Abstract:
:The production of the fusion protein staphylococcal protein A/E. coli beta-galactosidase in Escherichia coli was studied in batch and fed batch cultivations. Batch cultivation of a recombinant E. coli strain yielded a final cell dry weight of 16.4 g l-1 with a final intracellular product concentration of recombinant protein corresponding to approximately 38% of the cell dry weight. Fed batch cultivation made it possible to increase the final cell dry weight to 77.0 g l-1. The intracellular product concentration (25%) was lower as compared to batch cultivation resulting in a total concentration of recombinant protein of 19.2 g l-1.
journal_name
FEMS Microbiol Revjournal_title
FEMS microbiology reviewsauthors
Strandberg L,Andersson L,Enfors SOdoi
10.1111/j.1574-6976.1994.tb00072.xsubject
Has Abstractpub_date
1994-05-01 00:00:00pages
53-6issue
1eissn
0168-6445issn
1574-6976pii
0168-6445(94)90013-2journal_volume
14pub_type
杂志文章abstract::The increasing numbers of characterized base-pairing small RNAs (sRNAs) and the identification of these regulators in a broad range of bacteria are allowing comparisons between species and explorations of sRNA evolution. In this review, we describe some examples of trans-encoded base-pairing sRNAs that are species-spe...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fuv014
更新日期:2015-05-01 00:00:00
abstract::Many fungi produce exocellular beta-glucan-degrading enzymes, the beta-glucanases including the noncellulolytic beta-(1,3)- and beta-(1,6)-glucanases, degrading beta-(1,3)- and beta-(1,6)-glucans. An ability to purify several exocellular beta-glucanases attacking the same linkage type from a single fungus is common, a...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章
doi:10.1111/j.1574-6976.2006.00055.x
更新日期:2007-03-01 00:00:00
abstract::The spread of antibiotic resistance and increasing prevalence of biofilm-associated infections is driving demand for new means to treat bacterial infection. Nanotechnology provides an innovative platform for addressing this challenge, with potential to manage even infections involving multidrug-resistant (MDR) bacteri...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fux003
更新日期:2017-05-01 00:00:00
abstract::Ribonuclease P is the endonuclease required for generating the mature tRNA 5'-end. The ribonucleoprotein character of this enzyme has now been proven in most organisms and organelles. Exceptions, however, are still the chloroplasts, plant nuclei and animal mitochondria where no associated RNAs have been detected to da...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.1999.tb00406.x
更新日期:1999-06-01 00:00:00
abstract::Expression cloning is a relatively new method for fast and efficient cloning of enzyme genes from fungi that are known to make complex enzyme mixtures. In contrast to traditional cloning methods that are usually dependent on knowledge of at least a partial amino acid sequence in order to synthesize appropriate DNA pro...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.1997.tb00343.x
更新日期:1997-08-01 00:00:00
abstract::N-Substituted aromatic compounds are environmental contaminants associated with the production and use of dyes, explosives, pesticides and pharmaceuticals. In this article, we examine the potential of anaerobic granular sludge from anaerobic treatment systems towards the detoxification, transformation, and mineralizat...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章
doi:10.1111/j.1574-6976.1997.tb00335.x
更新日期:1997-07-01 00:00:00
abstract::Pathogenicity and symbiosis are central to bacteria-host interactions. Although several human pathogens have been subjected to functional genomic analysis, we still understand little about bacteria-invertebrate interactions despite their ecological prevalence. Advances in our knowledge of this area are often hindered ...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2003.tb00625.x
更新日期:2003-01-01 00:00:00
abstract::Understanding the structure, functionalities and biology of functional amyloids is an issue of emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria during protein production processes, have emerged as unanticipated, highly tunable models for the scrutiny of the physiology and arc...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fuy038
更新日期:2019-01-01 00:00:00
abstract::Thioredoxin is a small (Mr 12,000) ubiquitous redox protein with the conserved active site structure: -Trp-Cys-Gly-Pro-Cys-. The oxidized form (Trx-S2) contains a disulfide bridge which is reduced by NADPH and thioredoxin reductase; the reduced form [Trx(SH)2] is a powerful protein disulfide oxidoreductase. Thioredoxi...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6968.1988.tb02747.x
