergic gene by cAMP is complex and involves multiple and likely novel DNA sequences.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Shilo L,Sakaue M,Thomas JM,Philip M,Hoffman BB

doi

10.1016/0898-6568(94)90062-0

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

73-82

issue

1

eissn

0898-6568

issn

1873-3913

pii

0898-6568(94)90062-0

journal_volume

6

pub_type

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