Abstract:
:Treatment of rat parotid acinar cells with 4 beta-phorbol 12-myristate 13-acetate (PMA) significantly inhibited an increase in cytosolic free Ca2+ concentration ([Ca2+]i) induced by carbachol (CCh), a muscarinic agonist. The CCh-induced increase in [Ca2+]i was also inhibited by another active phorbol ester, 4 beta-phorbol 12,13-dibutyrate, but not by 4 alpha-phorbol 12,13-didecanoate, which does not activate protein kinase C. The treatment with PMA had no effect on increases in [Ca2+]i evoked by ionomycin and thapsigargin, which do not stimulate phosphoinositide hydrolysis. In contrast, an increase in [Ca2+]i induced by NaF, a direct activator of GTP-binding proteins, was delayed in the presence of PMA. The formation of inositol phosphates in response to CCh was suppressed significantly by PMA treatment. In radioligand binding assays, PMA did not directly interfere with the specific binding of [3H]quinuclidinyl benzilate ([3H]QNB), a muscarinic antagonist, to plasma membranes. Furthermore, the [3H]QNB binding to plasma membranes prepared from the PMA-pretreated cells was not different from that to the control membranes. These results indicate that PMA attenuated the CCh-induced increase in [Ca2+]i through inhibition of phosphoinositide hydrolysis. Activation of protein kinase C may play a role in negative-feedback control of the muscarinic pathway in rat parotid acinar cells.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Tojyo Y,Tanimura A,Matsumoto Ydoi
10.1016/0006-2952(94)90081-7subject
Has Abstractpub_date
1994-06-01 00:00:00pages
2055-61issue
11eissn
0006-2952issn
1873-2968pii
0006-2952(94)90081-7journal_volume
47pub_type
杂志文章abstract::One feature that contraindicates the wide therapeutic use of retinoids is their teratogenicity. Synthetic retinoids are distinguishable from each other on the basis of their partial or exclusive preference in binding and activation of all-trans retinoic acid receptors (RARs) or retinoid X receptors (RXRs). Using mouse...
journal_title:Biochemical pharmacology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1985-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2017-01-15 00:00:00
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pub_type: 杂志文章
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更新日期:1994-07-05 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1989-05-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2000-12-15 00:00:00
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pub_type: 杂志文章
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更新日期:2004-02-15 00:00:00
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pub_type: 杂志文章
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更新日期:1998-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-09-01 00:00:00