更新日期:1988-12-01 00:00:00
abstract::The initiation of transcription is the most important step for gene regulation in eubacteria. To initiate transcription, RNA polymerase has to associate with a small protein, known as a sigma-factor. The sigma-factor directs RNA polymerase to a specific class of promoter sequences. Most bacterial species synthesize se...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.1998.tb00364.x
更新日期:1998-09-01 00:00:00
abstract::Gene conversion is defined as the non-reciprocal transfer of information between homologous sequences. Despite methodological problems to establish non-reciprocity, gene conversion has been demonstrated in a wide variety of bacteria. Besides examples of high-frequency reversion of mutations in repeated genes, gene con...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1016/j.femsre.2004.10.004
更新日期:2005-04-01 00:00:00
abstract::The genus Rickettsia (Alphaproteobacteria, Rickettsiales, Rickettsiaceae) is comprised of obligate intracellular parasites, with virulent species of interest both as causes of emerging infectious diseases and for their potential deployment as bioterrorism agents. Currently, there are no effective commercially availabl...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/1574-6976.12084
更新日期:2015-01-01 00:00:00
abstract::Fungal mycelial by-products from fermentation industries present a considerable affinity for soluble metal ions (e.g. Zn, Cd, Ni, Pb, Cr, Ag) and could be used in biosorption processes for purification of contaminated effluents. In this work the influence of pH on sorption parameters is characterized by measuring the ...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章
doi:10.1111/j.1574-6976.1994.tb00106.x
更新日期:1994-08-01 00:00:00
abstract::Pseudomonas putida is a gram-negative rod-shaped gammaproteobacterium that is found throughout various environments. Members of the species P. putida show a diverse spectrum of metabolic activities, which is indicative of their adaptation to various niches, which includes the ability to live in soils and sediments con...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2010.00249.x
更新日期:2011-03-01 00:00:00
abstract::Antibiotic resistance mechanisms reported in Gram-negative bacteria are causing a worldwide health problem. The continuous dissemination of 'multidrug-resistant' (MDR) bacteria drastically reduces the efficacy of our antibiotic 'arsenal' and consequently increases the frequency of therapeutic failure. In MDR bacteria,...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2011.00290.x
更新日期:2012-03-01 00:00:00
abstract::Type II (proteic) toxin-antitoxin (TA) operons are widely spread in bacteria and archaea. They are organized as operons in which, usually, the antitoxin gene precedes the cognate toxin gene. The antitoxin generally acts as a transcriptional self-repressor, whereas the toxin acts as a co-repressor, both proteins consti...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fuv002
更新日期:2015-07-01 00:00:00
abstract::Nickel is an essential nutrient for selected microorganisms where it participates in a variety of cellular processes. Many microbes are capable of sensing cellular nickel ion concentrations and taking up this nutrient via nickel-specific permeases or ATP-binding cassette-type transport systems. The metal ion is specif...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1016/S0168-6445(03)00042-1
更新日期:2003-06-01 00:00:00
abstract::In recent years, there has been accumulating evidence that antibiotics, besides their antimicrobial action, potentially have a number of undesired side effects that can, at least in some cases, promote genetic variability of bacteria. In addition to resistant variants, antibiotics have also been shown to select mutato...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2009.00165.x
更新日期:2009-05-01 00:00:00
abstract::The bacterium Chlamydia trachomatis is the etiological agent of the most common sexually transmitted infection in North America and Europe. Medical complications resulting from genital C. trachomatis infections arise predominantly in women where the initial infections often remain asymptomatic and thus unrecognized. U...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fuw027
更新日期:2016-11-01 00:00:00
