Description of B lymphocytes and plasma cells, complement, and chemokines/receptors in acute liver allograft rejection.


BACKGROUND:Although antibody mechanisms play a pathogenetic role in liver allograft rejection, no data exist on B lymphocytes, plasma cells, complement, and chemokines in rejected liver tissue. METHODS:Liver biopsy specimens from 25 patients with acute allograft rejection (AR) (rejection activity index, RAI score: 1-9) were analyzed by immunohistochemistry (IH) and reverse transcriptase-polymerase chain reaction (RT-PCR) and compared with biopsy specimens taken prior to implantation (PI). The number of CD20 and CD138 cells was evaluated, and the presence and abundance of the chemokines macrophage inflammatory protein (MIP)-3alpha, CXCL9, CXCL10, CXCL11, CXCL12, and their receptors CCR-6, CXCR3, and CXCR4 were examined. Complement depositions were visualized by C4d IH. RESULTS:The numbers of B lymphocytes (P=0.002) and plasma cells (P=0.022) were significantly higher in AR biopsy specimens compared with PI biopsy specimens. MIP-3alpha and CCR-6 cells were detected in the portal fields of all AR biopsy specimens. IH double staining revealed a colocalization of MIP-3alpha/CD20 cells; C4d deposits could be demonstrated along the portal capillaries. All examined chemokines and receptors could be detected in normal liver tissue and in AR biopsy specimens by RT-PCR and semiquantitative RT-PCR, demonstrating an overexpression of CXCL10 and -11. CONCLUSIONS:The significant increase of B lymphocytes and plasma cells during acute rejection, together with the lack of a significant increase of proliferating cells, indicates that the migration of B lymphocytes and plasma cells-promoted by the expression of B-cell activating chemokines/receptors-plays a key role in acute liver rejection. The C4d deposits along the portal capillaries indicate a humorally mediated alloresponse caused by the accumulated B and plasma cells.






Krukemeyer MG,Moeller J,Morawietz L,Rudolph B,Neumann U,Theruvath T,Neuhaus P,Krenn V




Has Abstract


2004-07-15 00:00:00














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    pub_type: 杂志文章


    authors: van den Helder TB,Benhaim P,Anthony JP,McCalmont TH,Mathes SJ

    更新日期:1994-02-01 00:00:00

  • Outcomes of laparoscopic donor nephrectomy in obese patients.

    abstract::The applicability of laparoscopic donor nephrectomy (LDN) has not been assessed in the obese donor. We hypothesized that obesity is not a technical contraindication to LDN. From May 1998 to February 1999, 40 patients underwent LDN at the Georgetown Transplant Institute with the transperitoneal technique. Prophylaxis a...


    pub_type: 杂志文章


    authors: Kuo PC,Plotkin JS,Stevens S,Cribbs A,Johnson LB

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    更新日期:2010-12-27 00:00:00

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    authors: Korbutt GS,Rayat GR,Ezekowitz J,Rajotte RV

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    abstract:BACKGROUND:The role of asymptomatic bacteriuria in kidney transplant recipients is unknown. There is no clear evidence of its effect on transplanted kidney. METHODS:We studied urine cytokines profile among kidney transplant recipients with bacteriuria found in screening examination. Urine cultures were collected in 26...


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    authors: Ciszek M,Paczek L,Bartłomiejczyk I,Mucha K

    更新日期:2006-06-27 00:00:00

  • Time-response studies of the cellular immune response to cell membrane antigens.

    abstract::It was the objective of these experiments to study the time-related changes in the responsiveness of the cellular elements of the immune system following contact with single non-H-2 or multiple H-2 histocompatibility antigens. The reactivity of spleen cells from mice that received injections of spleen cells bearing H-...


    pub_type: 杂志文章


    authors: Graff RJ,Kurtz ME,Russell H,Martin-Morgan D

    更新日期:1988-02-01 00:00:00

  • Mechanisms of tolerance induced by PG490-88 in a bone marrow transplantation model.

    abstract:BACKGROUND:PG490-88, a semisynthetic derivative of a novel compound PG490 (triptolide) purified from a Chinese herb (Tripterygium wilfordii Hook F), is effective in prevention of murine graft-versus-host disease (GVHD). METHODS:PG490-88 was administrated into recipients in a model (B10.D2 [H2d, Mls-2b, Mls-3b]-->BALB/...


    pub_type: 杂志文章


    authors: Chen BJ,Chen Y,Cui X,Fidler JM,Chao NJ

    更新日期:2002-01-15 00:00:00

  • Infection of human endothelium in vitro by cytomegalovirus causes enhanced expression of purinergic receptors: a potential virus escape mechanism?

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    authors: Zandberg M,van Son WJ,Harmsen MC,Bakker WW

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    pub_type: 杂志文章


    authors: Hosenpud JD,Shipley GD,Morris TE,Hefeneider SH,Wagner CR

    更新日期:1993-02-01 00:00:00

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    authors: Motomura N,Lou H,Orskov H,Ramwell PW,Foegh ML

    更新日期:1998-04-27 00:00:00

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    authors: Van Thiel DH,Gavaler JS,Schade RR,Chien MC,Starzl TE

    更新日期:1992-07-01 00:00:00

  • Low pretransplant bone-mineral density and rapid bone loss do not increase risk for avascular osteonecrosis after allogeneic hematopoietic stem cell transplantation.

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    pub_type: 临床试验,杂志文章


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    authors: Lai JH,Ho LJ,Kwan CY,Chang DM,Lee TC

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    pub_type: 杂志文章


    authors: Fukuda M

    更新日期:1983-08-01 00:00:00

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    authors: van Dorp WT,Jonges E,Bruggeman CA,Daha MR,van Es LA,van Der Woude FJ

    更新日期:1989-09-01 00:00:00

  • HLA-DR and DQ typing by polymerase chain reaction using sequence-specific primer mixes reduces the incidence of phenotypic homozygosity (blanks) over serology.

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    authors: Bryan CF,Harrell KM,Nelson PW,Pierce GE,Ross G,Shield CF 3rd,Warady BA,Aeder MI,Helling TS,Landreneau MD,Luger AM

    更新日期:1996-12-27 00:00:00

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    authors: Omura T,Ascher NL,Emond JC

    更新日期:1996-07-27 00:00:00

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    authors: Kaminski ER,Kaminski A,Bending MR,Chang R,Heads A,Szydlo RM,Pereira RS

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    pub_type: 临床试验,杂志文章,随机对照试验


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    pub_type: 杂志文章


    authors: Nassiri M,Viciana A,Streilein JW,Ruiz P

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    pub_type: 杂志文章


    authors: Shahid M,Johnson J,Nightingale P,Neuberger J

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Garcia-Maldonado M,Kaufman CE,Comp PC

    更新日期:1991-03-01 00:00:00

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    pub_type: 临床试验,杂志文章


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    pub_type: 杂志文章


    authors: Burdick JF,Clow LW

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    pub_type: 杂志文章


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    pub_type: 杂志文章,随机对照试验


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    更新日期:2019-11-01 00:00:00

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    pub_type: 杂志文章


    authors: Tian Y,Rüdiger HA,Jochum W,Clavien PA

    更新日期:2002-11-15 00:00:00

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    pub_type: 杂志文章


    authors: Corry RJ,West JC,Hunsicker LG,Schanbacher BA,Lachenbruch PA

    更新日期:1980-12-01 00:00:00