The use of fed batch cultivation for achieving high cell densities in the production of a recombinant protein in Escherichia coli.

Abstract:

:The production of the fusion protein staphylococcal protein A/E. coli beta-galactosidase in Escherichia coli was studied in batch and fed batch cultivations. Batch cultivation of a recombinant E. coli strain yielded a final cell dry weight of 16.4 g l-1 with a final intracellular product concentration of recombinant protein corresponding to approximately 38% of the cell dry weight. Fed batch cultivation made it possible to increase the final cell dry weight to 77.0 g l-1. The intracellular product concentration (25%) was lower as compared to batch cultivation resulting in a total concentration of recombinant protein of 19.2 g l-1.

journal_name

FEMS Microbiol Rev

authors

Strandberg L,Andersson L,Enfors SO

doi

10.1111/j.1574-6976.1994.tb00072.x

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

53-6

issue

1

eissn

0168-6445

issn

1574-6976

pii

0168-6445(94)90013-2

journal_volume

14

pub_type

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