The use of fed batch cultivation for achieving high cell densities in the production of a recombinant protein in Escherichia coli.


:The production of the fusion protein staphylococcal protein A/E. coli beta-galactosidase in Escherichia coli was studied in batch and fed batch cultivations. Batch cultivation of a recombinant E. coli strain yielded a final cell dry weight of 16.4 g l-1 with a final intracellular product concentration of recombinant protein corresponding to approximately 38% of the cell dry weight. Fed batch cultivation made it possible to increase the final cell dry weight to 77.0 g l-1. The intracellular product concentration (25%) was lower as compared to batch cultivation resulting in a total concentration of recombinant protein of 19.2 g l-1.


FEMS Microbiol Rev


Strandberg L,Andersson L,Enfors SO




Has Abstract


1994-05-01 00:00:00














  • Expression cloning of fungal enzyme genes; a novel approach for efficient isolation of enzyme genes of industrial relevance.

    abstract::Expression cloning is a relatively new method for fast and efficient cloning of enzyme genes from fungi that are known to make complex enzyme mixtures. In contrast to traditional cloning methods that are usually dependent on knowledge of at least a partial amino acid sequence in order to synthesize appropriate DNA pro...

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    pub_type: 杂志文章,评审


    authors: Dalbøge H

    更新日期:1997-08-01 00:00:00

  • Formate dehydrogenase.

    abstract::Formate is a substrate, or product, of diverse reactions catalyzed by eukaryotic organisms, eubacteria, and archaebacteria. A survey of metabolic groups reveals that formate is a common growth substrate, especially among the anaerobic eubacteria and archaebacteria. Formate also functions as an accessory reductant for ...

    journal_title:FEMS microbiology reviews

    pub_type: 杂志文章,评审


    authors: Ferry JG

    更新日期:1990-12-01 00:00:00

  • Post-transcriptional controls in bacteriophage T4: roles of the sequence-specific endoribonuclease RegB.

    abstract::Gene regB of bacteriophage T4 encodes a sequence-specific endoribonuclease which introduces cuts in early phage messenger RNAs. In most cases, cutting takes place in the middle of the tetranucleotide GGAG. Efficient cleavages occur in the motifs located in intergenic regions, some of them being Shine-Dalgarno sequence...

    journal_title:FEMS microbiology reviews

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    authors: Sanson B,Uzan M

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  • Applications of S-layers.

    abstract::The wealth of information existing on the general principle of S-layers has revealed a broad application potential. The most relevant features exploited in applied S-layer research are: (i) pores passing through S-layers show identical size and morphology and are in the range of ultrafiltration membranes; (ii) functio...

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    authors: Duda RL,Martincic K,Xie Z,Hendrix RW

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    pub_type: 杂志文章,评审


    authors: Ghildyal R,Ho A,Jans DA

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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


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    journal_title:FEMS microbiology reviews

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    authors: Rzezutka A,Cook N

    更新日期:2004-10-01 00:00:00

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    journal_title:FEMS microbiology reviews

    pub_type: 杂志文章,评审


    authors: Buvelot H,Posfay-Barbe KM,Linder P,Schrenzel J,Krause KH

    更新日期:2017-03-01 00:00:00

  • Microbial arsenic: from geocycles to genes and enzymes.

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    authors: Elias S,Banin E

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    authors: Mann NH

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    journal_title:FEMS microbiology reviews

    pub_type: 杂志文章,评审


    authors: Whitehead NA,Barnard AM,Slater H,Simpson NJ,Salmond GP

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  • Industrial production of heterologous proteins by fed-batch cultures of the yeast Saccharomyces cerevisiae.

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    journal_title:FEMS microbiology reviews

    pub_type: 杂志文章,评审


    authors: Mendoza-Vega O,Sabatié J,Brown SW

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    authors: Morgenstein RM,Szostek B,Rather PN

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    journal_title:FEMS microbiology reviews

    pub_type: 杂志文章,评审


    authors: Cinatl J Jr,Vogel JU,Kotchetkov R,Wilhelm Doerr H

    更新日期:2004-02-01 00:00:00