Immunoaffinity purification of human haptoglobin using monoclonal antibodies.

Abstract:

:Two monoclonal antibodies, coupled to Sepharose 4B, were used for rapid isolation of the human haptoglobin in a single chromatographic step. The purity of haptoglobin was judged by SDS polyacrylamide gel electrophoresis, immunoblotting, immunoelectrophoresis and size exclusion high performance liquid chromatography. Moreover, the preparation showed ability to form active complexes with hemoglobin, Concanavalin A and specific antibody. Polyclonal goat antiserum produced with the obtained haptoglobin preparation formed one precipitin line with whole human serum. On the other hand, monoclonal antibodies can be purified by means of haptoglobin coupled to matrix.

authors

Katnik I,Jadach J

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

303-8

issue

5-6

eissn

0004-069X

issn

1661-4917

journal_volume

41

pub_type

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