abstract::We review and update the work on genetic elements, e.g., viruses and plasmids (exluding IS elements and transposons) in the kingdom Crenarchaeota (Thermoproteales and Sulfolobales) and the orders Thermococcales and Thermoplasmales in the kingdom Euryarchaeota of the archael domain, including unpublished data from our ...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.1996.tb00239.x
更新日期:1996-05-01 00:00:00
abstract::Dengue is the most prevalent arthropod-borne viral disease, caused by dengue virus, a member of the Flaviviridae family. Its worldwide incidence is now a major health problem, with 2.5 billion people living in risk areas. In this review, we integrate the structural rearrangements of each viral protein and their functi...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fuu004
更新日期:2015-03-01 00:00:00
abstract::The species concept is a recurrent controversial issue that preoccupies philosophers as well as biologists of all disciplines. Prokaryotic species concept has its own history and results from a series of empirical improvements parallel to the development of the techniques of analysis. Among the microbial taxonomists, ...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2001.tb00571.x
更新日期:2001-01-01 00:00:00
abstract::Gene regB of bacteriophage T4 encodes a sequence-specific endoribonuclease which introduces cuts in early phage messenger RNAs. In most cases, cutting takes place in the middle of the tetranucleotide GGAG. Efficient cleavages occur in the motifs located in intergenic regions, some of them being Shine-Dalgarno sequence...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.1995.tb00196.x
更新日期:1995-08-01 00:00:00
abstract::Stress responses are crucial for bacteria to survive harmful conditions that they encounter in the environment. Although gene regulatory mechanisms underlying stress responses in bacteria have been thoroughly characterised for decades, recent advances in imaging technologies helped to uncover previously hidden dynamic...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章
doi:10.1093/femsre/fuaa022
更新日期:2020-09-01 00:00:00
abstract::Microbial networks are an increasingly popular tool to investigate microbial community structure, as they integrate multiple types of information and may represent systems-level behaviour. Interpreting these networks is not straightforward, and the biological implications of network properties are unclear. Analysis of...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fuy030
更新日期:2018-11-01 00:00:00
abstract::Phenotypically heterogeneous microenvironments emerge as biofilms mature across different environments. Phenotypic heterogeneity in biofilm sub-populations not obeying quorum sensing-dictated, collective group behavior may be considered as a strategy allowing non-conformists to survive hostile conditions. Heterogeneou...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1093/femsre/fuy001
更新日期:2018-05-01 00:00:00
abstract::For many years, the bacterial cells were regarded as tiny vessels lacking internal organization. This view, which stemmed from the scarcity of membrane-bounded organelles, has changed considerably in recent years, mainly due to advancements in imaging capabilities. Consequently, despite the rareness of conventional or...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2012.00348.x
更新日期:2012-09-01 00:00:00
abstract::Based on structural, biochemical, and genetic data, the soluble diiron monooxygenases can be divided into four groups: the soluble methane monooxygenases, the Amo alkene monooxygenase of Rhodococcus corallinus B-276, the phenol hydroxylases, and the four-component alkene/aromatic monooxygenases. The limited phylogenet...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1016/S0168-6445(03)00023-8
更新日期:2003-10-01 00:00:00
abstract::The accurate synthesis of proteins, dictated by the corresponding nucleotide sequence encoded in mRNA, is essential for cell growth and survival. Central to this process are the aminoacyl-tRNA synthetases (aaRSs), which provide amino acid substrates for the growing polypeptide chain in the form of aminoacyl-tRNAs. The...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2008.00119.x
更新日期:2008-07-01 00:00:00
abstract::In the last decade, a variety of ribosomally synthesized antimicrobial peptides or bacteriocins produced by lactic acid bacteria have been identified and characterized. As a result of these studies, insight has been gained into fundamental aspects of biology and biochemistry such as producer self protection, membrane-...
journal_title:FEMS microbiology reviews
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6976.2000.tb00534.x
更新日期:2000-01-01 00:00:00